Time-Related Changes in Detrusor Overactivity in Awake Rats with Spinal Cord Injury Observed by Simultaneous Registrations of Intravesical and Intraabdominal Pressures

Jin, Long-Hu,Shin, Hwa-Yoan,Yoon, Seung-Hwan,Seong, Do-Hwan,Park, Chang-Shin,Lee, Tack,Yoon, Sang-Min Korean Continence Society 2011 International Neurourology Journal Vol.15 No.1

<P><B>Purpose</B></P><P>To compare the physical characteristics of detrusor overactivity (DO) induced by intravesical infusion of saline in awake, sham rats and rats with chronic spinal cord injury (SCI), by simultaneous registrations of intravesical and intraabdominal pressures.</P><P><B>Methods</B></P><P>Male Sprague-Dawley rats, normal or with a spinal vascular clip at the level of Th9, were investigated cystometrically 1 and 4 weeks after SCI. Intravesical pressure (IVP) and intraabdominal pressure (IAP) were recorded simultaneously to evaluate true DO. During the filling phase, the event of IVP rises, defined as increments that exceeded 2 cmH<SUB>2</SUB>O from baseline, were determined as DO according to the absence of simultaneous changes in IAP.</P><P><B>Results</B></P><P>All SCI rats exhibited DO during the filling phase, which was not shown in sham rats. The frequency and pressure of DO had a tendency to decrease with time. The DO frequency of SCI rats after 4 weeks (0.9±0.2 min<SUP>-1</SUP>) was decreased compared with that after 1 week (2.1±0.4 min<SUP>-1</SUP>; P<0.05). The DO pressure of SCI rats after 4 weeks (8.4±1.9 cmH<SUB>2</SUB>O) was decreased compared with that after 1 week (11.6±2.9 cmH<SUB>2</SUB>O; P>0.05).</P><P><B>Conclusions</B></P><P>Cystometric studies in awake male SCI rats showed some significant changes in bladder function after SCI. All SCI rats exhibited DO during the filling phase, and showed different physical characteristics of DO over the course of time. The neurological basis of these time-related changes remains poorly understood, but may provide important prognostic information about long-term urological management in SCI patients.</P>

Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

Benzo(a)pyrene에 의해 유도된 간기능 장해에 미치는 당귀의 효과

윤수홍,조수열,이윤경,하두현 한국환경독성학회 1992 환경독성보건학회지 Vol.7 No.1

This study was performed to investigate the effect of Angelicae gigantis Radix extract on the hepatic detoxifying enzyme activities and lipids. Male sprague-dawley rats were administrated the extracts with benzo(a)pyrene, a hepatotoxic agent, inducing liver damages. Results obtained from this study were as follows: 1. The markedly increased enzyme activities (AST, ALT, LDH, ALP, γ-GTP, GSH -Px) in B(a)P induced groups tended to be decreased by the treatment of the Angelicae gigantis Radix extract. 2. Liver GSH content and lipid peroxide activity were decreased by the administration of the extracts. 3. It tended that the curative effects were better than the protective effects of the extracts.

후두 및 하인두 편평세포암종에서 E-cadherin의 면역조직화학적 발현

도남용,나한조,이도용,허준,최지윤,이홍영,임성철 조선대학교 2000 The Medical Journal of Chosun University Vol.25 No.2

Background and Objectives : The cell-cell adhesion molecule E-cadherin is necessary for the maintenance of the epithelial cellular structure. We were designed to confirm the significance of E-cadherin as a marker for differentiation and invasiveness of squamous cell carcinoma of the larynx and hypopharynx. Materials and Methods : Our study was investigated for the immunohistochemical expression of the epithelium-specific cell adhesion molecule E-cadherin in formalin fixed paraffin embedded tissue specimen of 32 cases of squamous cell carcinoma of larynx and hypopharynx. Results : The positive expression of E-cadherin was 56.3%(18 cases) in squamous cell carcinoma of the larynx and hypopharynx. Decreased E-cadherin expression was a stastistically significant correlation with a increased grade of lymph node stage and clinical stage. Reduced expression was seen in the large tumor size and poorly differentiated tumors, but these result was not statistically significant. Conclusion : The expression of E-cadherin may be related with progression of squamous cell carcinoma of the larynx and hypopharynx. But these correlation were not sufficient for the prognostic indicators in squamous cell carcinomas of the larynx and hypopharynx.

인간 재조합 인터루긴-32 면역조절작용에 대한 유세포 분석

이광수,김영관,채정일,심정현,김은미,강형식,김수현,윤도영,명평근 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

Xenotransplantation of porcine organs has the potential to overcome the severe shortage of human tissues and organ available for human transplantation. however, it remains various hurdles for clinical xenotransplantation. In pig and mouse xenotransplantation, porcine xenograft evoke a strong cellular rejection response in immunocompetent host and grafts are destroyed within a week. This cellular immune response could involved both T cells and NK cells. A number of groups have shown that human NK cells can recognize and damage porcine endothelial cells. In addition, human T cells can respond to porcine endothelial cells through both direct and indirect mechanisms. Cellular rejection of porcine tissues requires T cells, particularly CD4^(+) cells. A new cytokine recombinant human interleukin-32α,β(IL-32α,β) has a role innate and acquired immune system. In order to investigate the role of recombinant mouse IL-18 and recombinant human IL-32α,β in xenograft rejection, we transplanted the PK(15) cells to C57BL/6 mice with or without intraperitoneal injection of recombinant mouse IL-18 or recombinant human IL-32 α,β. It was analyzed the population of NK cell, T cell and B cell in the C57BL/6 mice transplanted with PK(15) cells and recombinant mouse IL-18 or recombinant human IL-32α,β by flow cytometry analysis. As a result, lymph node and thymus of PK15/IL18, PK15/IL32α and PK15/IL32β injected group were increased to T cell activation population than normal injected groups. CD8^(+) T cells were decreased in lymph node of PK15/IL18, PK15/IL32α and PK15/IL32β injected groups. CD4^(+) T cells were increased in lymph node cell of PK15/IL32α and PK15/IL32β injected group and also, B cell population were increased in lymph node cell and spleen of PK15/IL18, PK15/IL32α and PK15/IL32β injected group. Therefore, we suggest that recombinant mouse IL-18 and recombinant human IL-32α,β suppress xenograft rejection in cellular xenotransplantation.

Z_nF_2:M_n薄膜의 電場發光에 관한 硏究

李相潤,金道聖,朴相鉉 경북대학교 교육대학원 1980 논문집 Vol.12 No.-

Thin films of ZnF_2:Mn sandwiched by SiO semi-insulating layers were prepared using the thermal vacuum evaporator. These films showed both AC and DC electroluminescence (EL). The brightness B was measured as a function of applied voltage V. Then the characteristic hysteresis curve was observed in B-V relation. We could see the photoluminescence (PL) in the crystalline powder of ZnF_2: Mn, and the peak of emitting spectrum in EL was the same as in PL.

마이크로 프로세서에 의한 自己制御형 永久磁石 同期 電動機의 驅動 및 運轉 特性의 改善

윤병도,김윤호,김일환,이용석 중앙대학교 기술과학연구소 1989 기술과학연구소 논문집 Vol.19 No.-

전력 전자 공학의 발달에 의해 영구자석 동기전동기의 운전 분야가 대단히 진보되고 있음이 최근 논문을 통해서 보고 되고 있다. 영구자석 동기전동기는 크기와 중량의 감소로 인한 장점, 회전자의 동손이 없으므로 효율이 높다는 장점이 있어 각종 분야의 응용에 유리하다. 본 연구는 마이크로프로세서와 전압원 인버터를 사용한 자기제어형 영구자석 동기전동기의 구동장치를 제안한다. 영구자석 동기전동기의 운전은 고정자 전류와 역기전력을 동기화 시킴으로서 가능하게 한다. 고 분해능의 인크리멘탈 엔코더를 사용하여 영구자석 동기전동기에 적합한 자기제어형 PWM. 인버터 설계를 가능하게 하였다. 저속 영역에서 토오크 리플을 감소 시키기 위해 펄스 수를 제어하였으며 운전중 최적 부하각의 제어는 토오크-속도 특성을 개선시킴을 검토 하였다. Recent research has indicated that the permanent magnet synchronous motor(PMSM) drives are considerably improved by the development of power electronics. The PMSMs are favoured in many applications as there are advantages in size and weight reduction, and in efficiency due to elimination of the field copper losses. This paper presents a Self-Controlled PMSM, fed by voltage suource inverter based on the microprocessor. The proper operation of the PMSM can be ensured only if the stator current is always synchronized with is Back-EMF. The use of incremantal encoder with high resolution makes it possible to design a Self-Controlled PWM inverter for a PMSM. The pulse ratios are controlled to reduce the torque ripple in the low-speed drive region. In addition, torque-speed characteristics are improved by the control of optimal load angle.

완전 포상기태 이후 발생한 지속성 융모질환의 치료경험 2례.

이진희,류은석,김윤숙,김종수,최승도,선우재근,배동한 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Persistent tumor develops in approximately 20% of patients with a complete mole, and chemotherapy is required to achieve remission. Following evacuation of hydatidiform mole, careful hCG monitoring is mandatory since it is the most reliable and sensitive method for the early detection of gestational trophoblastic disease. In carefully selected patients in whom the risk of developing gestational trophoblastic disease is significant or when the availability of hCG testing is suboptimal, chemoprophylaxis has been shown to decrease the risk of gestational trophoblastic tumor. We report here two cases of patient, 16-year-old and 45-year-old women who experienced unusual course after the evacuation of a complete mole and markedly elevated serum β-hCG levels. The patient developed persistent gestational trophoblastic disease and were successfully treated with EMA-CO and hysterectomy.

협기성 생물막 반응기의 초기 운전특성

이승란,김도한,나영수,이창한,박영식,윤태경,송승구 한국환경과학회 2002 한국환경과학회지 Vol.11 No.3

The lab-scale anaerobic continuous reactor which was filled with the sludge of anaerobic digestion from Suyoung wastewater treatment plant was operated by feeding of various concentrations and flow rates. This experiment indicated that more than 6,870 ㎎COD/L of substrate concentration was required to promote good metabolism and growth of anaerobic biomass. And increasing loading rate slowly was also required in order to treat substrate of higher concentration and higher loading rate. The substrate concentration of about 10,000 ㎎COD/L was adequate to generate biogas efficiently. The pH was sharply decreased at the onset of higher loading rate, but the pH was restabilized soon at 8. During the experiment, the amount of the attached biomass was kept constant.

폴리에틸렌 담체에 부착된 협기성 생물막 부착 특성

이승란,김도한,나영수,이창한,박영식,윤태경,송승구 한국환경과학회 2002 한국환경과학회지 Vol.11 No.4

Optical microscope, SEM (Scanning Electron Microscopy) and fluorescent microscope were used for qualitative and morphological studies of the attached biomass on PE (polyethylene) substratum under anaerobic condition. It was shown by the observation of optical microscope that the initial attachment of biomass began in crevices of the surface of PE. The shape and structure of the attached biofilm could be observed by SEM photographs, but species of bacteria were and methanogens were not classified. A large number of methanogenic bacteria were identified on the surface of PE substratum by fluorescence under 480㎚ of radiation. It was estimated that methanogenic bacteria was also related to initial attachment of biomass under anaerobic condition.