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In situ induction of dendritic cell–based T cell tolerance in humanized mice and nonhuman primates
Jung, Kyeong Cheon,Park, Chung-Gyu,Jeon, Yoon Kyung,Park, Hyo Jin,Ban, Young Larn,Min, Hye Sook,Kim, Eun Ji,Kim, Ju Hyun,Kang, Byung Hyun,Park, Seung Pyo,Bae, Youngmee,Yoon, Il-Hee,Kim, Yong-Hee,Lee, The Rockefeller University Press 2011 The Journal of experimental medicine Vol.208 No.12
<P>Induction of antigen-specific T cell tolerance would aid treatment of diverse immunological disorders and help prevent allograft rejection and graft versus host disease. In this study, we establish a method of inducing antigen-specific T cell tolerance in situ in diabetic humanized mice and Rhesus monkeys receiving porcine islet xenografts. Antigen-specific T cell tolerance is induced by administration of an antibody ligating a particular epitope on ICAM-1 (intercellular adhesion molecule 1). Antibody-mediated ligation of ICAM-1 on dendritic cells (DCs) led to the arrest of DCs in a semimature stage in vitro and in vivo. Ablation of DCs from mice completely abrogated anti–ICAM-1–induced antigen-specific T cell tolerance. T cell responses to unrelated antigens remained unaffected. In situ induction of DC-mediated T cell tolerance using this method may represent a potent therapeutic tool for preventing graft rejection.</P>
Effect of button mushroom compost on mobilization of heavy metals by sunflower
Kyeong, Ki-Cheon,Kim, Yong-Gyun,Lee, Chan-Jung,Lee, Byung-Eui,Lee, Heon-Hak,Yoon, Min-Ho 한국버섯학회 2014 한국버섯학회지 Vol.12 No.3
The potential ability of Button mushroom compost (BMC) to solubilize heavy metals was estimated with metal contaminated soils collected from abandoned mines of Boryeong area in South Korea. The bacterial strains in BMC were isolated for investigating the mobilization of metals in soil or plant by the strains and identified according to 16S rRNA gene sequence analysis. When metal solubilization potential of BMC was assessed in a batch experiment, the BMC was found to be capable of solubilizing metals in the presence of metals (Co, Pb and Zn) and the results showed that inoculation of BMC could increase the concentrations of water soluble Co, Pb and Cd by 35, 25 and 45% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 27, 25 and 28% respectively in Co, Pb and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction of Co, Pb and Zn from contaminated soils.
Kyeong-Cheon Jung,Byung Hyun Kang,Hyo Jin Park,Hi Jung Park,Jae-il Lee,Seong Hoe Park 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.6
PLZF-expressing invariant natural killer T cells and CD4 T cells are unique subsets of innate T cells. Both are selected via thymocyte-thymocyte interaction, and they contribute to the generation of activated/memory-like CD4 and CD8 T cells in the thymus via the production of IL-4. Here, we investigated whether PLZF+ innate T cells also affect the development and function of Foxp3+ regulatory CD4 T cells. Flow cytometry analysis of the thymus and spleen from both CIITA transgenic C57BL/6 and wild-type BALB/c mice, which have abundant PLZF+ CD4 T cells and invariant natural killer T cells, respectively, revealed that Foxp3+ T cells in these mice exhibited a CD103+ activated/memory-like phenotype. The frequency of CD103+ regulatory T cells was considerably decreased in PLZF+ cell-deficient CII-TATgPlzflu/lu and BALB/c.CD1d−/− mice as well as in an IL-4-deficient background, such as in CIITATgIL-4−/− and BALB/ c.IL-4−/− mice, indicating that the acquisition of an activated/memory-like phenotype was dependent on PLZF+ innate T cells and IL-4. Using fetal thymic organ culture, we further demonstrated that IL-4 in concert with TGF- enhanced the acquisition of the activated/memory-like phenotype of regulatory T cells. In functional aspects, the activated/memory-like phenotype of Treg cells was directly related to their suppressive function; regulatory T cells of CIITATgPIV-/- mice more efficiently suppressed ovalbumin-induced allergic airway inflammation compared with their counterparts from wild-type mice. All of these findings suggest that PLZF+ innate T cells also augmented the generation of activated/memory-like regulation via IL-4 production.
Choi, Yun Jung,Park, Hi-Jung,Park, Hye Jin,Jung, Kyeong Cheon,Lee, Jae-Il Hindawi 2018 Journal of immunology research Vol.2018 No.-
<P>Peripheral CD4/CD8 double-positive (DP) T cells are associated with autoimmune disorders, cancer, and viral infection. However, the relationship between organ transplantation and DP T cells is unclear. Here, we examined the functional characteristics of peripheral DP T cells and analyzed their significance with respect to islet graft rejection in a nonhuman primate model of islet transplantation. DP T cells were functionally equivalent to conventional CD4 and CD8 T cells in terms of helper and cytotoxic activity, respectively. DP T cells expressed high levels of CXCR5 and PD-1 and secreted IFN-<I>γ</I>, IL-4, and IL-21 in amounts equivalent to those secreted by CD4 or CD8 T cells; also, they produced large amounts of granzyme B and perforin. In addition, under steady-state conditions, DP T cells expressed eomesodermin (Eomes) and promyelocytic leukemia zinc finger (PLZF) proteins, both of which act as transcription factors in innate/memory-like T cells. The number of peripheral DP T cells in the islet transplantation model was high in the group that experienced graft rejection; this was not the case in the long-term survival group. Interestingly, numbers of effector memory T cells (TEM) within the DP T cell population increased significantly during islet graft rejection, as did those of TEM within the cytotoxic CD8 T cells. Furthermore, the most conspicuous of which was the increase of CD4<SUP>hi</SUP>CD8<SUP>low</SUP> T cell subpopulation at that point. Taken together, the data suggest that peripheral DP T cells showing an innate/memory-like phenotype have both helper and cytotoxic activity <I>in vitro</I> and that they may act as a novel biomarker for graft rejection after islet transplantation.</P>
Effect of button mushroom compost on mobilization of heavy metals by sunflower
Kyeong, Ki-Cheon,Kim, Yong-Gyun,Lee, Chan-Jung,Lee, Byung-Eui,Lee, Heon-Hak,Yoon, Min-Ho The Korean Society of Mushroom Science 2014 한국버섯학회지 Vol.12 No.3
The potential ability of Button mushroom compost (BMC) to solubilize heavy metals was estimated with metal contaminated soils collected from abandoned mines of Boryeong area in South Korea. The bacterial strains in BMC were isolated for investigating the mobilization of metals in soil or plant by the strains and identified according to 16S rRNA gene sequence analysis. When metal solubilization potential of BMC was assessed in a batch experiment, the BMC was found to be capable of solubilizing metals in the presence of metals (Co, Pb and Zn) and the results showed that inoculation of BMC could increase the concentrations of water soluble Co, Pb and Cd by 35, 25 and 45% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 27, 25 and 28% respectively in Co, Pb and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction of Co, Pb and Zn from contaminated soils.
A Study on the Pizza Restaurants Customer Satisfaction a Priority Using AHP
Kyeong Kyoo Wang,Chung Soon Suk,Shin Cheon Sik,Yoo Ji Chul,Lee Mi Jung 한국상품학회 2016 商品學硏究 Vol.34 No.6
This study is currently pizza restaurants in saturated unprofessional targets. pizza restaurants in Korea General 20-30 consumers to quantify the importance of selecting attributes for a pizza restaurants, a pizza restaurants in korea, the pizza market competition by sequencing to the executive management and want to operate a pizza restaurant operation intended to provide a strategic implications to support the safe execution. To this end, a pizza restaurants and a relevant extract a wide range of literature significant selection through attributes to existing research , and a pizza restaurantsmonth aimed at about 20 to 30 consumers visited at least oncewas survey , the result of the survey AHP importance of pizza restaurants selected by the properties investigated and priority ranking. The questionnairewas deployed to 237 , the same number was recovered. The questionnairewas 37 except for a survey that determined that the response to insincerity, was also analyzed using a total of 200 respondents , excluding Expert Choice 11.5 programs do notmeet the above criteria.