갯쑥부쟁이(Heteropappus hispidus (Thunb.))의 감마방사선 감수성과 M1 세대 형질변동 특징

오병권,홍경애,송성준,유장걸,이선주,이영일 제주대학교 방사능이용연구소 2003 연구보고 Vol.17 No.-

제주도에서 자생하는 갯쑥부정이(Heteropappus hispidus(Thunb.))에 감마선을 10, 20, 40, 80, 120, 160, 200, 300, 400Gy 수준으로 나누어서 조사한 뒤 발아율 및 발아 30일 후의 본엽 출현을, LD_(50), 본엽길이, 줄기부분의 안토시아닌 색소 형성 유무, 생육 60일 후의 로젯형 엽, multi-shoot 형성율, 초장, 채화 결실율등의 주요 양적형질들을 생존개체를 대상으로 실시하였다. 120Gy 까지의 발아율은 88.5%로 대조구와 큰 차이를 보이지 않았으나 160Gy 이상에서 부터는 급격히 감소하여 치사선량(LD_(50))은 160Gy 범위 정도였다. 또한, 본엽 출연율과 그 길이는 조사선량이 높아질수록 억제되었다. 안토시아닌 색소 형성은 조사전량이 높을수록 대조구 2%에서 30%로 높아졌으며 로젯형도 고선량 처리구에서 발생하였다. Multi-shooting은 80Gy 이상일 때 부터 나타났으며 multi-shooting을 형성하는 개체들은 초장은 짧고 줄기는 가늘게 다발형으로 자라는 특성이 있어서 만일 형질이 고정된다면 화분 관상용 개체로 선발하기에 적합하였다. 특히, 조사선량이 증가됨에 따라서 안토시아닌 색소 형성, 로젯형 개체, multi-shoot등의 개체가 많이 출현되었는데 이는 돌연변이 유기의 선발지표가 될 수 있을 것으로 보인다. The seeds of naturally growing Heteropappus hispidus Thunb were treated by nine different doses(0, 10, 20, 40, 80, 120, 160, 200, 300, 400㏉) of gamma rays to investigate their germination rate and to quantify the characteristics of their germinated plant as like as leaf appearance and length, the formation rate of anthocyanin color in stem 30 days after germination, the formation rate of rosette leaf and multi-shoot, the flowering and seed-bearing, and shoot length. The germination rate at least up to 120 ㏉ was not greatly affected but was rapidly decreased at over 160 ㏉. It seemed that lethal dose(LD_(50)) of germination was 160 ㏉. The leaf appearance and growth was also inhibited, but the formation rate of anthocyanin color in the flower stem was enhanced up to 30% with dose. The rosette plants were observed in plants irradiated with higher than 40 ㏉. Multi-shoots were developed over 80 ㏉. For a short shoot length and bundle of thin stem, it was considered that they can be selected as the potential pot flower plants, through genetic fixation. In particular, it was suggested that the formation of anthocyanin color in flower stem, rosette and multi-shoot plants induced by the morphologically markers for the mutant selection of Heteropappus hispidus (Thunb.)

초등학교 교사의 영양교육에 대한 인식 조사 연구

김경애,정난희,오순희 한국가정과학회 2004 한국가정과학회지 Vol.7 No.2

The purpose of this study was to investigate the status of teacher's perception about the nutrition education of elementary school. The results of this study were as follows. Most of the teachers responded that nutrition education necessitate in curriculum of elementary school, the main reason for necessity of nutrition education was for proper growth, they thought starting period of nutrition education was to begin when children are in a kindergarten and a infant home. The perception for a suitable person to teach the nutrition education showed dietician, parents, class teacher and a related teacher in oder and the time of that is a related class, as a spare time at everyday, a special activity time, and service a meal in order. Importances of the contents of a nutrition education were proper eating habits, growth and nutrition, a food hygiene and a disease in order. Problems of elementary students' nutrition were a unbalanced eating, a ingestion a processing and instant food too much, lack of table manner, fatness and a weak child in order. The most of expected effects through the nutrition education were a good table habits and manners, the way of a efficient nutrition education were a need of link with parents, a need of link the dietician, a teacher's class and training in order. The average score of nutrition knowledge was 6.3?6.9 out of 10 and teachers of Gwangju scored more higher on nutrition knowledge than teachers of Jeonnam, as they arranged a dietician was so.

청각장애 아동의 언어학습 특성을 반영한 웹 기반 언어학습 시스템의 구현

금경애,권오준,김태석 한국컴퓨터교육학회 2004 컴퓨터교육학회 논문지 Vol.7 No.4

청각장애 아동은, 듣고 발화하는 과정을 통해 언어를 재구성해가는 건청 아동과는 달리, 청력의 손실로 인해 언어 습득의 선천적 메커니즘이 작용될 수 없다. 따라서 청각장애 아동의 언어능력 향상을 위해 웹 기반 언어학습 시스템은 이들의 언어학습 특성에 적합하게 구성되어야 한다. 청각장애아동을 위한 웹기반 언어학습 시스템을 설계할 때, 동작이나 상황을 나타내는 말을 동적으로 구현하고, 사물 및 행동에 대한 관찰력을 증진시킬 수 있도록 주도적인 상황언어학습의 시스템 구성이 필요하다. 그리고 대체사고전략의 활용과 얼굴표정의 강조, 반대어와 대비어의 활용이 시스템 구성에 적용되어야 한다. 이 연구에서는 청각장애 아동의 문법적 오류의 감소와 언어학습의 향상을 도모할 수 있도록 이들의 특성에 적합한 언어학습 시스템을 제안한다. For children with hearing impairment, unlike the children without hearing impairment who can reconstruct their languages through the process of hearing and uttering, the inherent mechanism for language acquisition do not operate due to the loss of hearing ability. Therefore, to help hearing-impaired children develop their language ability, web-based language learning system should be constructed depending on the special qualities which the children possess in language learning process. When the system is being designed, it is necessary that words or expressions describing actions or situations be animated and that active situation-based language learning system be constructed to help them develop their power of observation. Moreover, the system needs to be developed through the use of alternative thinking strategy, antonyms, and contrastive words, and emphasis on facial expressions. This paper presents web-based language learning system which is suitable for hearing-impaired children in the way to reduce the grammatical errors they make and to improve their language learning.

급성 췌장염의 합병이 추정되는 한국형 출혈열 1예

박영수,김창오,김영근,홍성관,장경희,허애정,염준섭,송영구,김준명 대한감염학회 2001 감염 Vol.33 No.5

The Korean hemorrhagic fever (KHF) is an acute febrile disease with characteristic of fever, bleeding tendency, and renal failure. There are many complications of Korean hemorrhagic fever such as infection, anemia, internal bleeding, hypopituitarism, respiratory, and neurologic complication. A few cases were reported on acute pancreatitis with hemorrhagic fever abroad, but there was no case about Korean hemorrhagic fever with acute pancreatitis in this country. We experienced a case of Korean hemorrhagic fever associated with suspected acute pancreatits. With review of articles, we report a case of 51 year-old woman with KHF, where acute pancreatitis developed during management, (Korean J Infect Dis 33:376∼379, 2001)

의약분업 전후의 의약정보서비스의 질의응답과 질의자의 만족도 분석

신정인,김미애,허경희,김미정,신현택,오정미 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Drug Information Centers(DICs) are responsible for providing updated and relevant drug information on the efficacy, safety and quality of drugs to health-care practitioners and finally to patients. After the establishment of new prescription law(Bunup)' on August 2000, the future direction for DICs is strongly needed to provide specified, appropriate and rapid information to health-card practitioners and patients. This project was undertaken to provide a future direction of DICs via comparing differences of Q&A before and after Bunup, based on the analysis of Q&A worksheets that were conducted from August 1999 to July 2001 at Drug Information Research Institute(DIRI) of Sookmyung Women's University. In addition, feedback sheets were collected from the users of DIRI from April 2001 to August 2001, to evaluate the satisfaction scores by '5-point Likert scale' on the response. The number of inquiries responded by DIRI was increase from 201 to 574 and the most frequently asked inquirers were pharmacists, specifically community pharmacists. The mean time to respond before and after Bunup was 5.35 and 4.68 hours, respectively. The method of inquiry utilized the most was electronic mailing system followed by telephone both before and after Bunup(66.2% vs. 65.7% via e-mail, from 32.8% vs. 32.4% via telephone). The most frequently asked category of question was on the 'clinical drug' category both before and after Bunup (79% vs. 73.6%). The tertiary literature was the most frequently used reference to answer the inquiries. Mean feedback result via questionnaires was good. That included accuracy, quality of information, time to respond, attitude of provider, accessibility, etc. The most increased inquirer after Bunup was general person, compared with before. Therefore DICs should provide separate responds according to inquirers, develop the systemic program to improve the response for each request, have systemic Q&A worksheet, and develop the educational program for drug information providers. DICs should continuously provide more appropriate and rapid information to health-care practitioners and patients.

하루 콩단백질 25g 섭취를 위한 메뉴작성 및 영양성분 분석

한재숙,김정애,서봉순,이연정,서향순,조연숙,한경필,이신정,오옥희,우경자,조은자,구성자,김수진,李承彦,南出隆久 동아시아식생활학회 2002 동아시아식생활학회지 Vol.12 No.2

The purpose of this study was to develop menus for daily intake of 25g soybean protein and to analyse nutrients of these foods. Analytical values were compared to the theoretical one using the food composition table and recommended dietary allowances for Koreans (7th revision). The results are as follows. 1. Soybean curd residue stew, Soybean curd, Kimchi saute, and hard boiled soybean and lotus root were selected for the menu for January, of which the content of soy bean protein(SBP) was 33.1g, soybean stew, soybean curd and soybean sprout saute, and Italian deep fried soybean curd were for February, of which the content of SBP was 35.0g. The content of SBP in soybean paste soup with soybean curd, fried soybean curd and fried soybean curd roll, the menu for March, was 24.9g. That of April were soybean curd gratin with soymilk, soybean curd and ham with garlic dressing and the content of SBP was 26.3g. That of May were soybean porridge, soybean flour cake with honey (Dasik), soybean sprout soup and the content of SBP was 26.7g. That of June were soymilk, pan-fried soybean curd, steamed soybean curd with chicken and the content of SBP was 28.4g. That of July were noodle with soymilk, mapatofu, soybean curd salad and the content of SBP was 24.7g. That of August were soybean sprout with mustard dressing, Tossed green pepper with raw soybean flour, Tofu and Kimchi stew, soybean curd steak and the content of SBP was 26.2g. That of September were Chinese cabbage soup with raw soybean flour, sweet and sour tofu and the content of SBP was 23.2g. That of Oct. were Fermented soybean stew, soybean pan cake and the content of SBP was 24.3g. That of November were not-pressed soybean curd casserole, pan-fried mashed soybean curd with egg, stir frying deep-fried soybean curd with vegetables and the content of SBP was 22.4g. That of December were soybean curd and mushroom casserole, fried soybean curd and vegetables, hard boiled soybean curd and the content of SBP was 28.9g. 2. The ratio of the analytical value over theoretical value (A/B%) of one serving in kcal, carbohydrate, protein, fat, ash and dietary fiber were 57.7~107.7%, 42.9~131.9%, 79.2~118.3%, 54.5~100%, 40.7~80.8% and 42.1~113.2%, respectively. 3. The ratio of A/B% of one serving in Ca, K, Na, P and Fe were 44.1~93.6%, 59.0~153.1%, 53.1~117.7% 64.6%~138.8 and 33.8~77.3%, respectively. That in Fe was the lowest among minerals. 4. The ratio of analytical value over Korean R.D.A(A/C%) in Ca was relatively higher (22.0~85.9%) than that in kcal(18~63%). 5. The ratio of A/C% in Fe was 25.1~64.3% and lower than that in Ca and protein in general.

배추 시스테인 단백질 분해 효소 억제 유전자 BCPI-1의 발현과 종자 발아 및 유묘 생장과의 연관성

홍준기,이은영,김정률,양경애,최영주,정우식,김호일,윤대진,이상열,조무제,임체오 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Phytocystatins are protein inhibitors of cysteine proteinases of the papain family that have been identified in both monocot and dicot plants. A cDNA encoding a phytocystatin, BCPI-1 (Brassica Cysteine Proteinase Inhibitor-1) has been isolated from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds. Here, we tested whether BCPI-1 transcription is regulated by hormones, and could be involved in regulating cysteine proteinases during and after germination. BCPI-1 was sensitive to exogenous GA_(3) and ABA, which are important factors controlling seed germination, suggesting that the expression of BCPI-1 is hormonally regulated. We introduced a recombinant plasmid containing the full-length BCPI-1 cDNA under the control of cauliflower mosaic virus 35S promoter into rice embryogenic calli using the particle delivery method, and regenerated a number of transgenic rice plants. Constitutively over-expressed BCPI-1 caused changes in overall plant growth and development, including reduced germination and seedling growth. These data support the role of the BCPI-1 in the regulation of endogenous proteinases during both seed germination and subsequent seedling development. Phylocystatins은 papain계열의 cysteine 단백질 분해 효소 활성을 특이적으로 억제하는 억제자로 다양한 식물 종으로부터 분리되었다. 본고에서는, 배추 화아 cDNA library로부터 분리된 phytocystatin인 BCPI-1(Brassica Cysteine Proteinase Inhibitor-1)의 생체 내 기능에 대해 연구하였다. 먼저, BCPI-1 전사체는 발아 조절에 중요한 영향을 미치는 GA₃와 ABA에 의해 예민하게 증가, 혹은 감소되는 반응을 보임으로써, BCPI-1이 식물 호르몬의 영향을 받으며, 특히 발아나 유묘의 생장 조절에 관련이 있음을 알 수 있었다. Particle bombardment 방법을 통하여 BCPI-1을 벼의 배 형성 세포 내에 도입, 재분화 시켜 형질 전환 벼를 생성하였다. CaMV 35S promoter의 조절에 의해 지속적인 BCPI-1 발현을 보이는 형질 전환 벼의 경우, 발아와 유묘 생장이 현저히 지연되었다. 위의 결과들을 바탕으로, BCPI-1이 식물체 내에서 생성되는 cysteine 계열의 단밸질 분해 효소 활성을 조절하여 종자의 발아와 유묘의 생장에 영향을 미치는 것으로 추측한다.

Self-Representation and Korean Honorific Shifts

( Kyung Ae Oh ) 한국언어정보학회 2014 언어와 정보 Vol.18 No.1

This study discovers the dynamic nature of an interactional hierarchy as well as an institutional hierarchy in the use of Korean honorifics. Data was collected from the conversations of two Korean female interlocutors. The interlocutors met for the first time in the U.S. and often changed their use of honorifics. The paper examines the method in which the two interlocutors negotiate hierarchies during interaction and how the negotiation is reflected in their use of honorific shifts. The paper also investigates honorific shifts in terms of self-representation to suggest that there is another hierarchy at work other than the institutional hierarchy. An examination of the data shows that the shifts occurred not randomly but strategically. The findings suggest that 1) interlocutors may negotiate interactional hierarchy during their conversation, often in the same sentence, 2) interactional hierarchy often cross the boundary of the institutional hierarchy to obtain interactional goals, in this case, intimacy, and 3) the utterance contents may play a significant role in the interlocutors`` honorific shifts.

The Effectiveness of Code-switching and Identity Reconstruction

Kyung-Ae Oh,Young Mi Kim 한국영어어문교육학회 2012 영어어문교육 Vol.18 No.4

The study aimed to determine the outcomes of one teacher’s code-switching between Korean and English in a Teaching English in English (TEE) university class. Seventy-four female freshmen participated in class observations, a survey questionnaire, and interviews. The results were categorized into three parts: 1) instances of code-switching, 2) students’ perceptions, and 3) the relationship between code-switching and students’ identity reconstruction. The seven categories of code-switching were: 1) to build rapport between the teacher and students, 2) to assist group discussions, 3) as a repair strategy, 4) to teach bilingual terminology, 5) to disapprove students’ behavior, 6) to announce important notices, and 7) to clarify the meaning of vocabulary. Students had positive perceptions concerning code-switching, in particular, content knowledge delivery and classroom management. More importantly, the teacher’s code-switching influenced the students’ self-identity. This finding implies that code-switching is an effective educational strategy that teachers need to apply based not only on the type of course and the students’ English proficiency levels, but also on the students’ cultural and social context where they construct their identity.

Korean Economics Students’ Perceptions Concerning EMI

Kyung-Ae Oh 한국응용언어학회 2011 응용 언어학 Vol.27 No.3

This study investigates students’ perceptions concerning English as a Medium of Instruction (EMI) course. Quite a few studies have been conducted regarding EMI, but few in the field of economics. This study explores students’ motivation, satisfaction, problems with English language skills, and coping strategies regarding EMI by conducting research in an EMI course offered by a department of economics. Based on the results, this study suggests the most appropriate language prerequisite course for EMI. Fifty students participated in the survey questionnaires, and the results from the survey analysis were complemented by the findings from interview analysis. The results reveal that 1) students feel motivated to take an EMI course, 2) students are satisfied with content knowledge acquisition and English language learning through EMI, 3) instructors and students use code-switching to increase lecture comprehensibility, and 4) among three types of CBI courses, the adjunct course might be best suitable for an English language prerequisite course.