The Genetic Relatedness Analysis of Male Sterile and Their Maintainer Lines of Onion (Allium cepa L.) by Using RAPD Primers

Lakshmana Reddy Dhoranalapalli,Dhanya shivnanajappa,Verre Gowda,Sreedhara Sindughatta Antharamiah,Aswath Chennareddy 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.1

RAPD markers analysis was performed to investigate genetic relatedness among nine sterile (A), maintainer lines (B), and male parents (C) of onion. Initially, 180 arbitrary decamer primers were screened; of these 20 primers were selected which yielded 812bands, 45.34% of which were found to be polymorphic. Squared-Euclidean Distance matrix revealed a minimum genetic distance between the genotypes MS 48A and MF 65C and a maximum genetic distance between the genotypes MLT 84C and MF 65B. Cluster analysis grouped the nine onion genotypes into two major clusters. The first cluster consisted of two male sterile groups, i.e. MS-65 (Arka Lalima) and MS-48 (Arka Kirthiman) of single bulb onions. The second cluster consists of MLT (Multiplier) group. PCA re-confirmed the results of the dendrogram, revealing the precision in estimation of genetic relatedness among the genotypes tested. None of the primers produced unique banding patterns to distinguish all nine genotypes. This information about genetic relatedness among the nine genotypes would be better utilized in further onion breeding programs

SWEFT: Semantic Web Service Engine for Telecommunication – An Automatic Discovery and Composition through Genetic Approach

Lakshmana Kumar Ramasamy,M.S.Irfan Ahmed 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.9

The web service developed by telecommunication domain is ineffective since they are demonstrated by syntactic description rather than semantic. The motivation of the research is to have semantic description with existing web services, and provides discovery, composition and invocation of web services automatically. The objective is to identify the discovery and composition concerns and devise a compositional approach that covers all concerns. So a new prototype named Semantic Web Service Engine for Telecommunication which automatically discover and composite a web service was proposed, enables semantic through upper ontology and maps Web Service Description Language to Ontology Web Language-Semantic. For composition, a genetic algorithm was proposed which can solve problems with great distinctiveness. This approach automatically discovers and generates the required composite semantic web services and considers all identified concerns concurrently, improves the accuracy for the service discovery and unifies the semantic representation of telecommunications without human intervention.

Novel role of RanBP9 in BACE1 processing of amyloid precursor protein and amyloid beta peptide generation.

Lakshmana, Madepalli K,Yoon, Il-Sang,Chen, Eunice,Bianchi, Elizabetta,Koo, Edward H,Kang, David E American Society for Biochemistry and Molecular Bi 2009 The Journal of biological chemistry Vol.284 No.18

<P>Accumulation of the amyloid beta (Abeta) peptide derived from the proteolytic processing of amyloid precursor protein (APP) is the defining pathological hallmark of Alzheimer disease. We previously demonstrated that the C-terminal 37 amino acids of lipoprotein receptor-related protein (LRP) robustly promoted Abeta generation independent of FE65 and specifically interacted with Ran-binding protein 9 (RanBP9). In this study we found that RanBP9 strongly increased BACE1 cleavage of APP and Abeta generation. This pro-amyloidogenic activity of RanBP9 did not depend on the KPI domain or the Swedish APP mutation. In cells expressing wild type APP, RanBP9 reduced cell surface APP and accelerated APP internalization, consistent with enhanced beta-secretase processing in the endocytic pathway. The N-terminal half of RanBP9 containing SPRY-LisH domains not only interacted with LRP but also with APP and BACE1. Overexpression of RanBP9 resulted in the enhancement of APP interactions with LRP and BACE1 and increased lipid raft association of APP. Importantly, knockdown of endogenous RanBP9 significantly reduced Abeta generation in Chinese hamster ovary cells and in primary neurons, demonstrating its physiological role in BACE1 cleavage of APP. These findings not only implicate RanBP9 as a novel and potent regulator of APP processing but also as a potential therapeutic target for Alzheimer disease.</P>

INFLUENCE OF DAMPING ON THE OSCILLATORY RESPONSE OF A CIRCULAR CYLINDER DUE TO INTERFERENCE EFFECTS

B .H. Lakshmana Gowda,V. Sreedharan 대한기계학회 1992 International Conference on Fluid Machinery Vol.1 No.1

Mitochondrial dysfunction and calcium deregulation by the RanBP9-cofilin pathway

Roh, Seung-Eon,Woo, Jung A.,Lakshmana, Madepalli K.,Uhlar, Courtney,Ankala, Vinishaa,Boggess, Taylor,Liu, Tian,Hong, Yun-Hwa,Mook-Jung, Inhee,Kim, Sang Jeong,Kang, David E. The Federation of American Societies for Experimen 2013 The FASEB Journal Vol.27 No.12

<P>Mitochondrial dysfunction and synaptic damage are important features of Alzheimer's disease (AD) associated with amyloid β (Aβ) and tau. We reported previously that the scaffolding protein RanBP9, which is overall increased in brains of patients with AD and in mutant APP transgenic mice, simultaneously promotes Aβ generation and focal adhesion disruption by accelerating the endocytosis of APP and β1-integrin, respectively. Moreover, RanBP9 induces neurodegeneration <I>in vitro</I> and <I>in vivo</I> and mediates Aβ-induced neurotoxicity. Here we show in primary hippocampal neurons that RanBP9 potentiates Aβ-induced reactive oxygen species (ROS) overproduction, apoptosis, and calcium deregulation. Analyses of calcium-handling measures demonstrate that RanBP9 selectively delays the clearance of cytosolic Ca<SUP>2+</SUP> mediated by the mitochondrial calcium uniporter through a process involving the translocation of cofilin into mitochondria and oxidative mechanisms. Further, RanBP9 retards the anterograde axonal transport of mitochondria in primary neurons and decreases synaptic mitochondrial activity in brain. These data indicate that RanBP9, cofilin, and Aβ mimic and potentiate each other to produce mitochondrial dysfunction, ROS overproduction, and calcium deregulation, which leads to neurodegenerative changes reminiscent of those seen in AD.—Roh. S.-E., Woo, J. A., Lakshmana, M. K., Uhlar, C., Ankala, V., Boggess, T., Liu, T., Hong, Y.-H., Mook-Jung, I., Kim, S. J., Kang, D. E. Mitochondrial dysfunction and calcium deregulation by the RanBP9-cofilin pathway.</P>

Techniques for dental implant nanosurface modifications

Preeti Pachauri,Lakshmana Rao Bathala,Rajashekar Sangur 대한치과보철학회 2014 The Journal of Advanced Prosthodontics Vol.6 No.6

PURPOSE. Dental implant has gained clinical success over last decade with the major drawback related to osseointegration as properties of metal (Titanium) are different from human bone. Currently implant procedures include endosseous type of dental implants with nanoscale surface characteristics. The objective of this review article is to summarize the role of nanotopography on titanium dental implant surfaces in order to improve osseointegration and various techniques that can generate nanoscale topographic features to titanium implants. MATERIALS AND METHODS. A systematic electronic search of English language peer reviewed dental literature was performed for articles published between December 1987 to January 2012. Search was conducted in Medline, PubMed and Google scholar supplemented by hand searching of selected journals. 101 articles were assigned to full text analysis. Articles were selected according to inclusion and exclusion criterion. All articles were screened according to inclusion standard. 39 articles were included in the analysis. RESULTS. Out of 39 studies, seven studies demonstrated that bone implant contact increases with increase in surface roughness. Five studies showed comparative evaluation of techniques producing microtopography and nanotopography. Eight studies concluded that osteoblasts preferably adhere to nano structure as compared to smooth surface. Six studies illustrated that nanotopography modify implant surface and their properties. Thirteen studies described techniques to produce nano roughness. CONCLUSION. Modification of dental osseous implants at nanoscale level produced by various techniques can alter biological responses that may improve osseointegration and dental implant procedures.

A Survey on Detection and Prevention of Cross-Site Scripting Attack

V. Nithya,S. Lakshmana Pandian,C. Malarvizhi 보안공학연구지원센터 2015 International Journal of Security and Its Applicat Vol.9 No.3

In present-day time, securing the web application against hacking is a big challenge. One of the common types of hacking technique to attack the web application is Cross-Site Scripting (XSS). Cross-Site Scripting (XSS) vulnerabilities are being exploited by the attackers to steal web browser’s resources such as cookies, credentials etc. by injecting the malicious JavaScript code on the victim’s web applications. Since Web browsers support the execution of commands embedded in Web pages to enable dynamic Web pages attackers can make use of this feature to enforce the execution of malicious code in a user’s Web browser. The analysis of detection and prevention of Cross-Site Scripting (XSS) help to avoid this type of attack. We describe a technique to detect and prevent this kind of manipulation and hence eliminate Cross-Site Scripting attack.

Does computer-aided diagnostic endoscopy improve the detection of commonly missed polyps? A meta-analysis

Arun Sivananthan,Scarlet Nazarian,Lakshmana Ayaru,Kinesh Patel,Hutan Ashrafian,Ara Darzi,Nisha Patel 대한소화기내시경학회 2022 Clinical Endoscopy Vol.55 No.3

Background/Aims: Colonoscopy is the gold standard diagnostic method for colorectal neoplasia, allowing detection and resection ofadenomatous polyps; however, significant proportions of adenomas are missed. Computer-aided detection (CADe) systems in endoscopyare currently available to help identify lesions. Diminutive (≤5 mm) and nonpedunculated polyps are most commonly missed. This meta-analysis aimed to assess whether CADe systems can improve the real-time detection of these commonly missed lesions. Methods: A comprehensive literature search was performed. Randomized controlled trials evaluating CADe systems categorized bymorphology and lesion size were included. The mean number of polyps and adenomas per patient was derived. Independent proportionsand their differences were calculated using DerSimonian and Laird random-effects modeling. Results: Seven studies, including 2,595 CADe-assisted colonoscopies and 2,622 conventional colonoscopies, were analyzed. CADe-assistedcolonoscopy demonstrated an 80% increase in the mean number of diminutive adenomas detected per patient compared withconventional colonoscopy (0.31 vs. 0.17; effect size, 0.13; 95% confidence interval [CI], 0.09−0.18); it also demonstrated a 91.7% increasein the mean number of nonpedunculated adenomas detected per patient (0.32 vs. 0.19; effect size, 0.05; 95% CI, 0.02−0.07). Conclusions: CADe-assisted endoscopy significantly improved the detection of most commonly missed adenomas. Although thismethod is a potentially exciting technology, limitations still apply to current data, prompting the need for further real-time studies.

Risk and resilience factors for substance use among street adolescents: Assessment and development of an integrative model

Ramaswamy Dhanasekara Pandian,Govindappa Lakshmana 한국사회복지학회 2017 Asian Social Work and Policy Review Vol.11 No.3

In this study, risk and protective factors for substance use were explored to develop an intervention program and test its efficacy among male street-based adolescents. Twenty expert interviews and key informant interviews were conducted. In addition, 30 street adolescents (15 substance-users and 15 non-users) participated in four focus group discussions for needs assessment, wherein risk and protective factors were discussed. Based on the identified needs, an intervention package was developed and validated by experts. The package was implemented in a natural environment of 50 street adolescents. After the intervention, about 60% of the respondents quit or cut down on their substance use substantially, and approximately 40% of the respondents agreed to reunite with their respective families, while 20% did not change their substance use habits. The results showed that if several protective factors were present, there was a lower risk of substance use. Many factors contribute to substance use and protective factors need to be strengthened in order to make interventions effective.

Distribution of Serotypes and Antibiotic Susceptibility Patterns Among Invasive Pneumococcal Diseases in Saudi Arabia

Yazeed A. Al-Sherikh,Lakshmana K. Gowda,M. Marie Mohammed Ali,James John,Dabwan Khaled Homoud Mohammed,Pradeep Chikkabidare Shashidhar 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.3

Background: Streptococcus pneumoniae causes life-threatening infections such as men- ingitis, pneumonia, and febrile bacteremia, particularly in young children. The increasing number of drug-resistant isolates has highlighted the necessity for intervening and control- ling disease. To achieve this, information is needed on serotype distribution and patterns of antibiotic resistance in children. Methods: All cases of invasive pneumococcal disease (IPD) in children aged less than 15 yr recorded at King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia, were reviewed for serotyping and antibiotic susceptibility. Isolates were collected from 78 consecutive patients with IPD between 2009 and 2012. All collected isolates were sub- jected to serotyping by co-agglutination, sequential multiplex PCR, and single PCR se- quetyping as previously described. Results: The most frequently isolated IPD serotypes were 23F, 6B, 19F, 18C, 4, 14, and 19A, which are listed in decreasing order and cover 77% of total isolates. The serotype coverage for the pneumococcal conjugate vaccine (PCV)7, PCV10, and PCV13 was 77%, 81%, and 90%, respectively. Results from sequential multiplex PCR agreed with co-agglu- tination results. All serotypes could not be correctly identified using single PCR sequetyp- ing. Minimum inhibitory concentration showed that 50 (64%) isolates were susceptible to penicillin, whereas 70 (90%) isolates were susceptible to cefotaxime. Conclusions: The most common pneumococcal serotypes occur with frequencies similar to those found in countries where the PCV has been introduced. The most common sero- types in this study are included in the PCVs. Addition of 23A and 15 to the vaccine would improve the PCV performance in IPD prevention.