Seeds as Repositories of Recombinant Proteins in Molecular Farming

Moloney, Maurice M. The Korean Society of Plant Biotechnology 2000 식물생명공학회지 Vol.27 No.4

Seeds are an ideal repository for recombinant proteins in molecular farming applications. However, in order to use plant seeds efficiently for the production of such proteins, it is necessary to understand a number of fundamental biological properties of seeds. This includes a full understanding of promoters which function in a seed-specific manner, the subcellular targeting of the desired polypeptide and the final form in which a protein is stored. Once a biologically active protein has been deposited in a seed, it is also critical that the protein can be extracted and purified efficiently. In this review, these issues are examined critically to provide a number of approaches which may be adopted for production of recombinant proteins in plants. Particular attention is paid to the relationship between subcellular localization and protein extraction and purification. The robustness and flexibility of seed-based production is illustrated by examples close to or already in commercial production.

Plant Molecular Farming Using Oleosin Partitioning Technology in Oilseeds

Moloney, Maurice-M. The Korean Society of Plant Biotechnology 1997 식물생명공학회지 Vol.24 No.4

Plant seed oil-bodies or oleosomes ate the repository of the neutral lipid stored in seeds. These organelles in many oilseeds may comprise half of the total cellular volume. Oleosomes are surrounded by a half-unit membrane of phospholipid into which are embedded proteins called oleosins. Oleosins are present at high density on the oil-body surface and after storage proteins comprise the most abundant proteins in oilseeds. Oleosins are specifically targeted and anchored to oil-bodies after co-translation on the ER. It has been shown that the amino-acid sequences responsible for this unique targeting reside primarily in the central hydrophobic tore of the oleosin polypeptide. In addition, a signal-like sequence is found near the junction of the hydrophobic domain and ann N-terminal hydrophilic / amphipathic domain. This "signal" which is uncleaved is also essential for correct targeting. Oil-bodies and their associated oleosins may be recovered by floatation centrifugation of aqueous seed extracts. This simple partitioning step results in a dramatic enrichment for oleosins in the oil-body fraction. In the light of these properties, we reasoned that it would be feasible to create fusion proteins on oil-bodies comprising oleosins and an additional valuable protein of pharmaceutical or industrial interest. It was further postulated that if these proteins were displayed on the outer surface of oil-bodies, it would be possible to release them from the purified oil-bodies using chemical or proteolytic cleavage. This could result in a simple means of recovering high-value protein from seeds at a significant (i.e. commercial) scale. This procedure has been successfully reduced to practice for a wide variety of proteins of therapeutic, industrial and food no. The utillity of the method will be discussed using a blood anticoagulant, hirudin, and industrial enzymes as key examples.

원칙중심규제 : 영국의 경험

Niamh Moloney 한국증권법학회 2017 증권법연구 Vol.18 No.1

21세기에 있어서 세계항공 및 우주공간의 효율적 이용 ( 제5차회의 : 21세기에 있어 항공우주법분야의 전망 ) : 항공사간의 협력과 경쟁에 있어서 최근의 발전동향

( John M . Moloney ) 한국항공우주법학회 1997 The International Conference on Air and Space Poli Vol.- No.-

Sarcopenia and Post-Operative Morbidity and Mortality in Patients with Gastric Cancer

O'Brien, Stephen,Twomey, Maria,Moloney, Fiachra,Kavanagh, Richard G.,Carey, Brian W.,Power, Derek,Maher, Michael M.,O'Connor, Owen J.,O'Suilleabhain, Criostoir The Korean Gastric Cancer Association 2018 Journal of gastric cancer Vol.18 No.3

Purpose: Surgical resection for gastric adenocarcinoma is associated with significant post-operative morbidity and mortality. The aim of this study was to assess the prognostic significance of sarcopenia in patients undergoing resection for gastric adenocarcinoma with respect to post-operative morbidity and survival. Materials and Methods: A retrospective analysis was conducted on a cohort of consecutive patients who underwent surgical resection for gastric adenocarcinoma between 2008 and 2014. Patient demographics, radiological parameters, and pathological data were collected. OsiriX software (Pixmeo) was used to measure skeletal muscle area, which was normalized for height to calculate skeletal muscle index. Results: A total of 56 patients (41 male, 15 female; mean age, $68.4{\pm}11.9years$) met the inclusion criteria. Of these, 36% (20 of 56) of the patients were sarcopenic pre-operatively. Both sarcopenic and non-sarcopenic patient groups were equally matched with the exception of weight and body mass index (P=0.036 and 0.001, respectively). Sarcopenia was associated with a decreased overall survival (log-rank P=0.003) and was an adverse prognostic predictor of overall survival in multivariate analysis (hazard ratio, 10.915; P=0.001). Sarcopenia was a predictor of serious in-hospital complications in multivariate analysis (odds ratio, 3.508; P=0.042). Conclusions: In patients undergoing curative resection for gastric cancer, there was a statistically significant association between sarcopenia and both decreased overall survival and serious post-operative complications. The measurement and reporting of skeletal muscle index on pre-operative computed tomography should be considered.

Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography-tandem mass spectrometry with rapid polarity switching

Whelan, M.,Kinsella, B.,Furey, A.,Moloney, M.,Cantwell, H.,Lehotay, S.J.,Danaher, M. Elsevier 2010 Journal of chromatography Vol.1217 No.27

A new UHPLC-MS/MS (ultra high performance liquid chromatography coupled to tandem mass spectrometry) method was developed and validated to detect 38 anthelmintic drug residues, consisting of benzimidazoles, avermectins and flukicides. A modified QuEChERS-type extraction method was developed with an added concentration step to detect most of the analytes at <1μgkg<SUP>-1</SUP> levels in milk. Anthelmintic residues were extracted into acetonitrile using magnesium sulphate and sodium chloride to induce liquid-liquid partitioning followed by dispersive solid phase extraction for cleanup. The extract was concentrated into dimethyl sulphoxide, which was used as a keeper to ensure analytes remain in solution. Using rapid polarity switching in electrospray ionisation, a single injection was capable of detecting both positively and negatively charged ions in a 13min run time. The method was validated at two levels: the unapproved use level and at the maximum residue level (MRL) according to Commission Decision (CD) 2002/657/EC criteria. The decision limit (CCα) of the method was in the range of 0.14-1.9 and 11-123μgkg<SUP>-1</SUP> for drugs validated at unapproved and MRL levels, respectively. The performance of the method was successfully verified for benzimidazoles and levamisole by participating in a proficiency study.

Timber-FRP composite beam subjected to negative bending

Mahbube Subhani,Anastasia Globa,Jules Moloney 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.73 No.3

In the previous studies, the authors proposed the use of laminated veneer lumber – carbon fiber reinforced polymer (LVL-CFRP) composite beams for structural application. Bond strength of the LVL-to-CFRP interface and flexural strengthening schemes to increase the bending capacity subjected to positive and negative moment were discussed in the previous works. In this article, theoretical models are proposed to predict the moment capacity when the LVL-CFRP beams are subjected to negative moment. Two common failure modes – CFRP fracture and debonding of CFRP are considered. The non-linear model proposed for positive moment is modified for negative moment to determine the section moment capacity. For the debonding based failure, previously developed bond strength model for CFRP-to-LVL interface is implemented. The theoretical models are validated against the experimental results and then use to determine the moment-rotation behaviour and rotational rigidity to compare the efficacy of various strengthening techniques. It is found that combined use of bi- and uni-directional CFRP U-wrap at the joint performs well in terms of both moment capacity and rotational rigidity.

Sarcopenia and Post-Operative Morbidity and Mortality in Patients with Gastric Cancer

Stephen O Brien,Maria Twomey,Fiachra Moloney,Richard G. Kavanagh,Brian W. Carey,Derek Power,Michael M. Maher,Owen J. OConnor,Criostoir ÓSúilleabháin 대한위암학회 2018 Journal of gastric cancer Vol.18 No.3

Purpose: Surgical resection for gastric adenocarcinoma is associated with significant post-operative morbidity and mortality. The aim of this study was to assess the prognostic significance of sarcopenia in patients undergoing resection for gastric adenocarcinoma with respect to post-operative morbidity and survival. Materials and Methods: A retrospective analysis was conducted on a cohort of consecutive patients who underwent surgical resection for gastric adenocarcinoma between 2008 and 2014. Patient demographics, radiological parameters, and pathological data were collected. OsiriX software (Pixmeo) was used to measure skeletal muscle area, which was normalized for height to calculate skeletal muscle index. Results: A total of 56 patients (41 male, 15 female; mean age, 68.4 ± 11.9 years) met the inclusion criteria. Of these, 36% (20 of 56) of the patients were sarcopenic pre-operatively. Both sarcopenic and non-sarcopenic patient groups were equally matched with the exception of weight and body mass index (P=0.036 and 0.001, respectively). Sarcopenia was associated with a decreased overall survival (log-rank P=0.003) and was an adverse prognostic predictor of overall survival in multivariate analysis (hazard ratio, 10.915; P=0.001). Sarcopenia was a predictor of serious in-hospital complications in multivariate analysis (odds ratio, 3.508; P=0.042). Conclusions: In patients undergoing curative resection for gastric cancer, there was a statistically significant association between sarcopenia and both decreased overall survival and serious post-operative complications. The measurement and reporting of skeletal muscle index on pre-operative computed tomography should be considered.

Rapid tumoritropic accumulation of systemically injected plateloid particles and their biodistribution

van de Ven, A.L.,Kim, P.,Haley, O.,Fakhoury, J.R.,Adriani, G.,Schmulen, J.,Moloney, P.,Hussain, F.,Ferrari, M.,Liu, X.,Yun, S.H.,Decuzzi, P. Elsevier Science Publishers 2012 Journal of controlled release Vol.158 No.1

Nanoparticles for cancer therapy and imaging are designed to accumulate in the diseased tissue by exploiting the Enhanced Permeability and Retention (EPR) effect. This limits their size to about 100nm. Here, using intravital microscopy and elemental analysis, we compare the in vivo localization of particles with different geometries and demonstrate that plateloid particles preferentially accumulate within the tumor vasculature at unprecedented levels, independent of the EPR effect. In melanoma-bearing mice, 1000x400nm plateloid particles adhered to the tumor vasculature at about 5% and 10% of the injected dose per gram organ (ID/g) for untargeted and RGD-targeted particles respectively, and exhibited the highest tumor-to-liver accumulation ratios (0.22 and 0.35). Smaller and larger plateloid particles, as well as cylindroid particles, were more extensively sequestered by the liver, spleen, and lungs. Plateloid particles appeared well-suited for taking advantage of hydrodynamic forces and interfacial interactions required for efficient tumoritropic accumulation, even without using specific targeting ligands.