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      • 활동기 척추동물의 십이지장 배상세포내 점액질에 관한 조직화학적 연구

        박래백,한현주,임영빈,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

        본 실험은 다람쥐(Tamias sibiricus asiaticus Gmeli), 살모사(Agkistrodon halys Pallas), 황소개구리(Rana catesbeiana Shaw), 뱀장어(Anguilla japonica Temminck et Schlegel)등의 소장 배상세포내 점액질에 대한 성상을 밝히기 위하여 PAS reaction, Alcian blue pH2.5 및 Alcian blue pH0.4, 그리고 ABpH2.5-PAS reaction 복합염색등을 시행한 결과 다음과 같은 결론을 얻었다. 1. 사용한 각 동물에 있어서 소장 배상세포는 중성점액질, 약산성 및 강산성 황화점액질을 포함하고 있었다. 2. 중성점액질이 각 동물에 있어서 가장 안정된 분포를 보였으며 약산성 황화점액질도 비교적 고르게 분포되어 있었다. 3. 강산성 황화점액질은 다람쥐, 살모사 및 황소개구리에서는 소량 관찰되어 불안정한 분포를 보였으며 뱀장어에서는 관찰되지 않았다. 4. 각 실험동물의 십이지장 배상세포내에 포함된 점액질의 성상은 동물에 따라서 그 양과 염색성에 다소의 차이를 인정할 수 있다. In general, the goblet cells in the small intestines of experimental animals contained neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances. Neutral mucosubstances show most stable distribution in the experimental animals. Mucosubstances in the small intestines of eels were composed of only neutral mucosubstances and weakly acidic sulfated mucosubstances. The contents and stain properties of mucostances produced in the intestinal goblet cells vary a little in portions within a species and in the same cell types of different species. This studies were performed in order to study composition of the mucosubstance in the goblet cells of small intestines of the various vertebrates. The specimens used in this study were obtained from the small intestines of 4 species of vertebrates, squirrels (Tamias sibiricus asiaticus Gmelin), vipers (Agkistrodon halys Palls), frogs (Rana catesbeiana Shaw), eels (Anguilla japonica Temminck et Scchlegel). All specimens were fixed in 10% neutral buffered formalin, embedded in paraffin wax, and sectioned at a thickness of 4㎛. They were stained with hematoxylin-eosin for general histological observation and with Periodic Acid-Schiff reaction, alcian blue pH2.5 and 0.4 and alcian blue pH2.5-Periodic Acid-Schiff reaction for detection of the mucosubstances. The mucous compositions in the goblet cells of the small intestines of various vertebrates examined by their staining reaction were neutral mucosubstances, weakly and strongly acidic sulfated mucosubstances.

      • 활동기와 동면기 살모사의 위장관 점액세포내 점액질에 관한 조직화학적 연구

        한현주,박래백,임영민,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

        살모사(Aekistrodon halys Pallas)의 위장관 점액세포내 점액질을 활동기와 동면기로 나누어 조직화학적으로 비교관찰하였다. 일반관찰을 위하여 H-E 염색을, 조직화학적 소견을 관찰하기 위하여 PAS reaction, Ab2.5 염색, AB1.0염색, AB2.5-PAS 염색, AB1.0-PAS 염색 AF1.7 염색 및 AF1.7-AB2.5 염색등을 시행하였다. 위(胃)는 표면점액세포와 점액경세포의 점액질을, 소장은 은롸롸 융모의 점액세포를 관찰한 결과를 다음과 같이 요약하였다. 1. 활동기와 동면기의 위점막에서는 현저한 차이는 없으나, 소장점막은 동면기에는 점막 주름수가 감소하고 높이가 낮아지며 점액세포가 위축되었다. 2. 위 표면점액세포는 활동기에 중성점액질과 약간의 Sialomucin을 포함한 산성점액질로 구성되었으나, 동면기에는 중성점액질과 Sialomucin이 약간 감소하였다. 3. 위 점액경세포는 활동기에 중성점액질만을 포함하고 있었으나, 동면기에는 Sialomucin을 포함한 산성점액질이 소량 검출되었다. 4. 소장 점액세포는 활동기에 산성점액질과 중성점액질로 구성되었으며 산성점액질에는 Sulfomucin과 Sialomucin이 포함되어 있었으나, 동면기에는 Sulfomucin과 Sialomucin이 다소 감소하였다. This study was performed in order to investigate the histochemical properties and compositions of the mucosubstances in the mucous of the gastrointestinal tract of Vipers, Agkistrodon halys Pallas, in active and hibernating phases. The specimens from stomach and small intestine were fixed in 10% neutral buffered formalin, embedded in paraffin wax, sectioned at a thickness of 4㎛, and stained with Hematoxyling-eosin for general histological observation and with PAS reaction, Alcian blue pH 2.5 or 1.0, Alcian blue pH 2.5-PAS or 1.0-PAS reaction, Aldehyde fuchsin pH 1.7, and Aldeliyde fuchsin pH 1.7-Alcian Blue pH 2.5 for histochemical finding. The mucous compositions in mucous cells of the gastrointestinal tract examined by their stain reaction were neutral mucin, acidic mucin, sulfomucin, sialomucin, The results obtained were as follows; The folds of the gastrointestinal tract in the hibernating phase, compared with the active phase, shrink and lower. Also, the shrinkage of epithelial cells were observed in the hibenating phase. The surface mucous cells of the stomach in the active phase composed mainly neutral mucin and a little amount of acidic mucin including sialomucin, but in the hibernating phase neutral mucin and sialomucin slightly decreased. The mucous neck cells of the stomach in the active phase constituted mainly neutral mucin, In the hebernating phase the mucous neck cells were composed of neutral mucin and a little amount of acidic mucin contained sialomucin. The mucous cells of the small intestine in the active phase consist of neutral mucin, acidic mucin including sulfomucin and sialomucin but in the hibernating phase sulfomucin and sialomucin slightly decreased.

      • 동면동물 혈액상의 생리적 변화에 관한 연구

        임영민,한현주,박래백,김효성,박경수,노영복 조선대학교 기초과학연구소 1990 自然科學硏究 Vol.13 No.1

        활동기와 동면기에 동면동물인 살모사에 있어서 혈액내 가스함량과 부분압인 TCO_2, P0_2, PO_2 및 pH와 일반적인 혈액상인 적혈구수, 백혈구수, 혈색소, Hematocrit 등의 변화를 조사한바 다음과 같이 요약하였다. 1. 총이산화탄소량 (TCO_2), 이산화탄소부분압P(C0_2), 산소압(PO_2)과 pH는 활동기보다 동면기 에 증가하였다. 2. 적혈구수, 혈색소, 적혈구백분율(Hematocrit)은 활동기보다 동면기에 약간 증가하는 경향이었다. 3. 백혈구수는 활동기보다 동면기에 뚜렷한 감소를 보였다. 환경온도와 체온의 저하로 인한 혈액내의 변화는 인정할 수 있었다. This thesis were performed in order to study physiological changes of vipers in the active phase and hibernation phase, Physiological changes were blood gas volume and pressure (TCO_2, PCO_2, PO_2), blood pH and blood pictures (RBC, WBC, Hemoglobin, Hematocrit) The obtained results were summalize as follows; 1. TCO_2, PCO_2, PO_2 and pH increased considerably in hibernation phase, compared with active phase. 2. RBC, Hemoglobin and Hematocrit increased slightly in hibernation phase, compared with active phase. 3. WBC, decreased considerably in hibernation phase, compared with active phase 4. The decrease of environmental and body temperature showed change of blood components.

      • KCI등재

        Morphology and Molecular Characterization of a Fungus from the Alternaria alternata Species Complex Causing Black Spots on Pyrus sinkiangensis (Koerle pear)

        ( Sein Lai Lai Aung ),( Hai Feng Liu ),( Dong Fang Pei ),( Bing Bin Lu ),( May Moe Oo ),( Jian Xin Deng ) 한국균학회 2020 Mycobiology Vol.48 No.3

        A small-spored Alternaria was found from black spots of storaged Koerle pear (Pyrus sinkiangensis), one of the economically important fruit in Xinjiang province, China. The morphology is similar to A. limoniasperae but obviously different in secondary conidiophores and conidial septa. A phylogenetic analysis using sequence datasets of ITS, GAPDH, TEF1, RPB2, Alt a1, OPA10-2, and EndoPG genes revealed that it belonged to the Alternaria alternata complex group. Pathogenicity tests illustrated that the fungus was the causal pathogen of black spot on Koerle pear fruit.

      • SCIESCOPUSKCI등재

        A Tracking System Using Location Prediction and Dynamic Threshold for Minimizing SMS Delivery

        Lai, Yuan-Cheng,Lin, Jian-Wei,Yeh, Yi-Hsuan,Lai, Ching-Neng,Weng, Hui-Chuan The Korea Institute of Information and Commucation 2013 Journal of communications and networks Vol.15 No.1

        In this paper, a novel method called location-based delivery (LBD), which combines the short message service (SMS) and global position system (GPS), is proposed, and further, a realistic system for tracking a target's movement is developed. LBD reduces the number of short message transmissions while maintaining the location tracking accuracy within the acceptable range. The proposed approach, LBD, consists of three primary features: Short message format, location prediction, and dynamic threshold. The defined short message format is proprietary. Location prediction is performed by using the current location, moving speed, and bearing of the target to predict its next location. When the distance between the predicted location and the actual location exceeds a certain threshold, the target transmits a short message to the tracker to update its current location. The threshold is dynamically adjusted to maintain the location tracking accuracy and the number of short messages on the basis of the moving speed of the target. The experimental results show that LBD, indeed, outperforms other methods because it satisfactorily maintains the location tracking accuracy with relatively fewer messages.

      • Evidence of Tandem Repeat and Extra Thiol-groups Resulted in the Polymeric Formation of Bovine Haptoglobin: A Unique Structure of Hp 2-2 Phenotype

        Lai, Yi An,Lai, I Hsiang,Tseng, Chi Feng,Lee, James,Mao, Simon J.T. Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.6

        Human plasma Hp is classified as 1-1, 2-1, and 2-2. They are inherited from two alleles Hp 1 and Hp 2, but there is only Hp 1 in almost all the animal species. Hp 2-2 molecule is extremely large and heterogeneous associated with the development of inflammatory-related diseases. In this study, we expressed entire bovine Hp in E. coli as a $\alpha\beta$ linear form. Interestingly, the antibodies prepared against this form could recognize the subunit of native Hp. In stead of a complicated column method, the antibody was able to isolate bovine Hp via immunoaffinity and gelfiltration columns. The isolated Hp is polymeric containing two major molecular forms (660 and 730 kDa). Their size and hemoglobin binding complex are significantly larger than that of human Hp 2-2. The amino-acid sequence deducted from the nucleotide sequence is similar to human Hp 2 containing a tandem repeat over the $\alpha$ chain. Thus, the Hp 2 allele is not unique in human. We also found that there is one additional -SH group (Cys-97) in bovine $\alpha$ chain with a total of 8 -SH groups, which may be responsible for the overall polymeric structure that is markedly different from human Hp 2-2. The significance of the finding and its relationship to structural evolution are also discussed.

      • KCI등재

        L-Theanine-Treated Adipose-Derived Mesenchymal Stem Cells Alleviate the Cytotoxicity Induced by N-Nitrosodiethylamine in Liver

        Lai Yun-Ju,Sung Yi-Ting,Lai Yi-An,Chen Li-Nian,Chen Tung-Sheng,Chien Chiang-Ting 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.6

        BACKGROUND: Liver inflammation is the main cause of severe liver diseases, including liver fibrosis, steatohepatitis, cirrhosis and hepatocellular carcinoma. Cell therapy topics are receiving increasingly more attention. The therapeutic applications of mesenchymal stem cells (MSC) have become one of the most discussed issues. While other stem cells have therapeutic effects, they have only one or two clinical applications. MSCs are responsible for repairing a variety of tissue injuries. Moreover, MSCs could be derived from several sources, including adipose tissue. MSCs are usually more abundant and easier to obtain compared to other stem cells. METHODS: To prove the concept that MSCs have homing ability to the injured tissue and assist in tissue repair, we examined the effects of intravenous injected adipose-derived mesenchymal stem cells (ADSCs) in a N-nitrosodiethylamine (DEN)-induced liver injury rat model. RESULTS: The significant repairing ability of ADSCs was observed. The levels of fibrosis, apoptosis, and tumorigenesis in the DEN-injured liver tissues all decreased after ADSC treatment. Furthermore, to enhance the therapeutic effects of ADSCs, we pretreated them with L-theanine, which promotes the hepatocyte growth factor secretion of ADSC, and therefore improved the healing effects on injured liver tissue. CONCLUSION: ADSCs, especially L-theanine-pretreated ADSCs, have anti-inflammation, anti-apoptosis, and anti-tumorigenesis effects on the N-nitrosodiethylamine-induced liver injury rat model.

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