Differential Manifestation of Ocular Phenotypes in TALEN-mediated p19arf Knockout FVB/N and C57BL/6J mouse lines

Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9

Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

관상동맥 스텐트 삽입술을 시행한 환자에서 안지오텐신 전환효소 억제제와 안지오텐신 수용체 차단제가 재협착에 미치는 영향

김연경,정진원,윤경호,박은미,송미진,최준호,이은미,유남진,김남호,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

손상받은고양이 줄무늬체에서 축삭반응에 관한 미세구조적 연구

양남길,조영열,안의태,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

SVD 알고리즘 및 HMM을 이용한 얼굴 및 눈 패턴 검출

진경찬,김명남,신장규,손병기,조진호 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

The studies about automatic pattern detection of the eye and face from the human image acquired by the CCD image sensor have good applicabilities in the industry, home automation, and data communication field. In general, pattern detection method consists of feature based matching and template matching. In feature based matching, the feature vector is extracted with DLM(dynamic linking matching), EBGM(elastic bunch graph matching), HMM(hidden markov model) matching and knowledge based matching using statistical characteristics. In template matching, in general, the template vector is extracted with PCA(principal component analysis). When these method applied in the face and eye detection, each method has its own merits and some disadvantage. Therefore, by combined utilization of SVD(singular value decomposition) and HMM algorithm, is expected that we can selectively make use of each methods advantage and it result in improved detection accuracy. In this paper, we proposed the method for face and eye detection, which was combined by the two algorithms, to be suitable for the high speed image processing using DSP chip or microprocessor. In the beginning, template matching was followed by a template extraction using batch SVD algorithm and then face pattern was classified and recognized by HMM algorithm which is one of feature based matching technique. Finally, eye pattern detection was performed by pattern search neural network utilizing eigeneye image.

대학무용수의 사회적 지지와 스트레스 대처 방식에 관한 연구

남지경,박진희 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this study was to diagnose the relation among the type of dance major, social backup and stress coping behavior. To achieve the purpose, 232 dance majors from 5 universities in Seoul and metropolitan area were random-sampled. To rate social backup, Interpersonal Relations Perception Questionnaire used by Cohen and Hoberman in 1983 was used. To rate coping behavior on stress, The Ways of Coping Checkiist developed by Folkman and Lazarus in 1985 and translated and standardized by Kim, Jung-hee and Lee, Jang-ho in 1985 was used. For data process, One-way Analysis of Variance and Multiple Regression Model was used. Through these research tool and process, the result as follows were achieved. First, there were partial difference in social backup and stress coping behavior according to demographic variabies. Referring to social backup, the group with higher standard of knowledge generally showed more positive perception of social backup and the group with higher income discerned higher feelings of belonging. Referring to the religion, feeling of belonging to Christianity or Buddhism were higher than that of Catholic or pagan On the matter of stress coping behaviour, the higher grade the group was, the more stress coping behavior were shown and, in many cases, it was problem oriented coping behavior. This tendency is similar according to the amount of income. According to the type of religion, in many cases, probiem-oriented coping behavior and social backup seeking behavior were shown. Second, the more positively the dancer recognize of the dance major activity and the more highly recognize social backup, the more various types of stress coping behavior occurred except for coping behavior with wishful thinking.

태산반석산이 면역기능에 미치는 영향

남경수,김희진,이태균 동국대학교 생명과학연구소 1995 생명과학연구논문집 Vol.2 No.-

To invesitigate the immunoadjuvant effect of Taesanbanseok-san(TBS), TBS was pretreated to Ballb/c mice for 10 days with intraperitioneally. After 10 days, haptenic antigen (VCR-BSA, vincristine-bovine serum albumin) complexed with Freund's complete adjuvant was immunized. The results are as follows. The antibody production in Balb/c mouse immunized with VCR-BSA was markably increased in the pretreated group of TBS compared with saline treated(control) group. The number of macrophage in Balb/c mouse immunized with VCR-BSA was considerably increased about 2.3 times in the pretreated group of TBS compared with control group. The lysozyme activity of macrophage in Balb/c mouse that was immunized with VCR-BSA was increased gradually in the pretreated groups of TBS compared with control group. In the TBS pretreated group, B cell proliferation was increased with a dose dependent manner. At the concentration of twenty microliter/well of TBS, B cell proliferation activity was most highest. On the other hand, in the T proliferation, TBS pretreated group had less considerable activity than B cell proliferation. These results may suggest that TBS will have immunoadjuvant effects on the cells which concerned with immuno mechanism.

제산제 탄산수산화마그네슘의 최적 제조조건 추구 및 분체학적 성질

남진경,이계주 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

Magnesium carbonate hydroxide (hydrotalcite) was synthesized to pursue the optimum conditions having the highest acid consuming capacity by a Box-Wilson experimental design, and micromeritical properties of samples were evaluated by laser particle size analyzer, SRD, BET system, mercury porosimeter, and several adsorption capacity test. The optimum preparation conditions for hydrotalcite were as followwws: concentration of aluminum hydroxide and magnesium hydroxide 1.17mole, reaction temp. 82.6℃, reaction time 2.32hr, ratio of (Al(OH)_3/Mg(OH)_2 4.25, drying temperature 93.2℃ respectively, and its acid consuming capacity was 286ml/g. the neutralization curve shows that sample whose acid consuming capacity was the highest has the best neutralizing capacity and duration capacity. The crystalline form of the synthesized hydrotalcite was identified with a hexagonal system, and the d (003) spacing value of three samples was large. a part of alumina crystal form was shown at 2θ=18°, 38° in sample 3. The micromeritical properties of the synthesized hydrotalcite has no relation with an acid consuming capacity, and there was a tendency that the higher the acid consuming capacity is the larger the particle size and the narrower the size distribution. The values of pore invasive volume, pore area and specific surface area of sample 1 were small among three samples. Isotherm by BET system forms hyteresis loop and it shows the synthesized hydrotacite was porous. the pore diameter was about 10Å and it verifies mesopore. The adsorption capacity measured by methylene blue has no relation with acid consuming capacity. The adsorption of quinine sulfate in aqueous solutuion was identical with the Langmuir isotherm.

조대술에 의한 하마종의 치료

강동균,황경문,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

하마종은 구강저에 발생하는 점액 남종으로 주로 설하선과 관련된다. 즉, 하마종은 설하선 분비도관의 파열로 인하여 타액이 분비도관에서 빠져나와 주위의 연조직 내에 저류됨으로써 발생하는 가성 낭으로 정의 되며, 원인은 설하선 도관의 파열이나 폐색이다. 하마종의 임상소견은 청색의 투명한 종창이 구강저의 소대를 중심으로 편측으로 발생하며, 무통성이며, 촉진시 파동성을 나타낸다. 조직소견은 결합 조직 내 와동이 형성되지만, 낭 벽은 상피세포가 아닌 육아조직으로 구성되는 가성 낭을 관찰할 수 있다. 하마종의 첫 번째 치료법으로는 하마종의 내벽이 구강점막의 일부가 되도록 만들어 주는 조대술을 고려할 수 있다. 본 증례는 특징적인 하마종의 임상소견을 보이는 환아에게 gauze-packing등을 동반한 조대술을 이용하여 치료한 증례로써 양호한 치료결과를 보여 보고하고자 한다. Ranula is a mucocele which occurs at the mouth floor and mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue. but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.