導入種마늘의 主要形質과 生育特性에 關한 硏究 : Allium sativum L.

李敬姬,黃海鎭,吳仲烈,韓相政 대구효성가톨릭대학교 응용과학연구소 1995 응용과학연구논문집 Vol.4 No.-

導入育種의 一環으로 中國産 마늘 가운데서 球의 形質로 보아 優秀하다고 認定되는 上海種과 瀋陽種을 우리나라 代表品種인 義城種(寒地型) 및 濟州種(暖地型)과 함께 溫室과 圃場栽培 實驗으로 이들의 性能을 檢討한 결과를 要約하면 다음과 같다. 1) 上海, 瀋陽 및 濟州種은 無低溫區와 2∼3℃의 20日, 40日 및 60日間 低溫處理區에서 모두 播種後 約 1週日이 經過한 10月 20日 頃부터 萌芽하기 始作하였다. 萌芽期間은 上海種이 19∼22日, 그리고 濟州種이 17∼26日로서 低溫處理期間이 延長됨에 따라 短縮되었으나 瀋陽種은 低溫處理 與否에 關係없이 9∼12日 所要되었다. 2) 寒地型인 義城種은 無低溫區에서 播種後 74日이 經過한 12月 27日에 萌芽하기 始作하였으며, 低溫處理期間이 延長됨에 따라 漸次 빨라져서 60日間 處理區에서는 播種後 13日이 經過한 10月 26日에 萌芽가 始作되었다. 그리고 萌芽期間이 延長됨에 따라 顯著히 短縮되었다. 따라서 上海種과 瀋陽種은 暖地型이라고 認定되었다. 3) 圃場栽培에서 瀋陽種이 다른 品種에 比하여 根重, 球重 및 球當鱗片種이 가장 무거웠고, 球當鱗片數가 많았으며 또한 球高, 球徑 및 莖徑이 커서 品種間 差異가 있었으나 莖徑/球徑比는 供試 4品種 모두 0.2였다. 4) 上海種은 草長, 莖長 및 花梗長이 매우 짧으며 珠芽重은 0.3g로서 가장 가볍고 葉數가 많으며 葉幅이 컸다. 또한 暖地型인 上海, 瀋陽 및 濟州種의 추대기는 5月 12日로서 추대율은 100%였으나 寒地型인 義城種의 추대기는 6月 8日로서 추대율은 30%였다. 5) 供試 4品種의 植物全體의 乾物重은 播種後 135日 經過한 4月 7日부터, 그리고 球의 乾物重은 播種後 149日이 經過한 4月 21日부터 急激히 增加하였는데 이들中 瀋陽種이 가장 旺盛한 增加를 보였고 그 다음이 上海種이였다. 6) 供試 4品種의 줄기의 乾物重은 播種後 135日이 經過한 4월 7日부터 急激히 增加하였는데 이들중 上海種이 가장 旺盛하게 增加하였고 그 다음이 瀋陽種이였다. 그리고 잎의 乾物重은 緩慢하게 增加하였으나 瀋陽種이 가장 무거웠다. 葉面積은 瀋陽種이 가장 넓으며, 早期에 增加하기 始作하고 그 速度도 빨랐다. 7) 暖地型인 瀋陽種은 上海와 濟州種보다 早生으로서 生育이 旺盛하여 球重이 무겁고 燁數가 많으며 葉面積이 넓어서 우리나라 濟州道와 南海岸地帶에서 普通栽培와 冬季施設栽培에 利用할 수 있으리라고 믿어졌다. As a part of introduction breeding, two Chinese garlic cultivars, Shanghai and Shenyang, were cultivated in greenhouse and in field with two typical Korean cultivars, Euisung and Cheju, southern and northern ecotype respectively, and the characters and growth characteristics were investigated. 1. Shanghai, Shenyang and Cheju cultivars treated with low temperature of 2 ~ 3℃ for 20, 40 and 60 days and also the nontreated ones began to sprout from Oct. 20, about one week after planting. Sprouting periods were 19 ~ 22 and 17 ~ 26 days in Shanghai and Cheju cultivar, respectively. These periods were shortened as the low temperature treating periods were extended, but Shanghai cultivar needed 9 ~ 12 days for sprouting regardless of low temperature treatment. 2. A northern ecotype, Euisung cultivar, began to sprout on Dec. 27, 74 days after planting in period was extended, for 20 ~ 66 days, and began to sprout Oct.26, 13 days after planting. From this result, Shanghai and Shenyang cultivars were recognized as a southern ecotypes. 3. In field cultivation, root weight, bulb weight and clove weight per bulb were superior in Shenyang among all the four cultivars and had many cloves per bulb and also showed superior bulb height and blub diameter. These characters represented differences among cultivars, but the ratio of stem diameter to bulb diameter was 0.2 in all four cultivars. 4. Heights of plant, stem and flower stalk were the longest and the bulblet weight, 7.0g, were the heaviest in Shanghai cultivar. In Shenyang cultivar, the flower stalk was very short and the bulblet weight was light, 0.3g, but had many leaves and showed wide leaf width Shanghai, Shenyang and Cheju cultivars, southern ecotype ones, bolted on May 12 and the bolting ration was 100%, but Euisung cultivar, nothern ecotype, bolted on Jun. 8 and the bolting ra-tio was 30%. 5. Dry weight of whole plant and the bulb increased markedly from Apr. 7, 135 days after planting, and from Apr. 21, 149 days after planting, respectively, in all four cultivars, and Shenyang showed the most vigorous increment and Shanghai was next. 6. Stem dry weight increased markedly from Apr. 7, 135 days after planting in all four cultivars; and Shanghai showed the most vigorous increment and Shanghai was next. Leaf dry weight increased gradually and that of Shenyang were the heaviest one. Leaf area of Shenyang cultivar was most broad and grew early and rapidly. 7. Shenyang cultivar, a southern ecotype, grew earlier as compared with Shanghai and Cheju cultivars, and grew vigorously, and the bulb weight was heavy and had many broad leaves. It was considered that these two Chinese cultivars are possible to cultivate in Cheju and south coast districts of the Korean Peninsula by common method and in greenhouse during winter season.

조직배양에서 얻어진 쪽파의 유전적 변이체들이 보여주는 단백질과 Isozyme의 유형분석

朴敬淑,李在悅,徐奉甫 慶北大學校 1992 論文集 Vol.52 No.-

Some genetic variants of Amphihaploid, Hypoamphidiploid, Amphidiploid are regenerated from tissue culture in Allium wakegi. They were applied to investigate the effects of the chromosomal differences on the gene expression of some isozymes. The electrophoretic migration patterns of total proteins were different at the position of MW5×10^5 Dalton among the genetic variants. Three different isozymes were characterized from the migration patterns of isozymes in the discontinuous polyacrylamide gel electrophoresis. Alcohol dehydrogenase(ADH) appeared 3 bands in the gel electrophoresis corresponding to 3 isozymes, that is allozymes distributed as single isozyme in each individual. Isocitric acid dehydrogenase(ICDH) appeared 5 bands in the gel and corresponds to 5 isozymes as allozymes which distributed single isozyme in each individual. Phosphoglucoisomerase(PGI) appeared 8 bands in electrophoresis and to 8 isozymes which originated from 2 genes and distributed more than two isozumes in each individual. According to the isozume expression in the genetic variants of Allium wagegi, the amplification of chromosomal numbers can affect neither to increase the isozyme numbers and concentration nor to express heterogeneous types in the genetic variants.

생쥐 2-세포기 수정란의 초급속동결

강만종,이철상,한용만,유대열,이경광 제주대학교 농과대학 제주도축산문제연구소 1991 畜産論叢 Vol.6 No.1

This study was carried out in order to investigate eggects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos Mouse 2-cell embryos. fol-lowing dehydration by exposure to DMSO and sucrose. were directly immersed into liquid nitrogen and thawed in 37℃ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium a t various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO conentrations (82.6%). However, When sucrose concentrations of 0.25 and 0.5M were added to the freezing medium with 3.0M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time(2.5min) in 3.0M DMSO +0.25M sucrose(85.9%). 3. The development rate of embryos at in vitro 2-celL in vivo 2-celL solution control and untreated control was 84.6%, 90.9%, 89.9%. and 89.7%. respectively.

건축설계 프로세스별 협력설계 의사결정 지원모델 구성 방안

오승준,신창현,이경국,전재열 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1

In Korea construction project case, architectural construction project is curtailed production because information network process within multidisciplinary isn't smooth. Particularly, the construction is not insufficient about performance, cost and material, construction process, etc. in result various question arises because of design error in construction step. And various mistake is made because communication path within multidisciplinary isn't smooth in architectural design and design change process. Therefore The final object of this study is to propose the establishment method of decision support model for the cooperative design in order to provides improved design coordination and optimize the building system.

중증 대뇌반구경색 환자의 사망률과 초기 예후인자

나해리,강우열,정승철,유경호,마효일,이병철 대한노인병학회 2001 대한노인병학회지 Vol.5 No.2

Occurrence of Apple scar viroid-Korean strain (ASSVd-K) in Apples Cultivated in Korea

Lee, Ju-Hee,Park, Jean-Kyung,Lee, Dong-Hyuk,Uhm, Jae-Youl,Ghim, Sa-Youl,Lee, Jai-Youl The Korean Society of Plant Pathology 2001 Plant Pathology Journal Vol.17 No.5

Apple is the most economically important fruit in Korea. The suspected viroid disease of dapple apple was found in apple fruits cultivated in Kyungpook province. Symptoms begin in mid-July as small circular spots, which stand out against the background color on the young fruit. Dappling of the fruit becomes more intense and easier to detect as the fruit approaches maturity; the affected spots remain yellowish as the fruit matures. no leaf or bark syndromes have been associated with this disease. The infected fruits are downgraded considerably during quality grading. The low molecular weight RNA containing viroid RNA molecules were extracted from the peels of the apples with dapple symptoms. The RNA molecules were extracted from the apples using Qiagen column chromatography. The purified RNAs were used for the synthesis of cDNA with RT-PCR. The PCR products were then ligated into a pGEM-T Easy vector, cloned and sequenced. The sequence of the viroid RNA molecule shows 331 nucleotides with one base difference ("G" insertion between the position of 133 and 134) compared with that of the Apple scar skin viroid (ASSVd) reported by Hashimoto and Koganezawa in Japan. This is the first report on the occurrence of the ASSVd in apple trees cultivated in Korea, as well as the identification of a new Korean strain of the ASSVd.the ASSVd.

Effects of caloric restriction on the expression of lipocalin-2 and its receptor in the brown adipose tissue of high-fat diet-fed mice

Kyung-Ah Park,Zhen Jin,Hyeong Seok An,Jong Youl Lee,Eun Ae Jeong,Eun Bee Choi,Kyung Eun Kim,Hyun Joo Shin,Jung Eun Lee,Gu Seob Roh 대한약리학회 2019 The Korean Journal of Physiology & Pharmacology Vol.23 No.5

Obesity causes inflammation and impairs thermogenic functions in brown adipose tissue (BAT). The adipokine lipocalin 2 (LCN2) has been implicated in inflammation and obesity. Herein, we investigated the protective effects of caloric restriction (CR) on LCN2-mediated inflammation and oxidative stress in the BAT of high-fat diet (HFD)-fed mice. Mice were fed a HFD for 20 weeks and then either continued on the HFD or subjected to CR for the next 12 weeks. CR led to the browning of the white fat-like phenotype in HFD-fed mice. Increased expressions of LCN2 and its receptor in the BAT of HFD-fed mice were significantly attenuated by CR. Additionally, HFD+CR-fed mice had fewer neutrophils and macrophages expressing LCN2 and iron-positive cells than HFD-fed mice. Further, oxidative stress and mitochondrial fission induced by a HFD were also significantly attenuated by CR. Our findings indicate that the protective effects of CR on inflammation and oxidative stress in the BAT of obese mice may be associated with regulation of LCN2.

Zingerone protects keratinocyte stem cells from UVB-induced damage

Lee, Jienny,Oh, Sae Woong,Shin, Seoung Woo,Lee, Kyung-Woo,Cho, Jae-Youl,Lee, Jongsung Elsevier 2018 Chemico-biological interactions Vol.279 No.-

<P><B>Abstract</B></P> <P>The epidermis, the outermost layer of the skin, is a stratified epithelium that protects the body from the external environment. Keratinocyte stem cells (KSCs) are involved in epidermis homeostasis by maintaining epidermal integrity through a process of constant regeneration. Ultraviolet B (UVB) radiation is a major inducer of cellular damage in the epidermis. In this study, we investigated the effects of zingerone (a phenolic compound derived from spices) on UVB-induced cellular damage in KSCs. We found that zingerone significantly inhibited cellular senescence of KSCs in response to UVB irradiation. These effects were confirmed by the senescence-associated β-galactosidase and comet assays. Zingerone decreased the production of proinflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in UVB-irradiated KSCs. Moreover, UVB-induced expression of p21, a cell cycle arrest-related gene, was reduced by zingerone treatment, whereas zingerone upregulated the expression of proliferation-related genes such as proliferating cell nuclear antigen (PCNA) and vascular endothelial growth factor (VEGF), in addition to anti-senescence-related genes including telomerase reverse transcriptase (TERT), histone deacetylase 1 (HDAC1), and DNA (cytosine-5)-methyltransferase 1 (DNMT1). The UVB-protective effects of zingerone were mediated by inhibition of p42/44 MAPK and p38 MAPK. Therefore, zingerone could potentially be used to protect the epidermis from UVB-induced damage.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Zingerone inhibited the UVB-induced cellular senescence in KSCs. </LI> <LI> Zingerone reduced the UVB-induced production of proinflammatory cytokines. </LI> <LI> Zingerone reduced the UVB-induced expression of p21 gene. </LI> <LI> Zingerone increased expression of genes such as PCNA, VEGF, TERT, HDAC1, and DNMT1. </LI> <LI> Zingerone inhibited UVB-induced activation of p42/44 MAPK and p38 MAPK. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A study on the patterns of expression of the DAZ and HSP genes in the testicular tissue of men with azoospermia

Lee, Ho-Joon,Lee, Hyoung-Song,Song, Gyun-Jee,Byun, Hye-Kyung,Cho, Youl-Hee,Kim, Jong-Hyun,Seo, Ju-Tae,Lee, Yoo-Sik Korean Society of Medical Genetics 1997 대한의학유전학회지 Vol.1 No.1

Spermatogenesis is known to be regulated by a number of genes and several factors such as hormones, growth factors, cytokines and others. This study was done to evaluate the relationship between HSPs and DAZ genes in human spermatogenesis; we observed the expression pattern of HSP gene in azoospermia men with DAZ gene that regulated the gene expression related with human spermatogenesis. RT-PCR method was used to detect DAZ, HSP70A, and HSP70B transcripts in all RNA samples. Total RNA was extracted from 21 testis tissues using TRIZOL reagent. cDNAs were synthesized with reverse transcriptase, AMV. All PCR reaction were performed on a PCR themocycler with DAZ, HSP70A, and HSP70B-specific primers. Semen analysis, karyotyping and testis histology were performed. DAZ gene, known as a candidate gene of azoospermia factor(AZF), was deleted in 2 of 21 patients. To evaluate the only effects of HSPs in this patients, 2 DAZ deleted cases were removed. We observed the mRNA of HSP70B in 5 whereas none could be seen with regard to HSP70A. Furthermore, the sperm of these 5 men were discovered to be immature. In conclusion, HSP70B as well ad DAZ gene seem to be involved causing spermatogenic failure. We suggest that HSP70B plays an important role in spermatogenesis and it is one of factors induced sperm maturation in human.

Ubiquitylation and degradation of adenomatous polyposis coli by MKRN1 enhances Wnt/β-catenin signaling

Lee, Hae-Kyung,Lee, Eun-Woo,Seo, Jinho,Jeong, Manhyung,Lee, Seon-Hyeong,Kim, Soo-Youl,Jho, Eek-Hoon,Choi, Chel Hun,Chung, Joon-Yong,Song, Jaewhan Springer Science and Business Media LLC 2018 Oncogene Vol.37 No.31