Functional Properties of Permeate Fractions Obtained from Bastard Halibut Roe Extracts by Ultrafiltration and It’s Biological Activities

Gyoon-Woo Lee1,In Seong Yoon,Su Gwang Lee,Jung-Suck Lee,Young-Mi Kang,Jin-Soo Kim,Min Soo Heu 한국수산해양기술학회(구 한국어업기술학회) 2016 한국수산해양기술학회 학술발표대회 Vol.2016 No.10

肺臟切除가 反對側 肺臟 第Ⅱ型 肺胞細胞의 機能에 미치는 影響

李錫江,朴載植,金亨鎭 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

一側 肺切除時 反對側 肺臟이 代償性 增殖을 한다는 것은 周知의 事實이다. 特히 代償性 增殖時 肺胞表面活性物質을 分泌하는 것으로 알려진 第Ⅱ型 肺胞細胞의 機能은 어떤 樣相을 나타내는지 究明하기 위하여 試圍한 本 硏究 結果는 다음과 같다. 肺洗滌液의 燐脂質 燐量은 對照群에서 8.04±0.74μMol/gm wet lung wt.인데 比해 肺切除後 第 1,2 및 3일에는 有意하게 減少하였으나 第 7일에는 6.91±0.57μMo1/gm wet lung wt.로서 第3일에 比해서 增加하는 傾向을 보였다. 肺洗滌液의 蛋白量은 對照群이 5.18±0.15㎎/gm wt lung wt.인데 比해 肝切除後 第 1 및 2일에는 各各 7.90±0.68 및 7.07±0.75㎎/gm wet lung wt.로 有意하게 增加를 보였으며 第 3 및 7일에는 對照群과 비슷하였다. 對照群의 右側肺臟 무게는 3.26±0.12gm인데 比해 肺切除後 第 1,2 및 3일에는 別 變化가 없었으나 第 7일에는 3.86±0.2gm으로 對照群에 比해 有意하게 增加하였다. 15㎖ 및 30㎖의 肺容積에 대한 肺內壓 測定은 動的 및 靜的肺內壓이 모두 肺切除後 第 1 및 2일에 上昇하는 傾向을 보였고 第 3및 7일에는 減少하여 對照群과 비슷하거나 낮았다. 兩側 副腎무게는 對照群이 230.2±19.5㎎인데 比해 肺切除後 第 1,2 및 3일에는 別 變化가 없었으나 第 7일에는 336.7±39.2㎎으로 對照群에 比해 有意한 增加를 하였다. 體重은 對照群에서 1.70㎏인데 比해 肺切除後 第 1,2 및 3일에 다같이 減少하였으며 第 7일에는 對照群의 그것과 비슷하였다. It is generally accepted that conpensatory lung growth occurs in the remaining tissue after pneumonectomy; the unilateral pneumonectomy causes the weights, number of alveoli, and alveolar cells to increase to the level of total paired lung, including alveolar type Ⅱ cell which secretes a lipoprotein mixture called surfactant. In order to study the effects of unilateral pneumonectomy on the function of type Ⅱ cell in the contralateral lung during compensatory period, left pneumonectomy was performed on rabbits (1.5-1.8kg body weight), and phospholipid phosphorus and protein contents in the right lung lavages, the weight of the right lung, intrapulmonic pressures at 15 and 30㎖ lung volumes, and bilateral adrenal weights were studied on the 1st, 2nd, 3rd and 7th postpneumonectomy day. The right lung was lavaged with isotonic saline and phospholipid phosphorus in the lung lavage was determined by the modified method of Beveridge and Johnson, and protein was determined by the method of Lowry et al. The results obtained are summarized as follows. Phospholipid phosphorus contents in the control was 8.04±0.74μMol/gm wet lung wt, but a significant decrease on the 1st, 2nd and 3rd postpneumonectomy day was observed compared with the control. However, phospholipid phosphorus on the 7th postpneumonectomy day was increased to 6.91±0.57μMol/gm wet lung wt comparing with the 1st, 2nd and 3rd postpneumonectomy day. The protein contents of the lung lavage was increased on the 1st and 2nd postpneumonectomy day but decreased to the control level on the 7th postpneumonectomy day. The lung weight on the 1st, 2nd and 3rd postpneumonectomy day was not different from the control but increased significantly to 3.86±0.25gm on the 7th postpneumonectomy day compared with the control value of 3.26±0.12gm. The static and dynamic intrapulmonic pressures were increased on the 1st and 2nd postpneumonectomy day but decreased to the control value on the 3rd and 7th postpneumonectomy day. The bilateral adrenal weight on the 1st, 2nd and 3rd postpneumonectomy day was not different from the control but increased significantly to 336.7±39.2mg on the 7th postpneumonectomy day compared with the control value of 230.2±19.5mg. The body weight in the control was 1.70±0.05kg but a decreased value was observed on the 1st, 2nd and 3rd postpneumonectomy day followed by a return to the control on the 7th postpneumonectomy day.

액체-고체 순환유동층의 상승관에서 액상의 점도가 고체체류량 및 열전달계수에 미치는 영향

이찬기,강석환,신광식,송평섭,강용,김상돈 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.4

직경이 0.102 m이고 높이가 3.5 m인 액체-고체 순환유동층에서 액체의 점도가 고체입자의 체류량과 열전달계수에 미치는 영향을 검토하였다. 밀도가 2,500 kg/㎥인 유리구슬(d_(p)=1.0, 1.7, 2.1 또는 3.0 mm)과 CMC 용액(μ_(L)=0.96-38 mPas)을 각각 고체상과 액체상으로 사용하였다. 액체-고체 순환유동층에서 고체입자의 체류량은 액체의 유속이나 점도가 증가함에 따라 감소하였고 유동입자의 크기나 입자의 순환속도가 증가함에 따라 증가하였다. 액체-고체 순환유동층에서 열전달계수는 액체의 점도가 증가함에 따라 감소하였고 유동입자의 크기나 순환속도가 증가함에 따라 증가하였으나, 액체의 유속이 증가함에 따라서는 큰 변화가 없었다. 액체-고체 순환유동층에서 고체입자의 체류량과 열전달계수는 다음과 같이 실험변수와 무차원군에 의해 상관식으로 얻을 수 있었다. E_(S) = 0.783U^(-0.100)_(L)G^(0.164)_(S)d^(0.302)_(p)μ^(-0.036)_(L) h = 4349.3U^(0.010)_(L)G^(0.099)_(S)d^(0.151)_(p)μ^(-0.015)_(L) ◁그림삽입▷ Effects of liquid viscosity on the solid holdup and heat transfer coefficient have been investigated in the riser of a liquid-solid circulating fluidized beds, whose diameter is 0.102 m and 3.5 m in height. Glass beads (d_(p)=1.0, 1.7, 2.1 or 3.0 ㎜) whose density is 2,500 ㎏/㎥ and aqueous solutions of carboxymethy cellulose (CMC)(μ_(L)=0.96-38 mPas) have been used as the solid and liquid phase, respectively. It has been found that the solid holdup decreases with increasing liquid velocity or viscosity, but it increases with increasing solid particle size or solid circulation rate. The heat transfer coefficient decreases with increasing liquid viscosity but it increases with increasing particle size or solid circulation rate, however, it does not change considerablely with increasing liquid velocity. The solid holdup and heat transfer coefficient have been well correlated in terms of operating variables or dimensionless groups as fellow: ◁수식원문참조▷

구성주의 프로그램에서의 수교육 활동에 관한 연구

강숙현,이석순 광주보건대학 1989 論文集 Vol.14 No.-

The purpose of this study was to search the philosophical, theoretical back-grounds and practices in the Number Activities of the Constructivist Early Childhood Education Program. In order to achieve this purpose, following questions of study was given. 1. What are the philosophical, theoretical backgrounds in the Constructivist Program ? 2. What are the contents and unfolding methods i n the Constructivist Program ? 3. How emphasize the motives for number activities ? 4. How are the child's grouping in the number activities 5. How compose the social environment in the Consructivist Program ? 6. What are the teacher's role and teaching strategies To summarize, the philosophical, theoretical backgrounds ; contents ; unfolding methods ; motivation ; grouping of children ; and the teacher's role and teaching strategies of Constructivist Early Childhood Program, present study referring the books, dissertations. program brochures and seminar informations about the Constructivism. The conclusions of this study are as follows ; 1. In the philosophical, theoretical backgrounds, Constructructivist progam is based on Piaget's cognitive-developmental theory. It was developed by Constance Kamii and Rheta DeVries in 1967. 2 The aims of the Number Activities of Constructivist are 'DEVELOPMENT" and "INTELLECTUAL AUTONOMY".. Constructivist includes activities through the everyday life and group games. Therefore the Constructivist program need not special materials. But situations within everyday life and are emphasized so as to develop to construct number's structure and relationships. 3. The Number Activities of Constructivist Early Childhood Education Program emphasize children's interest and motivation. Children's interest become the motives for spontaneous activities . 4. Children are grouped by themselves because Constructivist program emphasize children's interest and spontaneous participation. 5. In Constructivist program, the social environment are emphasized inter-action. Active interaction involves the conflict and the cooperation. 6. Constructivist teachter observes and takes part in play activities, and stimulus children's intellectual thinking. And the teacher arranges the educational environments, mainly use indirect method, i.e. children's everyday life and group games in practical teaching-learning situation.

항암성 천연물 및 그 유사체 (X) : L1210 및 S-180에 대한 하늘타리의 항암성 Antitumor Activity of Trichosanthes kirillowii on L1210 and S-180 Tumors

이유희,강석균,안병준 충남대학교 약학대학 의약품개발연구소 1986 藥學論文集 Vol.2 No.-

A strongly cytotoxic fraction (Fc-2, ED_50=0.0003㎍/㎖) against L1210 cell was obtained from the root, seed and fruit of Trichosanthes kirilowii, Administration of Fc-2 prolonged the life span of the BDF1 mice bearing L1210 and the ICR mice bearing S-180 by 135% and 130%, respectively. The Fc-2 affected L1210 cells were enlarged in their diameters two or three times in comparison with the untreated ones. When 20㎎/㎏ of Fc-2 was administered, intraperitoneally, all the mice were killed.

운동부하 흰쥐에서 포도당 증합체가 심근 당원에 미치는 영향

이석강,조성현,김용운,박덕일,김종연,장용찬 대한스포츠의학회 1994 대한스포츠의학회지 Vol.12 No.1

운동부하전 섭취한 탄수화물이 운동흰쥐의 심근 및 골격근이 당원량에 미치는 영향을 측정하여 심근과 골격근의 대사적 차이점의 일단을 연구한 본 논문의 결과는 다음과 같다. 심근과 골격근 당원량(mg/ g wet tissue, 이하 단위 동일)은 정상군에서 심근 2.62±0.51로서 골격근의 3.37±0.58보다 낮았다(p<0.01). 심근 당원량은 정상군이 2.62±0.51이었다. 운동 30분에서 대조군은 정상군의 63%수준으로 감소하였으며(p<0.01), 탄수화물섭취군은 정상군의 86 및 90%수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 대조군의 142 및 137%정도로 양군 모두 대조군보다 유의하게(p<0.01) 높았다. 운동 60분에는 대조군은 운동30분의 78%(정상군의 49%)수준으로 감소하였으며(p<0.05) 탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 운동 30분에 비하여 72(p<0.01, 정상군의 62%), 81%정도(p<0.05, 정상군의 73%)로 감소하였다. 포도당 섭취군의 당원량은 대조군의 147% 정도로 대조군보다 유의하게(p<0.01) 높았다. 운동 120분에서 대조군은 운동 60분에 비하여 63%(정상군의 33%) 수준으로 감소하였으며 (p<0.01) 포도당섭취군은 운동 60분의 69%(정상군의 50%)수준으로 유의하게 감소하였으나, 포도당 중합체 섭취군은 차이가 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 202 및 162% 정도로 양군 모두 대조군보다 유의하게(p<0.01)높았다. 골격근 당원량은 정상군이 3.37±0.58 이었다. 운동 30분에서 대조군은 정상군의 69% 수준으로 감소하였으며(p<0.01) 탄수화물섭취군은 포도당 중합체 및 포도당섭취군에서 각각 정상군의 85 및 88% 수준으로 감소하였으나 통계적으로 유의한 차이는 없었다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군의 125 및 129% 정도로 양군 모두 대조군보다 유의하게(p<0.05) 높았다. 운동 60분에는 대조군은 운동 30분의 77%(정상군의 53%) 수준으로 감소하였으며(p<0.05)탄수화물섭취군은 포도당중합체 및 포도당섭취군에서 각각 30분에 비하여 93(정상군의 80%), 89(정상군의 78%) %정도로 감소하였으나 통계적으로 유의하지는 않았다. 포도당중합체 및 포도당 섭취군의 당원량은 각각 대조군의 152 및 149% 정도로 대조군보다 유의하게 (p<0.001) 많았다. 운동 120분에서 대조군은 운동 60분에 비하여 50%(정상군의 26%) 수준으로 감소하였으며(p<0.001) 포도당 섭취군은 운동 60분의 75%(정상군의 58%)수준으로 유의하게(p<0.05) 감소하였으나 포도당 중합체 섭취군은 대조군의 84%(정상군의 67%)정도로 감소하는 경향을 보였다. 탄수화물 섭취군의 당원량은 포도당 중합체 및 포도당섭취군에서 각각 대조군우ㅢ 255 및 221% 정도로 양군 모두 대조군보다 유의하게(p<0.001) 높았다. 이상의 결과로 미루어 보아 심근과 골격근 간의 생리, 생화학적 특성의 차이에도 불구하고 탄수화물의 섭취후 중등도 정도의 달리기 운동시 근 당원의 함량에 미치는 영향은 정도의 차이는 있으나 같은 경향을 나타내었으며, 골격근에서 심근보다 더 많은 근 당원 절감효과를 나타내었다. 또한 운동전 탄수화물의 섭취시 이러한 근 당원 절감 효과로 장시간의 운동시 심근 및 골격근 당원의 고갈 지연으로 운동수행능력의 증가가 기대되며, 특히 포도당 보다는 포도당 중합체의 섭취시 효과가 더 크리라고 사료된다. The purpose of this study was to investigate the effect of glucose and glucose plymer ingestion on the concentration of the cardiac muscle glycogen at 30, 60 and 12 minutes of the treadmill running in rats. Rats were randomly assinged to normal, control, and carbohydrate groups. Carbohydrate group was divided into glucose, and glucose polymer ingested rats. The experiment was carried out after overnight fasting. Rats were administrated either water (control) of carbohydrates of formulated glucose(6%), and glucose polymer(6%) via polyethylene tube to the stomaach before treadmill run. Exercise was loaded by a treadmill run at the speed of I ㎞/hr for 30, 60, and 120 minutes. The results of experiment were as follows: Glycogen content of the cardiac muscle was lower than that of the skeletal muscle (plantaris) in normal rats. Glycogen content of the cardiac muscle was decreased in proportion to the exercise time compared to normal in control and carbobydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in decreasing order at 120 minutes treadmill running (% change of glycogen content of control, glucose, and glucose polymer compared to normal rats were 33, 50, and 63% in the cardiac muscle at 120 minutes of treadmill running respectively). Glycogen content of the skeletal muscle was decreased in proportion to the exercise time compared to normal in control and corbohydrates ingested rats. It decreased in control, glucose, and glucose polymer compared to normal rats in ecreasing order 120 minutes treadmill running(% change of glycogen content of control, glucose, and glucose polymer compared to normal were 26, 58, and 67% in the cardiac muscle at 120 minutes of treadmill running respectively). Change of the glycogen content of the cardiac and the skeletal muscle during treadmill running after glucose and glucose polymer ingestion showed that both muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrates ingested rats. Decreasing rate of glycogen content of cardiac and skeletal muscles during treadmill running compared to normal in carbohydrates ingested rats was lower than that of control. From these results, it is suggested glycogen content of cardiac and skeletal muscles had tendency to decrease in proportion to exercise time similarly in control and carbohydrate ingested rats. Glycogen content of cardiac and skeletal muscles in carbohydrate singested rats less decreased than that of control during treadmill running compared to normal. Glucose polymer of the carbohydrates ingested rats in considered as the most effective for glycogen sparing according to exercise time in both muscles. Therefore, intake of carbohydrate, especially glucose polymer, before exercise may be increase performance of the prolonged moderate exercise.

한국에 있어서의 알레르기성 호흡기질환의 기인성 진균 : A Clinical Study on the Offending Fungal Allergen of Allergic Respiratiory Diseases in Korea

姜?榮,李重根 中央醫學社 1977 中央醫學 Vol.33 No.6

The offending fungal allergens in six hundred twelve cases of korean patients (male 344 cases, female 268 cases) with allergic respiratory diseases such as bronchial asthma and allergic rhinitis were analyzed by the intradermal skirt test with diagnostic standard allergen extracts. The incidence of positive skin test in allergic respiratory diseases were 67.6 % : 66.7% in bronchial asthma, 67.8% in allergic rhinitis and 89. 5% in combined. Cases. In allergic respiratory diseases, the incidence of positive skin tests against one species of fungus was 50.5%, 42.5% against 2^-3 species, 5.8% against 4?6 species, and 1.2%' against 7?9 species respectively. In allergic respiratory diseases, the incidence of the positive skin reactions to fungal extracts were: 27.1% against Candida albicans, 19. 3% against Trichophyton species, 8.7% against Rhizopus nigricans and 7.5% against Fusarium species.

벼의 혹명나방의 성 유인 물질의 합성과 생물활성시험

강석구,박정민,이정운,고현관 成均館大學校 科學技術硏究所 1983 論文集 Vol.34 No.2

벼의 혹명나방의 성 유인 물질인 (Z)-13-옥타데센-1-올 아세테이트를 새로운 방법으로 합성하였다. 즉, 1-헥신의 리튬 음이온을 보호된 12-브로모-1-도데칸을 THP 에테르와 반응시켜, 13-옥타데신-1-올 THP에테르를 만든후 Lindlar 촉매에 의한 수소화 반응을 시켜 (Z)-13-옥타데센-1-올 THP에테르를 얻는다. 산촉매하에서 보호기를 제거한 후 피리딘 용매하에서 무수아세트산을 반응시켜 최종 생성물인 (Z)-13-옥타데센-1-올 아세테이트를 좋은 수율로 얻었다. 농촌진흥청의 곤충과와 공동으로 상기에서 합성된 성 유인 물질을 이용하여 혹명 나방의 숫컷에 대한 성 유인 효과를 실험해본 결과 성 유인 작용이 있는 것으로 나타났다. A new method is described for the synthesis of (Z)-13-octadecen-1-ol acetate(1), the sex pheromone of the Rice Leaf Folder moth. Lithium acetylide of 1-hexyne was alkylated with 12-bromo-1-dodecanol THP ether. 13-Octadecyn-1-ol THP ether was stereoselectively reduced over Lindlar catalyst(Pd/BaSO_4) to give (Z)-13-octadecen-1-ol THP ether. (Z)-13-octadecen-1-ol THP ether was deprotected and acetylated with acetic anhydride and pyridine to afford the final product, (Z)-13-octadecen-1-ol acetate. Biological activity test of the synthetic compound, (Z)-13-octadecen-1-ol acetate was tested in the field using polyethylene capsules as containers. The numbers of moths trapped with pheromone vials were counted.

흰쥐에서 연령이 골격근의 당 대사에 미치는 영향

정응찬,우운기,이석강 영남대학교 기초/임상의학연구소 2001 Yeungnam University Journal of Medicine Vol.18 No.1

Background: It is doubtful that aging causes deteriorated glucose metabolism and insulin resistance of skeletal muscle. Some researchers had different results about it. So we have studied the mechanism responsible for the abnormal glucose tolerance associated with aging in rapidly growing and matured rats. Materials and Methods: Animals were used S.D. rats. Growing rats were 7 weeks old (BW: 160-190 gm) and matured rats were 28 weeks old (BW: 420-525 gm). Results: Fasting blood glucose and plasma insulin levels were significantly elevated in matured rat compared with growing rats. And during oral glucose tolerance test the glucose level was also significantly elevated in matured rats. These results confirmed an insulin resistant state of aging. Insulin levels at 30 minutes of oral glucose tolerance test was significantly elevated in growing rat. But at 120 minutes it was maintained at higher level in matured rats than in growing rats. It suggested the possibility of increased insulin secretion by initial stimulation of beta-cells in growing rats, and increased secretion and decreased catabolic rate of insulin in matured rats. Glucose uptake rate of soleus muscle in matured rats was lower than that of growing rats, but the difference was not statistically significant. The dose(insulin)-responsive(glucose uptake) curve of soleus muscle was only slightly deviated to the right side. Conclusion: Glucose metabolism of rat skeletal muscle was worsened by aging. The data of glucose uptake experiments suggested the possibility of insulin resistance of skeletal muscle in matured rats, but the mechanism of insulin resistance of skeletal muscle need further studies.

씨름선수에서 체중 감량과 회복기의 Leptin과 PAI-1 변화

김용운,도경오,권태동,박덕일,장응찬,박소영,김종연,이석강 대한스포츠의학회 2000 대한스포츠의학회지 Vol.18 No.2

Obesity which is defined as accumulation of excess body fat, is central factor of insulin resistance syndrome. Recently, it is revealed tat adipose tissue is not simply an energy storage organ but it also secretes a variety of molecules which affect the metabolism of the whole body, those are leptin and plasminogen activator inhibitor-1(PAI-1). Therefore, leptin and PAI-1 are increased in the obese state. Leptin regulates energy homeostasis and satiety and PAI-1 regulates fibrinolytic system. For these reasons, elevated levels of leptin and PAI-1 are considered as link factors between obesity and insulin resistance syndrome. However, the exact regulating mechanism for serum levels of leptin and PAI-1 is not fully understood yet. In this study, to evaluate the regulating mechanisms of serum levels of leptin and PAI-1 according to the weight changes, we measured leptin, PAI-1, physical, metabolic, and endocrine parameters during 10 days of weight reduction and 10 days of regain period in 7 young athletes. The mean body weight change was -4.7 kg(5.0%) in the weight reduction period and -2.4 kg(2.5%) in the weight regain period compared to baseline value. Baseline level of leptin in athletes was 1.7±0.66 ng/ml, which was significantly correlated with body weight, BMI, percent body fat, body fat mass, triglyceride, insulin, and PAI-1. Baseline level of PAI-1 in athletes was 16.6±5.26 ng/ml, which was significantly correlated with body weight, BMI, triglyceride, insulin, and leptin. Leptin was decreased to 0.7±0.39(44% of the basaline value) in the weight reduction period, and increased to 1.9±0.64(119% of the baseline value) in the regain period. PAI-1 was decreased to 7.4±2.72(44% of the basaline value) in the weight reduction period, and increased to 22.8±7.33(138% of the baseline value) in the regain period. The changes of leptin during weight reduction period were significantly correlated with the changes of insulin(r=0.890, P<0.01) and triglycerides(r=0.874, P<0.01). The changes of PAI-1 during weight reduction period were significantly correlated with the changes of FFA(r=0.889, P<0.01) and triglycerides(r=0.869, P<0.05). The changes of both leptin and AAI-1 during weight regain period were significantly correlated with the changes of insulin(r=0.755 and 0.849, P<0.05, respectively). In summary, these results suggest that serum levels of leptin and PAI-1 were affected by weight cycling, the percentages of change were more greater than that of weight change, and rebound phenomena were occurred during weight regain period.