카드뮴의 중추신경계 독성유발 기전

이종화,장봉기,박종안,박종영,김완종,우기민 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.3

Although numerous studies have shown that cadmium disturbs the normal biological processes in central nervous system the mechanism of toxicity is not well understood The present study has investigated the effect of cadmium on oxidative stress, Na+/K- ATPase activity and the aggregation of amyloid beta peptide(β-amylotd) in neuronal cell Ime, HT22 cell LC_(5) and LC_(50) of cadmium for HT22 cell resulted from MTT assay was 4 1 μM and 9 5 μM, respectively Cadmium(2 to 8 μM) dose-dependently increased the lipid peroxidation and decreased the content of glutathione Cadmium 4 μM showed a significant decrease in Na^(-)/K^(+) ATPase activity as compared with control group The aggregation of P-amyloid was accelerated in a dose-dependent manner by the treatment with 2 to 8 μM cadmium These results suggest that the neurotoxicity of cadmium can be mediated by the increase in oxidative stress and decrease in Na^(-)/K^(+) ATPase activity.

톨루엔을 포함한 유기용제의 직업적 폭로로 인한 신경내분비계 영향

이채언,이종태,정의화,손혜숙,문덕환,전진호,강정학,이창희,김휘동,김종한,정귀옥 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2

Long term occupational exposure to solvent mixtures may cause adverse effects to the central nervous system with neurobehavioral symptoms. And some organic slovents have been suggested to cause impairment of tuberoinfundibular dopaminergic activity and neurochemical mechanisms controlling pituitary secretion. For the purpose of assessing neuroendocrine effects in occupational solvents exposure, hormonal study settings (shoes-manufacturing industry & fishing products industry) and compared with nonexposed controls(33 men, 85 women). Male workers exposed to solvent mixtures had significantly lower plasma level of follicle-stimulating hormone(FSH) than nonexposed male controls. While female workers exposed to solvent mixtures had significantly lower plasma levels of growth hormone(GH), and thyroid-stimulating hormone(TSH) than nonexposed female controls. The results of significant decrease in plasma concentrations of pituitary hormones(FSH, GH, and TSH) in workers exposed solvent mixtures indicate that occupational exposure to solvent mixtures may induce neuroendocrine effects through an effect on hypothalamic pituitary axis.

상수 원수에 따른 용존 유기물의 특성 평가

허준무,박종안,손부순,장봉기,이종화 한국환경과학회 1999 한국환경과학회지 Vol.8 No.3

This study was carried out to evaluate the characteristics of dissolved organics based on their origins, which were divided into two categories. The first group consisted of river, lake and secondary sewage treatment effluent, which were chosen as representative of their origins. The second group were artificial samples which were made of AHA(Aldrich humic acids) and WHA(Wako humic acids). Physicochemical characteristics, biological degradability and THMFP(trihalomethane formation potential) of the samples were analysed based on the AMWD(apparent molecular weight distribution). Large portion of dissolved organic carbon(DOC) in the river and lake samples was comprised of LMW(low molecular weight), while that of AHA and WHA was HMW(high molecular weight). The DOC of the lake was evenly distributed in the all range of molecular weight. The river, lake and secondary treated effluent have lower ultraviolet(UV) absorbance at 254nm, and have a higher amount of humic acids. Higher absorbance of humic acids means that aliphatic bond and benzenoid type components that absorb UV light were contained in these kind of humic acids. It was expected that lake sample was the most biodegradable in the different samples investigated, and in order of secondary sewage treatment effluent, river, WHA and AHA based on the result of determination of specific ultraviolet absorbance(SUVA). Biodegradability showed similar result except for ABA, while dissolved organics in the range of LMW decreased during the biodegradability test, and on the contrary those of HMW increased. Production of the SMPs(soluble microbial products) was observed during humicification of dissolved organics and the SMPs were in the range of 0.7∼5.5% of DOC which was the lowest value. Sample had a higher portion of HMW showed a higher production of the SMPs. THM formation was high in the samples containing HMW and similar tendency was shown in the THMFP(trihalomethane formation potential), except for WHA.

안달루스 군소왕국 시대가 아랍문학에 끼친 영향

이종화 부산외국어대학교 지중해연구소 2004 지중해지역연구 Vol.6 No.1

`Al-Mansu￣r was a great statesman and a great soldier When he died in 1002 AD, The political situations in Andalus rapidly changed The unity of the Cordova khalifate passed away in 1031 AD admit violent internecine struggles in Andalus muslim society Berber and Slaves divided the kingdom between them, accordingly the muslim territory of Andalus was divided into kingdoms of the mulu￣k ´al-Tawa￣´if Of these Party Kings the most powerful were the `Abba￣dids of Sevillia Although it was an age of political decay, the material prosperity of Spain had as yet suffered little diminution, while in point of literature activity and cultural renaissance, the society of this time reached a level hitherto unequalled As regards poetry, the period of mulu￣k ´al-Tawa￣´if is the period of unsurpassed brilliancy, due mainly to the great freedom of behavior, which set aside many religious restrictions, especially those against wine drinking, those concerning the seclusion of women, the wearing of the veil, and love relations between the two sexes The first half of the 11 Century is marked by these great figures `Ibn Hazm, `Ibn Zaydu￣n, Wala￣dah. The second half of the century is dominated two figures `Al-Mu´ tamid the poet-king of Sevillia and His Wazir `Ibn ´Amma￣r

철강 제조업체 근로자에서 효소면역측정법을 통한 B형 간염 바이러스 표지자 조사

이종영,강승원,하명화,김중구,배삼덕,김두희,남복동 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.2

포항 지역 한 철강 제조업에서 1995년 1월부터 10월 사이에 실시한 정기 건강진단을 받은 남자 근로자 11,523명을 대상으로 HBsAg 및 Anti-HBs, HBeAg 및 Auti-HBe은 EIA법으로, SGOT 및 SGPT는 반응속도법(IU)으로 검사하였다. 그리고 이들 대상자에서 교육정보, 근무 형태, 음주 및 흡연습관과 간기능 이상 여부를 조사하여 다음과 같은 결과를 얻었다. 1. B형 간염 표지자의 분포를 보면 HBsAg(+)/Anti-HBs(-)는 8.1%, HBsAg(-)/Anti-HBs(+)는 60.5% 그리고 HBsAg(+)/Anti-HBs는 0.1%이었으며, HBsAg의 양성율은 8.2%, Anti-HBs 양성율은 60.6%이었다. 2. 연령에 따라 HBsAg(+)/Anti-HBs(-)와 HBsAg(-)-HBs(+)가 유의하게 증가한 반면(p<0.05), 교육정도와 근무형태에 있어서는 통계적으로 유의한 차이가 없었다. 3. 음주율은 HBsAg(+)/Anti-HBs(-)에서 HBsAg(-)/Anti-HBs(-)와 HBsAg(-)/Anti-HBs(+)의 84.6% 및 85.7%에 비해 낮지만 73.9%나 되었고, 흡연율은 HBsAg(-)/Anti-HBs(+), HBsAg(+)/Anti-HBs(-) 및 HBsAg(-)/Anti-HBs(-)에서 54.3%, 53.7% 및 53.0%의 순서로 나타났다. HBsAg(+)/Anti-HBs(-)에서 간기능 이상자가 40.1%이었으나 HBsAg(-)Anti-HBs(-)와 HBsAg(-)/Anti-HBs(+)에서도 각각 21.9% 및 18.4%를 차지하였다. 4. HBsAg(+)에서의 e항원에 대한 표지자의 분포를 보면 HBeAg(+)/Anti-HBe(-)는 31.3%, HBeAg(-)/AntiHBe(+)는 55.0% 그리고 HBeAg(+)/Anti-HBe(+)는 1.6%이었고 HBeAg(+)/Anti-HBe(-)는 연령에 따라 감소하고 HBeAg(-)/AntiHBs(-)와 HBeAG(-)/AntiHBe(+)는 증가하였다. HBsAg 양성자중 HBeAg의 총 양성율은 32.9%로 연령이 증가할수록 유의하게 감소하였다. 본 연구는 근로자의 B형 간염 표지자 조사에 있어 Anti-HBs의 검사에서는 EIA 방법으로 실시해야 하는 근거를 제시하는 것이며, 철강산업 근로자에서는 B형간염 이외의 간장질환에도 관심을 가져야 할 것으로 보이며 음주 문제에 대한 대책마련이 시급히 이루어 져야 할 것으로 생각된다. A study for seroprevalence of Hepatitis B virus markers (HBsAg, Anti-HBs, HBeAg and Anti-HBe) by EIA method and the Liver Function Test(SGOT and SGPT) using kinetic method was carried out from January to October 1995 among male workers of a major iron and steel manufacturing company located in Pohang. The results were as follows; 1. The distribution of hepatitis B virus markers showed that the proportions of HBsAg(+)/Anti-HBs(-), HBsAg(-)/Anti-HBs(+) and HBsAg(+)/Anti-HBs(+) were 8.1%, 60.5% and 0.1%, respectively. The positive rate of HBsAg in all subjects was 8.2%, whereas that of anti-HBs was 60.6%. 2. The proportions of HBsAg(+)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) increased significantly with age. While the distribution of hepatitis B virus markers exhibited no significant differences among the groups classified by educational background or work type. 3. The proportion of drinkers in HBsAg(+)/Anti-HBs(-)(73.9%) was significantly lower than those of HBsAg(-)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) (84.6% and 85.7%, respectively). The proportions of smoking for the three groups of HBsAg(-)/Anti-HBs(+), HBsAg(+)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(-) were 54.3%, 53.7% and 53.0%, respectively(p<0.01). The proportion of the subjects with abnormal liver function test in HBsAg(+)/Anti-HBs(-) was 40.1%, whereas those in HBsAg(-)/Anti-HBs(-) and HBsAg(-)/Anti-HBs(+) were 21.9% and 18.4%, respectively. 4. The distribution of e Ag markers among HBsAg(+) subjects showed that the proportions of HBeAg(+)/Anti-HBe(-), HBeAg(-)/Anti-HBe(+) and HBeAg(+)/Anti-HBe(+) were 31.3%, 55.0% and 1.6%, respectively. The proportion of HBeAg(+)/Anti-HBe(-) decreased significantly with age and those of HBeAg(-)/Anti-HBe(-) and HBeAg(-)/Anti-HBe(+) increased with age. The positive rate of HBeAg among HBsAg(+) subjects was 32.9%. In conclusion, as a screening method, EIA is recommended to test hepatitis B virus markers, especially when examining Anti-HBs more sensitively. For the workers of the iron and steel manufacturing company studied, liver diseases caused by other than hepatitis B should be given a special care including an education avoiding alcohol drinking.

B형 간염 바이러스 단백질에 있어서 HLA-A2에 의해 표현되는 Epitope 펩타이드 들의 분석

이희구,임종석,김승목,이기영,김희수,김승호,권태종,최인성,정태화,김길현 梨花女子大學校 藥學硏究所 1995 藥學硏究論文集 Vol.- No.5

The cytotoxic T lymphocyte (CTL) are an important component in host defense mechanism against viral infection. They can recongnize virus-derived peptides presented by the Class I MHC molecule at the cell surface of the infected cells. On searching for effective CTL epitopes of hepatitis B virus(HBV), we synthesized a distinct set of 9-10 mer peptide containing amino acid sequence of hepatitis B virus surface protein that are selected on the basis of a computer modeling and the previously described HLA-A2 specific motifs.Binding assay of the synthetic peptides to HLA-A2 molecules using human antigen processing defectant T2 cells showed that 3 out of 4 synthetic peptides enhanced the expression of HLA-A2 molecule on T2 cell surface.Two anchor positions, namely P2 and P9(or P10) appeared to play a decisive role for binding.Structural. characteristics of the peptides addressed by molecular dynamics simulation was analysed and compared.These peptides also partially triggered CTL isolated from human peripheral blood mononuclear cells of HBV positive patients, and the response was peptide-spcific.These results showed that negatively-charged amino acid residue at P2 hampered binding affinity of the peptides to HLA-A2 molecules, and that binding affinity of the peptides are not always reflected by their immunogenicity among natural T cell repertoire.

우리나라 화학물질 관련 사고 대비 및 대응체계 분석

이종화,장봉기,박종안,김은화,김춘득,여은영,남상훈 순천향대학교 기초과학연구소 2004 순천향자연과학연구 논문집 Vol.10 No.2

Recently, several large chemical accidents took place in many countries. Therefore, many countries strengthened the regulation related to management of chemical safety. In case of our country, about 500 chemical accidents took place for the past decay. The preparation and response system for chemical accidents is a most important element for management of chemical safety. In this study, we analyze the preparation and response system for chemical accidents described in several regulations of our country, and suggest a more appropriate management system for chemical safety. Accident report documents vary among the Toxic Chemical Control Law, the Fire Services Law and the Industrial Safety and Health Law. In case of the Fire Services Law, there is not a concrete description about the source chemical of accident. The response authority for chemical accident is dualistic. Minister of Government Administration and Home Affairs is responsible for emergent rescue, on the other hand, ministers of central administrative branches are in charge of reconstruction of chemical accident. Therefore these dualistic system make confuse in response to a large chemical accident.

충남 연기지역에서 발생한 쭈쭈가무시병 30예의 임상적 고찰

이종화,송민호,노흥규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.2

We experienced 30 Tsutsugamushi disease cases occuring in the Yongi area during late October t 1 early November 1992, diagnosed clinically and serologically by indirect immunofluorescent antibody test with the following results. 1. The disease was prevalent in adult female farmers and most of the patients had chances of exposure to grass fields or scrub. 2. Clinical findings were characterized by fever(100%), myalgia, abdominal pain, headache, rash(87%), eschar(50%), conjunctival injection, CVA tenderness and lymphadenopathy. 3. On laboratory findings, proteinuria(30%) and hematuria(17%) were observed and signs of interstitial pneumonia(7%) on chest X-ray film were noted. 4. All the patients showed mild clinical course and good response to antibiotics therapy with doxycycline or chloramphenicol.

골프장 이용객 특성과 인구통계학적 특성에 따른 골프장 서비스 중요도 차이

이우재,이종화 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.3

The purpose of this study was to identify service importance which golfer perceived on a variety of services. The 695 subjects (92.7%) were selected using quota sampling method from people who used a golf course in Kyunggi-do, The reliability of the questionnaire was Cronbach's α=.770 to ,872, and the validity was verified by the 5 sport marketing professors and 5 experts related a golf, The statistics were descriptive statistics, t-test, One-way ANOVA, and post-hoc comparison, The results were as follows. First, the importance degree of a golf course service was different to an utilization cost in gender, a resting place in married or not, a clubhouse, reservation, as utilization cost, a resting place, supplementary facilities, and customer convenience in a scholarship, an utilization cost and supplementary facilities in a type of occupation, reservation and an utilization cost in income, Second, the importance degree of a golf course service was different to customer convenience in member or nonmember, a clubhouse and a resting place in a number of membership card, reservation, an resting place, an utilization cost, and course administration in frequency of utilization, a clubhouse, reservation, an utilization cost and service before playing in reason of utilization.

인플레이션 期待下의 貨弊需要

李鐘和,孫東辰 湖西大學校工業技術硏究所 1985 工業技術硏究所論文集 Vol.4 No.-

Milton Friedman argued that physical goods should be regarded as a substitute for money and that higher expected rates of inflation should induce a portfolio shift from money to physical assets. As Friedman has derived his equation, the demand for real balances is a function of the expected rate of inflation in addition to nominal interest rates and real income. A number of empirical studies for different countries have shown that the anticipated rate of inflation has an independent direct effect on the demand for money, other than via interest rates. But in Korea, there are few studies about the role of expected rate of inflation in the demand for money. The purpose of this paper is to test the significance of the sorted-out independent effect of the expected rate of inflation on real balances and to suggest more appropriate monetary policy in such country suffering high inflation as Korea. In this paper a real partial adjustment mechanism of money demand is assumed. And the expected rate of inflation is estimated by the stochastic time series model(ARIMA model). According to the evidence of this paper, the expected rate of inflation exerts significant influences upon money holdings, while nominal rate fo interest has little explanatory power in money demand function. This confirms the fact that nominal rate of interest has been under the strict government control and has not reflected the opportunity cost of money holdings in Korea. These results suggest that monetary authority should control money supply more carefully in Korea, where everyone has inflationary expectations. In further studies particular emphasis should be given on the mechanism of price expectations formation associated with the appropriate specification of the money demand function and the correct forecast of the effect of monetary policy.