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배영민,Bae Young-Min,Daniels Lacy Korean Society of Life Science 2005 생명과학회지 Vol.15 No.2
We used the IS1096 transposon to construct Mycobacterium bovis BCG mutants resistant to an antituberculosis drug PA-824 and isolated several different mutants. We identified the locations of the insertions and found that the insertions were at various sites including the genes for the PPE proteins. HPLC analyses of the extracts of these five PPE mutant cells showed that three mutants produced only F0, and intermediate for the synthetic pathway of coenzyme $F^{420}$, and the remaining two neither F0 nor $F^{420}$. These data suggest that the products of these PPE genes are somehow involved in the biosynthesis of the coenzyme $F^{420}$.
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농축산 폐기물 처리를 위한 저온내성 (低溫耐性) 메탄 생성균의 특성에 관한 연구 - Ⅱ. 저온내성 (低溫耐性) Clostridia 의 분리 -
정광용,김재정 ( Kwang Yong Jung,Jai Joung Kim,Lacy Daniels ) 한국환경농학회 1994 한국환경농학회지 Vol.13 No.3
This study was conducted to investigate the biochemical properties of isolated bacteria, low temperature tolerant methane-producing clostridia which were selected for using them as inoculum to anaerobic fermentation of agricultural and livestock wastes at low temperature. The results were; 1. Low temperature tolerant methane-producing clostridia were isolated from the samples which showed the high methanogenesis rate by enrichment culture at low temperature in cellulose medium. These clostridia, Clostridium botulinum SRC-64, Clostridium scatologens SRC-91 and Clostridium tyrobutyricum SRC-100, were isolated from swampy sediment at latitude 56.9。N, lake sediment IV at latitude 55.0。N, and tidal land soil II at latitude 37.0。N, respectively. The optimum growth temperature for these isolates was 37℃ and the minimum, around 10℃. They all had detectable amount of F_(420), specific coenzyme of methanogens. 2. As anaerobic fermentation products of glucose SRC-64 produced H₂, acetic, isovaleric and caproic acid, SRC-91 produced H₂, propionic, butyric, valeric, and caproic acid, and SRC-100 produced only acetic and propionic acid. The isolates were produced CH₂ ranged from 2.6 to 8.68 n moles/㎖ for 2 days at 13℃.
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농축산 폐기물 처리를 위한 저온내성 (低溫耐性) 메탄 생성균의 특성에 관한 연구 - Ⅲ. 저온내성 (低溫耐性) Methanogens 의 분리 - (
정광용,김재정 ( Kwang Yong Jung,Jai Joung Kim,Lacy Daniels ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.3
This study was conducted to investigate the biochemical properties of isolated bacteria, low temperature tolerant methanogens which were selected for use as inoculum for anaerobic fermentation of agricultural and livestock wasted at low temperature. The results, obtained were summarized as follows: Low temperature tolerant methanogens were isolated from the samples which showed the high methanogenesis rate by enrichment culture at low temperature in methanol medium. These methanogens, Methanobacterium M-251 and Methanobacterium M-253 were isolated from swampy sediment at latitude 56.9°, Methanosarcina mazei M-372 from lake sediment IV at latitude 55.0。N, and Methanobacterium formicicum M-375 from tidal land soil at latitude 37.0。N, respectively. The isolated anaerobic bacteria could not use sugars as carbon sources. The optimum pH value for the growth of M-251 and M-375 was 6.8, but those for M-253 and M-372 6.5 and 7.0, respectively. The minimum growth temperature of isolated, M-251 and M-253 were 8℃ and the optimum temperature 30℃, while the minimum of M-392 and M-395 were 13℃ and the optimum 37℃. The growth rate of isolates at 17.5℃ were lower by 32-50% than that of 30℃. The isolated Methanobacterium strains such as M-251, M-253, and M-375 have lower cell yield, 0.38-1.21g/1M CH₄than 1.14-1.51g/1M CH₄of Methanosarcina mazei M-372.
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