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Imam, Hasan,Khan, Mohsin,Gokhale, Nandan S.,McIntyre, Alexa B. R.,Kim, Geon-Woo,Jang, Jae Young,Kim, Seong-Jun,Mason, Christopher E.,Horner, Stacy M.,Siddiqui, Aleem National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.35
<P>N6-methyladenosine (m(6)A) RNA methylation is the most abundant epitranscriptomic modification of eukaryotic messenger RNAs (mRNAs). Previous reports have found m(6)A on both cellular and viral transcripts and defined its role in regulating numerous biological processes, including viral infection. Here, we show that m(6)A and its associated machinery regulate the life cycle of hepatitis B virus (HBV). HBV is a DNA virus that completes its life cycle via an RNA intermediate, termed pregenomic RNA (pgRNA). Silencing of enzymes that catalyze the addition of m(6)A to RNA resulted in increased HBV protein expression, but overall reduced reverse transcription of the pgRNA. We mapped the m(6)A site in the HBV RNA and found that a conserved m(6)A consensus motif situated within the epsilon stem loop structure, is the site for m(6)A modification. The epsilon stem loop is located in the 3' terminus of all HBV mRNAs and at both the 5' and 3' termini of the pgRNA. Mutational analysis of the identified m(6)A site in the 5' epsilon stem loop of pgRNA revealed that m(6)A at this site is required for efficient reverse transcription of pgRNA, while m(6)A methylation of the 3' epsilon stem loop results in destabilization of all HBV transcripts, suggesting that m(6)A has dual regulatory function for HBV RNA. Overall, this study reveals molecular insights into how m(6)A regulates HBV gene expression and reverse transcription, leading to an increased level of understanding of the HBV life cycle.</P>
A. A. Al-Amiery,Firas F. Sayyid,Ali M. Mustafa,Slafa I. Ibrahim,Mustafa K. Mohsin,Mahdi M. Hanoon,Mohammed H. H. Al-Kaabi,A. A. H. Kadhum,Wan Nor Roslam Wan Isahak 한국부식방식학회 2023 Corrosion Science and Technology Vol.22 No.2
Due to continuous promotion of green alternatives to toxic petrochemicals by government policies,research efforts towards the development of green corrosion inhibitors have intensified recently. The objectiveof the current work was to develop novel green and sustainable corrosion inhibitors derived from 4-aminoantipyrine to effectively prevent corrosion of mild steel in corrosive environments. Gravimetricmethods were used to investigate corrosion inhibition of 4-((furan-2-ylmethylene)amino)antipyrine (FAP)and 4-((pyridin-2-ylmethylene)amino)antipyrine (PAP) for mild steel in 1 M HCl. FAP and PAP were subjectedto quantum chemical calculations using density functional theory (DFT). DFT was used to determinethe mechanism of mild steel corrosion inhibition using inhibitors tested in HCl. Results demonstratedthat these tested inhibitors could effectively inhibit mild steel corrosion in 1.0 M HCl. At 0.0005 M, theseinhibitors’ efficiencies for FAP and PAP were 93.3% and 96.5%, respectively. The Langmuir adsorptionisotherm was obeyed by these inhibitors on the mild steel surface. Values of adsorption free energies,, revealed that FAP followed chemical and physical adsorptions.
Electrochemical corrosion study of helium ions implanted Zircaloy-4 in chloride media
Rafique, Mohsin,Khan, Atika,Afzal, Naveed,Farooq, Ameeq,Imran, M. Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.3
In this work, an attempt is made to improve the electrochemical corrosion resistance of Zircaloy-4 by helium ions implantation. For this purpose, the Zircaloy-4 was implanted with 300 keV helium ions of fluences 1 × 10<sup>13</sup>, 1 × 10<sup>15</sup>, and 1 × 10<sup>16</sup> ions-cm<sup>-2</sup> by using Pelletron Accelerator. Electrochemical tests of pristine and ion-implanted samples were performed in NaCl solution and their potentiodynamic polarization curves were obtained. The results showed enhancement of the corrosion resistance of Zircaloy-4 after helium ions implantation. The corrosion rate and current density of the material were significantly reduced by the helium implantation. The decrease in corrosion parameters was attributed to helium ions diffusion inside Zircaloy-4 that reduced the electrons flow from the samples.
Saikh M. Wabaidur,Zeid A. AlOthman,Masoom R. Siddiqui,Kazi Mohsin,Leda G. Bousiakou,George A. Karikas 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.24 No.-
Ultra performance liquid chromatography–mass spectrometry (UPLC–MS) method has been developed for the simultaneous determination of pharmaceuticals in industrial waste water samples. The binary mobile phase was used a mixture of acetonitrile and 0.1% aqueous solution of formic acid at a flow rate of 0.4 ml min1. Identification and quantitation of the analyzed drug samples were carried out using mass spectrometer equipped with electrospray ionization source operated in negative ionization mode. The linear range of the proposed method was found to be in the range of 18–270 ng ml1 for all drugs with limit of detection in the range of 1.25 to 1.89 ng ml1.
Micro-hardness and Young's modulus of a thermo-mechanically processed biomedical titanium alloy
Mohammed, Mohsin Talib,Khan, Zahid A.,M., Geetha,Siddiquee, Arshad N. Techno-Press 2014 Biomaterials and biomedical engineering Vol.1 No.3
This paper presents a study on the influence of different thermo-mechanical processing (TMP) parameters on some required properties such as micro-hardness and Young's modulus of a novel near ${\beta}$ alloy Ti-20.6Nb-13.6Zr-0.5V (TNZV). The TMP scheme comprises of hot working above and below ${\beta}$ phase, solutionizing treatment above and below ${\beta}$ phase coupled with different cooling rates. Factorial design of experiment is used to systematically collect data for micro-hardness and Young's modulus. Validity of assumptions related to the collected data is checked through several diagnostic tests. The analysis of variance (ANOVA) is used to determine the significance of the main and interaction effects. Finally, optimization of the TMP process parameters is also done to achieve optimum values of the micro-hardness and Young's modulus.
Design, Control & Performance Analysis of Two-link Robotic Manipulator System
S. M. Hasibul Hoq,Saifullah Shohel,Dr. Md. Shafiul Alam,Mukimut Salat,Mohsin Ahmad 대한전자공학회 2017 대한전자공학회 학술대회 Vol.2017 No.1
This paper focuses on design of two-link flexible joint robotic manipulator and its practical aspects, control design and performance analysis. The controller is build using Arduino board. The robotic manipulator can be controlled via smartphone android app and transmitter. The precision of the entire system is demonstrated practically. Special control design features is introduced in the manipulator system, added new dimension in two-link robot technology. Performance & budget analysis is shown to evaluate how the research is different from the conventional system. The efficiency of the entire system is established through both simulation and practical studies. The end result denotes the proposed control system achieves much better regulation. End of the research performance analysis of the practical prototype system ensures sustainability, affordability and reliability. This paper also enables the immense possible research scope among home and industrial robots.
Concurrent Isolation of 3 Distinct Cardiac Stem Cell Populations From a Single Human Heart Biopsy
Monsanto, Megan M.,White, Kevin S.,Kim, Taeyong,Wang, Bingyan J.,Fisher, Kristina,Ilves, Kelli,Khalafalla, Farid G.,Casillas, Alexandria,Broughton, Kathleen,Mohsin, Sadia,Dembitsky, Walter P.,Sussman, Grune & Stratton 2017 Circulation research Vol.121 No.2
<P><B><U>Rationale:</U></B></P><P>The relative actions and synergism between distinct myocardial-derived stem cell populations remain obscure. Ongoing debates on optimal cell population(s) for treatment of heart failure prompted implementation of a protocol for isolation of multiple stem cell populations from a single myocardial tissue sample to develop new insights for achieving myocardial regeneration.</P><P><B><U>Objective:</U></B></P><P>Establish a robust cardiac stem cell isolation and culture protocol to consistently generate 3 distinct stem cell populations from a single human heart biopsy.</P><P><B><U>Methods and Results:</U></B></P><P>Isolation of 3 endogenous cardiac stem cell populations was performed from human heart samples routinely discarded during implantation of a left ventricular assist device. Tissue explants were mechanically minced into 1 mm<SUP>3</SUP> pieces to minimize time exposure to collagenase digestion and preserve cell viability. Centrifugation removes large cardiomyocytes and tissue debris producing a single cell suspension that is sorted using magnetic-activated cell sorting technology. Initial sorting is based on tyrosine-protein kinase Kit (c-Kit) expression that enriches for 2 c-Kit<SUP>+</SUP> cell populations yielding a mixture of cardiac progenitor cells and endothelial progenitor cells. Flowthrough c-Kit<SUP>−</SUP> mesenchymal stem cells are positively selected by surface expression of markers CD90 and CD105. After 1 week of culture, the c-Kit<SUP>+</SUP> population is further enriched by selection for a CD133<SUP>+</SUP> endothelial progenitor cell population. Persistence of respective cell surface markers in vitro is confirmed both by flow cytometry and immunocytochemistry.</P><P><B><U>Conclusions:</U></B></P><P>Three distinct cardiac cell populations with individualized phenotypic properties consistent with cardiac progenitor cells, endothelial progenitor cells, and mesenchymal stem cells can be successfully concurrently isolated and expanded from a single tissue sample derived from human heart failure patients.</P>