A selective cyclin-dependent kinase 4, 6 dual inhibitor, Ribociclib (LEE011) inhibits cell proliferation and induces apoptosis in aggressive thyroid cancer

Lee, Hyun Joo,Lee, Woo Kyung,Kang, Chan Woo,Ku, Cheol Ryong,Cho, Yoon Hee,Lee, Eun Jig Elsevier 2018 Cancer letters Vol.417 No.-

<P><B>Abstract</B></P> <P>The RB-E2F1 pathway is an important mechanism of cell-cycle control, and deregulation of this pathway is one of the key factors contributing to tumorigenesis. Cyclin-dependent kinases (CDKs) and Cyclin D have been known to increase in aggressive thyroid cancer. However, there has been no study to investigate effects of a selective CDK 4/6 inhibitor, Ribociclib (LEE011), in thyroid cancer. Performing Western blotting, we found that RB phosphorylation and the expression of Cyclin D are significantly higher in papillary thyroid cancer (PTC) cell lines as well as anaplastic thyroid cancer (ATC) cell lines, compared with normal thyroid cell line and follicular thyroid cancer cell line. LEE011 dose-dependently inhibited RB phosphorylation and also decreased the expressions of its target genes such as <I>FOXM1, Cyclin A1,</I> and <I>Myc</I> in ATC. Furthermore, LEE011 induced cell cycle arrest in G0-G1 phase and cell apoptosis, and inhibited cell proliferation in ATC. Consistently, oral administration of LEE011 to ATC xenograft models strongly inhibited tumor growth with decreased expressions of pRB, pAKT and Ki-67, and also significantly increased tumor cell apoptosis. Taken together, our data support the rationale for clinical development of the CDK4/6 inhibitor as a therapy for patients with aggressive thyroid cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> pRB and Cyclin D were expressed high in aggressive thyroid cancer. </LI> <LI> LEE011 suppressed pRB and also decreased the expressions of its target genes in ATC. </LI> <LI> LEE011 induced cell cycle G1 arrest and apoptosis, and inhibited cell proliferation. </LI> <LI> LEE011 inhibited in vivo tumor growth with decreased expressions of pRB and Ki-67. </LI> <LI> We could explain the anticancer effects with the RB-E2F pathway. </LI> </UL> </P>

Reported speech in conversation : A discourse analytic approach

Lee, Eun-Joo 이화여자대학교 사범대학 교과교육연구소 2003 East west education Vol.20 No.-

The study examines two different types of reported speech: explicit reported speech and nonexplicit speech in an attempt to analyze how reported speech is produced and interpreted in daily conversations. The results of the analysis clearly support the claim that what is reported in the reported speech is not only the speech itself but also the mental state description. The analysis also shows that factors such as linguistic and paralinguistic features as well as circumstances in which conversation takes place all play an important role in correct interpretation of the reported speech in natural conversations.

李應台(1556~1586) 묘 출토 상의류의 분류와 구성법

이은주(Lee Eun-Joo) 한복문화학회 2002 韓服文化 Vol.5 No.3

This study proposes a classification standard of the upper garment based on the excavated costume from the grave of Eung- T ae Lee(1556-1586) found at JeongSang-dong, Andong, KyungBuk province, Korea on APril 1998. This study also classifies the remaining clothes by the proposed standard and shows their characteristics and constructive methods.<br/> The first classification standard of the upper garment is based on the assumption that the clothes cover the upper<br/> body are the male upperwears.<br/> The second classification standard is based on the whole scale such as back length, width, and sleeve length and the third one is based on the other components including the shape of collar, length of side-open, armpit gusset. materials. arrangement of color.<br/> The upperwears from the grave of Yeung- Tae Lee consist of 7 pieces for male, 3 pieces for female. and 1 piece for children. Jukori. Hansam. and Jucksam are classified into male upperwear category, Jangjugori without side-open and Danjugori without side-open are classified into female upperwear category.<br/>

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

I'll be like Princess Pari, and I’ll rescue you: Beccah’s Development into a Postcolonial Female Writing Subject in Comfort Woman

( Lee¸ Eun-joo ) 동국대학교 영어권문화연구소 2015 영어권문화연구 Vol.8 No.2

This essay discusses Beccah’s development into a postcolonial female writing subject represented in Nora Okja Keller’s Comfort Woman, to which critics have hitherto less paid their critical attention. Based on major theories concerning the Bildungsroman and the postcolonial female Bildungsroman, the essay begins its discussion by defining the novel as a postcolonial female Bildungsroman. The essay then investigates why her mother’s shamanism, which is the symbol of her postcolonial female legacy, functions as Bildung for Beccah’s development. Building on this investigation, the essay discusses not only what Beccah learns from Bildung from her mother’s postcolonial female shamanism but also how her development is differentiated from her mother’s shamanism. The later part of this essay dedicates itself to analyzing Beccah’s narrational strategies implied from formal and thematic characteristics of her narrative. It can be implied from this analysis that her authorship of the novel is her own way of realizing justice for her mother and other Korean comfort women. The ultimate purpose of this essay is to prove that Beccah’s writing Comfort Woman is itself not only the evidence of her development but also one example of a mature and responsible attitude as a writing subject.

Repellent efficacy of essential oils and plant extracts against Tribolium castaneum and Plodia interpunctella

Lee Hwa‐Eun,Hong Su Jung,Hasan Najmul,Baek Eun Joo,Kim Jun Tae,Kim Young‐Duk,Park Mi‐Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.9

This study was conducted to investigate the repellent efficacy of essential oils (Origanum vulgare, Pimpinella anisum, and Tanacetum cinerariifolium) and four plant extracts (Agastache rugosa, Capsicum annuum, Citrus reticulata, and Ginkgo biloba) against Tribolium castaneum (adults and larvae) and Plodia interpunctella (larvae). Gas chromatography/mass spectrometry analysis revealed the presence of carvacrol, anethole, and jasmolin I as the predominant constituent in O. vulgare, P. anisum, and T. cinerariifolium, respectively. Furthermore, ethyl hexopyranoside, 9,12‐octadecadienoic acid, cyclopentanol, and 2‐cresol were identified in A. rugosa, C. annuum, C. reticulata, and G. biloba, respectively. The repellent efficacy of each essential oil, plant extract, and the combination of oils was evaluated using a specially designed cylinder trap for 120 h. Among the three oils, O. vulgare and T. cinerariifolium had greatest repellent efficacy against P. interpunctella larvae. T. cinerariifolium exhibited effective repellence against the adults and larvae of T. castaneum. Therefore, O. vulgare (O) and T. cinerariifolium (T) were selected for further investigation of combined effects. Two essential oils were mixed in three different ratios of OT1 (1:3), OT2 (1:1), and OT3 (3:1). The repellent efficacies of OT1 and OT2 against the adults of T. castaneum were significantly greater than that of OT3. OT1 was effective against the larvae of T. castaneum, whereas OT2 was effective against the larvae of P. interpunctella. OT1 enhanced the repellent efficacy by approximately five times against larvae of T. castaneum, compared with that of T. cinerariifolium. Overall, OT1 was selected as the best repellent substance against all the tested insects.

조선시대 품대의 구조와 세부 명칭에 관한 연구

이은주(Eun Joo Lee) 한국복식학회 2011 服飾 Vol.61 No.10

The ranking belts for Joseon dynasty officials that are based on references and relics are studied in this paper in terms of architecture, detailed names, and structural changes according to different time. Officials` uniforms consist of hats, clothes, belts, and shoes. Among these, the belt is an important sign that represents the wearer`s ranking. The ranking belts of the Joseon dynasty which were brought from Ming at the late stage of the Koryo dynasty became classified as the following four classes : Seo-dai(a rhinoceros` horn, 犀帶), Gum-dai(gold, 金帶), Eun-dai(silver, 銀帶), and Heug-gag-dai(black horn, 黑角帶). A ranking belt consists of a basic belt body(程) and a plaque that represents the wearer`s rank. A plaque consists of 20 plates: three front-center plates that represent the Sam-tai(三台) constellation, six front-side plates that represent the Namduyug constellation(南斗六星), seven back plates that represent the Big Dipper(北斗七星), left side Bo(輔), right side Pil(弼), and a couple of Tamie at both ends. The architecture of the belt body; the basic frame for ranking belts, shows some differences between the former and the latter periods of the Joseon dynasty. In the former period, the belt had a pair of a buckle so that the wearers were able to adjust the belt size. But later, the belt didn`t have the buckles to adjust the belt size and consequently it only performed a locking or unlocking function. Therefore, the belts in the latter period were longer than normal and one size fit all. In addition to the functional change of buckles, the shapes of the ranking belts show changes from the round shape to the square shape as time goes on.

1800년 정조(正祖) 국장(國葬) 재현을 위한 의장군(儀仗軍) 복식 연구

김은진(Kim Eun-Jin),이은주(Lee Eun-Joo) 한복문화학회 2016 韓服文化 Vol.19 No.1

The purpose of this study is to examine Uijang-gun(儀仗軍) costume that directly designates guards for the king to enable an appropriate reenactment of a national funeral for King Jeongjo(正祖) in 1800. Through field investigations of such representative royal ritual events as Royal Cultural Festival and “King Danjong’s(端宗) Cultural Festival”, it was found that the historical research was mostly reliable, but that there were a few differences between the reenactments and the historical event. These errors were caused by the consultation of Uigwe and ceremonial books and a failure to refer to details that reflect circumstances of the time before relying too heavily on the contents of Banchado(班次圖) and ceremonial books. As the national funeral given to King Jeongjo was the first funeral for a king after the massive overhaul of ancestral rites in the late Joseon dynasty, it carries significance. In particular, since “Jeongjo-Gukjang-Dogam-Uigwe (正祖國葬都監儀軌)” describes matters related to ceremonial equipages in great detail compared to other Uigwe(儀軌), it is likely to be useful in studying ceremonial equipages and ceremonial regiment costume in 1800. The details of Banchado can be checked through official documents exchanged among government offices such as Gyesa(啓辭), Imun(移文), Pummok(稟目) and Gamgyeol(甘結). Ceremonial regiment costume can be divided into three types according to its classification. Uijanggi-gun(儀仗旗軍) wore Cheong-ui(靑衣) and Pi-rip(皮笠), and San-seon-si-wi(繖扇侍衛) and Eun-ma-gwe-gun(銀馬机君軍) wore Cheong-ui and Ja-geon(紫巾) and Uijang-mul(儀仗物) and Gimul-gun(器物軍) wore Hong-ui(紅衣) and Ja-geon. Cheong-ui and Hong-ui took the form of So-chang-ui(小氅衣) made with cotton, which is a long gown with a side slit and narrow sleeves. Pi-rip is a red hat in the form of Cho-rip(草笠), and Ja-geon is a rectangular hat made with cotton. In addition to these, Hong-mok-dae(紅木帶) was worn, and Cheong-saek-Je-bi-haeng-jeon(靑色耳行纏) is applied to complete the ceremonial regiment costume.

식품 기준·규격의 합리적 관리 ·운영에 관한 연구(Ⅰ) : 두부,식용유지, 전분의 분말상 원료에 관하여 Powdered marerials of Tofu, Edible oil, Starch

김희연,홍진환,박혜경,한상배,박종석,이은주,이정성,송경희,최은희,최영준,소경아,성영제,이주엽 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

본 연구는 분말상 원료(전분, 대두분)의 저장유통시 품질변화를 건전성과 안전성 측면에서 검토하여 식품의 기준 ·규격 설정f.」 합지적으로 반영하고자 하였다. 전분과 패두분의 이물을 검사하고, 2종 대두분(중국산, 미국산)을 ?0일간 IS'c와 30t에 저장하면서 성상, 수분, 산가, 과산화물가, 지방산 조성,아플라톡신 BB의 생성여부를 분석하였다. 중국산 대두분 30'』 저장군에서 50일 이후 곰팡이가 발생한 것 이외에 모든 시료에서 양호한 성상을 나타내었고, 전분과 대두닥 모든 시료에서 이물은 검출되지 않았다. 산가는 중국산과 미국산 시료간에 편차를 보여 증국산 30"C, 50일 저장시료에서 약 10배끙도 증가하였고, 과산화물가는 저장 90일후 15'E 저장군은 약 2.3배, 30'c 저장군은 3배정도 증가하였다. 대두분의 주요 지방산은 linoleic acidf18. 2), oleic acid(18 : 1), palmitic acid(16 : 0)이었으며, 3개월 저장에 따른 지방산 함량의 변화는 미미하였다. 아플라톡신 B₁은 모든 시료에서 검출되지 않았다. The objective of this study was to investigate auality changes of type raw materials(starch, soy flour) during various storage conditions. Starchflour(source of China, U.S.A) were slfred under two temperature(15'C, 30'c) forSensory evaluation, (o.reign material test, moisture content, acid value, peroxide ualue,composition, aflatorin Bi were analyzed. China soy flour(stor·ed 30'f) was contaminated by fungj on 50 days. Except for soy flour contardnated by fungi, sensory characteristics were not changed and foreign mater;als were notdetected. Acid value of China soy flour(stored at 30'C, 90 days) was about 10 times higherlevel before storage and acid value was more influenced by source(China, USA). Peroxide valuewas in proportion with the storage period slightly. The major fatty acid of soy flour waslinoleic acid(18 . 2), an(3 the change of fatty acid composition was not observed. Aflatoxlrl Blwas not detected.

Predicting postoperative total calcium requirements after parathyroidectomy in secondary hyperparathyroidism

( Byung Heon Kang ),( Soon Young Hwang ),( Jeong Yeop Kim ),( Yu Ah Hong ),( Mi Yeon Jung ),( Eun Ah Lee ),( Ji Eun Lee3 ),( Jae Bok Lee ),( Gang Jee Ko ),( Heui Jung Pyo ),( Young Joo Kwon ) 대한내과학회 2015 The Korean Journal of Internal Medicine Vol.30 No.6

Background/Aims: To prevent hypocalcemia after parathyroidectomy (PTX), parenteral calcium is required in addition to oral calcitriol and calcium. After switching to oral calcium, patients can be discharged from the hospital. The aim of this study was to analyze the clinical characteristics and outcomes of PTX performed at a single Korean center and to investigate the associated laboratory factors used to analyze the total amount of postoperative calcium required. Methods: We enrolled 91 hemodialysis patients undergoing PTX from November 2003 to December 2011. We collected clinical and laboratory data preoperatively, 12 and 48 hours postoperatively, at discharge, and 3 and 6 months postoperatively. Results: In total, 59 patients underwent PTX with autotransplantation (AT), 6 underwent total PTX without AT, 11 underwent subtotal PTX, and 15 underwent limited PTX. Total PTX without AT showed the lowest recurrence rate. At all postoperative time points, the mean levels of serum calcium, phosphorus, and intact parathyroid hormone (iPTH) decreased significantly, compared with preoperative levels; however, alkaline phosphatase (ALP) increased significantly from 48 hours postoperatively to discharge (p < 0.001). On multiple linear regression analysis, the total amount of injected calcium during hospitalization showed a significant correlation with preoperative ALP (p < 0.001), preoperative iPTH (p = 0.037), and Δphosphorus at 48 hours (p < 0.001). We developed an equation for estimating the total calcium requirement after PTX. Conclusions: Preoperative ALP, preoperative iPTH, and Δphosphorus at 48 hours may be significant factors in estimating the postoperative calcium requirement. The formula for postoperative calcium requirement after PTX may help to predict the duration of postoperative hospitalization.