골아세포 MC₃T₃-E₁의 증식과 기능에 Insulin Like Growth Factor Ⅰ, Ⅱ 및 부갑상선 호르몬이 미치는 영향

윤현구,정호연,양인명,김성운,김진우,김광원,김영설,최영길,서광식 대한내과학회 1992 대한내과학회지 Vol.42 No.1

MC₃T₃-E₁골아세포군을 이용하여 IGF-Ⅰ과 Ⅱ를 투여하고 DNA합성과 골아세포기능을 측정하기 위해 PTH에 대한 C-AMP반응정도, alkaline phosphatase 활성도를 측정하여 다음과 같은 결과를 얻었다. 1) IGF-Ⅰ과 Ⅱ는 모두 DNA합성이 어느 농도까지는 증식효과를 나타내지만 그 이후에는 억제되는 양상을 보였다. 2) PTH에 대한 골아세포의 C-AMP반응으로 증가되었지만 IGF-Ⅰ과 Ⅱ의 투여시 농도변화에 따른 의미있는 변화는 없었다. 3) 본 실험의 조건에서 alkaline phosphatase 활성도는 증가되지 않았다. 이상의 결과로 MC₃T₃-E₁ 골아세포에 IGF-Ⅰ과 Ⅱ는 세포의 증식을 자극하여 PTH는 C-AMP생성을 촉진하는 효과가 있었다. The effect of insulin-like growth factor (IGF) Ⅰ and Ⅱ on cloned MC₃T₃-E₁ cells that had osteoblastic activity was examined by [³H] thymidine incorporation into DNA, cyclic AMP responsiveness to parathyroid hormone (PTH), and alkaline phosphatase activity. IGF Ⅰ and Ⅱ stimulated the growth and proliferation of osteoblastic cells at 20ng/㎖ and 50ng/㎖, respectively. We next examined the effect of IGF-Ⅰ and Ⅱ on the expression of osteoblastic activity. Adenylate cyclase response of PTH to IGF-Ⅰ and Ⅱ-treated cells showed maximal stimulation at 10 and 50㎚PTH, respectively, but IGF Ⅰ and Ⅱ induced limited activity of osteoblastic function. In conclusion, IGF-Ⅰ and Ⅱ were potent stimulators to the growth of osteoblastic cells but did not have an immediate effect on expression of osteoblastic function in this experiment.

연습조직과 결과지식의 제공방식이 타이밍 과제 학습에 미치는 영향

윤기운,정광님 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of the present study was to determine generalizability of context interference effect and reduced KR (knowledge of result) relative frequency on the motor learning. Subject characteristics of this study are sixty students of middle school. All subjects were assigned randomly to one of six groups individually. The task of the experiment used a coincident-timing task that can be viewed as a laboratory analogue of a ball-batting task. The Acquisition and retention data of this study were analyzed separately. The trials to criterion data were analyzed using a 2(high-context interference and low- context interference) X 3(summary KR, faded KR, bandwidth KR) X 4(block) ANOVAs with repeated measures on blocks. The post hoc comparisons of means were performed on significant ANOVA effect using the Students Newman Keuls(SNK) test. The results of experiment were as follows ; During acquisition phase, First, the performance of anticipation timing task revealed differences as a function of degree of context interference. Higher the performance of task was found for block context group than for random context group. Two, the performance of task was not found difference in group as a function of reduced KR relative frequency. Three, The interaction effect of context interference and reduced KR relative frequency on the anticipation timing task was marginally significant. That is, Higher the performance of task for block context-faded KR group than for random context-faded KR group, and Higher the performance of task for random context-3 summary KR group than for random context-bandwidth KR group. During retention phase, Four, the performance of anticipation timing task revealed differences as a function of degree of context interference. Higher the performance of task was found for random context group than for block context group. Five, the performance of anticipation timing task was not found difference in group as a function of reduced KR relative frequency. Six, The interaction effect of context interference and reduced KR relative frequency on the anticipation timing task was not significant. In conclusion, the results of the present study suggest that one major of determinant of anticipation timing task is contextual interference condition in practice schedule.

경관 Simulation을 통한 수영강 연접지역의 건축물 고도규제 방안

윤시운,이광국,황영우 東亞大學校 建設技術硏究所 1999 硏究論文集 Vol.23 No.1

This study analyzes the future landscape assesment of Suyoung-River and adjacent areas. Computer Simulation method is used for these purposes. It is expected that the high buildings and high density in Suyoung Information Site might cover the Jang-Mountain view. Therefore this study analyzes the expectations by the experiment of the change of the heights of buildings and the view-point so that Suyoung Information Site can secure the feelings of openness and can be harmonious with natural landscape and skyline. If the high dense buildings are going to be constructed, the optimum condition of development should be between 1.5 and 2.0 of evaluation indicator. The minimum indicator 1.5 would be appropriate as the standard of regulation. The results show that the buildings along the riverside road in the west need to be regulated under the height of 30m(about 10 stories) and the buildings along the Chungryul-Street in the east under the height of 90m(about 30 stories). 하천은 산과 구릉지 등과 함께 도시의 아름다운 경관을 형성하는 중요한 경관요소이다. 도시내 하천변에 나타나는 경관문제는 대부분 대규모 건축물에 의한 경관 훼손에서 비롯된다. 부산의 대부분 하천은 시가지를 통하여 바다로 유입되고 있으나 대부분 복개되었거나 주변지역의 고층건물 입지로 인해 하천 자체가 시민에게 주는 정 (正)의 경관적 요소는 점점 감소하고 있다. 본 연구의 대상지인 수영강은 현재 시가지내를 흐르고 있어 시민에게 주요한 openspace로 기능하고 있다. 주변지역은 지금까지 수영 비행장이라는 기능으로 연해 개발이 유보되어 오다 최근 수영정보단지의 개발계획 등으로 인해 개발압력을 강하게 받고 있다. 향후개발이 진행된다면 수영강변에서 조망할 수있는 주변 자연경관과 시가지 경관은 차폐될 뿐만 아니라, 그 자체의 규모가 크고 형태도 단조로와 위압적이고 획일적인 경관이 형성되게 될 것이다. 현재에도 수영강변의 구릉지에는 아파트 가 입지하여 조망을 차폐하고 있을 뿐 아니라 녹지를 잠식하고 있다. 이러한 현상은 향후 개발압력에 빌려 수영강변의 어느 지역 에서나 발생하는 공통적인 현상이 될 것이다. 이러한 조망차폐, 획일성, 위압감의 조성, 지형의 훼손 및 잠식 등으로 대표되는 수영강의 경관문제는 현행법상 건축심의 시 받는 경관심의로 해결하기에는 한계가 있다. 따라서 본 연구는 수영강과 연접지역, 그리고 배경녹지축인 주변산을 공간적 범위로 하여 첫째, 수영강 연접지역의 범위를 설정하고 연접지역을 몇 개의 경관구역으로 나누어 각 경관구역별 경관의 문제점과 특성을 살펴본다. 둘째, 경관분석을 위하여 Computer를 이용한 수영강변 주요 조망점 에서 사람의 눈에 보이는 대로 주변 경관을 연출해 낼 수 있는 가상의 경관 Simulation을 실시한다. 셋째,이를 바탕으로 수영강 연접지역의 건축물 고도규제 를 위한 기준설정과 고도규제 방안을 제안하는데 목적이 있다.

정상과 갑상선 종양조직에서 사람 IGF-I 유전자의 발현

김성운,장현하,박상미,김덕윤,우정택,양인명,김진우,김영설,김광원,고석환,홍성화,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

Many of the growth-promoting properties of growth hormone(GH) are mediated by insulin-like growth factor-I(IGF-I), a highly conserved circulating 70-amino acid peptide. Recent studies have shown that multiple mechanisms influence IGF-I gene expression, including transcription from two promoters, alternative RNA splicing, and variable polyadenylation. In thyroid tissue, thyroid stimulating hormone(TSH) and IGF-I are the most possible candidates for follicular cell proliferation and hypertrophy. Actually IGF-I had autocrine and paracrine effect for tissue growing. We prepared thyroid tumor tissue mRNAs using single step method for detecting IGF-I levels according to different tissues, i.e., thyroid adenoma or papillary thyroid carcinoma. We used Northern blot analysis for IGF-I mRNA and RNase protection assay (RPA) for IGF-I transcription start sites. For Northern blot, we used whole human IGF-I cDNA as a DNA probe and for RPA, we used IGF-I exon 1 containing noncoding promoter 1 as a riboprobe. We got good RNA bands from Northern blot analysis around 1 kb (IGF-IA) and 7.5 kb (IGF-IB) region. To clarify the amount of both IGF-IA and IB mRNAs, we measured autoradiographied signal of IGF-I mRNAs bands using densitometer. In IGF-IA signals, there's no change among liver and thyroid tissues, but in case of IGF-IB mRNA bands, the signal was markedly increased in thyroid carcinoma tissues than that of normal thyroid tissue (85% vs 14%). In the study of RPA, all thyroid tissues used the same transcription start sites as those of liver's. We concluded that that this different regulation of IGF-I mRNA was originated from tissue specificity. That meant some tissue specific transcription factor/s were related to tissue IGF-I expression.

부산시 중심지체계 설정에 관한 연구

임채성,이광국,윤상복,윤시운 동아대학교 건설기술연구소 2000 硏究報告 Vol.24 No.2

This study was aimed to establish the system of centers and to offer the viewpoint to studies and policies of urban spatial structure while analyzing their characteristics in Busan. The results of this study were summarized as followed : According to the result about establishment of the system of centers, 2 Centers, 4 Sub-Centers and 5 low-lanking centers were identified. This is a little different with 2 Centers, 6 Sub-Centers, 2 District-Centers written in 「Busan Master Plan toward 2011's」. In other words, the system of centers in Busan was represented more subdevided and abundant than that of 「Busan Master Plan toward 2011's」 and these centers had both polycentric and multi-nuclei characteristics. With premise that the urban structure is being changed on the basis of the change of a period, society and economy in the future to establish effective system of centers, it is advisable that the establishment of the system of centers is to readjust by stage in the process of planning.

부산시 중심지체계 설정에 관한 연구

임채성,이광국,윤상복,윤시운 東亞大學校 建設技術硏究所 2000 硏究論文集 Vol.24 No.2

This study was aimed to establish the system of centers and to offer the viewpoint to studies and policies of urban spatial structure while analyzing their characteristics in Busan. The results of this study were summarized as followed : According to the result about the establishment of the system of centers, 2 Centers, 4 Sub-Centers and 5 low-lanking centers were identified. This is a little different with 2 Centers, 6 Sub-Centers, 2 District-Centers written in 「Busan Master Plan toward 2011's」. In other words, the system of centers in Busan was represented more subdivided and abundant than that of 「Busan Master Plan toward 2011's」 and these centers had both polycentric and multi-nuclei characteristics. With premise that the urban structure is being changed on the basis of the change of a period, society and an economy in the future to establish effective system of centers, it is advisable that the establishment of the system of centers is to readjust by stage in the process of planning.

장성호 수자원 이용 패턴

윤광식 ( Yoon Kwang-sik ),한국헌 ( Han Kuk-heon ),윤석군 ( Yoon Suk-gun ),정재운 ( Jung Jae-woon ) 한국농공학회 2003 한국농공학회 학술대회초록집 Vol.2003 No.-

The Water resources utilization pattern of Jangsung reservoir was studied. The observed precipitation and existing reservoir operation data such as irrigation amount, reservoir storage, river maintenance requirement, flood control discharge were collected for ten years period and analyzed. Major findings of this study are as follows: The observed average, minimum, maximum annual precipitation were 905.1mm, 1,977.3mm, 1,554.3mm during study period, respectively. The average annual irrigation amount was 554.5mm, irrigation amount of drought years of '92 and '94 was 604.6mm, 679.2mm, respectively. However, irrigation amount of extended drought year '95 was 384.9mm. It showed that supplying capacity of Jangsung reservoir was limited when consecutive 2 year drought occurred. The main water resources usage of Jangsung reservoir was irrigation, but flood control discharge exceed irrigation amount exceptionally when high precipitation occurred. The reservoir operation record revealed that discharge for river maintenance was delivered even drought years.

포항 인공저류층 지열시스템 프로젝트에의 미소진동 관리방안 제시

김광일(Kwang-Il Kim),김광염(Kwang-Yeom Kim),민기복(Ki-Bok Min),최재원(Jae-Won Choi),윤운상(Woon Sang Yoon),윤건신(Kern-Shin Yoon),윤병준(Byungjoon Yoon),송윤호(Yoonho Song),이태종(Tae Jong Lee) 한국신재생에너지학회 2016 한국신재생에너지학회 학술대회논문집 Vol.2016 No.11

Surface Imaging of Barley Aleurone Cell by Atomic Force Microscopy

Kwang-Woon Huh,Seung-Hwan Kim,Hyun-Hwoi Ku,Byung-Moo Lee,Jae Yoon Kim,Yong-Won Seo,Tae Wan Kim 韓國作物學會 2004 한국작물학회지 Vol.49 No.1

To observe and analysis ultra-microscopically barley aleurone cell surface, atomic force microscope (AFM) was used. Seed coat of early maturing germplasm, eam9, was dehulled and scanned by non-contact mode. We have obtained the high resolution topographic 3-dimensional image of barley aleurone layer with high resolution. These images showed the membrane proteins in barley aleurone cell. One channel protein and numerous peripheral or integral proteins were detected in a area of 100 ~mu~textrmm2 . Furthermore, we found that their widths were ranged from 50 to 750nm and lengths from 0 to 66 ~mu~textrmm . The thickness of aleurone layer was measured by scanning electron microscope. The thickness at early developmental stage was about 16 and then the aleurone cell enlarged upto 57 ~mu~textrmm ~mu~textrmm at least until 42 days after anthesis. In this study, we firstly reported on the ultrastructural AFM analysis of living aleurone cell as a biological specimen. It was clearly suggested that AFM will become an powerful tool for probing both the structural properties of biological samples

연구원 활동 : 발표논문요약 ; < 전기공학분야 >

(Kwang Woon Lee),(Ji Yoon Yoo),(Gwi Tae Park) 고려대학교 공학기술연구소 1996 생산기술연구소보 Vol.3 No.-