Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

경도 및 중등도 본태성 고혈압환자에서 Amlodipine단독요법

안기완,서영환,김상도,박윤환,김철,조경상,김완중,홍순표 최신의학사 1992 最新醫學 Vol.35 No.9

자가부식형 상아질접착제와 레진시멘트와의 적합성에 관한 연구

김도완,박상진,최경규 大韓齒科保存學會 2005 Restorative Dentistry & Endodontics Vol.30 No.6

본 연구는 상아질 접착제와 레진 시멘트의 적합성 여부를 알아보고자 시행하였다. 발거한 제3대구치의 상아질 표면을 노출시킨 후 Tescera ATL 복합레진 시편을 상아질 접착제 [All Bond 2 (Bisco), Clearfil SE Bond (Kuraray), Adper Prompt L-POP (3M), One-Up Bond F (Tokuyama)]와 레진시멘트 [Choice (Bisco), Panavia F (Kuraray), RelyX ARC (3M), Bistite II DC (Tokuyama)]로 접착하고 미세인장 결합강도 및 주사전자 현미경 관찰을 시행한 결론은 다음과 같다. 1. Clearfil SE Bond와 All-Bond 2가 Prompt L-Pop과 One-Up Bond F 보다 높은 미세인장 결합강도를 보였다(p < 0.05). 2. Clearfil SE Bond와 All-Bond2 사용시 1-step 상아질 접착제보다 혼성층이 두껍고 레진 tag가 길었다. This study was performed to investigate the compatibility between 4 dentin adhesives and 4 resin luting cements. Dentin adhesives used in this study were All-Bond 2 (Bisco Inc., Schaumbrug, IL, USA), Clearfil SE-Bond (Kuraray Medical Inc, Osaka, Japan), Prompt L-Pop (3M Dental Products, St. Paul, MN, USA), One-Up Bond F (Tokuyama corp., Tokyo, Japan). Resin luting cements used in this study were Choice (Bisco Inc., Schaumbrug, IL, USA), Panavia F (Kuraray Medical Inc, Osaka, Japan), RelyX ARC (3M Dental Products, St. Paul, MN, USA), Bistite II DC (Tokuyama corp., Tokyo, Japan). Combination of each dentin adhesive and corresponding resin cement was made to 16 experimental groups. Flat dentin surfaces was created on mid-coronal dentin of extracted mandibular third molars, then dentin surface was polished with 320-grit silicon carbide abrasive papers. Indirect resin composite block (Tescera, Bisco) was fabricated. Its surface for bonding to tooth was polished with silicon carbide abrasive papers. Each dentin adhesive was treated on tooth surface and resin composite overlay were luted with each resin cement. Each bonded specimen was poured in epoxy resin and sectioned occluso-gingivally into 1.0 ㎜ thick slab, then further sectioned into 1.0 × 1.0 ㎟ composite-dentin beams. Microtensile bond strength was tested at a crosshead speed of 1.0 ㎜/min. The data were analysed by one-way ANOVA and Duncan's multiple comparison tests. The results of this study were as follows; 2-step self-etching dentin adhesive which has additional bonding resin is more compatible than 1-step self-etching dentin adhesive.

고압 수증기 내에서 산화막 형성에 관한 연구

박경희,안순의,구경완,왕진석 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.2

This paper shows experimentally that oxide layer on the p-type Si-substrate can grow at low temperature(500℃∼600℃) using high pressure water vapor system. As the result of experiment, oxide layer growth rate is about 0.19Å/min at 500℃, 0.43Å/min at 550℃, 1.2Å/min at 600℃ respectively. So, we know oxide layer growth follows reaction-controlled mechanism in given temperature range. Consequently, granting that oxide layer growth rate increases linearly to temperature over 600℃, we can expect oxide growth rate is 5.2Å/min at 1000℃. High pressure oxidation of silicon is particularly attractive for the thick oxidation of power MOSFET, because thermal oxide layers can grow at relatively low temperature in run times comparable to typical high-temperature. 1 atm conditions. In the condition of higher-temperature and high-pressure steam oxidation. the oxidation time is reduced significantly.

피스톤 측정 시스템 개발에 관한 연구

김경석,장완식,김성식 한국공작기계학회 1997 한국생산제조학회지 Vol.6 No.2

The purpose of this study is the development of professional measuring system of piston. Designed values are compared with values of measured data of oval shape piston and allowable range and deviation are printed out by using this system. Thereafter, it is able to measure ovality precisely and to estimate itw quality rapidly.

충남대학교 부속동물병원에 의뢰된 개의 뇨결석 성분 분석 결과

노경완,송근호,조종기,박성준 忠南大學校 獸醫科大學 動物醫科學硏究所 2005 動物醫科學硏究誌 Vol.13 No.-

A retrospective analysis was performed with 14 cases of canine urolithiasis examined at Laboratory of Clinical Pathology, College of Veterinary Medicine, Chungnam National University in the period between January 2005 and November 2005. The female (8 cases) had most occurrence about 14 cases. The Shih-tzu (8 cases), Poodle (4 cases) had higher incidences than other breeds. Canine urolithiasis occurred from 1 year 3 months to 7 years 6 months, but the most prevalence age was 3 years. The major mineral component of calculi was calcium oxalate (64.28%) and struvite(21.44%).

中學校 學生들의 멘델유전에 관한 誤槪念 硏究

이경숙,이길재,정완호 한국교원대학교 과학교육연구소 1994 청람과학교육연구논총 Vol.4 No.1

Misconceptions of scientific concepts have been believed to be very persistent and difficult to be overcome through regular classroom activities. Therefore, it is important to find the misconceptions and cognitive structure of students. Mendelian genetics is an important and difficult topic for the student to learn. It is also difficult for the teacher to teach. Chromosome theory of inheritance and the concept of gene is the most important and difficult topics relate to mitosis/meiosis. The purpose of this research is a study of middle school students' understanding of the physical relationship of chromosome and genes. Particularly, this study is to establish new teaching strategy of the 3rd grade middle school students, by analyzing misconceptions of genetic continuity and to enhance their problem solving performance. Total 100 students were selected for this study from 2 different classes. They were all females. The test taken by students was open ended questionnaires. It was developed by Moll & Allen(1987) and it was modified for this study. Misconception was analyzed by personal interview. Each of the interviews was recorded and transcribed. Everything that students wrote and drew during the interviews, as well as the interview transcripts, were used to construct the data to be analyzed. The new instruction program "chromosome card game" used to reverse the misconceptions of Mendelian genetics, which were discovered in this study. The new instruction program was reviewed by having controlled experiment and treated additional study group. The results of this study are as follows: 1. Students have misconception on sister chromatids, the pairs of homologous chromosomes and gamete types. 2. According to the conceptual knowledge of chromosome and gene behavior, three types of chromosome model were suggested such as one-chromosome model, two-chromosome model and four-chromosome model. Many students can not construct the conceptual knowledge of genetics and meiosis. There is a tendency toward being mathematical rather than genetic in problem solving. 3. After the new instruction program was applied to the students, much enhanced study effect was discovered among experiment group. Based on above mentioned study, the classroom teachers must recognize the misconceptions some students have, and modify students' misconceptions, and increase the probability of meaningful learning.

여자중·고등학교 단거리선수의 100m 보폭 및 구간별 질주속도 분석

정완식,김정태,신재경 창원대학교 체육과학연구소 1999 경남 체육연구 Vol.4 No.2

본 연구의 목적은 100m의 거리를 10m씩 10개 구간으로 나누어 질주속도 및 평균 보폭을 측정한후, 구간별 질주속도와 가속도를 비교·분석하여 100m 달리기의 기록향상을 위한 과학적이고 합리적인 훈련 기초자료를 제공하는데 있다. 이를 수행하기 위하여 경상남도 소속 여자 중·고등학교 단거리선수 각 7명씩 총 14명을 대상으로 비디오 카메라, 비디오 편집기 타이머를 이용하여 평균 보폭, 단위구간별 속도 및 가속도, 구간별 속도 및 가속도의 기록을 분석하였다. 이상과 같은 과정을 통해 100m 질주속도를 분석한 결과는 다음과 같다. 1. 단위구간 중 최고속도가 나타난 구간은 중학교 선수는 30m 구간, 고등학교 선수는 40m 구간이었고, 중학교 선수는 50m 구간 이후에 속도가 감속되며, 고등학교 선수는 70m 구간 이후에 급격히 속도가 감속되었다. 2. 단위구간 중 가장 빠른 가속을 보인 구간은 중·고등학교 선수 공히 20m 구간이었고, 가장 큰 감속을 보인 구간은 중·고등학교 선수 공히 80m 구간이었다. 3. 구간별 속도는 중·고등학교 선수 공히 전속구간, 전속유지구간, 가속구간 순으로 빠르게 나타났다. 4. 구간별 가속의 빠르기는 중학교 선수는 가속구간, 전속유지구간, 전속구간 순이며, 고등학교 선수는 가속구간, 전속구간, 전속유지구간 순으로 나타났다. 5. 평균 보폭과 기록과는 중·고등학교 선수 공히 상관이 없는 것으로 나타났다. 6. 각 단위구간별 중·고등학교 선수간의 속도를 비교해 본 결과 전 구간에서 유의한 차이가 나타나지 않았다.

비젼 제어시스템에 사용된 카메라의 최적개수에 대한 실험적 연구

장완식,김경석,김기영,안힘찬 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.2

The vision system model used for this study involves the six parameters that permits a land of adaptability in th relationship between the camera space location of manipulable visual cues and the vector of robot joint coordinates is estimated in real he. Also this vision control method quires the number of cameras to transform 2-D camera plane from 3-D physical space, and be used irrespective of location of cameras, if visual cues are displayed in the same camera plane. Thus, this study is to investigate the optimal number of cameras used for the developed vision control system according to the change of the number of cameras. This study is processed in the two ways : a) effectiveness of vision system model b) optimal number of cameras. These results show the evidence of the adaptability of the developed vision control method using the optimal number of cameras.

한국어 어절 중의성의 출현 양상 : 어휘의 계량적 연구 방법을 통하여

남경완 우리어문학회 2002 우리어문연구 Vol.19 No.-

The purpose of this paper is to examine the aspects of ambiguity of word-clause on Korean through the method of measuring investigation. The concept of word-clause is the unique one of Korean caused by agglutinative feature. It means that one word-clause can be interpreted into several meaning, for example, the word-clause 'ganin(going)' composed by stem 'ga' and affix 'nin' can be interpreted into 'thin', 'gong' and 'grind' etc. By the way, the ambiguity of word-clause makes lots of difficulties to understand the meaning of sentence especially for foreign language learners and it also disturb the mechanistic processing of Korean in special branch like machinetranslation. So this study investigate the sentences containing ambiguous word-clause and look for the circumstance that determine the meaning of ambiguous word-clause. In the most cases, we can solve ambiguity by the information about the relation between the previous or next elements and ambiguous word-clause. The result of this study can be regarded as a fundamental data to distinct the ambiguity of word-clause.