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Park, Jang Won,Yun, Young-Pil,Park, Kyeongsoon,Lee, Jae Yong,Kim, Hak-Jun,Kim, Sung Eun,Song, Hae-Ryong Elsevier 2016 Colloids and surfaces Biointerfaces Vol.147 No.-
<P><B>Abstract</B></P> <P>The objectives of this study were (1) to fabricate ibuprofen-loaded porous microspheres (IBU/PMSs), (2) to evaluate the <I>in vitro</I> anti-inflammatory effects of the microspheres using LPS-induced inflammation in cultured synoviocytes, and (3) to evaluate the <I>in vivo</I> effect of the IBU/PMSs on the progression of monosodium iodoacetate (MIA)-induced osteoarthritis (OA) in a rat model. A dose-dependent <I>in vitro</I> anti-inflammatory effect on pro-inflammatory cytokine markers (matrix metallopeptidase-3 (MMP-3), matrix metallopeptidase-13 (MMP-13), cyclooxygenase-2 (COX-2), a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5)), interleukin-6 (IL-6), and tumor necrosis factor (TNF-α) was observed by confirming with real-time PCR analyses. <I>In vivo</I>, treatment with IBU/PMSs reduced MIA-stimulated mRNA expression of MMP-3, MMP-13, COX-2, ADAMTS-5, IL-6, and TNF-α in rat synoviocytes. In addition, we demonstrated that intra-articular IBU/PMSs suppressed the progression of MIA-induced OA in the rat model <I>via</I> anti-inflammatory mechanisms. In conclusion, IBU/PMSs are a promising therapeutic material to control the pain and progression of OA.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ibuprofen-loaded porous microspheres were fabricated by a fluidic device. </LI> <LI> Ibuprofen from IBU/PMSs was released in a sustained manner for 63 days. </LI> <LI> IBU/PMSs suppressed the progression of MIA-induced OA in the rat model. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Ibuprofen-loaded porous PLGA microspheres fabricated by a fluidic device effectively prevented the destruction of cartilages in MIA-induced OA joints.</P> <P>[DISPLAY OMISSION]</P>
Park, Ji Sun,Park, Kyeongsoon,Woo, Dae Gyun,Yang, Han Na,Chung, Hyung-Min,Park, Keun-Hong WILEY-VCH Verlag 2008 Small Vol.4 No.11
<B>Graphic Abstract</B> <P>The effective stabilization of 2- and 3D assemblies of heparin/poly(l-lysine) nanoparticles is achieved through embedment in DHEA- and DEXA-loaded poly(l-lactic-co-glycolic acid) (PLGA) microspheres (see image). These structures, which have great potential for use in cell delivery and for tissue regeneration, are manufactured using layer-by-layer electrostatic self-assembly, and are shown to have a positive effect on bone-marrow stromal cell aggregation and differentiation. <img src='wiley_img/16136810-2008-4-11-SMLL200701315-content.gif' alt='wiley_img/16136810-2008-4-11-SMLL200701315-content'> </P>
Heparin–deoxycholic acid chemical conjugate as an anticancer drug carrier and its antitumor activity
Park, Kyeongsoon,Lee, Gee Young,Kim, Yoo-Shin,Yu, Mikyung,Park, Rang-Woon,Kim, In-San,Kim, Sang Yoon,Byun, Youngro Elsevier 2006 Journal of controlled release Vol.114 No.3
<P><B>Abstract</B></P><P>A chemically modified heparin–DOCA (HD) conjugate was developed as a drug carrier for cancer therapy. HD conjugate was found to have markedly low anticoagulant activity and to form self-assembled nanoparticles in aqueous condition. We observed that HD conjugate prevented squamous cell carcinoma (SCC) and human umbilical vascular endothelial cell (HUVEC) proliferation during BrdU incorporation assays. Here, we prepared doxorubicin-loaded heparin nanoparticles by entrapping doxorubicin into the amphiphilic HD conjugate by physical interaction and characterized the properties of these nanoparticles using Dynamic Light Scattering (DLS) and Atomic Force Microscope (AFM). In this study, doxorubicin-loaded heparin nanoparticles were designed to improve the antitumor effects of nano-sized particles (range of 180 to 210?nm) at high drug-loading efficiencies in the range 64% to 96%. These doxorubicin-loaded heparin nanoparticles displayed sustained drug release patterns. It was confirmed in vivo toxicity studies that HD conjugate did not induce unexpected side effects and that DHN 20 was safer than free DOX. An in vivo study showed that HD conjugate, doxorubicin and DHN 20 (one of doxorubicin-loaded heparin nanoparticles) induced tumor volume reductions of 43%, 56% and 74%, respectively, relative to the saline treated control. These results suggest that the drug-entrapped with heparin nanoparticles might provide a novel therapy for SCC.</P>
Park, Kyeongsoon,Kim, Jong-Ho,Nam, Yun Sik,Lee, Seulki,Nam, Hae Yun,Kim, Kwangmeyung,Park, Jae Hyung,Kim, In-San,Choi, Kuiwon,Kim, Sang Yoon,Kwon, Ick Chan Elsevier 2007 Journal of controlled release Vol.122 No.3
<P><B>Abstract</B></P><P>To improve the <I>in vivo</I> tumor targeting characteristics of polymeric nanoparticles, three glycol chitosan (GC-20?kDa, GC-100?kDa, and GC-250?kDa) derivatives with different molecular weights were modified with cholanic acid at the same molar ratio. The resulting amphiphilic glycol chitosan–cholanic acid conjugates self-assembled to form glycol chitosan nanoparticles (GC-20?kDa-NP, GC-100?kDa-NP, and GC-250?kDa-NP) under aqueous conditions. The physicochemical properties of all three glycol chitosan nanoparticles, including degree of substitution with cholanic acid, surface charge, particle size and <I>in vitro</I> stability, were similar regardless of molecular weight. <I>In vivo</I> tissue distribution, time-dependent excretion, and tumor accumulation of glycol chitosan nanoparticles labeled with the near-infrared (NIR) fluorophore, Cy5.5, were monitored in SCC7 tumor-bearing mice, using NIR fluorescence imaging systems. Glycol chitosan nanoparticles displayed prolonged blood circulation time, decreased time-dependent excretion from the body, and elevated tumor accumulation with increasing polymer molecular weight. The results collectively suggest that high molecular weight glycol chitosan nanoparticles remain for longer periods in the blood circulation, leading to increased accumulation at the tumor site. Accordingly, we propose that enhanced tumor targeting by high molecular weight glycol chitosan nanoparticles is related to better <I>in vivo</I> stability, based on a pharmacokinetic improvement in blood circulation time.</P>
Park, Kyeongsoon,Lee, Ga Won Springer 2011 Nanoscale research letters Vol.6 No.1
<P>High-quality Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3 </SUB>nano-powders were synthesized by the solution combustion process. The size of the synthesized Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3 </SUB>powders was approximately 23 nm. The green pellets were sintered at 1150-1300°C at a step size of 50°C. Sintered Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3 </SUB>bodies crystallized in the perovskite structure with an orthorhombic symmetry. The sintering temperature did not affect the Seebeck coefficient, but significantly affected the electrical conductivity. The electrical conductivity of Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3 </SUB>increased with increasing temperature, indicating a semiconducting behavior. The absolute value of the Seebeck coefficient gradually increased with an increase in temperature. The highest power factor (3.7 × 10<SUP>-5 </SUP>Wm<SUP>-1 </SUP>K<SUP>-2 </SUP>at 800°C) was obtained for Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3 </SUB>sintered at 1,250°C. In this study, we investigated the microstructure and thermoelectric properties of Ca<SUB>0.8</SUB>Dy<SUB>0.2</SUB>MnO<SUB>3</SUB>, depending on sintering temperature.</P>
Park Ji Sun,Kim Young-Woo,Kim Hyungdong,Kim Sun-Ki,Park Kyeongsoon 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.11
Quantitative analysis of adenosine triphosphate (ATP) has been widely used as a diagnostic tool in the food and medical industries. Particularly, the pathogenesis of a few diseases including inflammatory bowel disease (IBD) is closely related to high ATP concentrations. A bioluminescent D-luciferin/luciferase system, which includes a luciferase (FLuc) from the firefly Photinus pyralis as a key component, is the most commonly used method for the detection and quantification of ATP. Here, instead of isolating FLuc produced in recombinant Escherichia coli, we aimed to develop a whole-cell biocatalyst system that does not require extraction and purification of FLuc. To this end, the gene coding for FLuc was introduced into the genome of probiotic Saccharomyces boulardii using the CRISPR/Cas9-based genome editing system. The linear relationship (r2 = 0.9561) between ATP levels and bioluminescence generated from the engineered S. boulardii expressing FLuc was observed in vitro. To explore the feasibility of using the engineered S. boulardii expressing FLuc as a whole-cell biosensor to detect inflammation biomarker (i.e., ATP) in the gut, a colitis mouse model was established using dextran sodium sulfate as a colitogenic compound. Our findings demonstrated that the whole-cell biosensor can detect elevated ATP levels during gut inflammation in mice. Therefore, the simple and powerful method developed herein could be applied for non-invasive IBD diagnosis.
Park, Kyeongsoon,Hakeem, Deshmukh Abdul,Cha, Jae Sung The Royal Society of Chemistry 2016 Dalton Transactions Vol.45 No.16
<P>A series of Ca3-xAgxCo4O9+delta (0 <= x <= 0.2) powders is prepared by the Pechini sol-gel method. The effect of dispersants on the size and morphology of the Ca2.9Ag0.1Co4O9+delta powders is investigated. The desired powders are obtained after calcinations of the dried powders at 800 degrees C for 12 h. The structural and morphological properties are studied with the help of XRD patterns, FE-SEM images, FT-IR spectra, Raman spectra, and XPS spectra. It is found that a mixed valence (+ 3 and + 4) of Co exists in a CdI2-type CoO2 layer, while a mixed valence (+ 2, + 3, and + 4) exists in the CoO of a rock salt-type Ca2CoO3 layer. The mixed-valence state in the CoO2 layer can improve the high-temperature thermoelectric properties of Ca3Co4O9 systems.</P>
Kyeongsoon Park(박경순) 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
Macrophage-derived foam cells paly critical roles in the development of atherosclerosis. Thus, they are considered as important target biomarkers for atherosclerosis therapy. Our group has developed a macrophage mannose receptor (MMR)-targeted nanodrug loaded with lobeglitazone (MMR-Lobe). The MMR-Lobe had a high binding affinity to foam cells, and it could efficiently promote cholesterol efflux via LXRα, ABCA1, and ABCG1 pathways, and inhibit pro-inflammatory mediators. Using optical diffraction tomography, we identified lipid droplets in foam cells and quantitatively evaluated the therapeutic effects of MMR-Lobe. Also, long-term delivery of MMR-Lobe markedly reduced both plaque burden and inflammation in atherogenic mice without undesirable systemic effects. Furthermore, short-term treatment of MMR-Lobe showed a robust acute anti-inflammatory effect on inflamed plaque in coronary-sized arteries and shifted the plaque composition to a stable phenotype.