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RISS 인기검색어
- 검색키워드
- 저자 : Mohamed Elsharkawi (검색결과 5 건)
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Elsharkawy, Mohsen Mohamed,Shimizu, Masafumi,Takahashi, Hideki,Ozaki, Kouichi,Hyakumachi, Mitsuro The Korean Society of Plant Pathology 2013 Plant Pathology Journal Vol.29 No.2
Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.
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Mohsen Mohamed Elsharkawy,Mitsuro Hyakumachi,Masafumi Shimizu,Kouichi Ozaki,Hideki Takahashi 한국식물병리학회 2013 Plant Pathology Journal Vol.29 No.2
Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI)of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes,but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ETinducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.
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Outcomes of Unibody Bifurcated Endograft and Aortobifemoral Bypass for Aortoiliac Occlusive Disease
Baker Ghoneim,Mohamed Elsherif,Mohamed Elsharkawi,Yogesh Acharya,Niamh Hynes,Wael Tawfick,Sherif Sultan 대한혈관외과학회 2020 Vascular Specialist International Vol.36 No.4
Purpose: We compared the outcomes between the total endovascular approach usinga unibody bifurcated aortoiliac endograft and the gold standard aortobifemoralbypass (ABF) surgery for the management of extensive aortoiliac occlusive disease(AIOD). Materials and Methods: This retrospective observational study compared the outcomesof endovascular technique with unibody bifurcated endograft (UBE) usingthe Endologix AFX unibody stent-graft and a standard surgical approach (ABF) inthe management of AIOD based on patient records in Western Vascular Institute,Galway University Hospital, National University of Ireland. Procedural details andoutcomes were documented to compare both groups. Results: From January 2002 to December 2018, 67 patients underwent AIOD (20UBE and 47 ABF). Both the ABF and UBE groups showed 100% immediate clinicaland technical successes without 30-day mortality. There were no statisticaldifferences in the overall survival and sustained clinical improvement betweenthe bypass and the UBE groups; however, statistically significant differences wereobserved in 3-year freedom from re-intervention and amputation-free survival. Furthermore, the mean length of the intensive care unit (ICU) stay was significantlylower in the UBE group than that in the ABF group (0.75 days vs. 3.1 days,P=0.001). Conclusion: Total endovascular reconstruction of AIOD is an alternative to invasivebypass procedures, with a shorter ICU stay.
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Control of Root Rot and Wilt Diseases of Roselle under Field Conditions
( Naglaa Hassan ),( Mohsen Mohamed Elsharkawy ),( Masafumi Shimizu ),( Mitsuro Hyakumachi ) 한국균학회 2014 Mycobiology Vol.42 No.4
Roselle (Hibiscus sabdariffa L.) is one of the most important medicinal crops in many parts of the world. In this study, the effects of microelements, antioxidants, and bioagents on Fusarium oxysporum, F. solani, and Macrophomina phaseolina, the causal pathogens of root rot and wilt diseases in roselle, were examined under field conditions. Preliminary studies were carried out in vitro in order to select the most effective members to be used in field control trials. Our results showed that microelements (copper and manganese), antioxidants (salicylic acid, ascorbic acid, and EDTA), a fungicide (Dithane M45) and biological control agents (Trichoderma harzianum and Bacillus subtilis) were significantly reduced the linear growth of the causal pathogens. Additionally, application of the previous microelements, antioxidants, a fungicide and biological control agents significantly reduced disease incidence of root rot and wilt diseases under field conditions. Copper, salicylic acid, and T. harzianum showed the best results in this respect. In conclusion, microelements, antioxidants, and biocontrol agents could be used as alternative strategies to fungicides for controlling root rot and wilt diseases in roselle.
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Flavonoids from two Cupressaceae Plants
Galal T. Maatooq,Saleh H. ElSharkawy,Mohamed S. Afifi,Jack P. N. Rosazza 한국생약학회 1998 Natural Product Sciences Vol.4 No.1
Jeceidin, jaceidin-7-O-methylether, and quercetin were isolated from-Juniperus phoenicea L. alcoholic extract, however, Sequoiaflavone was isolated from Cupressus semperiverns L. In addition, the alcoholic extracts of both plants were found to contain also kaempferol-3-O-rhamnoside, quercetrin, myrictrin, cupressuflavone. The chemical identities of the isolated compounds were established using UV, IR, ^1H-and 13^C-NMR spectroscopy.
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