Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

Biosimilar Development And Case Study in Celltrion

Ki?Sung Kwon 한국생물공학회 2010 한국생물공학회 학술대회 Vol.2010 No.10

1930년대 야담잡지 소재 연성흠의 야담 기술과 의미

권기성(Kwon, Ki-Sung) 우리문학회 2023 우리文學硏究 Vol.- No.79

1920년대부터 야담은 동화와 긴밀히 접촉하고 있었다. 다만 그 방법은 동화가 야담을 활용하는 정도였으며, 연구자의 관심도 여기에서 벗어나지 않았다. 그런데 1930년대부터 야담 잡지의 기획자는 동화작가들을 소환하여 그들이 지닌 소재의 유사성 등을 활용하여 야담을 작성하도록 한다. 그 차이를 살피기 위해 본고에서는 연성흠이 『월간야담』과 『야담』 잡지에 남긴 작품에 주목했다. 연성흠의 야담은 대개 전시기 야담집에 수록된 작품을 준용하는 특징을 보이지만, 필요한 경우가 있다면 적극적 변개도 주저하지 않았다. 그리고 이런 편찬과 서술의 특징은, 한결같이 선한 인물의 시혜와 보은이라는 지점을 강조하면서 ‘공동체 윤리’를 강조한 주제적 지향을 견인했다. 이는 그의 동화작법과 퍽 유사한 것이었다. 한편 야사를 토대로 새로운 이야기를 만들어낼 때는 통속과 비극이라는 당대의 ‘애사’ 글쓰기를 답습하는 경향도 보였다. 곧 야담과 야사의 글쓰기 방식이 미세하게 달랐던 셈이다. 논문에 제시된 연성흠의 작품을 통해 두 잡지의 기획이 다양한 형태로 이루어졌으며, 독자를 유인하는 기획의 소산이었음을 짐작해 볼 수 있었다. 이는 곧 야담 집필진 저변의 다양화를 통해 활로를 모색한 가운데, 아동문학과의 접속이 주요한 역할을 했던 것이다. 그 저변에는 지면의 확대에 따른 다양한 글쓰기의 가능, 개인의 경제적 사정, 친분 등 여러 이유가 있었다. 결과적으로 30년대 동화작가들이 야담 잡지에 참여하고 다양한 야담 기법을 시도하면서, 당시 야담의 흥행을 견인하는 일익을 담당하고 있었던 것이라 할 수 있다. Since the 1920s, Yadam has been in close contact with fairy tales. However, the method was to the extent that fairy tales used night stories, and the researcher’s interest did not deviate from this. However, from the 1930s, the organizers of Yadam magazine summoned fairy tale writers to write Yadam by utilizing the similarity of their materials. In order to examine the difference, this paper focused on the works left by Yeon Sung-heum in 『Wolgan-Yadam』 and 『Yadam』 magazines. Yeon Sung-heum’s Yadams usually show the characteristics of applying mutatis mutandis to the works contained in the exhibition’s Yadams, but he did not hesitate to actively transform himself if necessary. And these characteristics of compilation and description drove the thematic orientation that emphasized “community ethics” while emphasizing the point of the benefit and complement of good characters. This was very similar to his fairy tale writing. On the other hand, when creating a new story based on wild events, there was also a tendency to follow the writing of the “Sad-history” of the time of popularity and tragedy. In other words, the writing methods of Yadam and Yasa were slightly different. Through the work of Yeon Seong-heum presented in the paper, it was possible to guess that the planning of the two magazines was carried out in various forms, and that it was the product of the planning that attracted readers. In other words, the connection with children’s literature played a major role while seeking a way out through the diversification of the base of the Yadam writers. There were various reasons at the base, such as the possibility of various writing due to the expansion of the space, individual economic circumstances, and friendship. As a result, it can be said that in the 30s, fairy tale writers participated in Yadam magazines and tried various Yadam techniques, playing a role in driving the success of Yadam at that time.

A subpopulation of cancer stem cells identifies radiographic characteristics in glioblastoma

Kim, Ja Eun,Kim, Sung Kwon,Shin, Jaekyung,Se, Young-Bem,Choi, Seung Hong,Park, Sung-Hye,Choi, Seung Ah,Lee, Ji Yeoun,Phi, Ji Hoon,Wang, Kyu-Chang,Park, Chul-Kee,Kim, Seung-Ki D.A. Spandidos 2017 Oncology letters Vol.13 No.3

<P>Cancer stem cells (CSCs), defined by CD133 expression, harbor heterogeneous subpopulations of cells, including endothelial progenitor cells (EPCs). This study aimed to investigate whether a subpopulation of CSCs could affect the radiographic characteristics of glioblastoma. Tissue samples from 10 patients newly diagnosed with glioblastoma were selected according to the radiographic characteristics of their tumors. The patients were divided into two groups based on preoperative magnetic resonance imaging demonstrating contrast enhancement, necrosis and infiltrative patterns: the enhancement/necrosis group (E/N, n=5) and the non-enhancement/infiltration group (NE/I, n=5). Flow cytometry was used to assess the CSCs while immunohistochemistry was used to study microvessel density and the proliferation index. The EPC (CD34<SUP>+</SUP>/CD133<SUP>+</SUP>) fraction in CSCs (CD133<SUP>+</SUP>) was larger in the NE/I group. However, there was little difference in the angiogenic activity assessed using microvessel density between the two groups. The proliferation index (assessed using the antibody Ki-67) was higher in the E/N group and was negatively correlated with the EPC fraction. The non-EPC (CD34<SUP>−</SUP>/CD133<SUP>+</SUP>) fraction is a major factor responsible for radiographic characteristics of contrast enhancement, thus establishing an association between a subpopulation fraction of CSCs and radiographic characteristics in glioblastoma. Therefore, the simple non-invasive assessment of studying contrast enhancement lesions in glioblastomas may be used to estimate CSC subpopulations.</P>

불면을 호소하는 뇌실질내출혈 환자 치험 1례

김경묵,김세원,서유나,정우상,문상관,진철,조기호,권승원,Kim, Gyeong-muk,Kim, Se-won,Seo, Yu-na,Jung, Woo-sang,Moon, Sang-Kwan,Jin, Chul,Cho, Ki-ho,Kwon, Seungwon 대한중풍순환신경학회 2019 대한중풍.순환신경학회지 Vol.20 No.1

A case of a 46-year-old Korean male with post-stroke insomnia that induced by intracranial hemorrhage was presented. He was treated with Transcutaneous electric acupoint stimulation (TEAS) at BL62 and KI6 once per a day for 17 days. We recorded the patient's sleep time and time of asleep, and used PSQI-K to assess severity of patient's insomnia symptom. After using TEAS, there was improvement in patient's insomnia severity and the time of sleep. From the result of this case, electrical stimulation at acupoint can may be effective in treating post-stroke insomnia.

양 족부 열감을 호소하는 하지불안증후군 환자 치험 1례

김경묵,서원주,김수빈,정우상,문상관,권승원,조기호,Kim, Gyeong-muk,Suh, Won-joo,Kim, Su-bin,Jung, Woo-sang,Mun, Sang-kwan,Kwon, Seung-won,Cho, Ki-ho 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.2

Objectives: The purpose of this study is to evaluate the effectiveness of electroacupuncture on heat sensation of the feet of a 61-year-old female patient with restless leg syndrome. Methods: Electroacupuncture was performed at four acupoints (KI3 (太谿), KI6 (照海), LR3 (太衝), LR2 (行間)) selected to reduce heat sensation in both feet. The patient's symptoms were assessed using the VAS (Visual Analogue Scale), the SF-MPQ (short-form McGill Pain Questionnaire), and the IRLS (International Restless Legs Scale). The treatment was performed from April 20, 2010 to April 24, 2010. Results: During five sessions of electroacupuncture treatments, the VAS, SF-MPQ, and IRLS scores improved throughout the treatment period and afterwards. Conclusions: The results suggest that electroacupuncture at KI3(太谿), KI6(照海), LR3(太衝), LR2(行間) can be effective for reducing hot flush in both feet.

폐결핵으로 오인된 역형성 거대세포 림프종 1례

현동우,박재용,강태경,박기수,박태인,김창호,손상균,권건영,이규보,정태훈,권삼,Hyun, Dong-Woo,Park, Jae-Yong,Kang, Tae-Kyung,Park, Ki-Soo,Park, Tae-In,Kim, Chang-Ho,Sohn, Sang-Kyun,Kwon, Kun-Young,Lee, Kyu-Bo,Jung, Tae-Hoon,Kweon, Sam 대한결핵및호흡기학회 1998 Tuberculosis and Respiratory Diseases Vol.45 No.1

저자들은 발열, 체중감소 등의 증상과 흉부 X-선 사진상 폐경화의 소견이 있었던 환자에서 폐의 병변이 역형성 거대세포 림프종으로 추측되는 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Anaplastic large cell lymphoma(ALCL) is a recently recognized large cell lymphoma characterized by large pleomorphic cells, with prominent nucleoli, expressing the CD30(Ki-1) antigen. It is often misdiagnosed as metastatic carcinoma, malignant histiocytosis or Hodgkin's disease. Primary pulmonary ALCL is extremely rare although it frequently involves extranodal sites, e.g. skin, bone, gastrointestinal tract, soft tissue and lung. We report a case of ALCL suspected as primarily involving lung.

Poster Session 2 : Blocking metastasis with a synthetic molecule that inhibits tumor cell migration

( Ki Deok Shin ),( Sang Ku Lee ),( Kwang Hee Son ),( Hwan Mook Kim ),( Young Ki Paik ),( Byoung Mog Kwon ),( Dong Cho Han ) 한국생화학분자생물학회 (구 한국생화학회) 2005 생화학분자생물학회 춘계학술발표논문집 Vol.2005 No.-

Development of transgenic tall fescue plants expressing 2-Cys peroxiredoxins gene with enhanced tolerance to oxidative stress

Sang Yeol Lee,Byung Hyun Lee,Suk Yoon Kwon,Sang Soo Kwak,Ki Won Lee,Kyung Hee Kim,Dong Gi Lee,Choong Hoon Park,Iftekar Alam,Nagib Ahan 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.2007 No.6

Herbicide resistant transgenic tall fescue plants produced by an efficient Agrobacterium tumefaciens transformation method

Ki Won Lee,Sang Hoon Lee,Ki Yong Kim,Gi Jun Choi,Suk Yoon Kwon,Jin Seog Kim,Byung Hyun Lee 한국육종학회 2008 한국육종학회 학술발표회 발표요지 Vol.40 No.1