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Helicobacter pylori infection could induce oxidative stress. Oxidative stress is involved in the pathogenesis of gastric diseases. Glutathione peroxidase 1 (GPX1), is part of the enzymatic antioxidant defense, preventing oxidative damage. The aim of the present study was to assess the association of GPX1 Pro198Leu genotypes with gastric cancer in patients with and without H. pylori infection in a population of Northern Iran. The present case-control study consisted of 50 patients with gastric cancer and 78 cancer-free subjects as controls. Extraction of DNA was performed on bioptic samples and the GPX1 genotypes were identified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The frequencies of GPX1 Pro/Pro, Pro/Leu and Leu/Leu genotypes in controls were 21.8, 71.8 and 6.4%, respectively. However, in gastric cancer patients, the frequencies of 34, 56 and 10% were observed for Pro/Pro, Pro/Leu and Leu/Leu genotypes, respectively (p = 0.185). In 38 (76%) patients infected with H. pylori, the frequencies of Pro and Leu alleles were 94.7 and 3.3%, respectively. There was a higher frequency of combined genotype of Pro/Leu + Leu/ Leu (94.7%) in H. pylori positive patients than that in patients without H. pylori infection (75%, p = 0.047). The presence of this genotype tended to increase the risk of H. pylori related gastric cancer by 5.88–fold (p = 0.069) in our population. Our findings indicated the absence of association between the GPX1 Pro198Leu polymorphism and the risk of gastric cancer in an Iranian population. However, we detected an association between H. pylori related gastric cancer with GPX1 Pro/Leu + Leu/Leu genotype.
Mohammad Jahidul Islam,Rubeca Fancy,Mohammad Shazadur Rahman,Mohammad Shamsuzzoha,Mohammad Atikul Islam,Abul Kalam Mohammad Shajedur R,Mohammad Abul Bashar 강원대학교 환경연구소 2016 Journal of the Environment Vol.11 No.1
During rainy season (June to August), 70 groundwater samples from shallow and deep aquifers were collected from Dinajpur Sadar Upazila, Dinajpur, Bangladesh. The water samples were analyzed for the major cations: (Na<SUP>+</SUP>, Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP>, K<SUP>+</SUP>, Cu<SUP>2+</SUP>, Mn<SUP>2+</SUP>, Fe<SUP>2+</SUP>) and anions: (Cl<SUP>−</SUP>, CO₃<SUP>2−</SUP>, HCO₃<SUP>−</SUP>, SO₄<SUP>2−</SUP> and NO₃<SUP>−</SUP>). Water samples contained Ca<SUP>2+</SUP>, Mg<SUP>2+</SUP> and Na<SUP>+</SUP> as dominant cations and HCO₃<SUP>−</SUP> and Cl<SUP>−</SUP> as dominant anions. Based on SARconductivity plot, the groundwater samples were classified into C2S1 and C1S1 classes. All water samples were under ‘very good’, ‘good’ and ‘permissible’ categories according to Wilcox diagram. The chemistry of groundwater is dominated by silicate weathering and (Na<SUP>+</SUP> + Mg<SUP>2+</SUP>) and (Cl<SUP>−</SUP> + SO₄<SUP>2−</SUP>) accounts for about 55% of cations and anions. The contribution of (Ca<SUP>2+</SUP> + Mg<SUP>2+</SUP>) and (Na<SUP>+</SUP> + K<SUP>+</SUP>) to total cations and HCO₃<SUP>−</SUP> indicates the domination of silicate weathering. The concentrations of the major parameters suggest that water quality of shallow and deep tube wells in the study area was satisfactory for drinking and irrigation water.
Soil and land use surveys in Puthia Upazila (small administrative unit) of the district of Rajshahi, Bangladesh under the AEZ (Agroelcological Zone)-11 were carried out to examine the changes in physical properties and land use patterns in between 1989 to 2009. Significant changes in land types, land use patterns along with crop diversification were identified. High land (HL) decreased 15.7 % in 2009 compared to 1989 survey. Medium high land (MHL) increased 124% and medium low land(MLL) decreased significantly (55%) during the survey. Sara, Tahirpur, Gopalpur, Ishwardi, Sukdebpur, Mehendigonj and Ghior soil series were identified at the time of survey. Cropping patterns were also changed in 2009 compared to 1989. In single cropped area mixed crop and relay crops were used for achieving food requirements. A large area was converted to permanent mango orchard which may be alarming for the agricultural planners, scientists and other concerns. About 278 ha agricultural land converted into non-agricultural land for making permanent buildings and other constructions. About 46.2% top soils were identified as loam soil, 38.5% as clay loam soil, 1% as clay soil, and 14.3% as miscellaneous soils in Puthia Upazila. Double and triple cropped areas were significantly increased in 2009 compared to 1989. For sufficiency of food, new crop varieties and high yielding varieties of different crops were introduced under crop diversification programs within the study period. For conservation of soil health, black gram, mung bean and other leguminous crops were introduced in some areas. Soil test based fertilizer application program would be useful to maintain soil fertility and provide continued awareness of ongoing changes in cropping patterns.
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The objectives of this study were to know the effect of repeated freeze-thaw cycles of beef on the sensory, physicochem-ical quality and microbiological assessment. The effects of three successive freeze-thaw cycles on beef forelimb were inves-tigated comparing with unfrozen fresh beef for 75 d by keeping at -20±1°C. The freeze-thaw cycles were subjected to threethawing methods and carried out to know the best one. As the number of freeze-thaw cycles increased color and odor de-clined significantly before cook within the cycles and tenderness, overall acceptability also declined among the cycles aftercook by thawing methods. The thawing loss increased and dripping loss decreased significantly (p<0.05). Water holdingcapacity (WHC) increased (p<0.05) until two cycles and then decreased. Cooking loss increased in cycle 1 and 3, but dec-reased in cycle 2. pH decreased significantly (p<0.05) among the cycles. Moreover, drip loss, cooking loss and WHC wereaffected (p<0.05) by thawing methods within the cycles. 2-Thiobarbituric acid (TBARS) value increased (p<0.05) graduallywithin the cycles and among the cycles by thawing methods. Total viable bacteria, total coliform and total yeast-mould countdecreased significantly (p<0.05) within and among the cycles in comparison to the initial count in repeated freeze-thawcycles. As a result, repeated freeze-thaw cycles affected the sensory, physicochemical and microbiological qua- lity of beef,causing the deterioration of beef quality, but improved the microbiological quality. Although repeated freeze-thaw cyclesdid not affect much on beef quality and safety but it may be concluded that repeated freeze and thaw should be minimizedin terms of beef color for commercial value and WHC and tenderness/juiciness for eating quality.
Objectives: The currently available agents for use against toxoplasmosis have serious limitations. Thus, the aim of the present study was to investigate the anti-Toxoplasma gondii (T. gondii) activities of methanol extracts of Feijoa sellowiana (F. sellowiana) (leaves and fruits), Quercus castaneifolia (Q. castaneifolia) (fruits), and Allium paradoxum (A. paradoxum) (leaves) in vitro and in vivo. Methods: Vero cells were treated with different concentrations (from 0 to 400 μg/mL) of the above extracts or with pyrimethamine at a dose of 50 mg/mL (positive control). Then, the viabilities of the T. gondii-infected cells were measured by using colorimetric MTT (3-(4, 5-dimethylthiazol-2-yl) 2, 5-diphenyltetrazolium bromide) assays. In addition, the survival rates of mice acutely infected with 2 × 104 RH strain tachyzoites of T. gondii were examined in vivo after intraperitoneal injection of the extracts at doses of 100 and 200 mg/kg/day for 5 days. Results: In the in vitro anti- T. gondii assay, the IC50 values were 12.77, 180.2, 74.73, 213.2 and 163.8 μg/mL, and the selectivity indices were 6.05, 1.31, 0.35, 0.69 and 1.30 for the F. sellowiana (leaves and fruits), Q. castaneifolia, and A. paradoxum extracts and pyrimethamine, respectively. Moreover, the mice treated with F. sellowiana (leaves and fruits) achieved better results in terms of survival than the others (P < 0.05). Conclusion: The results of the current study indicate that methanol extract of F. sellowiana has significant anti-Toxoplasma activity. Further study should be conducted to investigate the potential bioactivity of this extract through bioactivity-guided fractionation.
A discontinuous reception (DRX) operation is included in the Long Term Evolution (LTE) system to achieve power saving and prolonged battery life of the user equipment. An improvement in DRX power saving usually leads to a potential increase in the packet delay. An optimum DRX configuration depends on the current traffic, which is not easy to estimate accurately, particularly for non-real-time applications. In this paper, we propose a novel way to vary the DRX cycle length, avoiding a continuous estimation of the data traffic when only non-real-time applications are running with no active real-time applications. Because a small delay in non-real-time traffic does not essentially impact the user’s experience adversely, we deliberately allow a limited amount of delay in our proposal to attain a significant improvement in power saving. Our proposal also improves the delay in service resumption after a long period of inactivity. We use a stochastic analysis assuming an M/G/1 queue to validate this improvement.
Particle manipulation using laser beam is almost a new and contactless technique in particulate sciences. The method is based on theradiation pressure of light photons on the particles suspended in a semi-transparent fluid. Applications of the technique mainly covermicroscopic separation and detection of biological objects. In this work, a theoretical study is conducted to investigate the hydrodynamicand thermal effects on a particulate flow in a mini-channel. Laser thermal effects are studied as a result of light absorption either in fluidor dispersed phase. An analytical model is developed to be the real simulator of a test bed developed by the authors. The main objectiveof performed simulation is to determine the conditions of hydrodynamic laser-particle interaction and the contribution of thermal effects. More specifically, the criteria for size of particles and beam parameters to meet the mentioned conditions are presented. The model resultsare used for design and selection of elements in a particle manipulation system.
Wireless sensor technology has been opened up numerous opportunities to advanced health and maintenance monitoring of civil infrastructure. Compare to the traditional tactics, it offers a better way of providing relevant information regarding the condition of building structure health at a lower price. Numerous domestic buildings, especially longer-span buildings have a low frequency response and challenging to measure using deployed numbers of sensors. The way the sensor nodes are connected plays an important role in providing the signals with required strengths. Out of many topologies, the dense and sparse topologies wireless sensor network were extensively used in sensor network applications for collecting health information. However, it is still unclear which topology is better for obtaining health information in terms of greatest components, node\'s size and degree. Theoretical and computational issues arising in the selection of the optimum topology sensor network for estimating coverage area with sensor placement in building structural monitoring are addressed. This work is an attempt to fill this gap in high-rise building structural health monitoring application. The result shows that, the sparse topology sensor network provides better performance compared with the dense topology network and would be a good choice for monitoring high-rise building structural health damage.