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Prognostic Significance of 14-3-3γ Overexpression in Advanced Non-Small Cell Lung Cancer
Raungrut, Pritsana,Wongkotsila, Anusara,Lirdprapamongkol, Kriengsak,Svasti, Jisnuson,Geater, Sarayut Lucien,Phukaoloun, Monlika,Suwiwat, Supaporn,Thongsuksai, Paramee Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8
The 14-3-3 protein has been shown to be involved in the cancer process. However, there is no understanding of the relationship between 14-3-$3{\gamma}$ (14-3-3 gamma) expression and prognosis in advanced non-small cell lung cancer. In this study, we therefore investigated the association between protein levels by immunohistochemistry and clinicopathological features of advanced NSCLC patients. Survival curves were estimated using the Kaplan-Meier method and tested by log-rank. Multivariate analysis was conducted with the Cox's regression model to determine independence of factors. p values less than 0.05 were considered significant. A total 153 patients were studied, with 54.3% being stage III and 45.8% stage IV. Fifty-one cases (33.3%) were squamous cell carcinomas, and 98 cases (64.1%) were adenocarcinomas. High 14-3-$3{\gamma}$ expression was seen in 59.5% and significantly correlated with lymph node metastasis (p=0.010) and distant metastasis (p=0.017). On Kaplan-Meier analysis, high 14-3-$3{\gamma}$ expression was associated with poorer survival with a marginal trend toward significance (p=0.055). On multivariate analysis, age, treatment, and 14-3-$3{\gamma}$ expression proved to be independent prognostic parameters. In vitro experiments indicated that 14-3-$3{\gamma}$ overexpression also played a potential role in cancer invasion. In conclusion, our data suggest that 14-3-$3{\gamma}$ overexpression is associated with invasion and a poor prognosis. Therefore, 14-3-$3{\gamma}$ may be a potential prognostic marker of advanced non-small cell lung cancer.
Piyachat Chuysinuan,Chalinan Pengsuk,Kriengsak Lirdprapamongkol,Supanna Techasakul,Jisnuson Svasti,Patcharakamon Nooeaid 한국섬유공학회 2019 Fibers and polymers Vol.20 No.1
Tissue engineering involves a multifunctional temporary matrix which regulates tissue regeneration through controlled drug release against infections. A nanofibrous core-sheath structured scaffold comprising a tetracycline-loaded alginate/soy protein isolate (TCH-Alg/SPI) as a core and polycaprolactone (PCL) as a sheath was developed using co-axial electrospinning. Coverage of hydrophobic PCL on TCH-Alg/SPI fibers enhanced their structural stability in aqueous solutions as unsheathed fibers rapidly decomposed and provided fast drug release. Core-sheath fibers exhibited an initial burst release at ~49 % after 6 h of immersion in phosphate-buffered saline (PBS) solution and the sustain release reached ~80 % of total loaded drug on day 14. Release characteristics of TCH-Alg/SPI fibers without PCL covering showed immediate drug release within 48 h. Core-sheath fibers investigated by disk diffusion exhibited antibacterial properties against Staphylococcus aureus and Escherichia coli. The non-toxicity of core-sheath fibers was confirmed by an indirect cytotoxicity test using human dermal fibroblasts which showed compatibility and high cell viability of up to 100 % in treated cells. TCH-Alg/SPI-PCL core-sheath fibers show promise as tissue engineering scaffolds which can act as temporary templates for tissue regeneration and exhibit antibiotic release functions against infections caused by pathogenic microorganisms.