장생도라지의 약리작용

성낙주,서종권 경상대학교 농업자원이용연구소 1998 심포지엄 자료집 Vol.1998 No.1

도라지 (길경, Platycodon grandiflorum A. DC)는 예부터 천식, 기관지염 등에 효능이 인정되어 약용 및 식용으로 널리 이용되어 왔다. 장생 도라지에 대한 연구로 식이성 고혈압 흰쥐에 도라지 분말을 각 5%와 10% 함유한 식이를 3주간 급이하였을 때 혈청과 간장의 지질 농도가 현저하게 감소하였으며, 5%의 도라지 분말 함유 식이의 급이시 도라지를 급이하지 않은 군에 비하여 혈청과 간장의 총콜레스테롤과 중성지질의 농도가 현저하게 감소하였고, alloxan minihydrate를 체중 kg당 15 mg의 농도로 3주간 복강내 주사하여 당뇨를 유발시킨 흰쥐에 6년근과 22년근 도라지 추출물의 급이시 혈당 및 혈청과 간장의 지질 개선에 효과가 있었고, 또한 항암효과에 관한 연구로는 도라지 추출물을 B16-F1 mouse melanoma 세포에 처리해 laminin의 결합력을 실험한 결과 0.125 mg/ml 이상의 농도에서는 100%저해능을 보였으며, mice에 있어서 고형암의 무게를 61.5% 감소시켰으며 sacoma-180 ascites에 대한 생존일수 연장율이 128%에 달하는 것으로 관찰되었다. 도라지의 에탄올 추출물을 극성을 달리한 용매로 분획하여 각 획분의 항균성을 실험한 결과 23년근 도라지의 부탄올획분의 M. luteus에 대한 초소저해농도는 3년근에 비하여 28배나 더 낮은 값을 보였으며, 이를 실리카겔 칼럼 그로마트그래피와 HPLC를 이용하여 에틸아세테이트획분의 항균물질을 분리·정제한 결과 데틸아세테이트획분보다 S. epidermidis에 대해 2∼3배 더 강한 항균력이 관찰되었고 23년근 도라지의 주요 항균물질을 동정한 결과 giycerol linolate임이 확인되었다. The root of Platycodon grandiflorum (p.g.) A. DC (Platicodi radix) has been prescribed frequently for cough and bronchitis as an expectorant in a traditional oriental medicine, and used as a food. Diet-induced hyperlipidenic rats were fed diets containing 5% and 10% p.g (twenty-three years). powder for 3 weeks, the p.g. feeding markedly decreased both serum and liver lipid concentrationds in hyperlipidemic rats. Especially, 5% p.g. diet significantly decreased the concentrations of total cholesteril and triglycerides in serum and liver as compared with those of thr hyperlipidemic control group. The effects of the feeding p.g. (6 or 22 years) extract on the blood glucose, lipids in the serum and liver of alloxan induced hyperglycemic rats (S.D. strain, ♂), alloxan monohydrate 15㎎/㎏ B.W./day, I.P. injection for 3 weeks. concentrations of blood glucose were significantly higher in the allozan administration groups than those in the control group. When p.g. extracts treated in vitro with B16-F1 mouse melanoma cell lines, it showed 100% laminin-binding inhibitory activities at the concentration over 0.125 mg/ml in vivo test it showed 61.5% reduction of the solid tumor weight transplanted in mice and exhibited anticancer activity of 128% ILS against sarcoma-180 ascites. The ethanol extract of p.g. was fractionated by various solvents with different polarities, and the antibacterial activities of each fraction were tested. The minimal inhibitory concentration (MIC) values of each graction of Platycodi radix (twenty-three years) ethanol extract was lower than that of Platycodi radiz (three years) ehanol extract. the MIC value of Platycodi radix(twenty-three years) butanol fraction against M. luteus was 28 times lower than that of Platycodi radiz (three years) butanol fraction. The antibacterial compound was isolated and purified from the ethylacetate fraction by silica gel column chromatography and HPLC, and exhibited 2 to 3 times more antibacterial activity against S. epidermidis than ethylacetate fraction. The major antibacterial compound of Platycodi radix (twenty-three years) was identified to be a glycerol linolate.

일개 지역에서 b형 헤모필루스 인플루엔자 뇌수막염 예방접종을 하게 되는 경로

사은주,이동욱,성낙진,박기흠 東國大學校醫學硏究所 2007 東國醫學 Vol.14 No.1

We aimed to investigate people's recognition of immunization for Haemophilus influenza type b(Hib) meningitis, their knowledge of the vaccination as an option, the resources for parents, and the effective measures of promoting vaccination rate. From April 20th to May lOth, 2006, we assessed children who visited either a public health center or an university hospital for scheduled vaccination. We analyzed their general characteristics, vaccination rate for Hib, and resources for parents by using the self-written questionnaires. Furhermore, ingestigator rechecked the questionnaire by one-on-one interview. Among the collected self-written questionnaires, the adequately answered cases for the study were conducted by 95 parents from public health center group and 95 from university medical center group. 77 children (81.1%) received vaccination at public health center and 79 children (81.4%) at university medical center. 133 parents (69.3%) had the knowledge of Hib vaccination and as an optional injection, 52 parents (27.1%) knew about the vaccination but without the immunization awareness as an option, and 7 parents (3.6%) did not even know the Hib vaccination itself. Out of 156 children who underwent the vaccination, most parents of 87 (56%) obtained the resources on Hib vaccination from their baby check booklets. Despite the fact that the Hib immunization is an optional vaccine, almost all of people still seek for the Hib vaccination. The good use of baby check booklets would be a significant tool for immunization awareness. 5세이하 소아들의 보호자들이 b형 헤모필루스 인플루엔자 뇌수막염(이하 Hib) 예방접종에 관해 알고 있는지, 이 접종이 선별접종임을 알고 있는지, 또 어떤 경로로 접종하고 있는지를 조사하여 접종률을 올릴 수 있는 방안을 모색해 보고자 이 조사를 시행하였다. 이 연구는 2006년 4월 20일부터 5월 10일까지 서로 인접한 일개시의 보건소 및 대학병원에서 기본접종을 위해 내원한 15개월 이상의 소아들을 대상으로 조사하였다. 미리 구성한 자기기입식 설문지를 이용하여 일반적 특성, Hib 예방접종의 접종률 및 접종경로 등을 조사하였다. 이후에 조사자가 설문지 내용을 1:1 면담으로 다시 확인하였다. 그 결과 Hib 예방 접종을 시행한 경우는 보건소에서 조사한 95명 중 77명(81.1%),대학병원에서 조사한 97명 중 79명(81.4%)이었다. Hib 예방접종이 선별접종임을 아는 경우는 133명(69.3%)이었으며 Hib 예방접종은 알고 있지만 선별접종임을 모르는 경우가 52명(27.1%), Hib 예방접종 자체를 모르는 경우는 7명(3.6%)이었다. Hib 예방접종을 시행한 156명 중 Hib 예방접종을 알게 된 경위가 아기수첩을 보고 알게 된 사람이 87명(56%)으로 가장 많았다. Hib 예방접종이 선별접종임에도 불구하고 대다수에서 접종을 하고 있으며 아기수첩의 적절한 활용이 예방접종률을 높이는데 중요한 수단이 될 수 있다.

상호상관 함수를 이용한 지중선로 고장점 추정

朴星完,林泳範,鄭州桓,成洛煥,金喆煥,申明徹 成均館大學校 科學技術硏究所 1994 論文集 Vol.45 No.2

This paper is intended as an investigation of fault location for underground line. Over the past few decades a considerable number of studies have been made on protection of underground line. However, these approaches require the interruption of electric power supply from source for fault location estimation. Therefore, we proposed fault location algorithm for underground line protection without interruption of electric power supply. Cross correlation function is used in this algorithm. Also, EMTP data is used to demonstrate the capability of the proposed fault location algorithm.

천마분말이 고콜레스테롤혈증 흰쥐 혈청의 지질성분에 미치는 영향

박미연,성낙주,신정애,이수정,박필숙 동아시아식생활학회 1998 동아시아식생활학회지 Vol.8 No.1

This study was designed to investigate the optimum recommendation level on Gastrodia rhizoma and the effects on the improvement of the lipids in the dietary hypercholesterolemic rats. Experimental diets mixed with 5% Dioscorea batatas(Group 2), 10% Dioscorea batatas(Group 3), 15% Dioscorea batatas(Group 4), 5% Gastrodia rhizoma(Group 5), 10% Gastrodia rhizoma (Group 6) and 15% Gastrodia rhizoma(Group 7) were administered to the male rats of the Sprague Dawley for 3 weeks. Concentration of total cholesterol in serum was lower in the Gastrodia rhizoma groups than in the other groups, especially total cholesterol concentration of 10% Gastrodia rhizoma(Group 6) was the lowest in the Gastrodia rhizoma groups. Concentration of HDL-cholesterol in serum was higher in the 10% Gastrodia rhizoma and 15% Gastrodia rhizoma than in the other groups. Concentrations of cholesterylester, LDL, LDL-cholesterol in serum were the lowest in the 10% Gastrodia rhizoma. Concentration of glucose and activity of GPT in serum were the lowest in the 10% Gastrodia rhizoma group. The activity of GOT in serum was lower in the 10% Gastrodia rhizoma group and 15% Gastrodia rhizoma group than in the other groups. Therefore, we consider that there are effects on the improvement of the lipids in the Gastrodia rhizoma and the optimum recommendation quantity of Gastrodia rhizoma is 10% to quantity of food composition.

마분말과 천마분말이 흰쥐의 간, 뇌 및 신장의 지질성분 변화에 미치는 영향

박필숙,성낙주,박미연 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2

The present study tested to recommend optimum level of Gastrodia rhizoma and effect on the compositional changes of the lipids in Sprague Dawley rats which were fed control diet adding 5, 10, 15% Dioscorea batatas powder and 5, 10, 15% Gastrodia rbizoma powder for 3 weeks, respectively, The contents of moisture, ash, fat, protein and carbohydrate in dried Gastrodia rbizoma were 5.9, 2.7, 2.1, 0.9 and 88.4%(w/w) respectively. Concentrations of total cholesterol and free cholesterol in liver were the lowest in the 10% Gastrodia rbizoma group. Concentrations of triglyceride and phospholipid in liver were lower in the 10 and 15% Gastrodia rhizoma groups than in the other groups. Concentrations of total cholesterol, free cholesterol, triglyceride and phospholipid in brain were significantly lower in the group 6, 7 compared to the other groups. Concentrations of triglyceride and phospholipid in kidney were slightly lower in the Gastrodia rhizoma groups than those in the Dioscorea batatas groups.

항균성 동치미액의 첨가에 의한 냉면국물 중의 Listeria monocytogenes 및 Escherichia coli O157:H7 생육억제

소명환,박상희,조신호,황한준,성낙주 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.2

동치미액은 전통적으로 냉면국물로 이용되어왔다. 냉면국물의 미생물 오염문제를 해결하기 위하여 항균활성이 높은 김치 젖산균 Lactobacillus homohiochii B21과 Leuconostoc mesenteroides subsp. mesenteroides C16을 스타터로 혼합사용하여 동치미액을 제조하고, 이를 냉면국물에 100%, 50%, 10% 및 0% 첨가하여 20℃ 및 10℃에서 보관할 때에 동치미액의 항균력으로 인하여 의도적으로 첨가한 Listeria monocytogenes와 Escherichia coli O157:H7의 증식이 억제되는 정도를 조사하였다. 동치미액 100%인 냉면국물은 20℃에서 보관할 때에 Listeria monocytogenes는 8시간만에, Escherichia coli O157:H7는 40시간만이 10^6CFU/ml에서 10^0CFU/ml로 각각 급격히 사멸하였고, 10℃에서 보관할 때에도 시간경과에 따라 생균수가 급격히 감소하였으나 감소속도는 20℃에서 보관할 때보다 느렸다. 동치미액을 50% 첨가한 육수에서도 Listeria monocytogenes와 Escherichia coli O157:H7는 시간경과에 따라 급격히 감소하였으며, 감소속도는 동치미액 100%일 경우보다 느렸다. 동치미액을 10% 첨가한 육수는 20℃에서 보관할 때는 초기에 Listeria monocytogenes 및 Escherichia coli O157:H7의 균수증가가 약간 있었으나 동치미액 무첨가에 비하여 현저히 억제되었으며, 16시간 이후부터는 균수가 서서히 감소하였다. 동치미액을 첨가하지 않은 육수는 20℃에서 보관할 때는 초기부터 Listeria monocytogenes 및 Escherichia coli O157:H7의 균수증가가 급격히 이루어졌으며, 10℃에서 보관할 때는 24시간 이후부터 서서히 증가하였다. Listeria monocytogenes 및 Escherichia coli O157:H7에 대한 본 동치미액의 항균활성은 후자의 미생물보다 전자에 대하여 더욱 강하게 나타났다. 본 연구의 동치미액을 육수에 50% 첨가하여 냉면국물을 제조한다면 보관중의 미생물 오염문제 해결에 큰 도움이 될 것으로 판단된다. Juice of Dongchimi, a Korean traditional vegetable food fermented with lactic acid bacteria has been traditionary used as broth for Naengmyon, a Korean cold noodles with broth. This study was carried out to demonstrate the growth inhibition of two food born pathogens, Listeria monocytogenes and Escherichia coli O157:H7, in Naengmyon-broth containing Dongchimi-juice fermented with high antibacterial lactic acid bacteria, Lactobacillus homohiochii B21 and Leuconostoc mesenteroides C16. Naengmyon-broths were made with beef broth and Dongchimi-juice fermented with lactic acid bacteria, and the changes in viable cell counts of the inoculated pathogens in Naengmyon-broths were investigated during storage at 20℃ and 10℃. In Naengmyon-broth of 100% Dongchimi-juice stored at 20℃, the numbers of Listeria monocytogenes and Escherichia coli O157:H7 were rapidly decreased from 10^6CFU/ml to 10^0CFU/ml in 8 hours and 40 hours, respectively. In Naengmyon-broth containing 50% Dongchimi-juice, their numbers were also rapidly decreased, though the decreasing rates were not so fast as those in 100% Dongchimi-juice. In Naengmyon-broth containing 10% Dongchimi-juice, the growths of the two pathogens were markedly inhibited, compared with those in 100% beef broth, though some growths were occurred in early phase. But in Naengmyon-broth of 100% beef broth, their growths were very fast from early. Antibacterial activity of the Dongchimi-juice was more distinct at 20℃ than at 10℃, and was more active against Listeria monocytogenes than against Escherichia coli O157:H7.

꽃새우 乾製品의 맛成分 : 1. 유리아미노酸의 變化 1. Changes of Free Amino Acids in Trachypenaeus curvirostris During Sun Drying

李惠淑,金奉玉,成洛珠,鄭承鏞 진주여자전문대학 1981 論文集 Vol.3 No.-

Changes of free amino acids a s taste compounds during sun drying of shrimp Trachypenaeus curvirostris, were analyzed by amino acid autoanalyzer. Glycine, arginine, proline and alanine were dominant amino acids in fresh extracts, having 44.8%( 6109.9㎎%, on dry base), 21.9% (2989.3㎎%), 10.4% (1418.3㎎%)and 5.9% (805.1㎎%) of the total amino acids, respect-ively. Free amino acids were not changed in composition during sun drying of shrimp. Glycine, arginine, proline and alanine were dominant in both fresh extracts and dried products. In dried extracts,.such amino acids as glycine, arginine, proline and alanine were 44.7% (4162.3㎎%, on dry base), 24.1% (2244.61119 %), 11.7% (1090.l㎎%)and 5.3% (495.8㎎%) of the total amino acids, respectively. It is believed that glycicne, arginine, proline and alanine may play an important role as taste compounds in sun dried shrimp.

염건조기(굴비)의 가공조건이 n-Nitrosamine(NA)의 생성에 미치는 영향-1 보. 염건조기의 가공 · 저장 중 아민류, 질산염 및 아질산염의 변화- : 1보.염건조기의 가공·저장 중 아민류, 질산염 및 아질산염의 변화 1.Changes of Amines, Nitrate and Nitrite in the Salted and Dried Yellow Corvenla During Its Processing and Storage

이수정,신정혜,김정균,성낙주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

염건조기(굴비)는 참조기를 염장·건조하여 만든 염건품으로 예부터 우리나라 사라들이 즐겨 먹어 왔으며 오늘날까지 그 수요가 증가하고 있는 우리나라 특유의 수산가공품이다. 본 실험은 굴비를 가공하는 데 있어서 염의 종류, 염장방법을 달리하여 가공할 때 발암성 NA의 전구물질인 DMA, TMA, nitrate 및 nitrite를 분석함으로써 굴비의 안전성을 위한 기초자료를 확보코져 하였다. DMA 및 TMA 함량은 굴비 가공 및 저장중 증가하는 경향을 보였다. DMA는 생시료에서 0.3㎎/㎏였던 것이 염장 3일 후에는 17.4∼25.2배 증가하였고, TMA는 염건 15일후에 9.6∼15.4㎎/㎏의 범위였는데, 대체로 마른간법으로 제조한 시료에서 DMA 및 TMA의 증가폭이 더 큰 것으로 나타났다 질산염 및 아질산염함량은 생시료에서 각각 3.6, 0.4㎎/㎏으로 검출되었는데, 가공 및 저장중 점차적으로 감소하는 경향이었으나 질산염함량은 저장 40일 후에 5.2∼6.5㎎/㎏의 범위로 다소 증가되었다. DMA, TMA, 질산염 및 아질산염과 같은 NA전구물질의 생성을 억제시키는 측면에서 볼 때 정제염을 이용하여 물간법으로 염장하는 것이 비교적 효과적이라는 결과를 얻었다. The changes of amine, nitrate and nitrite nitrogen in yellow corvenia were studied during its processing and storage in order to clarify the precursors of N-nitrosamine(NA) formation in the salted and dried yellow corvenia(Gulbi), prpared by using the different salting method like dry and brine salting by pure and crude salt. As a result, during the processing and storage of Gulbi, DMA and TMA contents were significantly increased in the yellow corvenia. And after 40 days storage the increase rate showed 25.7∼45.7, 3.3∼5.6 times higher than those of 0.3, 2.4㎎/㎏, respectively, in raw sample. Regardless of the processing condition, contents of nitrate were also increased irregularlly, while nitrite contents, during its processing and storage, were scarcely changed in the salted and dried yellow corvenia. During the processing and storage of Gulbi, DMA and TMA contents were less produced in brine salted and dried yellow corvenia using crude salt than in sample prepared using pure salt, while the former were more effective than the latter in inhibiting the production of nitrate and nitrite. Therefore, it was revealed that reductions of NA precursors such as DMA, TA, nitrate and nitrite were more effective in preparing with the brine salting method than with the dry salting method.

멸치젓 숙성 중 N-nitrosamine의 생성

박희정,신정혜,강민정,성낙주 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.1

본 실험에서는 시판 젓갈류 중의 N-nitrosamine(NA)과 그 전구물질의 함량을 분석하였고, 식염 20%의 멸치젓을 제조하여 90일간 숙성시키면서 NA와 그 전구물질의 변화를 분석하였다. 시판 멸치젓(5점), 액젓(3점) 및 새우젓(2점) 중의 dimethylamine(DMA)은 32.7~361.1㎎/㎏, trimethylamine(TMA)은 흔적량~4.4㎎/㎏, 질산염과 아질산염은 각각 1.9~2.5㎎/㎏, 0.6~0.9㎎/㎏의 범위였다. NA는 N-nitrosodimethylamine(NDMA)만이 검출되었으며 1.9~10.1㎍/㎏의 범위였다. 멸치젓의 숙성 중 pH는 숙성 30일에 6.3으로 산성화되는 경향을 보이다가 그 이후부터는 거의 변화가 없었다. 또 염도는 숙성기간 동안 15.3~16.1%의 범위로 숙성기간에 따른 차이가 미미하였으며, 산도는 숙성 60일까지는 1.2%에서 1.4%로 다소간 증가하는 경향을 보이다가 그 이후부터는 거의 변화를 보잊 않았다. DMA는 숙성 30일까지는 완만한 증거를 보이다가 숙성 60일 후에 55.2㎎/㎏으로 급격히 증가하였고 TMA는 숙성 중 증가하다가 30일부터 현저히 감소하여 숙성 60일 이후에는 흔적량까지 감소하였다. 멸치젓 숙성 중 질산염은 생시료이 경우 1.5㎎/㎏였던 것이 숙성 60일 후에 4.3㎎/㎏으로 증가하였다가 점차 감소하는 경향을 보였으며, 아질산염은 생시료에서 0.6㎎/㎏이었으며 숙성 중 완만한 증가를 보였다. NA는 NDMA만 검출되었으며 생시료에서는 검출되지 않았으나 저장 20일 후에는 4.7㎍/㎏이었으며, 저장 90일까지 다소 증가하는 경향을 나타내었다. This study was attempted to analysis of N-nitrosamine(NA) and its precursors for elevating safety of fermented fish. Of the 10 samples marked, DMA, TMA, nitrate and nitrite contents were 32.7~361.1㎎/㎏, <0.1~4.4㎎/㎏, 1.9~2.5㎎/㎏ and 0.6~0.9㎎/㎏, respectively. NA was detected only N-nitrosodimethylamine(NDMA) and its levels were 1.9~10.1㎍/㎏. Anchovy sample after fermented for 90 days in our lab, dimethylamine(DMA) contents were slowly increased but, increased significantly to 55.2㎎/㎏ after 60 days of fermentation. Trimethylamine(TMA) contents were increased during fermentation and then drastically decreased after 30 days finally becoming a trace. Nitrate contents were transiently increased from 1.5㎎/㎏ in raw sample to 4.3㎎/㎏ after 60 days fermentation and then it is decreased gradually while nitrite content showed a slow increase rate from 0.6㎎/㎏ in raw sample during its fermentation. NA was not detected in raw sample but after 20 days of fermentation NDMA contents became 4.7㎍/㎏ and during 90 days fermentation it showed a slow increase rate.

영아자(Phyteuma japonicum Miq.)의 성분 조성

정미자,신정혜,이수정,홍성국,강호중,성낙주 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.4

산채식물인 영아자(Horned Rampion ; Phyteuma japonicum Miq.)의 영양학적인 가치를 평가코져 야생 및 평지재배 시료를 잎과 줄기로 구분하여 일반성분, 비타민 C, 유리당, 무기물, 핵산관련물질, 구성 아미노산 및 유리아미노산을 분석하였다. 야생과 재배 영아자의 회분은 1.2∼2.7%의 범위였고, 조지방과 조단백질은 재배시료가 조섬유는 야생시료에서 더 높은 함량으로 정량되었고, 전당은 두 시료간에 대차를 보이지 않았다. 비타민 C는 줄기보다는 잎에서 재배시료보다는 야생시료에서 더 높게 정량되었다. 유리당은 야생 및 재배시료 모두 glucose, frucose 및 sucrose가 잎보다 줄기에서 높게 정량되었다. 무기물은 총 9종이 분석되었는데 이중 칼슘의 함량이 가장 높아 재배시료의 경우 잎은 34374.0㎎/㎏, 즐기는 9584.1㎎/㎏였고, 그 다음으로 칼륨, 마그네슘의 순으로 많았으며, 야생시료도 비슷한 경향을 보였다. 핵산 관련물질은 CMP, UMP, IMP, AMP 및 hypoxanthine이 동정되었는데 잎과 줄기 모두 야생시료에서는 hypoxanthine이, 재배시료에서는 AMP가 월등히 높게 정량되었다. 구성아미노산은 총 17종으로 야생시료에서는 glutamic acid, 재배시료는 잎의 경우 aspartic acid, 줄기의 경우 glutamic acid의 함량이 가장 많았다. 유리아미노산은 총 29종이 동정되었고 야생시료의 잎에서는 glutamic acid, 줄기에서는 γ-aminoisobutyric acid, 재배시료의 줄기에서는 asparagine이 가장 높은 함량으로 정량되었다. This research is to establish the basic data of the nutritive value and improve our diet. In the part of th leaf and stem of the wild cultivated horned rampion(Phyteuma japonicum Mig), the components such as chemical composition, vitamin C, free sugar, mineral, nucleotide and its related compounds, composition and free amino acid were analyzed one after another. Content of the crude lipids and proteins was determined much higher in its wildness than in its cultivated horned rampion ; while, that of carbohydrates was higher in the former than in the latter. The content of vitamin C was retained higher in the leaf than in the stem horned rampion. And the content of calcium among the detected minerals was outstanding in all of the samples collected, and potassium and magnesium was the next ones in its order. The main components of free sugars in both the wild and cultivated horned rampion were glucose and fructose, and their content was higher in the stem than in the leaf. Nucleotide and its related compounds were identified with 5 kinds of nuciotides such as CMP, UMP, IMP, AMP and hypoxanthine(Hx), and the content of Hx and AMP was the highest in the wild and cultivated samples, respcetively. In the composition amino acid of the wild horned rampion, glutamic acid, aspartic acid and phenylalanine was outstandingly abundant; while, such amino acid as methionine and proline was small and besides cysteine couldn't be detected in the stem. Total amounts of composition amino acid in the leaf was 2118.0 and 1120.1㎎% in the wild and cultivated sample, respectively. In the free amino acid of horned rampion, the total amount ranged from 8.5 to 50.1㎎%, which were lower level than that of composition amino acid. But the number of free amino acid was 29 kinds, which was bigger in its number than of composition amino acid detected 17 kinds.