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ORIGINAL PAPER : Inhibitory effects of juglanin on cellular senescence in human dermal fibroblasts
( Hyo Hyun Yang ),( Kyong Hwangbo ),( Ming Shan Zhang ),( Jong Keun Son ),( Hwa Young Kim ),( Suk Hwan Baek ),( Hyung Chul Choi ),( So Young Park ),( Jae Ryong Kim ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0
Cellular senescence contributes to tissue and organismal aging, tumor suppression and progress, tissue repair and regeneration, and age-related diseases. Thus, aging intervention might be a promising target for treatment and prevention of diverse age-related diseases. In the present study, we investigated whether juglanin purified from the crude extract of Polygonum aviculare exerted inhibitory activity against cellular senescence in human dermal fibroblasts (HDFs). Juglanin decreased senescence-associated β-galactosidase activity (SA-β-gal) and the level of reactive oxygen species in senescent cells induced by adriamycin treatment. Juglanin also repressed SA-β-gal activity in HDFs under replicative senescence. These results suggest that juglanin represses cellular senescence in HDFs and might be useful for the development of dietary supplements or cosmetics that alleviate tissue aging or age-related diseases.
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김원호(Won Ho Kim),이종경(Joung Kyong Lee),박형수(Hyung Soo Park),황보순(Soon Hwangbo),임영철(Young Cheol Lim),지희정(Hee Chung Ji),이효원(Hyo Won Lee),윤봉기(Bong Ki Yoon),서성(Sung Seo) 韓國草地學會 2009 한국초지조사료학회지 Vol.29 No.4
본 연구는 논에서 생육특성과 생산성이 우수한 두과 사료작물을 선발하기 위해서 천안(국립축산과학원)과 나주(전남도농업기술원) 답리작 논에서 2004부터 2006년까지 수행되었다. 두과 사료작물은 자운영, 크림손 클로버, 사료용 완두 그리고 헤어리 베치 초종을 공시하였고 각 초종의 품종은 자운영에서 국내에서 자생하는 품종(야생종)과 크림손 클로버 (C.V. Linkarus), 사료용 완두(C.V. Austroan pea), 헤어리 베치(C.V. Oregon common)을 각각 공시하였다. 나주지역에서 수행되었던 두과 사료작물의 개화기는 자운영이 가장 빠르고 Forage pea 초종이 가장 늦었다. 크림손 클로버 초종의 생초, 건물 그리고 조단백질 수량은 각각 28,870, 6,986 그리고 943 ㎏/㏊으로 가장 많았다. 그러나 자운영에서는 9,885, 1,749 그리고 338 ㎏/㏊으로 가장 적었다. 천안지역 논에서는 자운영이 가장 빠르고 헤어리 베치 초종에서 가장 늦었다. 헤어리 베치 초종의 생초, 건물 그리고 조단백질 수량은 각각 7,916, 1,141 그리고 113 ㎏/㏊으로 가장 많았고 자운영에서 270, 42 그리고 8 ㎏/㏊으로 가장 적었다. 조단백질 함량은 평균 14.3%로 높았고 자운영에서 19.3%로 가장 높았으나 헤어리 베치 초종에서 9.9%로 가장 낮았다. 따라서 논에서 생육특성과 수량을 고려한 결과 나주지여에서는 크림손 클로버 초종이 우수하고 천안지역에서는 헤어리 베치 초종이 우수하였다. This experiment was conducted to compare the agronomic characteristics and productivity of induced annual legumes at paddy field of Cheonan and Naju from 2004 to 2006. Chinese milk vetch (wild type), crimson clover (C.V. Linkarus), forage pea (C.V. Austrian pea), hairy vetch (C.V. Oregon commen) were used in this study. At the Naju region, the earliest flowering on 2nd may was found in Chinese milk vetch, while the latest on 18th May in forage pea. Fresh, dry matter (DM) and crude protein (CP) yields of crimson clover were highest as 28,870, 6,986 and 943 ㎏/㏊, respectively. but the yield of chinese milk vetch was low by 9,885, 1,749 and 338 ㎏/ha. At the Cheonan region, the most fast flowering date variety was Chinese milk vetch, hairy vetch was very late as 19th May. Fresh, DM and CP yields of hairy vetch were highest as 7,916, 1,141 and 113 ㎏/㏊, respectively. but the yield of chinese milk vetch showed the lowest by 270, 42 and 8 ㎏/㏊. The CP content of annual legumes was 14.3%. chinese milk vetch showed the highest CP content by 19.3% and hairy vetch showed the lowest content by 9.9%. The present data showed that the productivity was closely dependent on winter hardness, in conclusion, crimson clover at Naju and hairy vetch at Cheonan region was suggested to be the most proper species as winter crop after rice harvest on paddy field when considered the winter hardiness.
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( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
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Kyung Min Park,손종근,Ying Li,Bora Kim,Haiyan Zhang,Kyong Hwangbo,Dong Gen Piao,Mei Juan Chi,우미희,최재수,이제현,문동철,장현욱,김재룡 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12
Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among ‘Injinho’ and ‘Myeon-injin’ and ‘Haninjin’ – A. capillaris collected in autumn,A. capillaris collected in spring and A. iwayomogi, which can be misused as ‘Injinho’ in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40oC. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognition analysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.
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