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Lee, Gil-Yong,Ha, Suk-Jin,Jung, Jong-Hyun,Seo, Dong-Ho,Park, Jong-Yul,Kim, Su-Rin,Park, Nam-Woo,Kweon, Dong-Keon,Park, Sang-Hoo,Park, Cheon-Seok The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3
Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen non-animal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The fmal specific volumetric productivity (0.41 g/L h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.
권오경,성하정,곽형일,방명주,신대희,이진영,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1
Single and 4 weeks oral administration of New Woohwangchungsimwon (NWCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NWCH, 160 ㎎/㎏/day(low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NWCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NWCH in beagle dogs is considered to be safe.
성하정,권오경,방명주,곽형일,이진영,신대희,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1
Single and 4 weeks oral administration of New wonbangwoohwangchungsimwon (NSCH) which was used l-muscone as substitutive material of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs (3 males and 3 females) in acute experiments were administered orally with sinlge dose of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of NSCH, 160 ㎎/㎏/day (low dosage group), 400 ㎎/㎏/day (middle dosage group), 1,000 ㎎/㎏/day (high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korea Food and Drug Administration (1996. 4. 16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ per oral for both male and females. In animals administered with NSCH, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical sign, urinalysis, eye examination, hematology, serum chemistry, organ weight and other fingings. No histological lesions were observed in both control and treatment groups. Above data strongly suggset that NSCH in beagle dogs is considered to be safe.
Ca Ionophore, A23187에 의한 30A-5세포의 지방세포 분화 억제
이권행,윤경하,한만덕 순천향대학교 1993 논문집 Vol.16 No.1
지방세포 분화 억제 과정에서 ??의 역할을 알아보기 위해, 30A-5 preadipocyte가 지방세포로 분화되는 과정에 ?? ionophore, A23187을 첨가하여, 30A-5세포의 지방세포 분화도와 acetyl-CoA carboxylase 활성을 검토하였다. 30A-5 preadipocyte는 insulin과 dexamethasone의 존재하에서 성숙된 지방세포로 분화되는데, 세포가 분화되는 과정에서 1 μM의 ?? ionophore, A23187을 가하면 30A-5 의 지방세포로의 분화가 거의 완전히 억제된다. 30A-5 세포가 지방세포로 분화되는 과정에서 비례적으로 증가하는 지방산 합성의 조절효소인 acetyl CoA carboxylase의 합성은 ?? ionophore, A23187처리에 의해 억제하는데, 30A-5 세포의 지방세포 분화억제는 acetyl-CoA carboxylase합성 저하에 따른 결과로 추측된다. ?? ionophore, A23187에 의한 acetyl-CoA carboxylase 의 합성저하는 비교적 장기간에 걸쳐 나타나는 효과로, 이 억제효과는 ??의 세포막이동의 경쟁적 저해제인 ??에 의해 부분적으로 극복된다. 따라서 A23187에 의한 acetyl-CoA carboxylase의 합성저하와 이에 따른 지방세포로의 분화를 억제하는 과정의 signal tranduction 과정에는 ??의 이동이 중요한 역할을 할 것으로 추정된다. To examine inhibitory effect of ?? ionophore, A23187 on adipocyte conversion of the 30A-5 preadipocytes, we checked the degree of differentiation and acetyl-CoA carboxylase activity of 30A-5 cells in the presence and absence of ?? ionophore, A23187. Inhibition of differentiation of 30A-5 preadipocytes into matured adipocytes was almost completely inhibited by ?? ionophore, A23187. Inhibition of differentiation of 30A-5 cells be resulting from decreased synthesis of acetyl-CoA carboxylase, which is regulatory enzyme in de novo fatty acid synthesis, by ?? ionophore, A23187. Decreased synthesis of acetyl-CoA carboxylase by ?? ionophore, A23187 might be one of the long term effect, and this inhibitory effect was partially recovered by ?? which is competitive inhibitor in membrane transport of ??. Therefore we assumed that ?? mobilization may play a important role on signal tranduction process in inhibitory action of 30A-5 preadipocytes into adipocytes.
비글개에서 l-muscone의 급성독성 및 아급성독성시험 연구
유아선,권오경,성하정,곽형일,방명주,박대규,정규혁,윤효인,조명행 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1
Single and 4 weeks oral administration of l-muscone, a major active ingredient of musk, to beagle dogs of both sexes were performed to investigate both acute and subacute toxicity. Beagle dogs(3 males and 3 females) in acute experiments were administered orally with single dosage of 2,000 ㎎/㎏ and groups of 9 male and 9 female beagle dogs in subacute experiments were given daily different dosage of l-muscone, 0.2㎎/㎏/day(low dosage group), 2 ㎎/㎏/day(middle dosage group), or 20 ㎎/㎏day(high dosage group) once a day for 4 weeks by oral route according to the Established Regulation of Korean Food and Drug Administration(1996.4.16). LD_50 value for beagle dogs was more than 2,000 ㎎/㎏ on oral route for both male and females. In animals administered with l-muscone, there were neither dead animals nor significant changes of body weights. In addition, no differences were found between control and treated groups in clinical signs, urinalysis, eye examination, hematology, serum chemistry, organ weight and other findings. No histolopathological lesions were observed in both control and treatment groups. Above data strongly suggest that l-muscone in beagle dogs is considered to be safe.
Park, Kweon-Ha,Kim, Byung-Hyun The Korean Society of Mechanical Engineers 2000 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.14 No.2
Compression ignition direct injection diesel engines employed a high pressure injection system have been developed as a measure to improve a fuel efficiency and reduce harmful emissions. In order to understand the effects of the pressure variation, many experimental works have been done, however there are many difficulties to get data in engine condition. This work gives numerical results for the high pressure effects on spray characteristics in wide or limited space with near walls. The gas phase is modelled by Eulerian continuum conservation equations of mass, momentum, energy and fuel vapour fraction. The liquid phase is modelled using the discrete droplet model approach in Lagrangian form and the drop behavior on a wall is calculated with a new droplet-wall interaction model based on the experiments observing individual drops. The droplet distributions, vapour fractions and gas flows are shown in various injection pressure cases. In free spray case which the injection spray has no wall impaction, the spray dispersion and vapour fraction increase and drop sizes decrease with increasing injection pressure. The same phenomena appears more clearly in wall impaction cases.