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Control of major infectious bovine diseases in Korea
Moo Hyung Jun(金茂炯) 한국예방수의학회 1990 예방수의학회지 Vol.14 No.3
國民所得의 증가와 食生活 양식의 변화로 國民-人當 畜産物의 消費量이 매년 증가하고 있으며 이로 인해 외국으로부터 畜産物의 輸入量도 늘어나고 있는 실정이다. 국내 畜牛産業을 반전시키고 위생적인 畜産食品의 原料生産이란 측면에서 소의 傳染性 疾病 防疫은 매우 중요하다. 1900년 이래 한국에서는 약 20종류의 소 傳染性 疾病이 보고되었다. 이들 중 口歸疫, 牛疫 및 牛肺疫은 과거 50년간 발생되지 않아 완전히 根絶된 것으로 간주하고 있다. 그리고 急性熱性 傳染病인 炭疽와 氣腫疽는 集團豫防注射 실시를 위시한 여러 防疫對策에 의해 발생이 근절되어 가고 있으며, 또한 人獸共通傳染病으로 중요시되고 있는 牛結核과 부루셀라병은 診斷 및 陶汰에 의해 그 발생이 급격히 감소되고 있다. 소 傳染病의 防疫은 돼지나 닭에 비교해 볼 때 비교적 성공적이라고 평가되고 있으며, 이는 정부 당국과 각 분야에 근무하는 獸醫師 및 養畜家들이 유기적으로 협력하여 추진한 결과로 사료된다. 韓國에서 畜牛産業에 막대한 經濟的 손실을 야기할 뿐만 아니라 公衆保健學的으로도 문제되는 소의 傳染病을 防疫하기 위한 다음 4가지 사항에 대해 더욱 중점적으로 추진해야 한다. ⅰ) 慢性消耗性 疾患과 특히 최근에 문제되고 있는 아까바네병, 이바라기병 및 소유행열에 대한 豫防藥의 改良과 診斷法의 開發, ⅱ) 소 疾病ㆍ診療技術의 改善을 위해 分子生物學, 遣傳工學등과 같은 尖端科學技術을 응용해 나가야 한다. ⅲ) 疾病監視 및 豫察制度의 적극적 운용, ⅳ) 外來性 傳染病의 국내 流入을 방지하기 위해 檢疫制度의 강화
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Jun, Moo Hyung,Kim, Kyo Joon,Kim, Duck Hwan,Lee, Hun Jun 충남대학교 농업과학연구소 1988 農業技術硏究報告 Vol.15 No.-
To develop more specific and sensitive diagnostic methods for infectious bovine rhinotracheitis, 7-C-2 monoclonal antibody specific to polypeptides of infectious bovine rhinotracheitis virus(IBRV) was applied in indirect immunofluorescence antibody assay (IFA), indirect immunoperoxidase assay (IPA) and radial immunodiffusion enzyme assay (RIDEA). It was found that IBRV infected in MDBK cells could be detected as early as 8 hrs post infection by IFA. and that IFA was more rapid and specific to identify IBRV antigen than IPA. The diagnostic efficacy of RIDEA and virus neutralization (VN) test was studied with 88 bovine sera. It was evident that RIDEA could eliminate the false positive reaction encountered in VN test, being more rapid and sensitive than the latter. Highy significant correlation coefficiency (r=0.76, p<0.01) was evaluated between the titers of VN test and the diameters of RIDEA. Tracheal membranes and sera collected from 96 slaughtered cattle with lesions in respiratory organs were examined to detect the antigens and the antibodies to IBRV by IFA, RIDEA and VN test. It was presented that positive rates were 32.3% in IFA, 20.8% in RIDEA and 21.9% in VN test, and that coincidence rate between RIDEA and VN test were 100'r in positive sera and 98.7% in negative sera. In conclusion, it was assumed that application of monoclonal antibody could improve the diagnostic efficacy of IBR by enhancing sensitivity and specificity of IPA, IFA and RIDEA.
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金明哲,金茂炯,金敎準,李揆丞,趙聲煥,權吳德,李憲俊 충남대학교 농업과학연구소 1987 農業技術硏究報告 Vol.14 No.2
The objective of this study was to evaluate the possibility of sex preselection by gradients methods using bovine serum albumin in rabbits. Artificial insemination with separated sperm was performed, after highly motile sperm were separated by different methods using 6%, 10% and 20% bovine serum albumin. Various characteristics of separated sperm, and the conception rate and secondary sex ratio at artificial insemination with sperm separated by different methods were compared. The results obtained were as follows. 1. The conception rate of sperm separated by bovine serum albumin gradients was higher than that of control sperm. But secondary sex ratio was not altered by this methods. 2. The sperm separated by bovine serum albumin gradients showed significantly high value in motility, percent of normal sperm and progressive motility as compared with control sperm and revealed the highest sperm recovery when separated with 6% bovine serum albumin. 3. The sperm motility, percent of normal sperm and progressive motility of the highly motile sperm frozen after being separated from raw semen with bovine serum albumin, showed significantly high value than those of control sperm.
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