Infectious in vivo Transcripts from a Full-length Clone of Soybean mosaic virus Strain G5H

Seo, Jang-Kyun,Lee, Hyeok-Geun,Choi, Hong-Soo,Lee, Su-Heon,Kim, Kook-Hyung The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.1

An infectious full-length clone of Soybean mosaic virus (SMV) strain G5H was constructed under the control of the cauliflower mosaic virus 35S promoter. The cloned SMV G5H established infections upon simple rub-inoculation of soybean leaves with intact plasmid DNA. We demonstrated that this SMV G5H infectious DNA clone caused typical characteristic symptoms and virulence of SMV strain G5H in twelve tested soybean cultivars. Soybean cultivars Lee74, Somyungkong and Sowonkong developed systemic mosaic symptom while Kwanggyo, Taekwangkong, Hwangkeumkong and Geumjeongkong-l showed systemic necrosis. In contrast, Geumjeongkong-2, Jinpumkong-2, L29, V94-5152 and Ogden showed resistant response against SMV-G5H infection. We also determined full-length sequence of cloned SMV-G5H. The phyogenetic analyses reveal that SMV-G5H is most closely related to SMV-G5, and support that SMV-G5H might be derived from SMV-G5 by recombination rather than mutation.

요골동맥을 이용한 관동맥 조영술의 유용성

윤정한,이승환,이한요,김장영,김일회,최윤종,이형준,이명옥,김승년,황성오,홍인수,최경훈 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.10

광중합 시 수종의 심미적 수복재와 이장재의 사용에 따른 치수내 온도변화

장혜란,이형일,이광원,이세준 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Polymerization of light-activated restorations results in temperature increase caused by both the exothermic reaction process and the energy absorbed during irradiation. Within composite resin, temperature increases up to 20℃ or more during polymerization. But, insulation of hard tissue of tooth lowers this temperature increase in pulp. However, many clinicians are concerned about intrapulpal temperature injury. The purpose of this study was to evaluate temperature changes in the pulp according to various restorative materials and bases during curing procedure. Caries and restoration-free mandibular molars extracted within three months were prepared Class I cavity of 3×6mm with high speed handpiece fissure bur. 1mm depth of dentin was evaluated with micrometer in mesial and distal pulp horns. Pulp chambers were filled with 37.0 ±0.1℃ water to CEJ. Chromium-alumina thermocouple was placed in pulp horn below restorative materials for evaluating of temperature changes. This thermocouple was connected to temperature-recording device(Multifunction analyzer MX, 6.000, JAPAN). Temperature changes was evaluated from initial 37.0℃ after temperature changes to 37.0℃. Tip of curing unit was placed in the center of prepared cavity separated 1mm from restorative materials. Curing time was 40s. The restorative materials were used with Z 100, Fuji II LC, Compoglass flow and bases were used with Vitrebond. Dycal. Resrorative materials were placed in 2mm. The depth of bases were formed in 1mm and in this upper portion, resin of 2mm depth was placed. This procedure was performed 10 times. The results were as follows : 1.All the groups showed that the temperature in pulp increased as curing time inceased 2.The temperature increase of glass ionomer was significantly higher than that of Resin and Compomer during curing procedure (P<0.05). 3.The temperature increase In glass ionomer base was significantly higher than that of Calcium hydroxide base during Resin curing procedure (P<0.05).

R-12 냉매용 냉동장치의 성능에 미치는 R-12와 R-134a 냉매의 효과

이홍기,장동호,정용진,강형석 강원대학교 산업기술연구소 2000 産業技術硏究 Vol.20 No.B

High pressure, pressure ratio, refrigerating effect, heat transfer from the condenser and the power of the compressor etc. of a self-made refrigeration equipment for R-12 are investigated when R-12 and R-134a are used as the coolants. The comparison between the performance for R-12 and that for R-134a is made. As a result, R-134a is better than R-12 in the view of high pressure, refrigerating effect and the coefficient of performance and vice versa in the view of pressure ratio, exit gas temperature from the compressor and heat transfer from the condenser.

개에 발생한 만성 승모판폐쇄부전증에 대한 Pimobendan 치료 1례

장재임,전형규,최호정,이영원,송근호 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2006 動物醫科學硏究誌 Vol.14 No.-

A 11-year-old intact male maltese dog with respiratory distress, cyanosis and coughing was referred to the Veterinary Medical Teaching Hospital of Chungnam National University. Loud holosystolic murmur with a palpable precordial thrill was heard at the left apex region by auscultation. Left sided cardiac enlargement and mitral regurgitated flow were detected by thoracic radiography and echocardiography, respectively. Clinical signs were improved by treatment of diuretics, ACE inhibitor and pimobendan.

정보시스템 감리의 모형설계와 개선에 관한 연구

이장형,박희석 大邱大學校 社會科學硏究所 2000 社會科學硏究 Vol.8 No.1

정보화 시대를 살아가면서 더 많은 조직들이 업무에 있어서 정보의 중요성을 인식하고, 대부분의 조직들은 정보시스템을 주된 기능으로 대체하는데 기초를 둔 컴퓨터 환경으로 변화시킬 계획들을 가지고 있다. 하지만 많은 조직들은 정보시스템의 효율성과 신뢰성을 조사할 필요가 있고 심지어 불평까지 하는 경우도 있다. 오늘날 정보시스템 감리에 대한 수요는 정보시스템의 성공적인 개발을 지원하기 위해 증대하고 있다. 감리의 목표는 정보시스템의 부작용을 막고, 예산의 효율적인 사용을 확신하며 조직의 내부 감리 능력과 인식을 제공하는 것이다. 이런 목표를 성취하고 감리 품질을 높이기 위하여 정보시스템 감리를 제도화하고 감리모형을 개발할 필요가 있다. 이 연구에서는 우선 감리, 모형, 사례등을 기술한다. 그리고 감리를 수행하기 위한 모형과 방법을 소개한다. 이 모형들은 시스템 개발자와 감리인에게 사용되어질 것이다. 감리의 증대되는 수요에 따라 새로운 모형의 개발은 필요하고 현장에서 응용도구와 다양한 기술 표준 및 모형이 고려되었다. 따라서 정보시스템 감리 모형에 관한 연구를 수행하였다. 정보시스템 감리의 모형 개발을 위한 하위 메뉴로는 정보시스템 계획을 위한 감리모형, 정보 시스템 관리를 위한 감리모형, 정보시스템 유지를 위한 감리모형, 정보시스템에서 품질관리를 위한 감리모형, 보안 및 통제를 위한 감리모형이다. 모형은 3가지 모형인데 모형1은 동등비중의 감리모형, 모형2는 점검표 항목수에 따른 감리모형이고 모형3은 이 둘의 혼합 감리모형이다. 개선되는 모형으로는 모형3을 제안한다.

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

개인방사선피폭선량평가

이형섭,김장렬,윤석철,이상윤 대한방사선 방어학회 1994 방사선방어학회지 Vol.19 No.2

최근, 개인방사선피폭선량평가에 관한 기술기준이 과학기술처에 의해 고시됨에 따라 국내에서도 개인선량계를 이용한 체외피폭선량평가결과의 신뢰성 문제가 크게 대두되고 있다. 한국원자력연구소에서는 이러한 구내의 상황을 인식하고 자체적인 신뢰성 확보를 위해 미국의 Oak Ridge국립연구소의 주관하에 개인피폭선량의 평가에 관한 국제상호비교검증시험(Personnel Dosimetry Intercomparison Study ; PDIS) 을 수행하였다. 비교 검증시험에는 한국원자력연구소에서 사용하고 있는 Teledyne PB-3 열형광선량계가 사용되였으며 선량게산 알고리즘은 Teledyne PB-3 version1.5-1989를 사용하였다. 본 연구에서는 지금까지 실시된 PDIS의 결과를 요약하고 현재 PB-3 시스템의 개인선량평가성능에 대해 고찰하였으며, 선량평가절차에 대한 문제점 도출을 통하여 직업적 방사선피폭선량 평가능력의 향상을 위한 방안을 제시하였다. Recently, the Ministry of Science and Technology issued a Ministerial Ordinance (N0 1992-15) about the technical criteria on personnel radiation dosimetry. In today's climate, it is important to demonstrate and document that the processor's systems and services to others meet national standards of quality. The purpose of this study is to verify the performance of the Teledyne PB-3 personnel dosimetry system that is generally used in Korea Atomic Energy Research Institute (KAERI) by intercomparison with Oak Ridge National Laboratory. The KAERI has been participated in this personnel dosimetry intercomparison study(PDIS) program since 1991 and it could be possible to test and calibrate personnel monitoring system. This report presents a summary and analysis of by about 50 does equivalent measurements reported for PDIS-16 through 18 (1991-1993) with emphasis on neutron does equivalent sensitivity, accuracy and methods to improve personnel dosimetry performance are also discussed.

내측 측두엽 간질에서 간질발작 중 언어증상

이장준,이승환,이형,김지언,임정근,손은익,이상도,박영춘 계명대학교 의과학연구소 2000 계명의대학술지 Vol.19 No.1

Background and Objectives : Several speech manifestation are observed in temporal lobe epilepsy(TLE). We studied the frequency of ictal speech manifestations and their localizing and lateralizing value in TLE. Methods : We reviewed 379 seizures recorded on videotape in 135 patients who underwent temporal lobectomy for intractable TLE. We divided patients into group A(n=75) who became seizures free after surgery, and group B(n=60) who were only slightly benefited or unchanged after surgery. Speech manifestations were classified as normal speech, vocalization, or abnormal speech. Abnormal speech was subdivided into speech arrest, dysarthria, dysphasia, or nonidentifiable speech. Results : Speech manifestations occurred in 70(18.5%) seizures of 28 patients. Forty eight seizures of 20 patients were observed in group A, and 22 seizures of 8 patients were observed in group B. In group A, 25 seizures of 11 patients were originating from dominant hemisphere, 23 seizures of 9 patients from nondominant hemisphere. In group B, 13 seizures of 5 patients were originating from dominant hemisphere, and 9 seizures in Lt. side and 6 seizures in Rt. side, respectively) of group A(P>0.05), and eleven seizures of 4 patients(8 seizures from Lt. side and 3 seizure from Rt. side) in group B(P>0.05). Normal speech was observed only seizures from Rt. side in both groups(3 seizures in 1 patient, each group). Dysphasia occurred only in seizures originating from Lt. side. Nonidentifiable speech occurred in 22 seizures of 9 patients(11 seizures from each side) in group A(P<0.01), and 3 seizures of 1 patient occurred only from Lt. side in group B. Conclusion : Ictal speech manifestations were not uncommon(18.5%). All kinds of ictal speech manifestations except normal speech have no reliable lateralizing and localizing value in TLE. Normal speech could be a good indicator of seizure originating from nondominant hemisphere.

PGE_2와 DBcAMP가 골조직 세포의 생리적 활성에 미치는 영향

이준기,채한정,강장숙,김형룡 원광대학교 생체재료·매식연구소 1998 원광생체재료·매식 Vol.7 No.2

One of the primary functions for which bones have evolved is to act as structural support. To achieve this goal, bones are remodeling throughout life so that their structure remains optimal for the prevailing mechanical environment. Bone remodeling consists of an initial phase of osteoclastic bone resorption followed by a bone formation period. Prostaglandins are potent regulators of bone formation and bone resorption that can have both stimulatory and inhibitory effects. Elevation of intracellular cAMP is an important intracellular signaling mechanism involved in the regulation of the expression of many proteins. In this study, the author examined whether PGE_2 or DBcAMP affected osteoblastic activation or osteoclastic differentiation in mouse bone marrow cells and osteosarcoma ROS 17/2.8 cells. The effect of PGE_2 and DBcAMP on the cell proliferation was measured by the incorporation of [^3H]-thymidine into DNA. From the result, PGE_2( 0.5-1 ㎍/ml ) and DBcAMP(0.1-0.5 mM) inhibited the [^3H]-thymidine incorporation into DNA. The effect of PGE_2 and DBcAMP on the induction of alkaline phosphatase(ALP) was investigated in ROS 17/2.8 cells cultured in medium containing 0.4% fetal bovine serum. PGE_2 and DBcAMP stimulated ALP activity in the cells. PGE_2 also increased the intracellular cAMPs contents with a maximal effect at 0.5 ㎍/ml. ROS 17/2.8 cells released nitric oxide upon stimulation of PGE2 or DBcAMP with interferon-γ. PGE_2 and DBcAMP increase the phosphorylation level of CREB(cAMP response element binding protein) without any change on the amount of CREB protein. Also, PGE_2 (10^-6 M) and DBcAMP (10^-4 M) significantly increase the generation of osteoclast-like cell in mouse bone marrow cell culture system. In conclusion, the results of this study suggested that PGE_2 and cAMP should appear to be an important regulatory molecule in the processes of bone formation and resorption.