In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139

Islam, Md. Saiful,Shahik, Shah Md.,Sohel, Md.,Patwary, Noman I.A.,Hasan, Md. Anayet Korea Genome Organization 2015 Genomics & informatics Vol.13 No.2

In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera.

In Silico Structural and Functional Annotation of Hypothetical Proteins of Vibrio cholerae O139

Md. Saiful Islam,Shah Md. Shahik,Md. Sohel,Noman I. A. Patwary,Md. Anayet Hasan 한국유전체학회 2015 Genomics & informatics Vol.13 No.2

In developing countries threat of cholera is a significant health concern whenever water purification and sewage disposal systems are inadequate. Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. Hence analyzing and annotating the structure and function of hypothetical proteins is important for understanding the V. cholerae. V. cholerae O139 is the most common and pathogenic bacterial strain among various V. cholerae strains. In this study sequence of six hypothetical proteins of V. cholerae O139 has been annotated from NCBI. Various computational tools and databases have been used to determine domain family, protein-protein interaction, solubility of protein, ligand binding sites etc. The three dimensional structure of two proteins were modeled and their ligand binding sites were identified. We have found domains and families of only one protein. The analysis revealed that these proteins might have antibiotic resistance activity, DNA breaking-rejoining activity, integrase enzyme activity, restriction endonuclease, etc. Structural prediction of these proteins and detection of binding sites from this study would indicate a potential target aiding docking studies for therapeutic designing against cholera.

Characterization of grafted jute fiber using acrylate monomers pretreated with alkali

Md Ibrahim H Mondal,Md Mashiur Rahman Khan,Md. Mofakkharul Islam,Md. Saiful Islam,M. A. Rabbi 한국의류학회 2016 Fashion and Textiles Vol.3 No.1

The reactivity of lingocellulosic jute fiber to sunlight (UV) and atmospheric gases and chemicals was reduced by grafting jute with acrylate monomers, such as ethyl acrylate (EA) and 2-hydroxy ethyl methacrylate (HEMA). The grafting reaction sufficiently blocked the reactive groups of jute and thus, physically- and chemically-bound cellulosic units and on the fiber surface. The grafting gave additional strength, higher thermal stability and improved surface smoothness. Though the modified fibers showed better color fastness properties in sunlight, the dyeability of the modified fibers somewhat decreased. Grafting reaction and other characteristics of modified fibers were investigated with FT-IR, tensile tester, thermogravimeric analyser, and grey scale respectively. The change of surface morphology of the jute fiber on modification was also observed with a scanning electron microscopy. Modification of bleached jute and alkali-activated bleached jute with HEMA gave higher graft yield (26.6, 29.4 %) than that of EA modification (22.5, 24.7 %). Due to the different chemical features, HEMA-modified fiber exhibited better chemical, thermal and physical properties.

Short communication : Tube agglutination test is superior than other serological tests for diagnosis of brucellosis in small ruminants

( Md. Siddiqur Rahman ),( Nusrat Jahan ),( Mohammad Arif Hossain ),( M. J. Uddin ),( Niraj Kanti Shil ),( KBM Saiful Islam ),( Md. Shamim Ahasan ),( A. K. M. Anisur Rahman ),( Hee Jong Song ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.4

Brucella spp. are small, non-motile Gram-negative coccobacilli known to cause disease in a number of vertebrate species including humans and brucellosis is one of the world`s major zoonoses, alongside bovine tuberculosis and rabies. There are about 33.55 million goats and 1.16 million sheep in Bangladesh. The sheep and goats can significantly play an important role in the economic well being of the resource-poor farmer in Bangladesh. Sexually matured 362 female small ruminants (300 goats and 62 sheep) were examined. Approximately 3-5ml of blood was collected from the jugular vein of each animal and sera samples were prepared. Samples were then tested for brucellosis by using Rose Bengal test(RBT), plate agglutination test(PAT) and tube agglutination test(TAT). Among 362 small ruminants, irrespective of species(sheep or goat), diagnosed highest in TAT, 2.21%(n=8) and lowest both by RBT & PAT, 1.93%(n=7) and it is concluded that TAT is superior than RBT and PAT.

Genotype-Environment Interaction and Stability Analysis for Yield and Yield Contributing Characters in Soybean(Glycine max L.)

Islam, Mohammad Saiful,Newaz, Muhammad Ali,Islam, Md. Jahidul,Heo, Seong-Il,Wang, Myeong-Hyeon The Plant Resources Society of Korea 2007 한국자원식물학회지 Vol.20 No.6

GE interaction is the expression of differential genotypic adaptation across environments. GE interactions through different stability parameters and performance of the traits of genotypes were studied. The traits were days to maturity, pod length, number of pods/ plant, 100-seed weight and seed yield/plant in ten soybean genotypes across five environments. Significant differences were observed for genotypes, environments and GE interactions. Stability analysis after Eberhart and Russell's model suggested that the genotypes used in this study were all more or less responsive to environmental changes. Most of the genotypes perform better in Env.3. Based on phenotypic indices(Pi), regression ($S^2di$) genotype Garurab was found fairly stable for days to maturity. BS-23 and G-2120 may be considered as stable genotype for pod length. All the genotypes except G-2120 showed that the genotypes were relatively unstable under environmental fluctuation for the number of pod/plant. Genotype BS-23 was found most stable among all the genotypes for 100-seed weight. BS-3 and Gaurab was the most stable and desirable genotypes for seed yield in soybean.

Noise reduction of continuous wave radar and pulse radar using matched filter and wavelets

Islam, Md Saiful,Chong, Uipil Springer (Biomed Central Ltd.) 2014 EURASIP journal on image and video processing Vol.2014 No.-

Eliminating concrete cover separation of NSM strengthened beams by CFRP end anchorage

Md. Akter Hosen,Mohd Zamin Jumaat,A. B. M. Saiful Islam,Mohamed Kamruzzaman,Md. Nazmul Huda,Mahmudur Rahman Soeb 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.56 No.6

Upgrading or strengthening of existing reinforced concrete (RC) infrastructure is an emerging demand nowadays. Near Surface Mounted (NSM) technique is very promising approach for flexural strengthening of RC members. However, premature failure such as concrete cover separation failure have been a main concern in utilizing this technique. In this study, U-wrap end anchorage with carbon fiber reinforced polymer (CFRP) fabrics is proposed to eliminate the concrete cover separation failure. Experimental programs were conducted to the consequence of U-wrap end anchorage on the flexurally strengthened RC beams with NSM- steel. A total of eight RC rectangular beam specimens were tested. One specimen was kept unstrengthened as a reference; three specimens were strengthened with NSM-steel bars and the remaining four specimens were strengthened with NSM-steel bars and U-wrap end anchorage using CFRP fabrics. A 3D non-linear finite element model (FEM) was developed to simulate the flexural response of the tested specimens. It is revealed that NSM-steel (with and without end-anchors) significantly improved the flexural strength; moreover decreased deflection and strains compared with reference specimen. Furthermore, NSM-steel with end anchorage strengthened specimens revealed the greater flexural strength and improve failure modes (premature to flexure) compared with the NSM-steel without end anchorage specimens. The results also ensured that the U-wrap end anchorage completely eliminate the concrete cover separation failure.

Prevalence of Ruminal Lactic Acidosis and Clinical Assessments of Four Therapeutics in Goats of Bangladesh

Md Mahbubur Rahman,Mohammad Saiful Islam,Adam G.O,Md. Rafiqul Alam,유명조 한국임상수의학회 2014 한국임상수의학회지 Vol.31 No.3

Acidosis conditions either acute or chronic following ingestion of excessive amounts of readily fermentedcarbohydrate are great production problems for goat in Bangladesh. This study designed to investigate the prevalenceof lactic acidosis and then response to different therapeutic agents. For this purpose, 1,128 goat were examined atoutdoor District Veterinary Hospital, Faridpur, Bangladseh for treatment of which 40 goats were found positive forlactic acidosis showing 3.55% prevalence of disease. The highest occurrence found in female (4.64%) of over 3 yearsage (4.64%) in indigenous goat (2.7%). For therapeutic assessment the forty affected goats were divided into fourgroups A, B, C and D comprising of 10 animals each. Group A were given magnesium hydroxide 8% w/v at 1 g/kg body weight orally. In group B magnesium hydroxide 8% w/v at a dose as group A combination with 7.5% sodiumbicarbonate at the rate of 0.9 ml/Kg body weight intravenously administered. Goat in group D were treated with mixtureof ginger, nuxvomica, sodium carbonate, cobalt sulphate, dried ferrous sulphate and thiamin mononitrate at the rateof 1 g/kg body weight orally. Goat of group C treated with combination drugs of group A, B and D. The rectaltemperature, pulse rate, respiration rate, was performed before and after treatment. It was found that the highest recoveryin group C with an average period of 21 ± 1.8 hours. It was concluded that lactic acidosis is a common disease ofgoats and its severity can be effectively reduced by using combination drugs.

Antipsychotic dopamine receptor antagonists, cancer, and cancer stem cells

Md. Saiful Islam Roney,박성규 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.4

Cancer is one of the deadliest diseases in theworld. Despite extensive studies, treating metastatic cancersremains challenging. Years of research have linked arare set of cells known as cancer stem cells (CSCs) to drugresistance, leading to the suggestion that eradication ofCSCs might be an effective therapeutic strategy. However,few drug candidates are active against CSCs. New drugdiscovery is often a lengthy process. Drug screening hasbeen advantageous in identifying drug candidates. Currentunderstanding of cancer biology has revealed various cluesto target cancer from different points of view. Many studieshave found dopamine receptors (DRs) in various cancers. Therefore, DR antagonists have attracted a lot of attentionin cancer research. Recently, a group of antipsychotic DRantagonists has been demonstrated to possess remarkableabilities to restrain and sensitize CSCs to existingchemotherapeutics by a process called differentiationapproach. In this review, we will describe current aspectsof CSC-targeting therapeutics, antipsychotic DR antagonists,and their extraordinary abilities to fight cancer.

Occurrence, clinical signs, postmortem lesions and etiology of enterotoxaemia in Black Bengal goats

Islam, K.B.M. Saiful,Rahman, Md. Siddiqur,Ershaduzzaman, Md.,Taimur, M.J.F.A.,Song, Hee-Jong The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.4

A year round study was carried out to investigate the etiology, clinical signs, postmortem lesions and occurrence of naturally occurring enterotoxaemia in Black Bengal goats. Sixteen goats of different age and sex died in different seasons with sigh associated with enterotoxaemia made the materials of this study. Accidental access to large amount of concentrate was noted as one of the predisposing factors although few cases were reported to occur without known diet change. Younger animals(50%) and males(62.50%) were found more prone to the disease and it was likely to be more prevalent during winter(50%) followed by at rainy season(31.25%) and summer(18.75%). Diarrhoea(81.25%), dullness(56.25%), drooping of the ears(50%), anorexia(43.75%) were recorded as major clinical signs whereas enterocolitis(100%), lung edema(87.50%), fluid filled intestines(87.50%), enlarged mesenteric lymph nodes(56.52%) etc. were most common post mortem lesions found. A few cases showed lesions on heart(31.25%), brain(25%) and/or liver/spleen(18.75%) but no lesion was found on kidney. Thus the so called 'pulpy kidney' lesion was absent. Intestinal contents were subjected to conventional bacteriological culture based methods to identify the causal agents. Based on the morphological, cultural and biochemical properties the causal agent was identified as Clostridium perfringens. Despite the study was carried out at certain area it showed a clear picture of goat enterotoxaemia in terms of etiology, clinical signs, postmortem lesions and occurrence of goat enterotoxaemia in Bangladesh.