생약제제인 이공산(異功散)의 Cisplatin 유도 신장독성 보호 및 항산화 효과

Lee, Kyung-Tae,Ahn, Kyoo-Seok,Chang, Sung-Goo,Oh, Soo-Myung,Jung, Jee-Chang 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung-Tae Lee, Kyoo-Seok Ahn¹, Sung-Goo Chang², Soo-Myung Oh²and Jee-Chang Jung²¹College of Pharmacy, ²Oriental Medicine and ³College of Medicine, and East-West Medical Reserch Institute, Kyung Hee University, Seoul, Korea. Preventive and Antioxidative Effects of Crude Drug Preparation(E-kong-san) on Cisplatin Induced Nephrotoxicity. Proceedings of International Symposium on East-West Medicine, Seoul. 201-211, 1999. -Nephroprotective effects of a crude drug-prearation (E-kong-san) were determined from cisplatin on rabbit kidney proximal tubule and human renal cortical cells by MTT assays and sustained glucose consumption on ciplatin-induced human renal cortical tissue. Levels of creatinine and blood ure nitrogen(BUN) in serum after administration of cisplatin(0.75mg/kg,i.p.) to E-kong-san(0.75g/kg/day,p.o.)-pretreated rats were markedly lower compared to those of cisplatin-treated rats. Moreover, the administration of E-kong-san significantly inhibited the loss of body weight of cisplatin injected rts. These findings suggest that E-kong-san on the cisplatin induced nephrotoxicity led us to investigate whether the effect of this water extract was a result of anti-oxidation. E-kong-san showed strong free radical scavenger activities on 1,1-dipheny1-2picrylhydrazil(DPPH) radical and xanthine/xanthine oxidase(XOD) generated superoxide anion radical(O2-). We further studied the effects of E-kong-san on lipid peroxidation in rat liver microsomes induced by enzymatic and nonenzymatic methods. E-kong-san exhibited significant inhibition on both ascorbic acid/Fe2+and ADP/NADPH/Fe3+ induced lipid peroxidation in rat liver microsomes. Based on these results, we suggest that-E-kong-san attenuate the cisplatin induced cytotoxicity and its mechanism can be eplained by antioxidant.

Association of 20 potential ATP2B1‐interacting genes with blood pressure in Koreans

Kyung‐Won Hong,Hyun‐Seok Jin,Ji‐Eun Lim,Bermseok Oh 한국유전학회 2011 Genes & Genomics Vol.33 No.3

Plasma membrane calcium‐transporting ATPase 1 (ATP2B1)is associated significantly with blood pressure in Caucasians and Asians. ATP2B1 regulates calcium homeostasis and belongs to the P‐type calcium pump family; several studies have identified diverse proteins that bind to ATP2B1. We hypothesized that ATP2B1 regulates blood pressure through ATP2B1‐interacting genes. To this end, 20 potential ATP2B1‐interacting genes were selected, 197 SNPs of which were analyzed for their association of systolic and diastolic blood pressure. These 20 genes were categorized into 2 groups: ATP2B1‐binding genes and ATP2B1‐cleaving calpain family members. Three ATP2B1‐binding genes (CALM1, NOS1, and PDLIM1)were associated with blood pressure, and a SNP in CALM1(rs2401887) generated the strongest association signal (beta=‐3.60±0.92, p=8.9×10^(‐5) for systolic blood pressure and beta=‐1.40±0.62, p=0.02 for diastolic blood pressure). Of the calpain family members, 3 genes (CAPN6, CAPN9, and CAPN10)were associated with blood pressure, and the CAPN10 SNP rs4676348 yielded the strongest association signal (beta=‐0.88±0.27, p=0.001 for systolic blood pressure and beta=‐0.58±0.18, p=0.015 for diastolic blood pressure). Further, the interaction of CALM1 to ATP2B1 was examined using the blood pressure of individuals who carried both variants of CALM1and ATP2B1 genes. Similarly the interaction of CAPN10 to ATP2B1 was also examined. The CALM1 variant (rs2401887)and CAPN10 variants (rs4676348) appear to decrease blood pressure further in addition to the decrease by the variant (rs17249754) of ATP2B1, which suggests that ATP2B1 might regulate blood pressure through the ATP2B1‐interacting genes CALM1 and CAPN10.

부인과질환, 월경통과 여성 체형 및 체온의 상관성 연구

최석영 ( Seok Young Choi ),안지윤 ( Ji Yoon Ahn ),이미주 ( Mi Joo Lee ),이진무 ( Jin Moo Lee ),장준복 ( Jun Bock Jang ),이경섭 ( Kyung Sub Lee ),이창훈 ( Chang Hoon Lee ) 대한한방부인과학회 2012 大韓韓方婦人科學會誌 Vol.25 No.4

Objectives: A women`s body type or body temperature is often thought to be associated with gynecologic disorders or dysmenorrhea. The purpose of this study is to analyse the characteristics of body type and body temperature in relation to gynecologic disorders and dysmenorrhea. Methods: We studied 40 patients visiting Kangnam Kyung-hee Medical Center from Jan. 1st, 2010 to March 31st, 2012. Body type and body temperature were compared between Gynecologic disorder group and non-Gynecologic disorder group. Body type and body temperature were compared again between Dysmenorrhea group and non-Dysmenorrhea group. SPSS 18.0 for Windows was used to analyse the data. Results: There was no statistically significance difference in body type and body temperature between Gynecologic disorder group and non-Gynecologic disorder group. Dysmenorrhea group had more developed lower body and greater temperature difference between lower abdomen and foot compared to non-Dysmenorrhea group. Conclusions: The results suggest that women with developed lower body and greater temperature difference between abdomen and lower extremity are more prone to dysmenorrhea.

척추질환 입원환자의 연도별 변동추이에 대한 통계적 고찰

박경수 ( Kyung Su Park ),이준환 ( Jun Hwan Lee ),정석희 ( Seok Hee Chung ) 한방재활의학과학회 2006 한방재활의학과학회지 Vol.16 No.4

Objectives : The aim of this study was to find the change in the number of inpatient with spine disease, data were reviewed on inpatients who was diagnosed as spine disease in the Kyung-hee Medical Center(KHMC) for 25 years(1980~2004). Methods : Analysis data of the number of spinal disease was based on the KHMC annual report for 25 years(1980~2004). Results : 1. The number of inpatient in KHMC with spine disease has been increasing during 25years from 1980 to 2004(p-value:0.000) 2. The number of inpatient in KHMC with ankylosing spodylitis(AS) is increasing during 25years from 1980 to 2004(p-value=0.000) 3. The number of inpatient in KHMC with spinal stenosis is increasing during 25years from 1980 to 2004(p-value=0.000) 4. The number of inpatient in KHMC with herniated nucleus pulposus of cervical spine(HNP of C-spine) is increasing during 25years from 1980 to 2004(p-value=0.000) 5. The number of inpatient in KHMC with herniated nucleus pulposus of Lumbar spine(HNP of L-spine) is increasing during 25years from 1980 to 2004 (p-value=0.007) Conclusions : It is suggesting that the number of patients of spinal stenosis and HNP of L-spine increase at KHMC.

Jo,Kyung-Yul

조경열,최윤수,석경순 대구효성가톨릭대학교 1986 연구논문집 Vol.32 No.1

A new spectropotometric determination of citric acid was examined using carbonyldiimidazole (CDI) which has been used in petide synthesis. CDI was reacted with citric acid to form yellow acyl-imidazole derivative (λ??443nm) in dichloroethane. This colored reaction was so characteristic with tricarboxylic acid that can be used selective determination of citric acid in common carboxylic acid. As increasing the reaction temperature, the acyl compound was decomposed to produce the substance of which physical property was similar to the hydrolized compound. Citric acied was calibrated in the range of 1-6μg/ml and the extinction coefficient of the acyl-imidazole compound was 28000 in this condition.

S-470 Relationships between blood pressure and clinico-pathological findings in IgA nephropathy

( Hyung-seok Ihm ),( Da-rae Kim ),( Jin-sug Kim ),( Kyung-hwan Jeoung ),( Tae-won Lee ),( Chun-gyoo Ihm ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

Objective: Several factors contribute to the development of hypertension in patients with IgA nephropathy (IgAN). This study was conducted to find the relationships between baseline blood pressure (BP) and clinico-pathological findings in patients with IgAN and normal renal function. Design and Method: Clinico-pathological findings were analyzed in a total of 163 patients with IgAN and serum creatinine ￡ 1.1 mg/dL from The Kyung-Hee Cohort of Glomerulonephritis. The glomerular surface area (GSA) was determined using imaging analysis software. The serum and urine angiotensinogen (AGT) concentrations were measured using human ELISA kits.?Results: Mean serum creatinine concentration was 0.86 (0.5~1.1) mg/dL. Systolic BP was ≥ 130 mmHg in 72 patients (44%) and ≥ 140 mmHg in 42 (26%). Patients with systolic BP ≥ 130 mmHg as compared with those <130 mmHg had higher GSA and tubulointerstitial fibrosis and showed worse follow-up clinical findings: higher concentrations of serum creatinine, larger amount of proteinuria and lower levels of GFR. Systolic BP was positively correlated with age, baseline and follow-up proteinuria, serum uric acid concentrations and IgM deposit and negatively with follow-up GFR and the slope of change in 1/serum creatinine for 2 years, while it has no significant relationships with serum and urine AGT and 24 hour urinary sodium excretion. Conclusions: This study showed that systolic BP was associated with renal progression and pathological findings, glomerulomegaly and tubulointerstitial fibrosis, in patients with IgAN.

자연휴양림 이용자 요구사항 및 만족

서경석 ( Kyung Seok Seo ),윤성훈 ( Sung Hun Yoon ),김갑열 ( Gab Youl Kim ) 한국감정평가학회 2011 감정평가학논집 Vol.10 No.2

The research was studied about measure how each element affect to recreational forest visitors. And I can provide the useful information for the strategic decision-making to make value-up through characterization and differentiation of recreational forest. Those who use recreational forest were studied. Survey area was divided into five regions; Kyung-gi province, Kang-won province, Chung-chong province, Kyung-sang province and Jeon-ra province. And then national and local governments, individuals were classified as operating principals. Empirical analysis were analyzed by applying the Kano model. The questionnaire was analyzed by statistical methods; for data processing, along with a test of reliability in the measuring tools, frequency analysis. Human services and nature management components of recreational forest were classified as one-dimensional quality. Most users are more concerned about these elements. Recreational forest visitors are more concerned about quality associated with software components rather than hardware components. Recreational forest visitors with high interests and a high level of satisfaction about recreational forest has more concerned about the quality.

사상체질과 신체형태지수와의 연관성

박경아,김희진,허경석,안수경,이성호,이종은,이원택 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

자궁경부암에서 혈관 내피 성장인자 ( VEGF ) 및 VEGF Mrna 의 발현에 대한 연구

이선경(Seon Kyung Lee),김승보(Seung Bo Kim),지성길(Sung Gil Chi),염윤석(Yoon Seok Yum),이주희(Ju Hee Lee) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.1

N/A Objective : Angiogenesis, the formation of blood vessels by sprouting from pre-existing ones, is essential for the growth of solid tumors beyond 2~3mm in diameter and for tumor metastasis. Vascular endothelial growth factor (VEGF), is known as vascular permeability factor(VPF) and mediates vascularization and tumor-induced angiogenesis. This study examined the potential of growth, invasion, and metastasis of uterine cervical carcinomas associated with neovascularization. Methods : From January 1996 to December 1999, at the Department of Obstetrics and Gynecology, Kyung-Hee University Hospital, 37 uterine cervical carcinomas and 7 normal cervical tissues were obtained and the samples were immediately frozen and stored at -70℃. Immunohistochemical staining for VEGF was carried out to study VEGF localization, and the levels of VEGF subtype mRNAs were determined by quantitative RT-PCR in specimens. The relation between VEGF subtypes expression of cervical cancers was analysed. Results : The positive staining for VEGF is seen dominantly in the cytoplasm of the cancer cells, and faintly in interstitial cells. The intensity of staining was stronger in squamous carcinomas than in adenocrcinomas, but there was no significant difference (p>0.05). Quantitative RT-PCR analysis demonstrated significantly increased VEGF121/VEGF165 mRNA expression levels (>0.56/>0.72) in 21 (56.8%) and 15 (40.5%) of 37 cervical carcinomas comparing to control groups (mean: 0.28/0.36). There was no obvious relationship between VEGF121/VEGF165 mRNA expression levels and the clinical parameters examined including age, pathology, differentiation, tumor size, lymphovascular space invasion, LN involvement and invasion depth except clinical stage (p<0.05). Conclusions : The overexpression of VEGF mRNA may be an important contributing factor in cervical carcinomas. There is no significant differenece of VEGF mRNAs levels according to clinical parameters, so it seems that the expression of VEGF is involved in the promotion of angiogenesis on cervical cancer and plays an important role in early invasion.

간세포암과 감별이 어려웠던 악성중피종 치험 1예

김선문,허원석,채경훈,강윤세,정재훈,김연수,박기오,문희석,이엄석,김석현,성재규,이병석,이헌영,신경숙,조준식,송인상,강대영 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Malignant peritoneal mesothelioma is a rare neoplasm that arises from the mesothelium of a serosal cavity and is a rapidly fatal disease with a median survival of 4 to 12 months for untreated cases. Recently, we experienced a case with malignant peritoneal mesothelioma who was suspected hepatocelluar carcioma by abdominal CT scan and was confirmed by biopsy including immunohistochemical stain(calretinin) after surgery. We performed tumor excisions and wedge resection of the liver(segment Ⅷ)and inserted Tencoff catheter in abdominal cavity at 25th day of post-operation. We treated with intraperitoneal paclitaxel(25mg/m^(2)/day for 5 days) six courses monthly. She was well tolerable and is still living without any evidence of recurrence for 14th month of post-operation.