Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

코로나 플라즈마 처리에 의한 HDPE 표면의 개질에 관한 연구

최진숙,전종탁,정봉규,이근대,문명준,이종무 釜慶大學校 2001 釜慶大學校 論文集 Vol.6 No.-

We are able to generate the stable plasma in open air and nitrogen using a corona plasma treatment system(CPTS) with a high voltage. This was applied to treat the surface of rather non-polar HDPE(High Density Polyethylene) intended for the improvement of adhesion of over-coated layers such as coatings, printing inks. The surface tension of HDPE was significantly improved by the CPTS. The characteristics of surface layer were evaluated by means of contact angle measurement, ATR-FT/IR spectroscopy, and it was found that functional groups were introduced into the surface layer of the HDPE. The use of nitrogen was increased the surface tension of the HDPE from 33.8 mJ/m^2 to 66.56 mJ/m^2, and highly effective method of HDPE surface modification could be realized.

보건계열 중 간호과와 안경광학과 학생들의 학습 성향 분석

최계훈,소명숙 대구보건전문대학교 2006 대구보건대학 論文集 Vol.26 No.-

The lst grade of Nur (Department of Nursing), ACT was higher than REF, but ACT was lower than REF in the 2nd and 3rd grade. SEN was increased in the 2nd grade more than lst grade, but it was decreased in the 3rd grade, VIS as well. The case of SEQ, it developed a tendency to increase as their grade was higher. The Oph (Department of Ophthalmic Optics) showed a tendency to REF, but it changed to propensity of ACT in the 2nd grade. And also propensity of SEN was much higher than INT when they were in the lst grade, but the gap was reduced when they were 2nd grade. As the grade was higher, the students had preference of VIS over VRB and the gap of the preference between VIS and VRB was increased, the propensity of SEQ was increased as well. Therefore the first students in the Department of Nursing showed strong propensity of ACT and they changed to the propensity of REF after. The students of the Department of Ophthalmic Optics showed the opposite propensity. The other learning propensity showed that the propensity of the first grade maintained to upper grade.

대두유 첨가 냉동저장 녹두전분겔의 품질특성

최은정, 오명숙 가톨릭대학교 생활과학연구소 2011 생활과학연구논집 Vol.31 No.1

악성 질환에서의 고용량 화학요법과 자가말초혈액 조혈모세포이식술 : 단일기관 치료 경험

최정혜,안명주,오호석,이웅수,오석중,이영열,최일영,김인순 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.2

연구배경: 고용량 화학요법은 항암제의 투여 용량에 따라 항암효과가 비례하는 용량반응 관계가 있는 악성종양에서 효과를 기대할 수 있으며 현재 급성백혈병, 악성림프종, 다발성골수종 등의 혈액 종양과 유방암, 고환암, 난소암, 신경아세포종 등의 고형암 환자에서 시행되고 있다. 저자들은 고용량화학요법에 이어 자가조혈모세포이식을 시행 받은 악성 종양 환자를 대상으로 치료효과 및 부작용 등의 임상상을 알아보고자 하였다. 방법: 1997년부터 2003년까지 한양대학교 병원에서 자가조혈모세포이식을 시행 받은 악성 종양 환자 31예(남서 : 여성 15 : 16, 중앙연령: 50세, 다발성골수종 14예, 비호지킨림프종 9예, 유방암 4예, 호지킨림프종 3예, 급성골수성백혈병 1예)를 대상으로 후향적 분석을 시행하였다. 결과: 채집된 단핵구의 중앙값은 5.1×10^(8)/kg (범위 1.6~189.3)이었고 CD34양성세포수의 중앙값은 12.0×10^(6)/kg (범위 1.3~75.1)이었다. 말초혈액 과립구가 500/μL 에 도달하는 중앙값은 11일(범위 8~27)이었고 혈소판이 20,000/μL에 도달하는 중앙값은 11일(범위 0~32)이었다. 고용량 항암화학요법 후 21예가 완전관해, 5예가 부분관해에 도달하였다. 생존한 환자의 중앙추적기간은 29.5개월이었고 전체 환자의 2년 무병생존기간은 55.7%이었고 중앙값은 48.2개월이었다. 2년 생존기간은 62.9%이었으며 중앙값에는 도달하지 않았다. 10예의 사망환자 중 7예가 질병의 진행으로 사망하였고, 조기사망 3예(심부전과 신부전 1예, 장파열 1예, 패혈증 1예)가 있었다. 결론: 이 연구는 포함된 환자의 수가 적고 추적기간이 짧은 단점이 있으나, 다발성골수종, 악성림프종 등의 악성 질환에서 고용량 항암화학요법과 자가조혈모세포이식은 안전하고 효과적인 치료임을 추정할 수 있었다. Background: We performed the current study to evaluate the safety and efficacy of high-dose chemotherapy (HDC) with autologous peripheral blood stem cell transplantation (ASCT) in malignant diseases. Methods: Between January 1997 and March 2003, 31 patients (14 multiple myeloma, 9 Non-Hodgkin's lymphoma, 3 Hodgkin's disease, 4 breast cancer, and 1 acute myelogenous leukemia) underwent HDC with ASCT. Stem cells were collected by CS-3000 after mobilization treatment with chemotherapy and granulocyte colony-stimulating factor. The conditioning regimens included high-dose melphalan, BEAM (BCNU, etoposide, cytosine arabinoside, melphalan), BEAC (BCNU, etoposide, cytosine arabinoside, cyclophosphamide), CPB (cyclophosphamide, cisplatin, BCNU) and others. Results: There were 15 male and 16 female patients and median age was 50 years (range: 29~77). The median number of mononuclear and CD34+ cells was 5.1×10^(8)/kg (1.6~189.3) and 12.0×10^(6)/kg (1.3~75.1), respectively. The median time of neutrophil (>500/μL) and platelet (>20,000/μL) engraftment was 11 (8~27) and 11 (0~32) days after ASCT, respectively. Twenty-two patients experienced neutropenic fever. Twenty-one patients achieved complete remission and 5 patients achieved partial remission after HDC with ASCT. The median follow-up duration for surviving patients was 29.5 months. Causes of death included disease progression (7), sepsis (1), bowel perforation (1), and renal failure (1). Median progression free survival (PFS) was 48.2 months and median overall survival (OS) was not reached. Two-year PFS and OS was 55.7% and 62.9%, respectively. Conclusion: Our results suggest that HDC with ASCT was safe and effective modality in patients with various malignant diseases.

산마늘로부터 단리한 kaempferol과 quercetin의 콜레스테롤 저하 활성

이성숙,문서현,이학주,최돈하,조명행 한국목재공학회 2004 목재공학 Vol.32 No.1

식용 임산자원인 산채류를 기능성 식품으로 개발하고자 산마늘을 비롯한 충 13종의 에탄을 조추출물에 대한 콜레스테롤 저하활성을 검정하였다. 즉, 콜레스테롤 생합성 과정 초기에 관여하는 3-hydroxy-3-methylglutaryl coenzyme A reductase(HMG-CoA reductase)와 후기에 관여하는 squalene synthase의 효소 활성을 조사한 결과 산마늘 잎 에탄올 추출물이 두 효소의 활성을 공히 70% 이상 저해하여 활성이 가장 우수한 것으로 나타났다. 그리고 이러한 콜레스테롤 저하활성과 관련이 있는 물질을 탐색하고자 산마늘로부터 물질 단리를 시도하여 디클로로메탄 가용부로부터 kaempferol과 quercetin을 단리하였다. 또한 유전자 레벨에서의 콜레스테롤 저하 활성을 조사하기 위해 단리물질과 분획물을 C100세포(햄스터 유래 HMG-CoA reductase 고발현 세포주)에 각각 5㎍/㎖과 10㎍/㎖로 24시간 처리하여 HMG-CoA reductase와 squalene synthase의 mRNA 발현 정도를 조사하였다. 그 결과 10㎍/㎖로 kaempferol과 quercetin을 처리한 경우 두 효소의 mRNA가 전혀 발현하지 않는 것으로 나타나 유전자 레벨에서의 콜레스테롤 생합성 저해 효과를 확인할 수 있었다. 이상의 결과 산마늘 잎 에탄올 추출물은 콜레스테롤 생합성에 관여하는 HMG-CoA reductase와 squalene synthase의 활성을 저해하며 이러한 활성 저해 효과는 kaempferol과 quercetin에 기인하는 것으로 사료되었다. 특히 kaempferol과 auercetin은 여러식물의 성분으로서 이미 알려진 화합물이지만 콜레스테롤 저하활성이 있는 것으로 밝혀진 것은 이번이 처음으로 금후 이들 물질과 이들 물질을 함유하고 있는 식물 활용에 필요한 자료를 제공하였다고 사료된다.

초고속 통신망에서 비디오의 주관적 품질 평가에 관한 연구

강명화,강진석,최연성,김장형 濟州大學校 産業技術硏究所 2001 산업기술연구소논문집 Vol.12 No.1

According to information communication business faces with competitions in a various spheres with the development of multimedia and information communication. the time when we have to make an effort to improve a quality comes from an era when we devote ourselves to quantitative expansion. we will try to perform quality assessments of real-time interactive multimedia service. and so on - in this research and present criteria of new subjective quality assessments which is fit on Korea by analyzing examples of ITU-T and foreign countries and Korea.

측정값의 상황 의존성

김명석,최상돈,조상규,김정호,전헌무,김호일 慶北大學校 師範大學 科學敎育硏究所 1998 科學敎育硏究誌 Vol.22 No.-

We review the contextuality of measured values in quantum mechanics from point of view of a conventional interpretation of quantum mechanics. We will, in particular, concentrate on Bell-KS theorem which, seemingly, disproved the existence of a hidden-variable interpretation of quantum mechanics. We introduce ways to accommodate the contextuality so that Bell-Ks theorem no longer holds and therefore the existence of a hidden-variable interpretation becomes possible.

유산양에서 발정동기화 유도와 인공수정 효율

장석민,강재구,최기명,김홍래,진동일 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

This study was conducted to evaluate the response of CIDR-S on estrus synchronization of dairy goats and the effect of artificial insemination on conception rate. Estrus synchronization of sexually matured Saanen goats was induced with insertion of CIDR-S into vagina for 15 days and injection of 500 IU PMSG before removal of CIDR-S. Artificial insemination was conducted using liquid or frozen semen by intra-cervical insemination with synchronized does. Estrus synchronization was 90% using CIDR-S insertion for 15 days. Conception rate of inseminated does was 20% - 25%, while that of natural mated does was 100%. These results suggested that synchronization of dairy goats can be successfully induced and artificial insemination method has to be improved for the practical use in dairy goats.

Electron Cyclotron Resonance O_2 Plasma에서 증착한 규소 산화 박막의 특성

안명환,서문석,장재선,서성모,이기방,윤창주,이형재,남기석,최규현,손춘배,김용섭,강석희 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.17 No.-

규소 산화막을 ECR-CVD(electron cyclotron resonance-chemical vapor deposition) 증착방법으로 5인치 기판위에 상온에서 증착하고, 증착공정조건인 증착율, 기판온도, 마이크로파의 세기변화 및 플라즈마 혼합기체의 비에 따른 규소 산화막의 특성을 조사하였다. 또한 산화막의 구조적인 특성을 비교하기 위해 FTIR을 이용하여 ECR-CVD 증착한 산화막, RPE-CVD(remote plasma enhanced-CVD) 증착한 산화막 및 열 산화막의 stretching frequency를 측정하였다. 측정된 결과 ECR-CVD로 증착된 산화막이 구조적인 면에서 열 산화막과 거의 같음을 보였다. ECR-CVD로 증착된 산화막의 전기적인 특성을 전류-전압 및 축전-전압 측정에의하여 분석하였다. 축전된 산화막의 전기적인 특성은 산화막의 전하 밀도는 1×10 exp (11)/㎠이였고, 평균 절연 파괴 전압은 약 6 MV/㎝이다. We have grown thin films of SiO_2 at room temperature by using an ECR-CVD system and have investigated the changes in the properties of the deposited films with changes in the processing conditions such as the deposition rate, the substrate temperature, the microwave power and the plasma gas mixing ratio. We also measured the stretching frequency of three kinds of oxides, and ECR-CVD-grown oxide, a PECVD-grown oxide, and a thermally grown oxide, using FTIR analysis to compare their structural properties. The result shows that the structural properties of the ECR-grown oxide are similar to those of the thermally grown oxide. Additionally, the electrical properties of the ECR-grown oxide were investigated by using current-voltage and capacitance-voltage measurements. These electrical results indicate that the oxide charge density and the average breakdown voltage are 1×10 exp (11) ㎝^-2 and 6 MV/㎝, respectively.