비만 남자의 최대운동시 호흡순환기능 및 혈청지질에 미치는 영향

김귀백,김효은 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

The purpose of this study was to compare and analyze the relation on cardiopulmonary function and serum lipid between 7 normal-weight males and 8 obese males after maximal exercise. 7 normal-weight males were BMI 22.0 and 8 obese males were BMI 25.6. The results of the study were as follows ; 1. Cardiopulmonary Functions All items(VO₂max, VEmax, VO₂max ㎖/㎏/min) of cardiorespiratory function were not significant between two groups after maximal exercise. 2. Serum Lipids All items(TC, HDL-C, LDL-C, TG) of serum lipid were not significant between two groups after maximal exercise. However, Three items(TC, HDL-C, LDL-C) serum lipid were significantly increase in two groups separately(α=.01)

비타민 E와 C의 복합섭취와 비타민 Q섭취가 최대운동시 호흡순환기능 및 Lipoprotein 농도에 미치는 영향

김귀백 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The purpose of this study is to find out the effects of vitamin E,C compound supplement vitamins and vitamin Q supplement on the cardiovescular function and lipoprotein concentration during maximal exercise. The fourteen subjects are highschool students. One group is supplement vitamin E,C compound and another group is supplement vitamin Q during 8weeks, and maximal exercise before vitamin supplements and after 8weeks. The results of this study are as follows: In maximal exercise, cardiovescular fuction is significantly increased, performance also significantly increase, HDL-C indicated significant increase but LDL-C presented reduction, prevent arteriosclerosis.

최대운동시 카페인 섭취가 에너지 기질에 미치는 영향

김귀백 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

The purpose of this study is to analysis the energy substrates of human being of the difference of All-out time between the caffeine-ingested group(CI group) and non-caffeine-ingested group(NCI group). The study also compares the Respiration Function including the difference of Glucose, Triglucerid and Free Fatty Acid at the stage of rest, Max and recovery. The results of the study are as follows; 1. The out-turn of <All-out time> : The CI group is exceeding the NCI group. 2. The out-turn of <Density of Glucose> : The CI group shows lower figure(p<0.01) than the NCI group at the stage of Rest, All-out and after 5, 30, and 60 min. The out-turn of <Density of TG and FFA> : The CI group shows higher than the NCI group. From the above results, the CI group to the human-being's body resulted in no difference All-out time, but the increased Density of TG, and FFA suggests the saving of Glucose. Further, the survey requires some review over the method of selecting the tested students and test method itself.

고강도 운동스트레스가 산화적 손상 및 DNA 손상에 미치는 영향

김귀백 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of the present study was to investigate the effect of exercise stress following exercise intensity on oxidative damage in plasma and DNA damage in lymphocytes and urine. Seven physically healthy male subjects were recruited from I University in Kimhae. In conclusion, our results show that exercise stress (80% HRmax) tended to increase in the oxidative damages of plasma or DNA damage of lymphocytes, Among others, exercise stress following 80% HRmax exercise gave rise to increase oxidative damage in plasma and DNA damage in lymphocytes and urine.

최대운동 강도에 따른 운동스트레스가 DNA 손상에 미치는 영향

김귀백 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

The purpose of the present study was to investigate the effect of exercise stress following exercise intensity on oxidative damage in plasma and DNA damage in lymphocytes and urine. Seven physically healthy male subjects were recruited from I University in Kimhae. They were divided into groups: Maximal intensity. Subjects were tested by work load of treadmill running. In conclusion, our results show that exercise stress (Maximal intensity) tended to increase in the oxidative damages of plasma or DNA damage of lymphocytes. Among others, exercise stress following Maximal intensity exercise gave rise to increase oxidative damage in plasma and DNA damage in lymphocytes and urine.

Treadmill과 Bicycle Ergometer의 운동부하에 따른 육상선수의 심폐능력 및 혈중 Lactate농도 비교연구

김귀백 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.14 No.6

In this study, I selected seven field & track athletes in a certain high school in Changwon, set up the same exercise load of 1Watt/㎏ㆍBW in two exercise workload, Treadmill and Bicycle Ergometer and checked the process of changes in the eight exercise physiological conversions according to equipment and time. The main result is that some athletes with exercise load in Treadmill got higher score than others with Bicycle Ergometer in all eight conversions (HRmin-1, VO₂max, VCO₂max, BFmin-1, RQ, Lactate, etc.) and showed the statistically similar differences. The important result is that VO₂max is 3179±660.04(㎖) in Treadmill and 3075±459.91(㎖) in Bicycle Ergometer; VO₂/㎏ㆍBW is 53.27±10.20(㎖) in Treadmill and 53.97±2.88(㎖) in Bicycle Ergometer; O₂-Pulse is 17.43±3.67(㎖) in Treadmill and 18.54±1.29(㎖) in Bicycle Ergometer during exercise load; RQ is 1.30±0.17 in Treadmill and 1.20±0.21 in Bicycle Ergometer. Lactate, the eighth conversion is 8.14±2.18(mmol) in Treadmill an 6.21±1.77(mmol) in Bicycle Ergometer. In this result, it is shown that it has both strength and weakness to choose the equipments such as Treadmill and Bicycle Ergometer, as a proper exercise load for field and track athletes. Above all, it is the most important to choose which conversion we check first during the exercise load. The results of this study can be used as basic data to choose the proper equipment for each athlete.

Agrobacterium tumefaciens를 이용한 amorpha-4,11-dience 합성 유전자의 상추로의 도입

정귀미,송명종,백남인,박영두 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

Amorpha-4,11-diene synthase(ADS) 유전자를 상추에 도입하기 위해 ADS유전자를 pILTAB357에 삽입하여 binary vector pADS를 제작하였다. 상추 형질전환은 자엽 절편체를 pADS 벡터를 운반하는 A. tumefaciens LBA4404에 접종시키고 MS배지에 200 mg·L 세파탁심과 50 mg·L 가나마이신이 첨가된 선발 재분화배지에서 선발하였다. 선발된 신초는 뿌리 유기 배지에서 뿌리를 유기한 후 뿌리가 발생한 신초는 토양으로 이식하여 재배하였다. 형질전환상추에 T-DNA가 전이되었는지를 확인하기 위해 genomic DNA를 이용하여 PCR과 Southern 혼성화 분석을 실시하였다. PCR 분석 결과 9개의 재분화 개체로부터 기대했던 1.6 kb의 산물을 확인할 수 있었으며 Southern 혼성화 분석 역시 각 재분화 개체에 ADS 유전자가 1개 내지 3개의 copy수로 전이된 것을 확인할 수 있었다. 형질전환 상추내의 artemisinin 함량을 GC와 GC/MS 방법으로 분석하였다. Artemisinin 표준물은 retention time이 10분 7초로 나타났으며 이 peak가 artemisinin인지 확인하기 위하여 GC/MS를 측정한 결과 m/z 282가 정확하게 나타나 artemisinin으로 확인되었다. 한편 형질전환 상추와 일반 대조구 상추의 시료도 GC chromatogram에서 artemisinin의 retention time인 10분 7초 부근에서 여러 peak이 겹쳐서 나타났으나 각 peak에 대하여 GC/MS를 측정한 결과 m/z 282을 나타내는 peak는 관측되지 않아 형질전환 상추와 일반 상추에는 artemisinin이 함유되어 있지 않은 것으로 확인되었다. To introduce amorpha-4,11-diene synthase (ADS) gene into lettuce plant, binary vector pADS was constructed by the insertion of an ADS gene to pILTAB357. Cotyledon explants were infected by A. tumefaciens LBA4404 carrying pADS vector to transform lettuce plant with ADS gene. Transformed shoots were selected on selective regeneration medium containing MS salts, 200 mg·L cefotaxim, and 50 mg·L kanamycin. The selected shoots were transferred to rooting medium, and regenerated shoots were transplanted to pots after roots appeared. PCR and Southern hybridization analysis of genomic DNA were performed to confirm expected T-DNA fragments in the transgenic lettuce plants. PCR analysis revealed the presence of the expected 1.6 kb product from the nine regenerants. Southern hybridization analysis also showed that ADS gene was transferred into transgenic lettuce plants with 1~3 copies. Artemisinin in lettuce was identified by GC and GC/MS analysis. Standard artemisinin was found at 10 min 7 sec in the retention time by GC analysis and confirmed m/z 282 by GC/MS. There were several peaks at retention time of both non-transformed and transformed lettuce plants. However, GC/MS analysis showed that peak with m/z 282 was not found in transgenic lettuce plants.

비타민 E와 C의 복합섭취가 최대운동시 Lipoprotein 농도에 미치는 영향

김귀백,현송자 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study is to find out the effects of compound supplement vitamins E and C on the lipoprotein concentration during maximal exercise. the seven subjects are highschool students and supplement 400IU of vitamin E and 200mg of vitamin C after breakfast during 8weeks, and maximal exercise before vitamin compound supplements and after 8weeks The results of this study are as follows: 1. In maximal exercise before and after taking vitamin E-C compound supplements, oxygen uptake (O₂) and METs significantly (P<.01, P<.05) increased. Ventilation (V_(E)) also increased significantly (P<.05), while heart rate (HR) significantly (P<.05) decreased after vitamin E-C compound supplements. 2. TG was significantly (P<.05, P<.01) lowered before as well as after the maximal exercise. Total cholesterol (T-C) showed significant (P<.05) decrease after the maximal exercise. HDL-C indicated significant (P<.05) increase but LDL-C presented reduction of significance (P<.05) before and after the maximal exercise. The LDL-C/HDL-C ratio significantly (P<.01, P<.05) decreased before and following the training. The T-C/HDL-C ratio also retained significant (P<.05, P<.01) reduction both before and after the running exercise. In conclusion, the compound supplements of vitamins E and C are considered to improve the respiro-circulatory function in exhaustive exercise, decrease an LDL-C concentration, increase an HDL-C concentration and prevent arteriosclerosis.

트라이애슬러의 비타민Q 섭취가 호흡순환기능 및 항산화 효소활성에 미치는 영향

김귀백 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

The purpose of this present study was to investigate the effect of VitaminQ supplementation on antioxidant enzyme activities and cardiorespiratory function following prolonged exercise in trained triathlers. Male 14 healthy college students of trained triathlers participated in exercise sessions. The subjects were divided into 2 groups, as Non-supplement (NS) or VitaminQ supplement(VQ) groups. VitaminQ subjects were participated in two exercise sessions: an initial prolonged exercise(swim 750m, running 10Km) and 70%VO₂max treadmill running exercise. The Non-supplement group fed normal diet. Vitamin Q group administated 90mg/day for 4weeks. Venous blood samples were drown from the forearm antecubital vein pre-supplement, pre-exercise and immediately post-exercise. The results of this study can be summarized as follow: First, the SOD activity of RBC was not changed, while the GPx, GR or GR/GPx activies of Hb were significantly increased following prolonged exercise with CoQ10 supplementation. Second, 70%VO₂max treadmill running exercise increased significantly the VO₂max and V_(E), but the heart rate significantly decreased. In conclusion, the present results clearly show that the Vitamin Q supplementation gave rise to increase the GPx or GR activies and to improve the cardiorespiratory function.

운동전 카페인 섭취가 호흡순환기능에 미치는 영향

김진홍,김귀백 인제대학교 2002 仁濟論叢 Vol.17 No.1

본 연구의 목적은 유아의 놀이 행동 실태를 유형별로 조사하여 분석하여 봄으로써 효율적인 놀이 프로그램을 개발하기 위한 기초자료를 제공하는데 있다. 놀이 행동을 7개 영역으로 나누어 각 영역에 따라 제시한 30개의 놀이 중 10개 정도의 놀이를 중복 선정하게 하여 그 선호도를 성별, 연령별로 분석한 결과 선호율이 높은 놀이는 다음과 같다. 1. 성별에 따른 놀이 선호도 가. 종합놀이 : 모래, 물, 점토, 장난감, 모래쌓기 놀이 등이 높게 나타났으나 남자가 보다 높게 나타났다. 나. 창작놀이 : 지문찍기, 입술찍기, 가면만들기, 상상해서 그림그리기, 동물 흉내내기 놀이 등으로 남녀가 함께 선호하는 놀이로 나타났다. 다. 감각놀이 : 손뼉숫자 맞추기, 맛보기, 교통, 보고따라하기, 색구슬 순서대로 놓기놀이 등이었으나 주로 여자가 많아하는 놀이로 나타났다. 라. 탐구놀이 : 색안경 물감으로 색깔섞기, 정전기, 자석, 올챙이 기르기 놀이 등이었으나 여자가 많이 하는 놀이로 나타났다. 마. 상상놀이 : 유치원, 선생님, 병원 가족, 기차놀이 등으로 여자가 보다 선호하는 놀이로 나타났다. 바. 학습놀이 : 레고 맞추기, 퍼즐맞추기, 끝말잇기, 짝찾기놀이등으로 여자가 선호하는 놀이었다. 사. 대근육놀이 : 과자따먹기, 자전거타기, 볼·풀장, 구르기, 줄넘기 놀이 등이었으나 여자가 더 선호하는 놀이로 나타났다. 2. 연령에 따른 놀이 선호도. 가. 종합놀이 : 5세(비누방울, 물, 모래놀이), 6세(모래, 물, 목공놀이), 7세(모래, 점토, 물놀이) 나. 창작놀이 : 5세(동물흉내내기, 상상해서 그림그리기, 가면만들기놀이), 6세(입술찍기, 지문찍기 놀이), 7세(가면만들기, 지문찍기, 신문 한지그림 그리기 놀이) 다. 감각놀이 : 5세(보고따라하기, 선생님보고 따라하기, 소리듣고 맞추기놀이), 6세(색구슬 순서대로 놓기, 맛보기, 사포로 그림그리기놀이), 7세(손뼉숫자 맞추기, 보고 따라하기, 맛 보기 놀이) 라. 탐구놀이 : 5세(물감으로 색깔 섞기, 금붕어 기르기, 자석놀이), 6세(정전기, 색안경, 자석놀이), 7세(색안경, 물감으로 색깔섞기, 과일 관찰하기 놀이) 마. 상상놀이 : 5세(병원, 유치원, 선생님놀이), 6세(유치원, 선생님, 경찰관 놀이), 7세(유치원, 병원, 선생님 놀이) 바. 학습놀이 : 5세(도형 맞추기, 색깔찾기 놀이), 6세(짝찾기, 빙고, 레고맞추기놀이), 7세(레고 맞추기, 끝말잇기, 받아쓰기 놀이) 사. 대근육 놀이 : 5세(자전거타기, 볼ㆍ풀장, 구르기 놀이), 6세(줄넘기, 과자따먹기, 구르기 놀이), 7세(과자따먹기, 볼ㆍ풀장, 트램프린 놀이) The purpose of this study was to effect on the Cardiorespiratoty functions duning submaximal exercise. According to the administration of dehydeted Caffeine (5mg/kg), 14subject were divided into two groups such as Caffine ingeoted (CI) and non-caffine ingested (NCI) gruop.(Subjects underwent a submaximal exercise of treadmill.) The results of the study are as follows; In the cardiorespiratory functions, heart rate (HR) were diminished by the Caffein Aministration. But VO2 tend to increasd in the Caffeine Ingested. In conclusion, the present results clearly show that the caffeine administration gave rise to an increase fat substrate, but decrease carbodydrate substrate. Funther, the expirmental work test protocol, setecring of subjects and mass of caffeine administration might be necessary to clarify this point.