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OsAMT1.3 expression alters rice ammonium uptake kinetics and root morphology
Leandro Martins Ferreira,Vinicius Miranda de Souza,Orlando Carlos Huertas Tavares,Everaldo Zonta,Claudete Santa-Catarina,Sonia Regina de Souza,Manlio Silvestre Fernandes,Leandro Azevedo Santos 한국식물생명공학회 2015 Plant biotechnology reports Vol.9 No.4
High-affinity ammonium transporters (AMT1) are responsible for ammonium (NH4 ?) acquisition and/or perception in the micromolar range, and their expressions can be differentially regulated by nitrogen (N) availability. The present study characterised the functions of the rice (Oryza sativa) OsAMT1.3 transporter to understand its contribution to NH4+ acquisition and plant adaptation to environments with low N availability. Transgenic rice plants were obtained to study the activity of the OsAMT1.3 promoter (POsAMT1.3:GFP:GUS) and the overexpression of the OsAMT1.3 gene (UBIL:OsAMT1.3:3xHA) in plants. The OsAMT1.3 promoter activity was induced strongly in the absence of N and occurred primarily in the zones of lateral root emission and root tips. Anatomical sections of the segment of root tips and the middle third showed a differential pattern of OsAMT1.3 activity. Analysis of the OsAMT1.1–1.3 transporter expression profiles indicated that overexpression of OsAMT1.3 positively affected OsAMT1.2 expression. When subjected to a low N supply, plants overexpressing OsAMT1.3 showed lower KM and Cmin values. Additionally, these lines showed longer roots with a higher area, volume, and number of tips. The data suggested that OsAMT1.3 is involved in the ability of rice plants to adapt to low NH4+ supplies.
OsDof25 expression alters carbon and nitrogen metabolism in Arabidopsis under high N-supply
Leandro Azevedo Santos,Sonia Regina de Souza,Manlio Silvestre Fernandes 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
To verify the effect of the transcription factor OsDof25 on the nitrogen metabolism in plants, it was cloned for expression in Arabidopsis thaliana under the control of the 35S promoter. Lineages with different expression levels of this gene were obtained, and when grown in a half-strength MS medium (10 mM of NH4? and 20 mM of NO3-) showed phenotypes with visible chloroses and growth disorders. It was observed that OsDof25increased the levels of expression of high and low affinity ammonium transporters (AtAMT1.1 and AtAMT2.1,respectively) and repressed the high affinity nitrate transporter (AtNRT2.1). We also verified an increase in total amino-N content and expressions of the pyruvate kinase (PK1 and PK2), phosphoenolpyruvate carboxylase (PEPC1and PEPC2) and NADP-dependent and NAD-dependent isocitrate dehydrogenase. In addition, an increase in expression levels and activity of glutamate dehydrogenase was also observed. The simultaneous increase in the expression of AMTs and enzymes of carbon metabolism may explain the high levels of amino-N found in the transgenic plants. The data found in this work suggest that OsDof25 expression simultaneously affects NH4? uptake and organic acid metabolism in plants.
Leandro L. de Souza,Fernanda L.B. de Araujo,Fernanda A.M. da Silva,Thaís S. Mucciaroni,João E. de Araujo 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.5
We previously showed that a yin meridian of the upper limb decreased electromyographic activity (root mean square) and muscle strength ipsilateral and contralateral to the side of stimulation. Here, we tested the upper trapezius (UT) muscle response after stimulation of a yang meridian of the upper limb, the small intestine (SI). Thirty-eight healthy volunteers were randomized into the following groups: UT muscle (SI14), distant of the UT muscle (SI8), without stimulation (CG), and sham (R3). An acupuncturist certificated by the Brazilian Society of Physical Therapists and Acupuncturists performed the needle insertion. Each volunteer received only one stimulation to the right upper limb. The evaluation occurred before, 5 minutes after, and 20 minutes after needle withdrawal. The root mean square activity increased on the right side in the UT muscle for the SI8 and SI14 groups (F3,37 = 4.67; p < 0.025) at the 20-minute evaluation. The most vigorous response occurred on the contralateral side because the effects were maintained for 5 minutes after withdrawal (F3,37 = 4.52; p < 0.025). Both groups showed an increase in the UT muscle strength at the 20-minute evaluation (F3,37 = 3.41;p < 0.025). The CG and R3 groups did not show any changes. Our data indicate that SI a yang meridian increases the UT muscle response.
Rates of Chlorimuron Applied in Glyphosate-Tolerant and Sulfonylurea-Tolerant Soybean
Leandro Paiola Albrecht,Alfredo Junior Paiola Albrecht,André Felipe Moreira Silva,Fábio Henrique Krenchinski,Henrique Fabrício Placido,Ricardo Victoria Filho 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.3
Susceptibility to chlorimuron varies according to soybean genotype. STS® (sulfonylurea-tolerant soybean) presents a high tolerance to some sulfonylureas; this feature is determined by semi-dominants alleles, Als1 and Als2. Experiments were conducted in the field for four seasons with two cultivars for season to evaluate the selectivity of chlorimuron rates in post-emergency (V4) of glyphosate-tolerant and sulfonylurea-tolerant soybean. Data analysis made it possible to infer that there was no significant effect on the productivity of the cultivars when they received application in post-emergency, of chlorimuron at the doses used (0, 15, 30, 45, 60, 75, and 90 g ai ha-1). The maximum recommended rate for non-STS cultivars of chlorimuron is 20 g ai ha-1, or 4.5 times less than the maximum rate employed in this study. The soybean cultivars CD 250 RR/STS, CD 236 RR/STS, CD 2630 RR/STS, and BMX Turbo RR/STS were tolerant to the application, in post-emergency (V4) of the herbicide chlorimuron, until the rate of 90 g ai ha-1.
Leandro C. Luiz,Francisco J.O. Ferreira,Verginia R. Crispim 한국원자력학회 2017 Nuclear Engineering and Technology Vol.49 No.4
The presence of crust on the inner walls of metallic ducts impairs transportation becausecrust completely or partially hinders the passage of fluid to the processing unit and causesdamage to equipment connected to the production line. Its localization is crucial. With thedevelopment of the electronic imaging system installed at the Argonauta/Nuclear EngineeringInstitute (IEN)/National Nuclear Energy Commission (CNEN) reactor, it becamepossible to visualize crust in the interior of metallic piping of small diameter using realtimeneutron radiography images obtained with a low neutron flux. The obtained imagesshowed the resistance offered by crust on the passage of water inside the pipe. Nodiscrepancy of the flow profile at the bottom of the pipe, before the crust region, wasregistered. However, after the passage of liquid through the pipe, images of the disturbancesof the flow were clear and discrepancies in the flow profile were steep. This showsthat this technique added the assembled apparatus was efficient for the visualization ofthe crust and of the two-phase flows.
Cyber security of critical infrastructures
Leandros A. Maglaras,김기형,Helge Janicke,Mohamed Amine Ferrag,Stylianos Rallis,Pavlina Fragkou,Athanasios Maglaras,Tiago J. Cruz 한국통신학회 2018 ICT Express Vol.4 No.1
Modern Supervisory Control and Data Acquisition (SCADA) systems are essential for monitoring and managing electric power generation, transmission and distribution. In the age of the Internet of Things, SCADA has evolved into big, complex and distributed systems that are prone to be conventional in addition to new threats. Many security methods can be applied to such systems, having in mind that both high efficiency, real time intrusion identification and low overhead are required.
Leandro Fernandes Andrade,Gleika Larisse Oliveira Dorasio de Souza,Silvia Nietsche,Adelica Aparecida Xavier,Marcia Regina Costa,Acleide Maria Santos Cardoso,Marlon Cristian Toledo Pereira,Débora Franc 한국미생물학회 2014 The journal of microbiology Vol.52 No.1
A total of 40 endophytic bacterial isolates obtained from banana tree roots were characterized for their biotechnological potential for promoting banana tree growth. All isolates had at least one positive feature. Twenty isolates were likely diazotrophs and formed pellicles in nitrogen-free culture medium, and 67% of these isolates belonged to the genus Bacillus sp. The isolates EB-04, EB-169, EB-64, and EB-144had N fixation abilities as measured by the Kjeldahl method and by an acetylene reduction activity assay. Among the 40isolates, 37.5% were capable of solubilizing inorganic phosphate and the isolates EB-47 and EB-64 showed the highest solubilization capacity. The isolate EB-53 (Lysinibacillus sp.)had a high solubilization index, whereas 73% of the isolates had low solubilization indices. The synthesis of indole-3-acetic acid (IAA) in the presence of L-tryptophan was detected in 40% of the isolates. The isolate EB-40 (Bacillus sp.)produced the highest amount of IAA (47.88 μg/ml) in medium supplemented with L-tryptophan and was able to synthesize IAA in the absence of L-tryptophan. The isolates EB-126 (Bacillus subtilis) and EB-47 (Bacillus sp.) were able to simultaneously fix nitrogen, solubilize phosphate and produce IAA in vitro. The results of this study demonstrated that the isolates analyzed here had diverse abilities and all have the potential to be used as growth-promoting microbial inoculants for banana trees.
Leandro Papinutti,Flavia Forchiassin 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6
We investigated the use of solid residues from Pleurotus ostreatus mushroom production in adsorbing and decolorizing different dyes. The solid residue used in this study was composed of hemicellulose and cellulose (52.81%), acid-insoluble lignin (25.42%), chitin (6.5%), and water extractives (14.82%). After incubating 14% (wt/vol)solid residue in distilled water for 4 h, laccase and manganese peroxidase (MnP) activities were 0.5 U/g and 12 mU/g, respectively. Enzymatic decolorization percentages were up to 100 for azure B (heterocyclic dye) and indigo carmine (indigoid dye), 74.5 for malachite green (MG) (triphenylmethane dye), and zero for xylidine (azoic dye). The optimum temperature for decolorization was in the range of 26 ~ 36ºC for all dyes. Data obtained on adsorption (enzymatic decolorization was prevented with sodium azide) at different dye concentrations and in a pH range of 3 ~ 7 were used to plot Freundlich isotherms. The spent fungal substrate (SFS) displayed large differences in adsorption capacity, depending on the dye tested. The highest adsorption capacity was observed at pH 3 for MG,while xylidine was slightly adsorbed at pH 3 and 4 and not adsorbed at higher pH values. Laccase and MnP production were affected by the presence of the dyes. The highest enzyme levels were observed in the presence of MG, when laccase and MnP increased 1.39- and 2.13-fold, respectively. Decolorization and adsorption to SFS are both important processes in removing dyes from aqueous solutions. The application of this spent substrate for wastewater treatment will be able to take advantage of both of these dye removal processes. An important problem in bioremediation processes involving microorganisms is the amount of time required for their growth. In this report, we used the spent substrates from mushroom cultivation in wastewater treatment, thus solving the problem of waiting for microorganisms to grow.
Leandro Freire dos Santos,Ana Lucia Zanatta,Vanete Thomaz Soccol,Maria Fernanda Torres,Sandro José Ribeiro Bonatto,Rosália Rubel,Carlos Ricardo Soccol 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.1
The ability of Pleurotus ostreatus biomass,cultived by submerged fermentation, to produce beneficial effect on lipid profile and macrophages activity during a high-fat diet (HFD) for a long-term intake was investigated. Blood samples were collected through cardiac puncture to measure the plasma cholesterol, triglycerides, low-density protein (LDL), high-density protein (HDL), aspartate aminotransferase (AST) activity, urea– blood urea nitrogen (BUN)/creatinine ratio of rats fed on an HFD for 4 months. Dosage of lipid hydroperoxides was carried out on methanolic extract of liver tissue. Peritoneal macrophages activity was evaluated in relation to the superoxide anion,hydrogen peroxide and nitric oxide production, phagocytosis and lysosomal volume. The administration of P. ostreatus significantly altered the lipid profile and oxidative stress as related to the LDL and triglycerides decrease and inhibitory effects on superoxide anion and hydrogen peroxide production. All findings of this study lead us to suggest that the P. ostreatus maybe a beneficial agent in the hyperlipidemia and atherosclerosis treatments.