Organizational Commitment of Five Working Groups of MNCs

Miao Wang,Jong-Wook Kwon,Jun-Jian Li 강원대학교 경영경제연구소 2023 Asia-Pacific Journal of Business Vol.14 No.1

Purpose - The purpose of this study is to confirm that five different working positions show different Organizational Commitment (OC) and factors influencing OC levels in the Chinese MNCs. Design/methodology/approach - The study collected data of 326 employees in different positions in Chinese MNCs abroad and in domestic country. To test hypotheses, ANOVA analysis and linearity regression are used with SPSS 26 and AMOS 26 software on the collected data. Findings - Results shows that different working groups do not show significant difference of AC and CC level. However, there are significant difference of NC among the five groups in the Chinese MNCs. Employee's position and marital status are positively related to CC and NC. Tenure is positively related to AC. Research implications or Originality - Our research offers insight for further research direction on OC difference research. It provides implication for Chinese MNCs to consider more about strategies how to stimulate host country employee's normative commitment.

Limiting the Number of Open Projects to Shorten the NPD Schedule

Wang, Miao-Ling,Yang, Chun-I,Chang, Sheng-Hung Korean Institute of Industrial Engineers 2011 Industrial Engineeering & Management Systems Vol.10 No.1

Many companies open multiple projects simultaneously due to market trends, which results in a crowding out effect because of limited resources. R&D engineers become overloaded and scheduling of product development is delayed resulting in timing misses and lost sales leads. The company in this case study (Company A), often opens up many projects simultaneously in order to respond to market needs quickly. The engineers are overloaded and, of course, the schedule is delayed. In order to identify problems, Company A began using Dr. Goldratt's Thinking Processes (TP) during new product development (NPD). When the analysis phase of TP was completed, Company A's core problem was identified as "the quantity of kick-off projects." Consequently, new rules and conditions and procedures were proposed for the opening, suspending, stopping, and closing of projects. Finally, the "Future Reality Tree" ensured that the proposed rules, conditions and procedures were set up as an available solution approved for practical application by executives. After a one-year trial run, the results showed that the Project Duration Rate was reduced by 53%, the Project Closed Rate was increased by 140% and the Project on Time Rate was increased from 10% to 68%. The above results give significant evidence of the benefits of the proposed methodology.

Limiting the Number of Open Projects to Shorten the NPD Schedule

Miao-Ling Wang,Chun-I Yang,Sheng-Hung Chang 대한산업공학회 2011 Industrial Engineeering & Management Systems Vol.10 No.1

Many companies open multiple projects simultaneously due to market trends, which results in a crowding out effect because of limited resources. R&D engineers become overloaded and scheduling of product development is delayed resulting in timing misses and lost sales leads. The company in this case study (Company A), often opens up many projects simultaneously in order to respond to market needs quickly. The engineers are overloaded and, of course, the schedule is delayed. In order to identify problems, Company A began using Dr. Goldratt"s Thinking Processes (TP) during new product development (NPD). When the analysis phase of TP was completed, Company A"s core problem was identified as "the quantity of kick-off projects." Consequently, new rules and conditions and procedures were proposed for the opening, suspending, stopping, and closing of projects. Finally, the "Future Reality Tree" ensured that the proposed rules, conditions and procedures were set up as an available solution approved for practical application by executives. After a one-year trial run, the results showed that the Project Duration Rate was reduced by 53%, the Project Closed Rate was increased by 140% and the Project on Time Rate was increased from 10% to 68%. The above results give significant evidence of the benefits of the proposed methodology.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Improving Cellulase Production in Trichoderma koningii Through RNA Interference on ace1 Gene Expression

( Wang Shao Wen ),( Miao Xing ),( Gang Liu ),( Shao Wen Yu ),( Juan Wang ),( Sheng Li Tian ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the downregulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

Efficient Mining Maximal Subspace Differential Co-expression Patterns in Matrix Datasets : a General Earthquake Analysis Approach

Miao Wang,Zhiyong Xiong,Liang Xu,Lihua Zhang,Cheng Gong,Yi Hu,Yi Lin 보안공학연구지원센터 2015 International Journal of Database Theory and Appli Vol.8 No.3

The electromagnetic anomaly observations before earthquake, have been confirmed by many cases of strong earthquakes. The analysis of earthquake magnetic anomaly is an effective approach for seismo-precursor detection. Traditional frequent mining methods for electromagnetic matrix datasets analysis often find the co-related items. However, these methods may miss the items which are differential co-related patters under different datasets. Mining these differential co-related patterns is more valuable for inferring potential knowledge. In this paper, we develop an algorithm, MSPattern, to mine maximal subspace differential co-expression patterns. MSPattern constructs a weighted undirected item-item relational graph firstly. Then all the maximal co-related patterns would be mined using item-growth method in above graph. MSPattern also utilizes several techniques for producing maximal patterns without candidate patterns maintenance. Evaluated by real electromagnetic matrix datasets and the gene expression datasets, the experimental results show our algorithm can find some potential knowledge for earthquake analysis, and MSPattern algorithm is more efficient than traditional ones. The performance of MSPattern is also evaluated by empirical p-value and gene ontology, the results show our algorithm can find statistical significant and biological differential co-expression patterns.

Identification of reference genes for normalization of gene expression in Liposcelis entomophila (Psocoptera: Liposcelididae)

Miao Shiyuan,Yang Binbin,Wang Suisui,Wang Zhengyan,Lu Yujie 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.4

Liposcelis entomophila is a nuisance pest that seriously threatens the safety of stored grains owing in part of pesticide resistance. To facilitate better control of L. entomophila, future studies need to focus on gene function characterization. This requires the identification of suitable reference genes (RGs) for reverse-transcription quantitative PCR (RT-qPCR), which is crucial for the normalization of target gene expression. Here, we evalu ated the expression stability of 12 candidate RGs in L. entomophila across six experimental conditions (devel opmental stage, population, body part, phosphine fumigation, hypoxia induction, and temperature treatment). Under developmental conditions, ACTB, HSP70, and RPL23 were the most stable RGs. Among the different populations and body parts, RPL23, EF1a, and ACTB were the most stable RGs. Comparison of gene expression among adults under different environmental conditions revealed that at least seven RGs were fit for normalizing gene expression data. Among them, RPL23, EF1a, and ACTB showed the best fit as RGs in RT-qPCR analysis. After validation, our results demonstrated that EF1a and ACTB, as well as their combination, were the ideal RGs for L. entomophila across various experimental conditions. This study provides a comprehensive RG selection sug gestion for gene expression studies of L. entomophila and will facilitate future functional genomic research on the development and tolerance of environmental factors in psocids.

How Does Foreign Aid Affect Total Fertility Rate? Panel Data Evidence

Miao Wang,Hong Zhuang 한국국제경제학회 2019 International Economic Journal Vol.33 No.4

We explore the relationship between aid and the total fertility rate in recipient countries, which is closely linked to the literature on aid and economic development. Using data on official development assistance in 86 developing countries over 1970–2015 and controlling for potential endogeneity issues, we find that foreign aid helps to lower the total fertility rate in recipient countries in general. In addition, our results suggest that development assistance is most effective in lower-income countries or countries with a lower level of human capital. We also observe considerable regional heterogeneities regarding the effect of aid on the total fertility rate.

Over-Expression of Beclin-1 Facilitates Acquired Resistance to Histone Deacetylase Inhibitor-Induced Apoptosis

Wang, Shi-Miao,Li, Xiao-Hui,Xiu, Zhi-Long Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.18

Apoptotic cell death plays a predominant role in histone deacetylase (HDAC) inhibitor-induced cytotoxicity. Nuclear morphological changes and activation of apoptotic executors are involved in CTS203-induced cell death. However, emerging issues of HDAC inhibitor-resistance have been observed in patients. Herein, MCF-7 cells were continuously exposed to CTS203 until the derived cells could proliferate normally in its presence. The newly obtained CTS203-resistant cells were nominated as MCF-7/203R. Compared to MCF-7 original cells, the MCF-7/203R cells were less sensitive to CTS203-induced apoptosis, with a minimal 6-fold higher $IC_{50}$ value. In contrast, the expression of Beclin-1 was dramatically up-regulated, positively correlated to the acquisition of CTS203-resistance. Our results revealed the participation of autophagy in acquired HDAC inhibitor-resistance and further identified Beclin-1 as a promising target for anti-drug resistance.

Synthesis of Co:MgAl2O4 nano-powders for saturable absorber

Wang Chuanyun,Yang Wei,Wang Zhiqi,Liu Bina,Li Shihua,Lu Taoa,,Li Xueren,Miao Weipeng,Luo Wei 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2

Uniform Co:MgAl2O4 nano-powders for saturable absorber were prepared using inverse-drip co-precipitation method. Effectsof the precipitant and the metal ion solution on the composition and morphology of Co:MgAl2O4 nano-powders were studied. The results show that pure Co:MgAl2O4 nano-powders can be obtained, using the mixed solution of ammonium carbonate andammonia as the precipitant. The specific surface area of the particles reached 36 m2/g and the primary particle size was 39nm according to the SEM images and BET results.