Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

재생PE/폐타이어분말 블렌드의 특성

계형산,이응옥 목원대학교 자연과학연구소 2002 自然科學 硏究論文集 Vol.11 No.2

본 연구는 급증하고 있는 폐타이어 처리 문제를 해결하기 위한 연구의 일환으로서 플라스틱수지(재생PE. HDPE)를 폐타이어분말(WTP)과 블렌드하였을때 폐타이어분말 함량에 따른 물성의 변화에 관한 연구 논문이다. 본 연구에서는 플라스틱수지(재생PE, HDPE)와 폐타이어 분말의 혼합비를 0∼50 wt.%로 하였다. 본 연구에서는 치합형 동방향 회전 이축 압출기(Fully intermeshing co-rotating twin screw extruder)를 이용하여 각 조성별로 블렌드를 하였으며 ASTM에 의거하여 인장강도, 충격강도 등의 물성 변화를 측정 하였고, Capillary Rheometer를 이용하여 재생PE/폐타이어분말 블렌드의 전단율의 변화에 따른 용융점도의 변화와 연화점(Ts)등의 유변학적 성질을 분석하였다.

Polycarbonate, PBT, 충격 보강재 등의 이성분계와 삼성분계 블렌드의 기계적 성질 및 유변학 특성

계형산,최종규 목원대학교 자연과학연구소 2002 自然科學 硏究論文集 Vol.11 No.2

본 연구는 Polycarbonate(PC) 와 Polybutylene Terephthalate(PBT)의 블렌드시 충격 보강재의 첨가에 따른 각 조성별 블랜드의 유동성 및 기계적 특성의 변화에 대한 연구이다. 본 연구에서는 치합형 동방향 회전 이축 압출기를 사용하여 조성별 블렌드를 하였고. ASTM에 의거하여 인장, 충격, 굴곡 등의 기계적 특성을 연구하였다. 또한 Capillary rheometer를 사용하여 Shear rate에 따른 점도의 변화와 연화점 및 온도에 따른 유변학적 성질을 분석하였다. 또한 이성분계 블렌드의 기계적 성질을 높이기 위해 부타디엔계 충격 보강재와 폐타이어 분말을 사용하였다. 각 조성별로 살펴본 기계적 특성은 PC/PBT 블렌드에 부타디엔계 충격 보강재를 첨가한 경우 기계적 강도가 증가함을 볼 수가 있었다. 그러나 폐타이어 분말을 첨가한 재료는 폐타이어 함량이 증가할수록 기계적 강도가 급격히 떨어지는 현상을 나타내었다. 각 성분별 연화점은 PC/PBT블랜드가 가장 높았고, 부타디엔계 충격 보강재, 폐타이어 분말의 순서를 나타내었다. 블렌드의 점도를 살펴보면 PC/PBT 블렌드의 점도가 WTP를 첨가한 것의 점도 보다 크게 향상되었으며, 따라서 가공성이 저하 됨을 알수 있었다. 밤면 부타디엔계 충격 보강재를 첨가한 경우의 점도는 PC/PBT 블랜드보다 초기 값은 높으나, 어느정도 Shear가 높아지게 되면 역전이 되는 현상이 나타났다.

재생 폴리에텔렌의 기계적 성질 : 충격 보강재 효과에 대하여

계형산,강경민,박종훈,정융권,이상환 목원대학교 자연과학연구소 2002 自然科學 硏究論文集 Vol.11 No.2

본 연구는 재활용이 가능한 폴리에틸렌 필름 등에 비산회(Fly Ash) 등의 충진재를 사용하여, 신재와 물성을 비교하여 재생 PE의 재활용 가능성 및 사용 가능한 충진재의 종류를 알아보는데 그 목적이 있다. 또한 본 연구에서는 치합형 동방향 회전 이축 압출기(Fully Intermeshing Co-rotating Twin Screw Extruder)를 이용해서 재생 Polyethylene에 비산회 및 다양한 충진재(filler)를 첨가하여 ASTM에 의거하여 충격시편을 만들었고, 각 충진재의 무게비에 따른 충진재 충격 시험을 하였으며 Capillary Rheometer를 이용하여 유변학적인 성질을 분석해 보았다.

변형된 Radial 기반 신경회로망을 이용한 다중 물체 검출 및 세그멘테이션

金柱慶,全俊亨,金鎭浩 慶一大學校 1996 論文集 Vol.13 No.2

본 논문에서는 변형된 RBF 신경회로망을 이용하여 다중 물체에 대한 사전 정보 없이도 효과적으로 다중 물체를 검출 및 분리할 수 있는 알고리즘을 제안하였다. 제안된 알고리즘에서는 대칭성을 잘 반영하는 RBF 신경회로망으로 GST(generalized symmetry transform)를 구현하기 위해 서로 대칭인 위치에 존재하는 화소들이 에지 방향성분 차이를 MRBNN의 입력으로 하고, 대칭인 화소간 거리의 함수로 가중치를 설정함으로써 대칭성이 충분히 반영될 수 있도록 하였다. 또한, 입력영상에 대한 경계선 추출 영상을 피라미드 구조로 계층화 한 후, 피라미드 구조의 각 단계에 MRBNN을 적용함으로서 고정된 크기의 대칭 마스크로도 물체 검출 및 분리가 동시에 가능하도록 하였다 여러 물체가 혼재하는 실영상에 대해 실험해 본 결과 물체의 중심 추출을 통한 물체검출 및 물체 분리가 효과적으로 이루어짐을 확인할 수 있었다.

여성생식계 상피세포에서 Glutathione S-Transferase 출현도에 관한 면역조직화학적 관찰

송계용,김석중,김미경,박용욱,유재형,박상철 중앙대학교 의과대학 의과학연구소 1991 中央醫大誌 Vol.16 No.2

Both placental form (GST-Pi) and basic form (GST-L) of glutathione S-transferase were studied in the epithelium of normal, tumor and tumor like lesions of female genital system. The results obtained were as follows : 1. Staining reaction of GST-Pi were moderate in normal cytotrophoblasts, mild in syncytiotrophoblasts, strong in corpus luteum, moderate to strong in salpingeal mucosa and in proliferative glands, moderate in secretory glands of indometrium and moderate in superficial squamous epithelium of cervix 2. Staining reaction of GST-L were mild in cytotrophoblasts and in syncytiotrophoblasts, of chorionic villi in corpus luteum, negative in salpingeal mucosa, very weak in proliferative glands, mild in secretory, glands of endometrium and in superficial squamous epithelium of cervix. 3. Staining reaction of GST-L were mild in cytotrophoblast and syncytiotrophoblast of choriocarcinoma, moderate in cytotrophoblasts, mild in syncytiotrophoblasts of hydatidiform mole, negative in mucinous epithelium of cust adenoma, in hyperplastia and adenocarcinoma, and mild in adenocarcinoma of cervix. 4. Staining reaction of GST-L were very weak in cytotrophoblasts and syncytiotrophoblasts of choriocarcinoma and in hydatidiform mole, negative in mucinous epithelium of cust adenoma, very weak in serous eptiethlium, negative to mild in hyperplastic and adenocarcinoma, mild in squamous cell carcinoma and adenocarcinoma of cervix. Therefore, staining reaction and distribution of GST-Pi and L isoengumes were different in normal and neoplastic cell of trophoblasts, salpinx, endometrin and cervix

일렬 자가조혈모세포이식을 받은 다발성 골수종 환자에서 발생한 뇌 톡소포자충증 1례

김계형,송경호,전재현,박완범,박상원,김홍빈,김남중,김인호,오명돈 대한감염학회 2010 감염과 화학요법 Vol.42 No.3

Toxoplasmosis is a rare but fatal complication in hematopoietic stem cell transplant recipients, usually associated with allogeneic hematopoietic stem cell transplantation (HSCT). We report a case of cerebral toxoplasmosis in a patient with multiple myeloma, following tandem autologous stem cell transplantation. A 55-year-old Korean male presented with weakness in both legs that had progressed to both arms. A magnetic resonance imaging scan of the brain revealed multiple, variablesized ring-enhancing lesions with surrounding edema in the cerebral hemispheres and brain stem. Stereotactic biopsy revealed bradyzoites of Toxoplasma gondii in the brain tissue. The patient received trimethoprim-sulfamethoxazole, followed by pyrimethamine and sulfadiazine, accompanying treatment for progressive multiple myeloma. Cerebral toxoplasmosis should be considered as one of the differential diagnoses in patients with neurologic signs following autologous HSCT.

필리핀 여행 후 발생한 쯔쯔가무시병 1예

김계형,김낙현,김문석,김충종,전재현,박완범,장원종,박상원,김익상,오명돈,최강원 대한감염학회 2008 감염과 화학요법 Vol.40 No.6

Infectious diseases imported from other countries have increased as more and more Koreans are going abroad for various purposes. Tsutsugamushi disease from other endemic area such as Southeast Asia is important, because it can occur in any season and eschar may be absent. We report a case of imported tsutsugamushi disease acquired in the Philippines. A patient presented with fever, headache, and maculopapular skin rash. However, eschar was absent. Polymerase chain reaction (PCR) for 56-kDa gene of Orientia tsutsugamushi using buffy coat was positive. Serum indirect immunofluorescent antibody assay was initially negative but became positive with a titer of 1:320 at follow-up, Sequencing analysis revealed the strain to be 100% identical to the TW73R strain identified in Taiwan. After the patient received doxycycline, body temperature normalized in 12 hours. Tsutsugamushi disease is one of the differential diagnoses that should be included for patients with fever who have recently returned from Southeast Asian countries. PCR for O. tsutsugamushi using patient's buffy coat was useful for early diagnosis.

戰鬪地域에서 發生한 踵骨骨折에 對한 臨床的 考察 : 33例에 對한 分析

徐雄吉,趙桂衡,朴聖德 최신의학사 1970 最新醫學 Vol.13 No.2

The clinical analysis 33 cases of the calcaneal fractures, treated at the Dept. of Orthopedic Surgery of Chin-Hae Naval Hospital for three years from September, 1966 to August, 1969, was reported. The results were as follows; 1) All the patient were male and in the 3 rd. decade of life. 2) There were 20 cases caused by booby trap explosion, 9 cases by gunshot, 3 cases by falling from a height and one case by other causes. The most common cause in this series was the booby trap explosion as 60% in frequency. 3) 21 cases were open fractures and 12 cases were closed fractures. Open fractures were about twice as frequent as closed fractures. 4) The classification in this series was follwed to Essex-Lopresti's; According to his classification, Group I was fractures of the calcaneus not involving the subtalar joint and Group II was -fractures of the calcaneus involving the subtalar joint. 5) Associated injuries were in 22 cases (66%). Among them, 19 cases were caused by booby trap explosion. Comminuted fractures of the tibia and the fibula in 10 cases, comminuted fractures of the femur in 5 cases, loss of soft tissue of heel in 3 cases, injuries of common peroneal nerve in 2 cases, fractures of the metatarsal bone in one case and fracture of the second lumbar vertebra in one case, could be noticed as associated injuries. It is said that fractures of the calcaneus caused by falling from a height were frequently associated with fracture of the spine. But in our series, fracture of the spine could not be observed in the cases caused by booby trap explosion. 6) The principle of treatment in our series was as follows; Conservative treatment for Group I and open reduction or athrodesis for Group II. 7) Disabilities after treatment were 14 cases, in which 10 cases were treated by conservative method and 4 cases by open method. In our series, disability was found more frequently in the cases what had been treated by conservative method than in the cases treated by open method.

다발성 대장암 간전이 환자의 간절제를 위한 새로운 시도

주종우,김형철,임철완,신응진,조규석,유기원,송옥평,홍대식,박성진,조준희,이혜경,김희경,권계원,고은석 순천향의학연구소;Soonchunhyang Medical Research Institute 2004 Journal of Soonchunhyang Medical Science Vol.10 No.2

Multiple bilobar liver matastases (MBLM) are the main cause of low resectability in the colon cancer liver metastases. The authors experienced one case of initially non-resectable colon cancer liver metastases. He was curatively and safely treated with a two-stage hepatectomy using the new method of future remnant liver volume growing. A 54-year-old man was referred to our department with the sigmoid colon cancer combined with MBLM, which were checked in two small metastatic lesions in the left lobe and five large sized lesions in the right lobe in the computed tomogram (CT). A laparoscopic assisted anterior resection was primarily performed. We performed the 1^(st) stage hepatectomy 3 weeks after the colon resection. Intra-operative Ultrasonogram (US) found 9 small superficial metastatic lesions in the left lobe. All that lesions were completely removed by non-anatomical wedge resection. An occlusion ballon catheter was placed in the right portal vein through a small branch of the inferior mesenteric vein at that time. The future remnant liver volume was sufficiently increased 3 weeks after the 1^(st) hepatectomy. A right hepatectomy was safely performed 22 days after the 1^(st) hepatectomy. The patient received a regional chemotherapy (interleukin2 based immuno-chemotherapy through hepatic artery) for 4 months, then received 9 cycles of systemic chemotherapy (biweekly Oxaliplation, leucovorin, plus 5-fluorouracil) without any recurrence evidence.