Selenium 공급방법이 수경재배 토마토의 생장과 Se 흡수에 미치는 영향

이철규(Cheol-Kyu Lee),조경철(Kyung-Cheol Cho),이정현(Jeong-Hyun Lee),조자용(Ja-Yong Cho),서범석(Beom-Seok Seo),양원모(Won-Mo Yang) (사)한국생물환경조절학회 2005 생물환경조절학회지 Vol.14 No.4

토마토 ‘모모타로’를 공시하여 셀레늄 10㎎ㆍL?¹의 관주 및 엽면시비 등의 단용 및 혼용처리가 수경재배 토마토의 생육 및 과실 내 셀레늄 축적 함량에 미치는 영향을 구명하였다. 토마토 종자를 50공 트레이에 파종하여 70일 동안 육묘한 후 유묘를 코코피트 슬라이브를 이용한 수경재배 시스템에 정식하였다. 양액은 일본원예시험장 배양액 표준처방으로 조성하였으며, pH 5.8~6.2와 EC 2.3 ㎎ㆍL?¹ 등으로 조절하여 공급하였다. 셀레늄은 무기태 SeO₂와 sugar fatty acid ester에 킬레이트화 한 유기태 셀레늄을 10 ㎎ㆍL?¹으로 조성하여 관주, 엽면시비, 관주와 엽면시비를 병행하여 처리하였다. 초장, 엽수, 엽면적 및 엽록소 등의 토마토 생장반응은 셀레늄의 엽면시비, 그리고 엽면시비와 관주를 병행한 처리구에서 현저히 증가하였다. 과실 내 셀레늄 축적 함량은 킬레이트화 한 유기태 셀레늄을 엽면시비와 관주를 병행하여 처리한 경우에 0.302 ㎎ㆍL?¹으로 가장 높았다. 무기태와 유기태 셀레늄의 엽면시비와 관주 등의 단용 처리 보다는 혼용 처리가 전반적인 과실 생장과 체내 셀레늄 축적에 효과적이었다. 무기태 설레늄(SeO₂) 보다는 sugar fatty acid ester에 킬레이트화한 유기태 셀레늄 처리가 셀레늄을 함유한 기능성 토마토의 수경재배에 더 효과적이었다. This study was conducted to clarify the effects of supplying methods of selenium on the growth and Se uptake of hydroponically grown tomato plants. Tomato seeds (Lycopersicum esculentum Mill. cv. Momotaro T-93, Daki Seed Co.) were sown in plug tray with fifty holes, and raised for sixty days. Tomato seedlings transplanted to coco fiber slabs were supplied with the nutrient solutions adjusted to EC 2.3 dSㆍm?¹ and pH 5.8~6.2 recommended by the Japanese Horticultural Experiment Station. Selenium forms used were inorganic SeO₂ (here in after referred to Se) and organic selenium chelated with sugar fatty acid ester (here in after referred to chelated-Se). 10 ppm selenium solutions were treated to tomato plants with foliar applications, drenching, and foliar application plus drenching. Growth characteristics in terms of plant height, number of leaves, leaf area and chlorophyll content were significantly increased in the plot of foliar application of Se, and in the plot of foliar application plus drenching of chelated-Se than other plots, respectively. Transported contents of selenium into the tomato fruits were highest as 0.302 ppm in the plot of foliar application plus drenching of chelated-Se. Also, it had tended to be higher in the plot of foliar application plus drenching than in the plots of foliar application or drenching in both of Se and chelated-Se. Foliar application and drenching of organic chelated-Se were effective to produce the functional tomato fruits.

어린이급식관리지원센터의 사업규모에 따른 직원의 직무중요도, 직무수행도, 직무만족도 및 이직의도 분석

서원경(Won Kyung Seo),노정옥(Jeong Ok Rho) 한국식품영양과학회 2020 한국식품영양과학회지 Vol.49 No.9

본 연구는 전국의 어린이급식관리지원센터 중 총 30개 센터, 237명의 직원을 대상으로 2018년 11월부터 12월까지 센터 사업규모를 4억 이하와 5억 이상으로 구분하여 직무중요도, 직무수행도, 직무만족도 및 이직의도를 조사하였다. 직무중요도는 사업규모 5억 이상(4.39)이 4억 이하(4.31) 센터보다 높으며, 직무수행도는 4억 이하(4.23)가 5억 이상(4.19) 센터보다 높은 경향이었다. 중요도와 수행도가 가장 높은 직무는 순회방문 업무이며, 특화사업의 중요도와 수행도가 가장 낮았다. 순회방문 업무는 센터의 설립근거가 되는 업무로 IPA 분석의 4분면 중 A 영역에 해당하였다. 사업규모의 차이 없이 ‘예산관리’의 중요도는 높았으며 식품영양전공자로서 수행의 어려움이 있는 직무임에도 불구하고 높은 수행도를 보였다. 직무만족도는 5억 이상(3.73)이 4억 이하 (3.49) 센터보다 유의적으로 높았으며, 사업규모의 구분 없이 ‘동료와의 관계’의 만족도가 가장 높으며, ‘급여 및 복리후생’이 가장 낮은 수준이었다. 직원들은 본인들이 수행하는 노력에 비해 받는 급여가 공정하지 않다고 생각하며 복리후생제도에 대한 만족도가 매우 낮았는데, 이는 직원들의 이직의도를 높이는 주요 요인으로 판단된다. 특히, 지금보다 나은 직장으로의 이직의도는 4억 이하(3.41)가 5억 이상(3.20) 센터보다 유의적으로 높았다. 직무중요도와 직무수행도 간의 상관관계는 모든 사업규모에서 높은 양의 상관관계를 보였다. 그러나 직무중요도, 직무수행도와 직무만족도 간의 상관관계는 5억 이상 센터에서만 양의 상관관계를 보였다. 이는 5억 이상 센터의 직무만족도가 4억 이하 센터보다 높기 때문이라 생각한다. 직무만족도는 이직의도와 음의 상관관계를 보였는데, 특히 4억 이하 센터 직원의 이직의도는 사업규모가 큰 센터의 직원보다 높았다. 그러나 센터 직원의 잦은 이직은 원장과 교육대상자들의 불만족을 초래할 수 있으며, 교육의 연속성과 질을 낮게 하는 원인이 되므로 이에 대한 대책이 필요하겠다. 이상의 결과, 급식센터 직원의 직무만족도와 이에 따른 이직의도는 사업규모에 따른 편차를 보였다. 따라서 직원의 근로계약 방법 개선을 통해 안정감이 필요하며, 승진시스템 도입을 통해 직무에 대한 책임감을 높일 필요가 있겠다. 특히, 소규모 센터의 경우 직원교육의 업무별 전문화가 필요하며, 규모가 큰 센터와의 협업을 통한 업무 효율화가 필요하겠다. 직원의 높은 직무만족도는 수행도를 높임으로써 센터의 고객인 어린이, 원장, 조리 종사자 및 학부모 등의 만족도에 긍정적으로 영향을 미치므로 식품의약품안전처와 중앙어린이급식관리지원센터는 지역센터 직원의 근무환경에 대한 보다 세심한 관심과 지원이 요구되겠다. This study examined the status of job importance, job performance, job satisfaction, and turnover intention of employees at the Center for Children’s Foodservice Management (CCFSM) according to budget size as well as investigated correlations among these factors. The participants were employees of CCFSM with budgets under 400 million won (n=80) and CCFSM over 500 million won (n=157). The demographic characteristics and status of job importance, job performance, job satisfaction, and turnover intention were assessed using a self-administered questionnaire. The average scores of job importance and performance in CCFSM with budgets under 400 million won were 4.31 and 4.23 points, whereas scores for CCFSM with budgets over 500 million won were 4.39 points and 4.19 points, respectively. Average scores of job satisfaction at CCFSM with budgets over 500 million won were significantly higher than scores at CCFSM with budgets under 400 million won (P<0.01). Average scores of turnover intention at CCFSM with budgets under 400 million won were significantly higher compared to CCFSM with budgets over 500 million won (P<0.05). For CCFSM with budgets under 400 million won, a positive correlation between job importance and job performance was observed (P<0.001). For CCFSM with budgets over 500 million won, positive correlations were observed between job importance and job performance (P<0.001) and job satisfaction (P<0.01) as well as a positive correlation between job performance and job satisfaction (P<0.01). Regardless of budget size, a negative correlation between job satisfaction and turnover intention was detected (P<0.01, P<0.001). Therefore, in order to reduce turnover intention, satisfaction factors with low scores and the working environment should be improved.

Protective Effecf of Brazilin on Cisplatin Nephrotoxicity

Seo, Kyung Won,Kong, Jae Myeong,Choung, Se Young 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.2

Cisplatin is one of the most effective antitumor agents currently available for cancer therapy. However, its clinical use has been limited by its severe side effects, especially nephrotoxicity. Therefore, brazilin, which has a radical scavenging effect, was given intraperitoneally to evaluate the effect on cisplatin nephrotoxicity in rats. Remarkable protective effects against nephrotoxicity of cisplatin were observed when brazilin was administered to rats simultaneously with cisplatin. Hepatotoxicity induced by combination treatment of cisplatin and brazilin was evaluated by measuring serum glutamic pyruvate transaminase and serum glutamic oxalate transaminase. Combination treatment did not affect the levels of sGPT and sGOT, and any combination treatment did not induce metallothionein in kidney. Brazilin which has radical scavenging effect directly reduced nephrotoxicity of cisplatin in vivo. Thus, it seems that nephrotoxicity of cisplatin was caused by free radicals. The present results indicate that brazilin, when it is given with cisplatin, may provide protection against cisplatin nephrotoxicity in rats.

Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve

Seo, NaRi,Lee, Sung-Ho,Ju, Kyung Won,Woo, JaeMan,Kim, BongJu,Kim, SoungMin,Jahng, Jeong Won,Lee, Jong-Ho Medknow PublicationsMedia Pvt Ltd 2018 Neural regeneration research Vol.13 No.1

<P>Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field (PEMF) reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using <I>in vitro</I> and <I>in vivo</I> experiments. In <I>in vitro</I> experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 (Schwann cell marker), glial fibrillary acidic protein (astrocyte marker), and brain-derived neurotrophic factor and nerve growth factor (neurotrophic factors) mRNA expression. In the <I>in vivo</I> experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site (1 × 10<SUP>6</SUP> cells). DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. <I>In vitro</I> experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. <I>In vivo</I> experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.</P>

Clinical implications in laboratory parameter values in acute Kawasaki disease for early diagnosis and proper treatment

Seo, Yu-Mi,Kang, Hyun-Mi,Lee, Sung-Churl,Yu, Jae-Won,Kil, Hong-Ryang,Rhim, Jung-Woo,Han, Ji-Whan,Lee, Kyung-Yil The Korean Pediatric Society 2018 Clinical and Experimental Pediatrics (CEP) Vol.61 No.5

Purpose: This study aimed to analyse laboratory values according to fever duration, and evaluate the relationship across these values during the acute phase of Kawasaki disease (KD) to aid in the early diagnosis for early-presenting KD and incomplete KD patients. Methods: Clinical and laboratory data of patients with KD (n=615) were evaluated according to duration of fever at presentation, and were compared between patients with and without coronary artery lesions (CALs). For evaluation of the relationships across laboratory indices, patients with a fever duration of 5 days or 6 days were used (n=204). Results: The mean fever duration was $6.6{\pm}2.3days$, and the proportions of patients with CALs was 19.3% (n=114). C-reactive proteins (CRPs) and neutrophil differential values were highest and hemoglobin, albumin, and lymphocyte differential values were lowest in the 6-day group. Patients with CALs had longer total fever duration, higher CRP and neutrophil differential values and lower hemoglobin and albumin values compared to patients without CALs. CRP, albumin, neutrophil differential, and hemoglobin values at the peak inflammation stage of KD showed positive or negative correlations each other. Conclusion: The severity of systemic inflammation in KD was reflected in the laboratory values including CRP, neutrophil differential, albumin, and hemoglobin. Observing changes in these laboratory parameters by repeated examinations prior to the peak of inflammation in acute KD may aid in diagnosis of early-presenting KD patients.

Subacute oral toxicity and bacterial mutagenicity study of Korean Red Ginseng oil

Seo, Hwi Won,Suh, Jae Hyun,So, Seung-Ho,Kyung, Jong-Soo,Kim, Yong-Soon,Han, Chang-Kyun The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.4

Background: Red ginseng oil (RGO) is produced by supercritical $CO_2$ extraction of secondary products derived from Korean Red Ginseng extract. As the use of RGO has increased, product safety concerns have become more important. Methods: In the present study, the subacute oral toxicity and bacterial reverse mutagenicity of RGO were evaluated. Sprague-Dawley rats were orally administered with RGO for 28 d by gavage. Daily RGO dose concentrations were 0 mg/kg body weight (bw), 500 mg/kg bw, 1,000 mg/kg bw, or 2,000 mg/kg bw per day. Bacterial reverse mutation tests included five bacterial strains (Escherichia coli WP2 and Salmonella typhimurium TA98, TA100, TA1535, and TA1537), which were used in the presence or absence of metabolic activation. The plated incorporation method for mutation test was used with RGO concentrations ranging from $312.5{\mu}g$ to $5,000{\mu}g$ per plate. Results: The subacute oral toxicity test results did not reveal any marked changes in clinical characteristics. There were no toxicological changes related to RGO administration in hematological and serum biochemical characteristics in either control or treatment animals. Furthermore, no gross or histopathological changes related to RGO treatment were observed. The bacterial reverse mutation test results did not reveal, at any RGO concentration level and in all bacterial strains, any increase in the number of revertant colonies in the RGO treatment group compared to that in the negative control group. Conclusion: The no-observed-adverse-effect level of RGO is greater than 2,000 mg/kg bw and RGO did not induce genotoxicity related to bacterial reverse mutations.

Exercise training causes a partial improvement through increasing testosterone and eNOS for erectile function in middle-aged rats

Seo, Dae Yun,Lee, Sung Ryul,Kwak, Hyo Bum,Park, Hyuntea,Seo, Kyo Won,Noh, Yeon Hee,Song, Kang-Moon,Ryu, Ji-Kan,Ko, Kyung Soo,Rhee, Byoung Doo,Han, Jin Elsevier 2018 Experimental Gerontology Vol.108 No.-

<P><B>Abstract</B></P> <P><B>Purpose</B></P> <P>Aging changes the balance of sex hormones and causes endothelial dysfunction in the penis, both of which are important determinants of erectile dysfunction (ED). The purpose of this study was to evaluate whether exercise training could protect against erectile dysfunction by increasing serum testosterone and penile eNOS levels in aging rats.</P> <P><B>Methods</B></P> <P>A total of 14 young (2-month-old) and 14 middle-aged (18-month-old) Sprague Dawley rats were randomly assigned to either untrained control (young control, [YC], middle-aged control, [MC]) or endurance exercise-trained (young exercise, [YE], middle-aged exercise, [ME]) groups with seven rats per group. The exercise groups trained with treadmill running for 6 weeks. Body composition parameters (body weight, heart mass, liver mass, and testicular mass), serum sex hormone levels (testosterone, luteinizing hormone, follicle-stimulating hormone, and prolactin), endothelial function-related parameters in the penis (endothelial nitric oxide synthase [eNOS], CD31, alpha smooth muscle actin [α-SMA]), and maximal intracavernous pressure measure (ICP) and total ICP were analyzed in middle-aged rats.</P> <P><B>Results</B></P> <P>The middle-aged groups showed increased body weight, as compared with the young groups, but exercise training attenuated the aging-induced increase in body weight. The middle-aged groups had lower testicular mass compared with the young groups, but exercise training attenuated aging-induced decreases in testicular mass. Exercise training increased serum testosterone levels in both the young and middle-aged groups. However, there were no changes in the levels of luteinizing hormone, follicle-stimulating hormone, and prolactin among the groups. MC group showed decreased protein levels of p-eNOS, as compared with the YC group. However, exercise training protected against aging-induced decrease in eNOS and p-eNOS protein levels in the penis. Interestingly, exercise training also increased protein levels of α-SMA and maximal ICP in the middle-aged group.</P> <P><B>Conclusions</B></P> <P>Exercise training has beneficial effects on erectile function in aged rats through increased testosterone production from the testis and strengthening of the cavernous endothelium with activation of eNOS. Therefore, exercise training may be a therapeutic modality for improving erectile dysfunction associated with aging.</P>

How to judiciously manage iron deficiency anemia after bariatric surgery?

Kyung Won Seo(서경원) 대한외과학회 2014 대한외과학회 학술대회 초록집 Vol.2014 No.11

Impact of seasonal vertical stratification on the dispersion patterns of dredging plumes off the south coast of Korea

Seo, Jun Young,Ha, Ho Kyung,Im, Jungho,Hwang, Jin Hwan,Choi, Sun Min,Won, Nam-Il,Kim, Youngsung Elsevier 2018 Marine geology Vol.395 No.-

<P><B>Abstract</B></P> <P>Simultaneous satellite and ship-borne surveys have been conducted to investigate the dispersion patterns of dredging plumes off the south coast of Korea in two contrasting seasons (spring and autumn). The distribution of total suspended solid (TSS) derived from sequential satellite imagery showed that the dredging plumes in spring developed over longer distances along the surface current than those in autumn. In spring, the deep and strong stratified condition in the water column contributed to the increase in radius of plume dispersion along the water surface. In autumn, the shallow and vertically well-mixed (or un-stratified) condition in the water column enhanced sinking of suspended dredged materials. This difference is due to the Brunt-Väisälä frequency being higher in spring (0.0183s<SUP>−1</SUP>) than in autumn (0.0123s<SUP>−1</SUP>). Although the sediment-laden overflow significantly increased TSS near the surface during the dredging period, the TSS contribution to water column stratification was only 2.3−5.6% of the thermohaline contribution. Therefore, the seasonal variation of thermohaline density is the main factor determining the dispersion patterns of dredging plumes in the study area.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dredging plumes in spring developed over longer distances along the surface current than those in autumn. </LI> <LI> Suspended sediment contribution from dredging to water column stability is only 2.3–5.6% of thermohaline contribution. </LI> <LI> Seasonal variation of thermohaline density is the main factor determining the dispersion patterns of dredging plumes. </LI> </UL> </P>

Enhanced electrical properties of PEDOT:PSS films using solvent treatment and its application to ITO-free organic light-emitting diodes

Seo, Yoon Kyung,Joo, Chul Woong,Lee, Jonghee,Han, Joo Won,Lee, Dong Jin,Entifar, Siti Aisyah Nurmaulia,Kim, Soukyoon,Cho, Nam Sung,Kim, Yong Hyun Elsevier 2017 Journal of luminescence Vol.187 No.-

<P><B>Abstract</B></P> <P>Highly conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) films are prepared by introducing a new solvent 2-ethoxyethanol and are optimized by acid-free solvent post treatment. The behavior of samples are investigated with various coating conditions. The change of electrical performance for 2-ethoxyethanol added PEDOT:PSS films with various post treatment methods is studied. Upon post treatment, the sheet resistance greatly decreases attributed to a structural change with removal of insulating PSS in the film. Based on these conductive films, we demonstrate efficient ITO-free green phosphorescent organic light-emitting diodes (OLEDs). The efficiency of OLEDs with post-treated PEDOT:PSS electrodes is greater than that of OLEDs with untreated PEDOT:PSS electrodes. The results illustrate a promising future for flexible, low-cost, ITO-free OLEDs employing PEDOT:PSS electrodes optimized by 2-ethoxyethaol with acid-free solvent post treatment.</P>