만성 신부전을 동반한 Laurence Moon-Bardet Biedl 증후군 1례

박래경,이동환,문철,김은미,Park Lae Kyong,Lee Dong Hwan,Moon Chul,Kim Eun Mi 대한소아신장학회 1998 Childhood kidney diseases Vol.2 No.2

The Laurence Moon-Bardet Biedl syndrome is characterized by obesity, mental retardation, visual impairment with retinitis pigmentosa, polydactyly, hypogonadism and renal manifestations. We experienced an 11 years old female with Laurence Moon-Baret Biedl syndrome associated chronic renal failure. She was diagnosed to have LMB syndrom according to the clinical manifestations of polydactyly on hands and feet, mental retardation, obesity, retinitis pigmentosa and chronic renal failure. She is on maintenance hemodialysis now.

Cationic Polyliposome-Mediated p53 Gene Therapy in Liver Cancer : Results of Experimental Study and Pilot Clinical Study

Moon, Woo-Chul,Lim, Hyun-Mook,Shim, Hyung-Jin,Moon, Chul-So 가톨릭의과학연구원 2000 가톨릭 의과학연구원 국제학술대회 Vol.4 No.-

p53 is a prototype tumor suppressor gene and plays a key role in regulation of cell cycle progression and apoptosis and maintenance of genomic integrity. We have carried out long-term studies to investigate antitumor efficacy and safety of cationic liposome-mediated p53 gene therapy in liver cancer. We constructed a complex of plasmid carrying cDNA of human wild type(WT) p53 and novel cationic polyliposome, DDL(in weight ratio of 1:3.375), and named it hepatovection-1(HV1). HV1 was produced in sterile and endotoxin-free status and its quality was controlled depending on the United States FDA guideline for human gene therapy using cationic liposome and plasmid DNA.

Optimality Theory In Syntax

(Moon Seung Chul) 대한언어학회 1997 언어학 Vol.5 No.1

Moon, Seung-Chul. 1997. Optimality Theory in Syntax. Linguistics, 5-1, 235-253. The purpose of this paper is to show that Optimality Theory developed in phonology can also be applied to syntax. I discuss possibilities of some syntactic phenomena which may be explained by OT. And I focus on the binding facts of the unbounded reflexives and show that seemingly unbounded binding relation can be explained by the interaction of universal constraints, under Optimality Theory as proposed in Prince and Smolensky (1993). For syntactic binding, I follow Moon (1995) and assume a series of constraints, THC, LPC, SOC, CCC, DBC which affect binding by their mutual interaction with each other. I conclude this paper by showing that the five constraints on anaphoric binding interact to produce the best choice of antecedents. (Hankuk Aviation University)

Cationic Liposome-Mediated p53 Gene Therapy in Liver Cancers: Result of Basic and Clinical Study

Moon,Young Ho,Kim,Byung Koo,Shim,Hyung Jin,Uhm,Tae Han,Cha,Sung Jae,Moon,Chul So,Moon,Woo-Chul,Kwak,Byung Kook,Gi,Young Jin,Lim,Hyun Mook,Yoo,Nam Sook,Lee,Chang Joon 가톨릭중앙의료원 가톨릭암센터 1998 암심포지움 Vol.- No.2

p53 is a prototype tumor suppressor gene, which plays key roles in regulation of cell cycle progression, apoptosis, DNA repair and maintenance of genomic stability. Experimental stydy study on p53 gene therapy for cancer have been widely tried and Success of adenovirus or retrovirus-mediated p53 gene therapy have been reported in patients with lung cancer and cancer of the head and neck. However, viral vector-mediated gene therapy may have demerits to be widely applied in human cancers. We herein have designed a cationic liposome-mediated p53 gene therapy and have studied its antitumor efficacy and safety in vitro, in vivo and in human patients with advanced liver cancers. We constructed and prepared a complex of plamid vector carrying cDNA of human wild type(WT) p53, cationic liposome (HECH : DOPE) and cationic polymer (polylysine) in sterile and endotoxin-free condition depending on the guideline of FDA and used this complex for in vitro and in vivo transfer oi WT p53 gene. In vitro administration of p53 gene to a variety of human cancer cells with point mutation or deletion of p53 in DNA dose of 1㎍ per ?? cells induced high level expression of WT p53 and p21/WAF1 as demonstrated by RT-PCR-Southern blot and Western blot, apoptosis as demonstrated by DNA electrophoresis, and significant decrease of viable cell numbers by 70.4 to 98.5%, whereas, administration of p53 gene in same condition to human cancer cells with WT p53 didn't induce either apoptosis or significant decrease of viable cell numbers. In vivo antitumor efficacy of p53 gene transfer was initially analyzed in sc,ip, subrenal capsular and intrahepatic xenograft model of human cancers with deletion of p53. Local, ip, or iv administration of p53 gene in DNA dose of 100㎍ daily for 5 to 10 days induced expression of WT p53 and p21 in tumor tissues, significant decrease of tumor volume (by 62.0 to 99.5%)and/or incidence of tumors, and complete suppression of metastasis. Intravenous administration of p53 gene in DNA dose of up to 12 mg/kg to rats didn't induce significant acute or sub-acute toxicity as demonstrated by survival body weight, laboratory studies, and histologic analysis of vital organs. Trans-hepatic arterial administration of p53 in DNA dose of 0.5 to 5 mg per kg induced expression of human WT p53 in rabbits liver and/or lung and didn't induce significant hepatic toxicity or systemic toxicity. Trans-hepatic arterial administration of p53 gene in DNA dose of 1 mg per kg induced significant decrease of tumor incidence (to 40%) and volume (to 0.4% of control) and suppression of metastasis of rabbits liver VX2 carcinoma which carry point mutation of p53. These results suggested that our cationic polyliposome-mediated p53 gene trasfer might be a promising treatment modality for human cancers which carry mutation of p53. Based on the results of basic research, we carried out a pilot study to investigate antitumor efficacy and safety of cationic liposome-mediated p53 gene therapy in patients with liver cancer. Patients whth advanced, primary (N=12) or metastatic (N=4) liver cancer who had failed conventional therapy underwent p53 gene therapy, of whom 12 showed mutation of p53. Patients with advanced liver cirrhosis or metastasis were also included in the study. Fifteen patients with advanced liver cancer who underwent hepatic angiography alone served as control. p53 gene was administered in complex with HECH : DOPE : PLL into common hepatic artery in DNA dose of 10 to 30 mg every 3 to 4 weeks for 2 to 8 times (mean 4 times). Of 16 patients who underwent p53 gene therapy, 6 showed partial response, 4 minimal response and 3 stable disease. High level expression of WT p53 or p21 were found in tumor tissues of hepatovectin1 administered patients. After p53 gene therapy, 11 of 16 patients developed transient fever and chill, but none developed transient fever and chill, but none developed grade 4 toxicity or deterioration of liver function. Of 16 patients who underwent p53 gene therapy, 7 were alive on 1 year follow up and 9 died from metastatic cancer or liver cirrhosis-related complications. in contrast, none of control group survived for 1 year. These results suggest that cationic liposome-mediated p53 gene trasfer via hepatic artery may become a promising therapeutic modality for advanced liver cancer which carry mutation of p53 gene. Our studies strongly suggest that our cationic liposome-mediated p53 gene therapy may be a promising treatment modality for human cancers which carry mutation of p53 gene and failed by conventional therapies. Further stydies are necessary on the actual benefit of this experimental study and effective systemic delivery method of p53 gene.

알루미늄을 투여한 흰쥐의 해마와 대뇌피질에서 Reactive Oxygen Species 생성으로 인한 생체거대분자의 산화적 손상

문철진(Chul-Jin Moon),고현철(Hyun-Chul Koh),신인철(In-Chul Shin),이은희(Eun-Hee Lee),문해란(Hae-Ran Moon) 한국독성학회 2004 한국독성학회지 Vol.20 No.3

This work aimed to study the effectiveness of cellular oxidative parameter (malondialdehyde,<br/> protein carbonyl, and 8-hydroxy-2'deoxyguanosine). The experimental groups were aluminum<br/> treated rats and control rats. Aluminum treatd rats were given intraperitoneally aluminum nitrate<br/> nonahydrate (Al3+, 0.2 mmol/kg) daily for 30 days except Sunday. Control rats were injected 1 ml of<br/> saline. After the dose, rats were decapitated and hippocampus and cerebral cortex were removed.<br/> The measured parameters were tissue malondialdehyde (MDA, index of lipid peroxidation), protein<br/> carbonyl (index of protein oxidation), 8-hydroxy-2'-deoxy-guanosine (8-OHdG, index of DNA oxidation),<br/> reduced glutathione (GSH) levels as well as glutathione reductase (GR) and catalase. Al concentrations<br/> in the tissues were also measured. All results were corrected by tissue protein levels. The<br/> results were as followed; 1. The concentrations of Al in the cortex and hippocampus were significantly<br/> higher in the Al-treated rats than in the control rats. 2. Antioxidative enzyme's activity, catalase<br/> and GR, were significantly higher in the Al-treated rats than the control rats. GSH levels were also<br/> higher in the Al-treated rats. 3. MDA, protein carbonyl, and 8-OHdG concentration of Al-treated rats<br/> were significantly higher than those of control rats. 4. The concentrations of antioxidants, and oxidative<br/> stress parameter were correlated with the concentrations of Al in hippocampus and cerebral cortex.<br/> Catalase and GR activity were also correlated with the concentration of Al. Based on these<br/> results, it can be suggested that intraperitoneally injected Al was accumulated in the brain and<br/> induced the increase of antioxidant levels and antioxidatve enzyme activity. Also, the oxidative products<br/> of cellular macromolecules are significantly related to tissue Al concentration. Therefore MDA,<br/> protein carbonyl, and 8-OHdG are useful markers for oxidative stress on cellular macromolecules.

Guidelines for the Development of Ethical Questions in the National Physical Therapy Examination

So-Ra Moon(So-Ra Moon),Myung-Chul Kim(Myung-Chul Kim) 한국보건의료윤리학회 2022 한국보건의료윤리학회지 Vol.1 No.1

Objective: We aim to be of help with developing ethical questions in the national physical therapy examination by presenting guidelines for its development. Methods: The code of ethics and values were identified through questionnaires, the types of questions without controversy over answers were presented with the answers through the ethics seminar, and the differences and ranges between ethics and medical technicians act were determined through a public hearing with experts. Results: A variety of opinions were collected for guidelines of ethical question development through several study methods, and as the result, the ethical questions were divided into a total of 4 types, including the types of being aware of the basic ethical theories and understanding of the concept of ethics through the conclusion that ethical theories and cases are all important, and the types of finding the most correct ethical items through the cases that physiotherapists can experience in the actual clinical field. Then, conclusions were obtained that questions of finding the most appropriate ethical attitudes by reading and interpreting sentences are needed but understanding of the concept and meaning about the ethical items is also important. Based upon this, we present guidelines for ethical question development of a total of 4 types in the forms of descriptive and short-answer questions. Conclusion: It is thought that this study will provide a foundation for ethical question development guidelines in the national physical therapy examination, and then play an important role of suggesting directions for ethics education in college.

Degranulation and Cytokine Expression in Human Cord Blood-derived Mast Cells Cultured in Serum-free Medium with Recombinant Human Stem Cell Factor

Hyeun Wook Chang,Tae Chul Moon,Eunkyung Lee,백석환,Makoto Murakami,Ichiro Kudo,Nung Soo Kim,Jong Myung Lee,Hae-Ki Min,Naotomo Kambe 한국분자세포생물학회 2003 Molecules and cells Vol.16 No.2

Composite underlayer/CoFeB/MgO structures with interfacial perpendicular magnetic anisotropy

Chul-Hyun Moon,Byoung-Chul Min,Kyung-Jin Lee,Kyung-Ho Shin 한국자기학회 2014 한국자기학회 학술연구발표회 논문개요집 Vol.2014 No.5

Impact of Corporate Cultural Diversity on International Strategy

Moon, Chul Woo Institute for International Trade and Cooperation 2007 Asian International Studies Review Vol.8 No.2

This paper addresses the issue of how three cultural factors i.e. cultural distance between countries, focal firm's prior experience in host country culture, and firm-level cultural diversity affect firm's choice of international strategic combination mode between M&A and joint ventures. Rooted in organizational learning approach to internationalization, our model specifies firm-level cultural diversity as a key determinant of mode choice. Empirical support was found in a sample of U.S. firms.

Continuous and Accurate PCRAM Current-voltage Model

Chul-Moon Jung,Eun-Sub Lee,Kyeong-Sik Min 대한전자공학회 2011 Journal of semiconductor technology and science Vol.11 No.3

In this paper, we propose a new Verilog-A current-voltage model for multi-level-cell PCRAMs. This model can describe the PCRAM operation not only in full SET and RESET states but also in the partial resistance states. And, 3 PCRAM operating regions of SET-RESET, Negative Differential Resistance, and strong-ON are unified into one equation in this model thereby any discontinuity that may introduce a convergence problem cannot be found in the new PCRAM model. The percentage error between the measured data and this model is as small as 7.4% on average compared to 60.1 % of the previous piecewise model. The parameter extraction which is embedded in the Verilog-A code can be done automatically.