Production and Characterization of a Thermo-alkalotolerant Cyclodextrin Glucanotransferase from Thermo-alkalophilic Bacillus cereus B-13

Seo, Seung-Bo,Kim, Jae-Ho,Lee, Dae-Hyoung,Lee, Jong-Soo 배재대학교 자연과학연구소 2005 自然科學論文集 Vol.16 No.1

내열성의 cyclomaltodextrin glucanotransferase (CGTase)를 이용하여 열에 안정한 싸이클린 텍스트린 (CD)을 생산하기 위하여 매우 높은 CGTase 활성을 보이는 고열성이며 호알카리성인 B-13 세균을 분리하여 형태적, 생리학적 특성과 16S 리보솜 RNA 서열분석 등을 통하여 Bacillus cereus B-13으로 동정하였다. Bacillus cereus B-13을 2% 가용성 전분, 1% 효모 추출물, 1% Na_(2)CO_(3) 등을 함유하는 SYC 배지 (pH 8.5)에 접종하여 50℃에서 24시간 배양하였을 때 최고의 CGTase 활성(130 U/ml)을 보였다. 또한 부분 정제된 CGTase의 작용 최적 온도와 pH는 각각 65℃, pH 8.5-9.0 이었고 80℃이하와 pH 5.0-10.0에서 안정하였다. 1% 가용성 전분을 부분 정제한 CGTase와 작용 시켰을 때 49%의 CD 수율을 보였다. To produce a thermostable cyclodextrin by using thermotolerant cyclomaltodextrin glucanotransferase(CGTase), a thermophilic and alkalophilic bacterium isolate, designated B-13 showing the highest CGTase activity was isolated from natural sources and identified as Bacillus cereus B-13 based on the morphological and physiological characteristics, and 16S rRNA sequence. The maximal CGTase activity (130 U/ml) was obtained when Bacillus cereus B-13 was cultured in SYC medium containing 2.0% soluble starch, 1.0% yeast extracts, 1% corn steep liquor and 1% Na_(2)CO_(3) (pH 8.5) at 50℃ for 24 h and about 80% of maximal activity was also showed in the culture broth of 60℃ for 18 h . Optimum reaction temperature and pH of the partial purified CGTase for soluble starch were 65℃ and pH 8.5-9.0, respectively. The partial purified CGTase were also stable below 80℃ and pH 5.0-10.0. When 1% soluble starch was digested with the partial purified CGTase, the yield of cyclodextrin was 49%.

만성 승모판 패쇄부전증 환자에서의 안지오텐신 전환효소 억제제의 장기 투여 효과

김대희,이명묵,이해영,조현재,박승정,서재빈,서정원,양한모,윤창환,조상호,이준희,김용진,김명아,손대원,오병희,박영배 대한순환기학회 2004 Korean Circulation Journal Vol.34 No.2

참동계고이의 편찬과 구조

이대승(Lee Dae-seung) 한림대학교 태동고전연구소 2013 泰東古典硏究 Vol.30 No.-

본 글은 주희의 참동계고이에 대한 연구이다. 본 글에서는 심도 있는 참 동계고이 연구를 위한 토대작업으로서 참동계고이의 집필시기와 편찬과 정, 참동계고이의 판본, 그리고 그 구조를 다루었다. 주희는 1186년(57세) 즈음부터 참동계에 이미 관심을 갖고 있었다. 그는 채원정과 함께 1196년부터 집필 작업을 시작하여, 1197년 말엽과 1198년 초엽 사이에 채원정의 아들 채연으로 하여금 1차 간행본을 출판케 한다. 그리고 주희는 이후 수정작업을 거쳐 1199년 봄에 정본을 간행한다. 주희 당시 간행된 채연본은 현재 통행되지 않으며, 현행본은 원대 황서절이 편집한 것으로서 경문과 주석의 문자에서 채연본과 약간의 차이가 있는 것으로 보인다. 주희의 참동계고이는 팽효의 참동계분장통진의를 바탕으로 다른 참동계 판본 들과의 비교·대조 작업을 거쳐 저술된 것으로, 그 구조는 다른 참동계 판본과 큰 틀에서 유사하다. This thesis is a foundation research for an in-depth study for Chantong qi kaoyi (參同契考異) of Zhu Xi and aims to examine the publication and structure of Chantong qi kaoyi. Zhu Xi was already interested in Zhouyi cantong qi (周易參同契) by 1186, his 57 years of age. He started to write Chantong qi kaoyi through a cooperation with Cai Yuanding (蔡元定) in 1196 and made Cai Yuan (蔡淵) publish the first version of Chantong qi kaoyi between end of 1197 and beginning of 1198. He published the formal edition of Chantong qi kaoyi in the spring of 1199 through an amending work with Cai Yuan. The edition of Cai Yuan does not pass on these days, and the current edition is the edition of Huang Ruijie (黃瑞節) on Yuan (元) dynasty, which is a little bit different with the edition of Cai Yuan. Chantong qi kaoyi was made by the comparative study with other Zhouyi cantong qi editions on the basis of Cantong qi fenzhang tong zhenyi (參同契分章通眞義) of Peng Xiao (彭曉). The structure of Chantong qi kaoyi is generally similar with the other editions of Zhouyi cantong qi.

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

악성 림프종 환자에서 발생한 다형홍반

여운철,조광현,이유신,허대석,이승철 ( Un Cheol Yeo,Kwang Hyun Cho,Yoo Shin Lee,Dae Seog Heo,Seung Chul Lee ) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

A Case of Erythema Multiforme A ssociated with Malignant Lymphoma Un Cheol Yeo, M.D., Kwang Hyun Cho, M.D., Yoo Shin Lee, M.D., Dae Seog Heo*, M.D., Seoung Chul Lee**, M.D Department of Dermatology and Internal Medicine*, College of Medicine, Seoul National University, Seoul, Korea, Department of Dermatology**, College of Medicine, Inha University, Kyung Gi Do, Korea We report a case of erythema multiforme associated with malignant lymphoma. Fifty-six-year-old male patient visited our clinic complaining generalized skin lesions which were compatible with erythema multiforme clinically and histopathologically. The skin lesions were treatment-resistant, recurrent and persistent for 3 years. In consideration of old age, atypical features of erythema multiforme and no other demonstrable etiology of erythema multiforme, we studied for internal malignancy. Malignant lymphoma at the duodenum was revealed by UGI series, abdominal CT, and endoscopic biopsy. After chemotherapy, the tumor mass and active skin lesions were cleared simultaneously. Erythema multiforme may be regarded as one of the paraneoplastic syndromes.

방과후 학교의 운영체제 개선 방안 연구

이승미,김대영,홍후조,민부자 한국교육과정학회 2007 교육과정연구 Vol.25 No.3

RC 프레임면내 조적채움벽의 내진성능 평가에 관한 실험적 연구

이정한,차승렬,강대언,오상훈,이용재,이원호,김상대 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1(구조계)

The objectives of this study are 1) to find out the shear behavior and shear capacity of RC bare frames, brick-infilled RC frames, 2) to evaluate the equation of the shear capacity of two types of RC frames by comparison the Japanese Standard and modified equation adopting the test results. The main variable is the absence of brick infill wall. The test results show that the shear capacity of specimen IF-4.2 is 2.3 times as high as that of the specimen IF-4.2.The shear behavior of RC bare frame which is governed by predominant flexural yielding is changed to predominant shear yielding by affection of the brick infill wall. A comparison of the results obtained by the Japanese Standard equation and the modified equation to the test data indicates that the proposed equation is compatible with the test results.

한국판 스미스클라인 비챰의 '삶의 질'척도를 이용한 공황장애 환자의 삶의 질에 관한 연구

이승호,제영묘,김상엽,이대수,최은영 대한생물치료정신의학회 2000 생물치료정신의학 Vol.6 No.1

Objectives : In this study, we researched the change of 'quality of life(QOL)' of patient with panic disorder by KvSBQOL and the predictors affecting the change of 'quality of life'. Methods : 30 outpatient with panic disorder were tested by questionnare for panic disorder and KvSBQOL. Frst, they were classified as when healthy before panic episodes(a point of time 1), when first diagnosed as panic disorder in the psychiatry(a point of time 2), and when treated after diagnosis(a point of time 3). Second, the change of QOL scores in five factors at each point was observed, Third, the correlation between the change of QOL scores(point 3 score-point 2 score) and the several predictors(Onset age, Degree of change in panic symtom, Degree of change in anticipatory anxiety, Degree of change in agraphobia, Spended time to diagnose panic disorder, Duration of treatment) were studied. Results : 1) QOL mean scores of a point of time 1,2 and 3 were 166.267±38.24, 94.367±34.00, 161.367±34.37, respectively. 2) The change of QOL scores in all five factors of KvSBQOL(factor1 Competence, factor2 Psychological well-being, factor3 Stability, factor4 Physical well-being, factor5 Activity)at each point was significant(P<.000). 3) The change of QOL mean scores(point 3 score-point 2 score) have significantly negative correlation with the change of score in anticipatory anxiety but not other predictors. Conclusions : Difference in QOL scores between a point of time 1 and 3 is 4.9, indicating that the psychiatric treatment is important in improvement of QOL, the anticipatory anxiety is an important predictor of QOL(P<.001). and it suggested that diminished anticipatory anxiety is very important to maintain of high QOL in patient with panic disorder.

본딩 와이어의 특성 파라미터 추출에 관한 연구

이승대 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.1

The characteristics of a bonding wire which is widely used in interconnection bare chip or bare die to the substrate is studied. The ohmic resistance is calculated using the phenomenological loss equivalence(PEM) method. In addition, input impedance according to the structure of bonding wire is obtained using the method of moment. The inductance, the capacitance and the characteristics parameters of a bonding pad interconnection bonding wire are calculated.

[논문]스트렷-타이 모델에 의한 사각형 단면 코벨의 설계 기법

김대성,김태완,이승훈,엄장섭,진치섭 釜山大學校生産技術硏究所 2004 生産技術硏究所論文集 Vol.63 No.-

콘크리트 코벨은 D-영역을 갖는 대표적인 구조물로서 복잡하고 다양한 형상의 파괴메커니즘을 보인다. 이는 응력집중과 기하학적인 불연속으로 일반적인 보 이론의 적용이 불가능하여 해석 및 설계에 어려움이 많다. 본 연구에서는 지압파괴,압축파괴 및 전단파괴를 유발하는 복잡한 파괴메커니즘을 형성하고 있는 사각형 단면 코벨의 거동 및 파괴형상을 예측하고 스트릿-타이 모텔을 적용하여 해석 및 설계를 하였다. 또한 현행의 설계방법과의 비교를 통하여 스트렷-타이 모델이 보다 합리적이고 경제적인 설계방법임을 보였다.