Paclitaxel inhibits proliferation and promotes apoptosis through regulation ROS and endoplasmic reticulum stress in osteosarcoma cell

Li Ming,Yin Liming,Wu Lili,Zhu Yunsen,Wang Xi 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4

Background Paclitaxel is a chemotherapeutic drug for cancer, which is isolated from the Pacifc yew tree. However, and the molecular mechanism and the antitumor efects of paclitaxel on osteosarcoma cell remain to be explored. Objective The aim of our study was to explore the possible molecular mechanisms of apoptosis in osteosarcoma induced by paclitaxel. Results Paclitaxel can obviously decrease the proliferation of HOS-732 cells in a dose-dependent manner. Paclitaxel could induce the cell cycle arrest at the G2/M-phase and decreases the CDK5 and CCNE1 expression in HOS-732 cells. Paclitaxel promotes cell apoptosis in HOS-732 cells, which may be contacted to the decreasing of Bcl-2 protein expression. Further, the production of ROS in HOS-732 cells was remarkably increased with the increasing concentration of paclitaxel. Moreover, paclitaxel induces the ER-stress related gene and protein expression (GRP79, DDIT3 mRNA and GRP78, XBP-1 s, IRE1α protein expression) in osteosarcoma cells. Conclusion Paclitaxel can inhibit the proliferation of HOS-732 cells and increase ROS and ER-stress response to promote cell apoptosis, suggesting that paclitaxel may represent a new therapeutic option for the treatment and prevention osteosarcoma.

Aerodynamic performance analysis of partial admission dual row control stage at different working conditions

Liming Song,Jun Li,Kesong Wen 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.1

Aerodynamic performance of partial admission dual row control stage at the rated and off-designed operating conditions was numericallyinvestigated using three-dimensional Reynolds-averaged Navier-Stokes (RANS) and k -e turbulence model. The full scale computationalmodel includes the four nozzle boxes, full first and second row rotor blade, as well as two admission guided vanes with considerationof the rotor tip clearance and stator diaphragm gland. The numerical results of the mass flow rate, power output and aerodynamicefficiency of the dual row control stage at the rated and off-designed conditions are well in agreement with the experimental data. The obtained results at rated condition show that the blocking segmental arc of guided vane increased the exit pressure of the upstreamnozzle, which reduced the mass flow rate and changed the aerodynamic performance of the nozzle. The circumferential non-uniformityof aerodynamic parameters and partial admission losses increases with the decrease in the admission degree for the computed three operatingconditions. The analysis of axial steam velocity shows that the trailing shedding vortex and the complex flow vortex in flow passageshave a significant impact on the magnitude and direction of the axial steam velocity. Furthermore, the partial admission degreechanged the proportions of the power output of two rotor blade rows. The lower partial admission degree leads to a larger proportion ofthe power output of the first rotating blade row. The detailed flow pattern in the partial admissions dual row control stage at differentoperating conditions is also illustrated.

Event-triggered Iterative Learning Control for Perfect Consensus Tracking of Non-identical Fractional Order Multi-agent Systems

Liming Wang,Guoshan Zhang 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.3

This paper is devoted to the perfect tracking problem of output consensus for a class of non-identical fractional order multi-agent systems (NIFOMASs), in which different agents have different and unknown fractional orders and dynamic functions. For the NIFOMASs including one leader agent and multiple follower agents, by designing the event-triggered mechanism along an iteration axis and introducing it into the iterative learning controller, an event-triggered iterative learning consensus protocol is proposed to reduce the number of controller update and to save the communication resource. By analyzing the convergence of learning process, the sufficient conditions are derived to guarantee that the output consensus tracking can be perfectly achieved over the finite time interval as the iteration step goes to infinity. Finally, three numerical examples are presented to demonstrate the effectiveness and wide application scope of the proposed control strategy.

A Parametric and Accurate CAD Model of Flat End Mills Based on Its Grinding Operations

Liming Wang,Lin Kong,Jian-Feng Li,Zezhong Chen 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.10

End mills are widely used in CNC machining. However, the complex structures of end mills make it difficult to define the CAD model which determines the quality and performance of virtual cutting tests. This paper presents an accurate CAD model of end mill through analyzing kinematic models of grinding processes. Initially, a common parametric representation of grinding wheel is given. Base on the given wheel profile and the design parameters of cutters including the rake angle, core diameters and pitch angle, the representation of helix flute surface is first calculated using contact theory and also the formulation of rake angle and pitch angle are investigated. Next, four design parameters are used to control the shape of the gash which is formed via Boolean operation between the given wheel and end mill. Similarly, the peripheral edges and end edges are swept using grinding simulation. Finally, integrating all the above processes, a parametric CAD model of end mill is represented. The simulation results showed that the proposed CAD model could achieve 1e-3 mm and 2e-2 deg. in accuracy, which can be used to evaluate the grinding process or improve the future FEA analysis of end-mills.

Noun Phrase Complexity in EFL Academic Writing : A Corpus-based Study of Postgraduate Academic Writing

Liming Liu,Lan Li 아시아영어교육학회 2016 The Journal of Asia TEFL Vol.13 No.1

Noun phrase (NP) centered structures are distinctive syntactic devices in academic discourse. The commonly employed subordination-based complexity measures cannot adequately capture the development of syntactic complexity of noun phrases expected of advanced student academic writing (Biber, Gray, & Poonpon, 2011). Following the call for more research in this area (e.g. Lu, 2011, p. 57), the current study compared noun phrase complexity in two corpora: one is a corpus of MA dissertations written by Chinese EFL students and the other comprises published research articles in applied linguistics journals. The study examined overall noun phrase complexity using an automatic syntactic complexity analyzer and specifically identified features of one aspect of NP complexity: NP postmodification. The quantitative results were further contextualized in a textual analysis of excerpts from the two corpora for demonstrating the significance of NP complexity to the establishment of discourse coherence. Results of the analyses showed significant underdevelopment of NP postmodification complexity in student writing relative to published texts, meanwhile explicating the circumstances under which the difference is meaningful. Implications of the findings for the teaching of EFL academic writing were also discussed.

Polymorphisms of Inflammatory Cytokine Genes and Risk for Intracranial Aneurysm: A Systematic Review and Meta-Analysis

Liming Hu,Bingyang Li,Xin Liao,Junxia Yan 연세대학교의과대학 2020 Yonsei medical journal Vol.61 No.5

Purpose: Inflammatory cytokines are thought to be involved in the pathogenesis of intracranial aneurysm (IA), although results among studies in the literature are inconsistent. This article sought to review studies on the associations among polymorphisms in inflammatory cytokine genes and IA risk and to provide recommendations for future research. Materials and Methods: A systematic search of PubMed, Embase, and Web of Science was conducted up to August 4, 2019. The associations between polymorphisms of inflammatory cytokine genes and IA risk were estimated by pooled odds ratios (ORs) and 95% confidence intervals (CIs). Subgroup analyses were performed according to race. Qualitative systematic review was conducted for variants that were studied in only one study. All analyses were performed using STATA 12.0. Results: 13 studies investigating the associations between polymorphisms in five inflammatory cytokine genes (TNF-α, IL-1α, IL-1β, IL6, and IL-12B) and IA were reviewed. Combined results showed that the A allele of TNF-α rs1800629 polymorphism has a protective effect against IA (dominant model: OR=0.65, 95% CI=0.47–0.89, p=0.007). No associations were identified between polymorphisms in IL-1α rs1800587, IL-1β rs16944, IL6 rs1800795 and rs1800796, or IL-12B rs3212227 and IA risk. Conclusion: This review demonstrated an association between TNF-α rs1800629 polymorphism and IA in Caucasians, illustrating the potentially important role of genes involved in inflammation in IA.

Special issue of biomedical engineering letters on advances in intelligent prostheses

Liming Salvino,Wing Kong Chiu,Jerome Lynch,Kenneth J. Loh 대한의용생체공학회 2020 Biomedical Engineering Letters (BMEL) Vol.10 No.1

This Special Issue comprises original and review papersfrom engineering and medical professionals expounding onthe state of the art in engineering and the physical sciencesthat aim to enhance the performance of prosthetic devicesincluding socket prostheses and osseointegrated prostheses. Advances in physical science disciplines are poised torevolutionize how doctors and patients view and use prostheses. With monitoring and control capabilities, advancedprostheses can provide functionalities far beyond simplelimb extension and load support. Prostheses with built-insensing, embedded intelligence such as real-time monitoringand infection eradication, self-actuation, and control havebeen quickly advancing over the past 10 years or so. Giventhe rapid emergence of these transformative developments,this Special Collection is designed to collate a volume ofwork devoted to cutting-edge developments in advancesin intelligent prostheses that enhance medical care and thequality of life of patients with limb loss. Additionally, theseprosthetic functionalities and technologies may be utilized as“special wearables” to collect and mine human physiologicaldata in order to gain knowledge in fundamental scienceson neurological and biomechanical eff ects of injury andrehabilitation.

Privacy-Preserving, Energy-Saving Data Aggregation Scheme in Wireless Sensor Networks

Liming Zhou,Yingzi Shan 한국정보처리학회 2020 Journal of information processing systems Vol.16 No.1

Because sensor nodes have limited resources in wireless sensor networks, data aggregation can efficientlyreduce communication overhead and extend the network lifetime. Although many existing methods areparticularly useful for data aggregation applications, they incur unbalanced communication cost and waste lotsof sensors’ energy. In this paper, we propose a privacy-preserving, energy-saving data aggregation scheme(EBPP). Our method can efficiently reduce the communication cost and provide privacy preservation to protectuseful information. Meanwhile, the balanced energy of the nodes can extend the network lifetime in our scheme. Through many simulation experiments, we use several performance criteria to evaluate the method. Accordingto the simulation and analysis results, this method can more effectively balance energy dissipation and provideprivacy preservation compared to the existing schemes.

Agetolitids (Upper Ordovician; South China) 산호의 분포

Liming Guan,우주선,박진오,이동찬,이동진 한국고생물학회 2009 정기총회 및 학술발표회 Vol.2009 No.-

Effects of Different Ti Content in Zn-30Al Filler Metals on the Microstructure and Mechanical Properties of Mg-Al Butt Joints

Liming Liu,Fei Liu,Disheng Fang,Hongyang Wang 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

Based on the idea of alloying welding seam, a series of Zn-30Al-xTi filler metal was used to join Mg/Al dissimilar metals by tungsten inert gas welding, where the x was 0, 0.05, 0.1, 0.5, and 1, respectively. The effect of various Ti content on the microstructure and the mechanical property of Mg-Al butt joints was investigated. Results indicated that the fusion zones of the joints were mainly composed of α-Al, MgZn2 and Al-Zn eutectics when the Ti content was 0.05, 0.1 and 0.5 wt.%, and Al3Ti compounds could precipitate first and act as nucleuses during solidification process when the Ti content was appropriate. The mechanical property of the weld joints improved consistently to the increasing content of Ti. The optimum average tensile strength of the joints reached 148 MPa when Zn-30Al-0.5Ti was used, but the average tensile strength decreased again when Zn-30Al-1Ti was used.