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Metabolic shift of electrochemically active strain, P. putida2523 in microbial fuel cell
( Mutyala Sakuntala ),김창만,김중래 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
Pseudomonas putida is also known as electro-active in a microbial fuel cell system to produce electricity. Thus this strain is an ideal host for metabolic engineering for platform chemical production using electro based metabolic regulation. In this study, we have examined the metabolic shift of P. putida in different culture condition such as conventional fermentation and electrode respiring environment. The cell growth of P. putida was facilitated by electrode with simultaneous electricity generation and changes of byproduct formation. This strain during electro-fermentation discharged current up to 0.5mA/cm2 anodic current density and few metabolites acetate, lactate. These results implicate that metabolically engineered P. putida can provide a platform as a host cell for BES based biorefinery process with further metabolic engineering and optimization.
Electrode based redox maintenance and acetate oxidation of P.putida 2523 in a microbial fuel cell
( Mutyala Sakuntala ),( Changman Kim ),( Young Eun Song ),( Jiyun Beak ),( Minsoo Kim ),( Eunhee Seol ),김중래 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Microbial fuel cells have been recently highlighted for bioenergy and biochemicals production using various electrochemically active strains. Electrode in MFCs acts as source or sink for a respiratory electron of metabolic pathway, thus maintains intracellular redox ratio and viability as well as produces bioelectricity. Pseudomonas putida is also known as an electrochemically active bacteria, hence its metabolic capability can be regulated by electrode. In this study, P.putida 2523 strain is cultured using acetate as a sole carbon source in MFC anaerobically where the carbon electrode act as terminal electron acceptor. The acetate consumption and cell viability were compared with an open circuit MFC (i.e. electrode does not act as electron acceptor). In the presence of electrode NAD+ regeneration rate increased under MFC in anaerobic condition.
Mutyala, Anil Kumar,Park, Jong S. Elsevier 2016 Tetrahedron letters: the international organ for t Vol.57 No.10
<P><B>Abstract</B></P> <P>Unsymmetrical trisulfonated zinc phthalocyanine with single carboxylic acid (trisulfo-Pc-COOH) was newly prepared via the hydrolysis of cyanotrisulfo-Pc which was obtained from the palladium catalyzed cyanation of iodotrisulfo-Pc. The product exhibited good water solubility along with excellent absorption and emission characteristics in an aqueous condition, proving its feasibility for use in bio-imaging applications.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Sridevi, Mutyala,B.R., Arun Kumar International Journal of Computer ScienceNetwork S 2021 International journal of computer science and netw Vol.21 No.8
Alzheimer's is a chronic progressive disease which exhibits varied symptoms and behavioural traits from person to person. The deterioration in cognitive abilities is more noticeable through their Activities and Instrumental Activities of Daily Living rather than biological markers. This information discussed in social media communities was collected and features were extracted by using the proposed fuzzy logic based algorithm to address the uncertainties and imprecision in the data reported. The data thus obtained is used to train machine learning models in order to predict the longevity of the patients. Models built on features extracted using the proposed algorithm performs better than models trained on full set of features. Important findings are discussed and Support Vector Regressor with RBF kernel is identified as the best performing model in predicting the longevity of Alzheimer's patients. The results would prove to be of high value for healthcare practitioners and palliative care providers to design interventions that can alleviate the trauma faced by patients and caregivers due to chronic diseases.
Himanshu KHANDELWAL,Sakuntala MUTYALA,Jung Rae KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
The bacterial redox sensor has been highlighted since the monitoring and control of cellular redox state influence the cell metabolism and target metabolite production. In Pseudomonas putida, fpr ferrodoxin NADP+ reductase gene senses the ROS/oxidative stress and gives transcriptional response to defend against toxic compounds. Fpr regulation is known to be induced by the LysrR type transcriptional factor FinR. Such transcriptional regulation is helpful for bacteria to sense the oxidative stress resulting from the environmental factors induced by toxicity and/or or environmental pollutants. This mechanism could be applied to upregulate gene of interest placed downstream of the fpr gene. In this study, a dual plasmid was made to harbour finr-fpr gene and LacZ as reporter protein into the Pseudomonas sp. The redox stress/ ROS generated by various redox mediator like pyocyanin, Paraquat, HNQ and HQ and was carried out under anaerobic and aerobic conditions. The redox sensor was activated by use redox cycling drugs and generated response was measured. The β-galactosidase activity was quantified, and protein expression levels were analyzed. In further studies this property will be applied for induction of the gene of interest using the redox mediated stress in a Bio-electrochemical system (BES), which can be used for controlled modulation of the downstream gene expression by applied potential for higher production of the biofuels or valuable chemical synthesis. This result indicated that monitoring and controlling of bacterial redox stress can be used as a parameter to regulate gene expression and metabolite production. (Poster)