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Danielle Kian,Cesar Armando Contreras Lanche,Igor Alexandre Campos Damiani,Tamiris Zanforlin Olmos Fernan,Phileno Pinge-Filho,Marcia Regina Machado dos Sant,Jose Franco da Silveira,Celso Vataru Nakamu 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.4
This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR were used for the transcripts. Besides, immunolocalization of recombinant protein was done by immunofluorescence and electron microscopy. Analysis indicated the presence of a single copy of Tc8.2 in the T. cruzi genome and 2-different sized transcripts in epimastigotes/amastigotes and trypomastigotes. Immunoblotting showed 70 and 80 kDa polypeptides in epimastigotes and trypomastigotes, respectively, and a differential pattern of immunolocalization. Overall, the results suggest that Tc8.2 is differentially expressed during the T. cruzi life cycle.
Y. Kiyanagi,K. Kino,M. Furusaka,F. Hiraga,T. Kamiyama,K. Kato,M. Igashira,T. Katabuchi,M. Mizumoto,M. Oshima,H. Harada,J. Katakura,K. Furutaka,S. Goko,A. Kimura,T. Kin,F. Kitatani,M. Koizumi,S. Nakamu 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
A project on the comprehensive nuclear data research for the development of the advanced reactor systems had been executed successfully by eight organizations from 2005 to 2009. In this project, we constructed a pulsed neutron beamline that was aimed to obtain neutron capture cross-sections of long-lived fission products and minor actinides accurately. The energy spectra, spatial distributions, and pulses of the beam were studied by measurements and simulation calculations, and they were found to be consistent with those of the beamline design. In this paper, we present the overview of the project and the properties of the neutron beam provided by this beam line.
Mechanistic Investigation of Water Oxidation Catalyzed by Uniform, Assembled MnO Nanoparticles
Jin, Kyoungsuk,Seo, Hongmin,Hayashi, Toru,Balamurugan, Mani,Jeong, Donghyuk,Go, Yoo Kyung,Hong, Jung Sug,Cho, Kang Hee,Kakizaki, Hirotaka,Bonnet-Mercier, Nadè,ge,Kim, Min Gyu,Kim, Sun Hee,Nakamu American Chemical Society 2017 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.139 No.6
<P>The development of active water oxidation catalysts is critical to achieve high efficiency in overall water splitting. Recently, sub 10 nm-sized monodispersed partially oxidized manganese oxide nanoparticles were shown to exhibit not only superior catalytic performance for oxygen evolution, but also unique electrokinetics, as compared to their bulk counterparts. In the present work, the water oxidizing mechanism of partially oxidized MnO nanoparticles was investigated using integrated in situ spectroscopic and electrokinetic analyses. We successfully demonstrated that, in contrast to previously reported manganese (Mn)-based catalysts, Mn(III) species are stably generated on the, surface of MnO nanoparticles via a proton-coupled electron transfer pathway. Furthermore, we confirmed as to MnO nanoparticles that the one-electron oxidation step from Mn(II) to Mn(III) is no longer the rate-determining step for water oxidation and that Mn(W)=O species are generated as reaction intermediates during catalysis.</P>