Effects of Combining Feed Grade Urea and a Slow-release Urea Product on Characteristics of Digestion, Microbial Protein Synthesis and Digestible Energy in Steers Fed Diets with Different Starch:ADF Ratios

Lopez-Soto, M.A.,Rivera-Mendez, C.R.,Aguilar-Hernandez, J.A.,Barreras, A.,Calderon-Cortes, J.F.,Plascencia, A.,Davila-Ramos, H.,Estrada-Angulo, A.,Valdes-Garcia, Y.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.2

As a result of the cost of grains, the replacement of grains by co-products (i.e. DDGS) in feedlot diets is a common practice. This change produces diets that contain a lower amount of starch and greater amount of fibre. Hypothetically, combining feed grade urea (U) with slow release urea (Optigen) in this type of diet should elicit a better synchrony between starch (high-rate of digestion) and fibre (low-rate of digestion) promoting a better microbial protein synthesis and ruminal digestion with increasing the digestible energy of the diet. Four cannulated Holstein steers ($213{\pm}4$ kg) were used in a $4{\times}4$ Latin square design to examine the combination of Optigen and U in a finishing diet containing different starch:acid detergent fibre ratios (S:F) on the characteristics of digestive function. Three S:F ratios (3.0, 4.5, and 6.0) were tested using a combination of U (0.80%) and Optigen (1.0%). Additionally, a treatment of 4.5 S:F ratio with urea (0.80% in ration) as the sole source of non-protein nitrogen was used to compare the effect of urea combination at same S:F ratio. The S:F ratio of the diet was manipulated by replacing the corn grain by dried distillers grain with solubles and roughage. Urea combination did not affect ruminal pH. The S:F ratio did not affect ruminal pH at 0 and 2 h post-feeding but, at 4 and 6 h, the ruminal pH decreased as the S:F ratio increased (linear, p<0.05). Ruminal digestion of OM, starch and feed N were not affected by urea combination or S:F ratio. The urea combination did not affect ADF ruminal digestion. ADF ruminal digestion decreased linearly (p = 0.02) as the S:F ratio increased. Compared to the urea treatment (p<0.05) and within the urea combination treatment (quadratic, p<0.01), the flow of microbial nitrogen (MN) to the small intestine and ruminal microbial efficiency were greater for the urea combination at a S:F ratio of 4.5. Irrespective of the S:F ratio, the urea combination improved (2.8%, p = 0.02) postruminal N digestion. As S:F ratio increased, OM digestion increased, but ADF total tract digestion decreased. The combination of urea at 4.5 S:F improved (2%, p = 0.04) the digestible energy (DE) more than expected. Combining urea and Optigen resulted in positive effects on the MN flow and DE of the diet, but apparently these advantages are observed only when there is a certain proportion of starch:ADF in the diet.

Effects of Combining Feed Grade Urea and a Slow-release Urea Product on Performance, Dietary Energetics and Carcass Characteristics of Feedlot Lambs Fed Finishing Diets with Different Starch to Acid Detergent Fiber Ratios

Estrada-Angulo, A.,Lopez-Soto, M.A.,Rivera-Mendez, C.R.,Castro, B.I.,Rios, F.G.,Davila-Ramos, H.,Barreras, A.,Urias-Estrada, J.D.,Zinn, R.A.,Plascencia, A. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.12

Recent findings have shown that microbial nitrogen flow and digestible energy of diets are increased when urea is combined with a slow-release urea (SRU) in diets with a starch to acid detergent fibre ratio (S:F) 4:1. This affect is attributable to enhanced synchrony between ruminal N availability for microbial growth and carbohydrate degradation. To verify the magnitude of this effects on lamb performance, an experiment was conducted to evaluate the effects of combining urea and a SRU in diets containing S:F ratios of 3:1, 4:1, or 5:1 on performance, dietary energetics and carcass characteristics of finishing lambs. For that, 40 Pelibuey${\times}$Katahdin lambs ($36.65{\pm}3kg$) were assigned to one of five weight groupings in 20 pens (5 repetition/treatments). The S:F ratio in the diet was manipulated by partially replacing the corn grain and dried distiller's grain with solubles by forage (wheat straw) and soybean meal to reach S:F ratios of 3:1, 4:1 or 5:1. An additional treatment of 4:1 S:F ratio with 0.8% urea as the sole source of non-protein nitrogen was used as a reference for comparing the effect of urea combination vs. conventional urea at the same S:F ratio. There were no treatment effects on dry matter intake (DMI). Compared the urea combination vs urea at the same S:F ratio, urea combination increased (p<0.01) average daily gain (ADG, 18.3%), gain for feed (G:F, 9.5%), and apparent energy retention per unit DMI (8.2%). Irrespective of the S:F ratio, the urea combination improved the observed-to-expected dietary ratio and apparent retention per unit DMI was maximal (quadratic effect, $p{\leq}0.03$) at an S:F ratio of 4:1, while the conventional urea treatment did not modify the observed-to-expected net energy ratio nor the apparent retention per unit DMI at 4:1 S:F ratio. Urea combination group tended (3.8%, p = 0.08) to have heavier carcasses with no effects on the rest of carcass characteristics. As S:F ratio increased, ADG, G:F, dietary net energy, carcass weight, dressing percentage and longissimus thoracis (LM) area increased linearly ($p{\leq}0.02$). Combining urea and a slow-release urea product results in positive effects on growth performance and dietary energetics, but the best responses are apparently observed when there is a certain proportion (S:F ratio = 4:1) of starch to acid detergent fibre in the diet.

Directly comparing GW150914 with numerical solutions of Einstein’s equations for binary black hole coalescence

Abbott, B. P.,Abbott, R.,Abbott, T. D.,Abernathy, M. R.,Acernese, F.,Ackley, K.,Adams, C.,Adams, T.,Addesso, P.,Adhikari, R. X.,Adya, V. B.,Affeldt, C.,Agathos, M.,Agatsuma, K.,Aggarwal, N.,Aguiar, O. American Physical Society 2016 Physical Review D Vol.94 No.6

<P>We compare GW150914 directly to simulations of coalescing binary black holes in full general relativity, including several performed specifically to reproduce this event. Our calculations go beyond existing semianalytic models, because for all simulations-including sources with two independent, precessing spins - we perform comparisons which account for all the spin-weighted quadrupolar modes, and separately which account for all the quadrupolar and octopolar modes. Consistent with the posterior distributions reported by Abbott et al. [Phys. Rev. Lett. 116, 241102 (2016)] (at the 90% credible level), we find the data are compatible with a wide range of nonprecessing and precessing simulations. Follow-up simulations performed using previously estimated binary parameters most resemble the data, even when all quadrupolar and octopolar modes are included. Comparisons including only the quadrupolar modes constrain the total redshifted mass M-z epsilon [64 M-circle dot - 82 M-circle dot], mass ratio 1/q = m(2)/m(1) epsilon [0.6; 1], and effective aligned spin chi(eff) epsilon [-0.3, 0.2] where chi(eff) = (S-1/m(1)+S-2/m(2)). (L) over cap /M. Including both quadrupolar and octopolar modes, we find the mass ratio is even more tightly constrained. Even accounting for precession, simulations with extreme mass ratios and effective spins are highly inconsistent with the data, at any mass. Several nonprecessing and precessing simulations with similar mass ratio and chi(eff) are consistent with the data. Though correlated, the components' spins (both in magnitude and directions) are not significantly constrained by the data: the data is consistent with simulations with component spin magnitudes a(1,2) up to at least 0.8, with random orientations. Further detailed follow-up calculations are needed to determine if the data contain a weak imprint from transverse (precessing) spins. For nonprecessing binaries, interpolating between simulations, we reconstruct a posterior distribution consistent with previous results. The final black hole's redshifted mass is consistent with M-f,M-z in the range 64.0 M-circle dot - 73.5 M-circle dot and the final black hole's dimensionless spin parameter is consistent with a(f) = 0.62-0.73. As our approach invokes no intermediate approximations to general relativity and can strongly reject binaries whose radiation is inconsistent with the data, our analysis provides a valuable complement to Abbott et al.</P>

Fusarium Species from Sorghum in Thailand

Mohamed Nor, Nik M.I.,Salleh, Baharuddin,Leslie, John F. The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.4

Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number ($N_e$) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.

CANDELS Sheds Light on the Environmental Quenching of Low-mass Galaxies

Guo, Yicheng,Bell, Eric F.,Lu, Yu,Koo, David C.,Faber, S. M.,Koekemoer, Anton M.,Kurczynski, Peter,Lee, Seong-Kook,Papovich, Casey,Chen, Zhu,Dekel, Avishai,Ferguson, Henry C.,Fontana, Adriano,Giavalis American Astronomical Society 2017 ASTROPHYSICAL JOURNAL LETTERS - Vol.841 No.2

<P>We investigate the environmental quenching of galaxies, especially those with stellar masses (M-*) < 10(9.5) Me-circle dot, beyond the local universe. Essentially all local low-mass quenched galaxies (QGs) are believed to live close to massive central galaxies, which is a demonstration of environmental quenching. We use CANDELS data to test whether or not such a dwarf QG-massive central galaxy connection exists beyond the local universe. For this purpose, we only need a statistically representative, rather than complete, sample of low-mass galaxies, which enables our study to z greater than or similar to 1.5. For each low-mass galaxy, we measure the projected distance (d(proj)) to its nearest massive neighbor (M-* > 10(10.5) M-circle dot) within a redshift range. At a given z and M-*, the environmental quenching effect is considered to be observed if the d(proj) distribution of QGs (d(proj)(Q)) is significantly skewed toward lower values than that of star-forming galaxies (d(proj)(SF)). For galaxies with 10(8) M-circle dot < M-* < 10(10) M-circle dot, such a difference between d(proj)(Q) and d(proj)(SF) is detected up to z similar to 1. Also, about 10% of the quenched galaxies in our sample are located between two and four virial radii (R-Vir) of the massive halos. The median projected distance from low-mass QGs to their massive neighbors, d(proj)(Q)/R-Vir, decreases with satellite M-* at M-* less than or similar to 10(9.5) M-circle dot, but increases with satellite M-* at M-* greater than or similar to 10(9.5) M-circle dot. This trend suggests a smooth, if any, transition of the quenching timescale around M-* similar to 10(9.5) M-circle dot at 0.5 < z < 1.0.</P>

Fusarium Species from Sorghum in Thailand

Nik M. I. Mohamed Nor,Baharuddin Salleh,John F. Leslie 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.4

Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number (Ne) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.

Hyaluronidase-inhibitory activities of glycosaminoglycans from Liparis tessellatus eggs

Ticar, B.F.,Rohmah, Z.,Mussatto, S.I.,Lim, J.M.,Park, S.,Choi, B.D. Applied Science Publishers 2017 Carbohydrate polymers Vol.161 No.-

Polysaccharide fractions isolated from L. tessellatus eggs were purified and eluted using the DEAE-sepharose fast flow column. These were collected, tested and pooled based on their sugars content: F1, F2, and F3 which contain 26.8, 23.3, and 20.2% sulfated glycans; 34.5, 38.2, and 45.0% uronic acids; and 23.5, 19.0, and 7.5% acetylhexosamines and hexosamines, respectively. Hyaluronidase inhibitory effects of the fractions are in the order F3>F2>F1>Ascorbic acid, with F3 having the highest inhibition among the fractions and that of the standard, ascorbic acid. The electrospray ionization tandem mass spectrometry (ESI-MS/MS) confirmed the presence of uronic acids on F3, which could be a <SUP>0,2</SUP>A<SUB>2</SUB> fragment plus loss of methyl group which is very common among non-methylated, sulfated disaccharides.

Searches for W′ bosons decaying to a top quark and a bottom quark in proton-proton collisions at 13 TeV

Sirunyan, A. M.,Tumasyan, A.,Adam, W.,Ambrogi, F.,Asilar, E.,Bergauer, T.,Brandstetter, J.,Brondolin, E.,Dragicevic, M.,Erö,, J.,Flechl, M.,Friedl, M.,Frü,hwirth, R.,Ghete, V. M.,Grossmann, J. Institute of Physics Pub 2017 The journal of high energy physics Vol.2017 No.8

<P>Searches are presented for heavy gauge bosons decaying into a top and a bottom quark in data collected by the CMS experiment at root s = 13TeV that correspond to an integrated luminosity of 2.2 and 2.6 fb 1 in the leptonic and hadronic analyses, respectively. Two final states are analyzed, one containing a single electron, or muon, and missing transverse momentum, and the other containing multiple jets and no electrons or muons. No evidence is found for a right-handed W' boson (W'(R)) and the combined analyses exclude at 95% confidence level W'(R) with masses below 2.4TeV if M-W'R >> M-vR (mass of the right-handed neutrino), and below 2.6TeV if M-W'R < M-vR. The results provide the most stringent limits for right-handed W' bosons in the top and bottom quark decay channel.</P>

AKARI mid-infrared slitless spectroscopic survey of star-forming galaxies at <i>z</i> ≲ 0.5

Ohyama, Y.,Wada, T.,Matsuhara, H.,Takagi, T.,Malkan, M.,Goto, T.,Egami, E.,Lee, H.-M.,Im, M.,Kim, J.H.,Pearson, C.,Inami, H.,Oyabu, S.,Usui, F.,Burgarella, D.,Mazyed, F.,Imanishi, M.,Jeong, W.-S.,Miya EDP Sciences 2018 Astronomy and astrophysics Vol.618 No.-

<P><I>Context.</I> Deep mid-infrared (MIR) surveys have revealed numerous strongly star-forming galaxies at redshift <I>z</I> ≲ 2. Their MIR fluxes are produced by a combination of continuum and polycyclic aromatic hydrocarbon (PAH) emission features. The PAH features can dominate the total MIR flux, but are difficult to measure without spectroscopy.</P><P><I>Aims.</I> We aim to study star-forming galaxies by using a blind spectroscopic survey at MIR wavelengths to understand evolution of their star formation rate (SFR) and specific SFR (SFR per stellar mass) up to <I>z</I> ≃ 0.5, by paying particular attention to their PAH properties.</P><P><I>Methods.</I> We conducted a low-resolution (<I>R</I> ≃ 50) slitless spectroscopic survey at 5-13 <I>μ</I>m of 9 <I>μ</I>m flux-selected sources (>0.3 mJy) around the north ecliptic pole with the infrared camera (IRC) onboard AKARI. After removing 11 AGN candidates by using the IRC photometry, we identify 48 PAH galaxies with PAH 6.2, 7.7, and 8.6 <I>μ</I>m features at <I>z</I> < 0.5. The rest-frame optical-MIR spectral energy distributions (SEDs) based on CFHT and IRC imaging covering 0.37-18 <I>μ</I>m were produced, and analysed in conjunction with the PAH spectroscopy. We defined the PAH enhancement by using the luminosity ratio of the 7.7 <I>μ</I>m PAH feature over the 3.5 <I>μ</I>m stellar component of the SEDs.</P><P><I>Results.</I> The rest-frame SEDs of all PAH galaxies have a universal shape with stellar and 7.7 <I>μ</I>m bumps, except that the PAH enhancement significantly varies as a function of the PAH luminosities. We identify a PAH-enhanced population at <I>z</I> ≳ 0.35, whose SEDs and luminosities are typical of luminous infrared galaxies. They show particularly larger PAH enhancement at high luminosity, implying that they are vigorous star-forming galaxies with elevated specific SFR. Our composite starburst model that combines a very young and optically very thick starburst with a very old population can successfully reproduce most of their SED characteristics, although we cannot confirm this optically think component from our spectral analysis.</P>

Ingestive Behavior of Heifers Supplemented with Glycerin in Substitution of Corn on Brachiaria brizantha Pasture

Facuri, L.M.A.M.,Silva, Roberio Rodrigues,da Silva, F.F.,de Carvalho, G.G.P.,Sampaio, C.B.,Mendes, F.B.L.,Lisboa, M.M.,Barroso, D.S.,Carvalho, V.M.,Pereira, M.M.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.11

The objective was to evaluate the ingestive behavior of crossbred heifers finished on a Brachiaria brizantha cv. Marandu pasture receiving four levels of glycerin in their supplementation. Thirty-six crossbred heifers with average initial weight of $264.83{\pm}3.83kg$ and 20 months of age were distributed into a completely randomized design with four treatments and nine replications: control (0%), 4.82%, 10.12%, and 15.56% glycerin in the dry matter. The grazing time reduced linearly (p<0.05), whereas the time spent on activities like rumination, idleness, trough and total chewing time were quadratically affected (p<0.05). Bite rate and number of bites/day were quadratically influenced (p<0.05). The number of bites/swallowed cud and the number of bites/minute, however, increased linearly (p<0.05). Although the time spent on each cud and number of chews per cud were not affected (p>0.05).The number of rumination periods reduced linearly (p<0.05), whereas the number of grazing, idle and trough periods, and the times per grazing, idle, rumination and trough periods were quadratically affected (p<0.05). The feed and rumination efficiencies of the dry matter, non-fibrous carbohydrates, pasture dry matter and concentrate were quadratically affected (p>0.05) whereas the feed efficiency of neutral detergent fiber reduced linearly (p<0.05). Addition of glycerin in substitution of corn in supplements for animals managed on pastures does not influenced feed intake, but reduces the grazing time and increases the idle time. The supplementation also improves feed and rumination efficiencies.