Effects of a soluble dietary fibre NUTRIOSE$^{(R)}$ on colonic fermentation and excretion rates in rats

Guerin-Deremaux, Laetitia,Ringard, Florence,Desailly, Fabrice,Wils, Daniel The Korean Nutrition Society 2010 Nutrition Research and Practice Vol. No.

The resistant dextrin NUTRIOSE$^{(R)}$, developed from starch, is expected to act as a prebiotic. The aim of this study was to determine the effects of NUTRIOSE$^{(R)}$ on cecal parameters, short-chain fatty acid (SCFA) concentrations, and fecal excretion in rats. In an initial experiment, twenty-four male Fischer F344 rats were randomly assigned to one of the following four treatments for 14 days: G0 (control diet), G2.5 (control diet+2.5% of dextrin), G5 (control diet + 5% of dextrin), and G10 (control diet + 10% of dextrin). After 14 days, total cecal weight, cecal content, and cecal wall weight were significantly increased in G5 and G10 compared to G0. At the same time, cecal pH was significantly lower in G10 compared to G0. Total SCFA concentration was significantly higher in G10 than in G5, G2.5, and G0, and significantly higher in G5 than in G0. Acetate, butyrate, and propionate concentrations were significantly increased in G5 and G10 compared to the controls. In a second trial based on a similar design, eighteen male Fischer F344 rats were treated with a control diet supplemented with 5% of dextrin or 5% of fructo-oligosaccharide. The results obtained with NUTRIOSE$^{(R)}$ were similar to those obtained with the fructo-oligosaccharide. In a third experiment, two groups of 5 Fischer F344 rats were orally treated with 100 and 1,000 mg/kg NUTRIOSE$^{(R)}$, respectively, and from 18% to 25% of the dextrin was excreted in the feces. The results of these three studies show that the consumption of NUTRIOSE$^{(R)}$, by its effects on total cecal weight, cecal content, cecal wall weight, pH, and SCFA production, could induce healthy benefits since these effects are reported to be prebiotic effects.

Effects of a soluble dietary fibre NUTRIOSE<SUP>®</SUP> on colonic fermentation and excretion rates in rats

Laetitia Guerin-Deremaux,Florence Ringard,Fabrice Desailly,Daniel Wils 한국영양학회 2010 Nutrition Research and Practice Vol.4 No.6

The resistant dextrin NUTRIOSE<SUP>®</SUP>, developed from starch, is expected to act as a prebiotic. The aim of this study was to determine the effects of NUTRIOSE<SUP>®</SUP> on cecal parameters, short-chain fatty acid (SCFA) concentrations, and fecal excretion in rats. In an initial experiment, twenty-four male Fischer F344 rats were randomly assigned to one of the following four treatments for 14 days: G0 (control diet), G2.5 (control diet + 2.5% of dextrin), G5 (control diet + 5% of dextrin), and G10 (control diet + 10% of dextrin). After 14 days, total cecal weight, cecal content, and cecal wall weight were significantly increased in G5 and G10 compared to G0. At the same time, cecal pH was significantly lower in G10 compared to G0. Total SCFA concentration was significantly higher in G10 than in G5, G2.5, and G0, and significantly higher in G5 than in G0. Acetate, butyrate, and propionate concentrations were significantly increased in G5 and G10 compared to the controls. In a second trial based on a similar design, eighteen male Fischer F344 rats were treated with a control diet supplemented with 5% of dextrin or 5% of fructo-oligosaccharide. The results obtained with NUTRIOSE<SUP>®</SUP> were similar to those obtained with the fructo-oligosaccharide. In a third experiment, two groups of 5 Fischer F344 rats were orally treated with 100 and 1,000 ㎎/㎏ NUTRIOSE<SUP>®</SUP>, respectively, and from 18% to 25% of the dextrin was excreted in the feces. The results of these three studies show that the consumption of NUTRIOSE<SUP>®</SUP>, by its effects on total cecal weight, cecal content, cecal wall weight, pH, and SCFA production, could induce healthy benefits since these effects are reported to be prebiotic effects.

Benefits of Preventive Administration of Chlorella sp. on Visceral Pain and Cystitis Induced by a Single Administration of Cyclophosphamide in Female Wistar Rat

Sophie Hidalgo-Lucas,Pascale Rozan,Laetitia Guerin-Deremaux,Blandine Baert,Nicolas Violle,Marie-Helene Saniez-Degrave,Jean-Francois Bisson 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.5

Chlorella sp. is a green microalgae containing nutrients, vitamins, minerals, and chlorophyll. In some communities, Chlorella sp. is a traditional medicinal plant used for the management of inflammation-related diseases. In a rat model, ROQUETTE Chlorella sp. (RCs) benefits were investigated on visceral pain and associated inflammatory parameters related to cystitis both induced by cyclophosphamide (CYP). RCs was orally administered every day from day 1–16 (250 and 500 mg/kg body weight). Six hours after an intraperitoneal injection of 200 mg/kg body weight of CYP, body temperature, general behavior, food intake, and body weight were recorded. Twenty-four hours after CYP injection, rats were tested in two behavioral tests, an open field and the aversive light stimulus avoidance conditioning test, to evaluate the influence of pain on general activity and learning ability of rats. After euthanasia, bladders were weighed, their thickness was scored, and the urinary hemoglobin was measured. RCs orally administered at the two dosages significantly reduced visceral pain and associated inflammatory parameters related to cystitis both induced by CYP injection, and improved rat behavior. To conclude, RCs demonstrated beneficial effects against visceral pain and cystitis.

A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats

김경아,유혜현,Wan Gu,Dae-Hyung Yu,Ming Ji Jin,Hae-Lim Choi,Kathy Yuan,Laetitia Guerin-Deremaux,김동현 고려인삼학회 2015 Journal of Ginseng Research Vol.39 No.2

Background: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K(CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, couldenhance the formation and consequent absorption of CK through the modulation of gut microbialmetabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics ofginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. Methods: Male SpragueeDawley rats were given control or NUTRIOSE-containing diets (controldiet þ NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood sampleswere collected to investigate the pharmacokinetics of CK using liquid chromatographyetandem massspectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimensor bacteria cultures. Results: When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSEcontaining diets, the maximum plasma concentration (Cmax) and area under the plasma concentrationetime curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSEintake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activitiesof intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. Conclusion: These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolicconversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract andmay potentiate the pharmacological effects of ginseng.

Effect of a soluble prebiotic fiber, NUTRIOSE, on the absorption of ginsenoside Rd in rats orally administered ginseng

김경아,유혜현,Wan Gu,Dae-Hyung Yu,Ming Ji Jin,Hae-Lim Choi,Kathy Yuan,Laetitia Guerin-Deremaux,김동현 고려인삼학회 2014 Journal of Ginseng Research Vol.38 No.3

Background: There is limited understanding of the effect of dietary components on the absorption ofginsenosides and their metabolites into the blood. Methods: This study investigated the pharmacokinetics of the ginseng extract and its main constituentginsenoside Rb1 in rats with or without pretreatment with a prebiotic fiber, NUTRIOSE, by liquidchromatography tandem mass spectrometry. When ginsenoside Rb1 was incubated with rat feces, itsmain metabolite was ginsenoside Rd. Results: When the intestinal microbiota of rat feces were cultured in vitro, their ginsenoside Rd-formingactivities were significantly induced by NUTRIOSE. When ginsenoside Rb1 was orally administered torats, the maximum plasma concentration (Cmax) and area under the plasma drug concentrationetimecurve (AUC) for the main metabolite, ginsenoside Rd, were 72.4 31.6 ng/mL and 663.9 285.3 mg$h/mL, respectively. When the ginseng extract (2,000 mg/kg) was orally administered, Cmax and AUC forginsenoside Rd were 906.5 330.2 ng/mL and 11,377.3 4,470.2 mg$h/mL, respectively. When ginsengextract was orally administered to rats fed NUTRIOSE containing diets (2.5%, 5%, or 10%), Cmax and AUCwere increased in the NUTRIOSE receiving groups in a dose-dependent manner. Conclusion: These findings reveal that intestinal microflora promote metabolic conversion of ginsenosideRb1 and ginseng extract to ginsenoside Rd and promote its absorption into the blood in rats. Its conversionmay be induced by prebiotic diets such as NUTRIOSE.

A prebiotic fiber increases the formation and subsequent absorption of compound K following oral administration of ginseng in rats

Kim, Kyung-Ah,Yoo, Hye Hyun,Gu, Wan,Yu, Dae-Hyung,Jin, Ming Ji,Choi, Hae-Lim,Yuan, Kathy,Guerin-Deremaux, Laetitia,Kim, Dong-Hyun The Korean Society of Ginseng 2015 Journal of Ginseng Research Vol.39 No.2

Background: Gut microflora play a crucial role in the biotransformation of ginsenosides to compound K (CK), which may affect the pharmacological effects of ginseng. Prebiotics, such as NUTRIOSE, could enhance the formation and consequent absorption of CK through the modulation of gut microbial metabolic activities. In this study, the effect of a prebiotic fiber (NUTRIOSE) on the pharmacokinetics of ginsenoside CK, a bioactive metabolite of ginsenosides, and its mechanism of action were investigated. Methods: Male Sprague-Dawley rats were given control or NUTRIOSE-containing diets (control diet + NUTRIOSE) for 2 wk, and ginseng extract or vehicle was then orally administered. Blood samples were collected to investigate the pharmacokinetics of CK using liquid chromatography-tandem mass spectrometry. Fecal activities that metabolize ginsenoside Rb1 to CK were assayed with fecal specimens or bacteria cultures. Results: When ginseng extract was orally administered to rats fed with 2.5%, 5%, or 10% NUTRIOSE containing diets, the maximum plasma concentration ($C_{max}$) and area under the plasma concentration-time curve values of CK significantly increased in a NUTRIOSE content-dependent manner. NUTRIOSE intake increased glycosidase activity and CK formation in rat intestinal contents. The CK-forming activities of intestinal microbiota cultured in vitro were significantly induced by NUTRIOSE. Conclusion: These results show that prebiotic diets, such as NUTRIOSE, may promote the metabolic conversion of ginsenosides to CK and the subsequent absorption of CK in the gastrointestinal tract and may potentiate the pharmacological effects of ginseng.