운동강도의 차이가 대장에서의 Heme oxygenase-1 발현에 미치는 영향: Oligonucleotide chip microarray analysis

최은주 ( Eun Ju Choi ),류호영 ( Ho Young Ryu ),차광석 ( Kwang Suk Cha ) 한국운동생리학회(구-한국운동과학회) 2012 운동과학 Vol.21 No.1

이 연구의 목적은 운동강도 차이에 의한 대장조직이 Heme oxygenase-1(HO-1)의 mRNA 발현에 어떠한 영향을 미치는지 규명하기 위해, Sprage-Dawley계 흰쥐를 대상으로 통제그룹(CON)과 저강도 운동그룹(LIE), 고강도 운동그룹(HIE)으로 구분하여 4주 동안 트레드밀운동 후 48시간 이후에 High through-put microarray analysis방법으로 다음과 같은 실험을 실시하였다. 본 실험은 Rat ABI oligo chip 26,857 개의 유전자 중 filtering을 통하여 12.079개 유전지를 선택하였고, 이 중 유의성 있는(p<.05) 유전자 12,072개를 선별하였으며, clustering분석 방법과 면역조직화학염색법에서도 HO-l이 운동강도에 따라 유의하게 발현하였다(p<.05). Microarray분석 후 RT-PCR로 확인한 결과 HO-I의 유전자 발현양상이 일치하는 결과를 얻었다. 이 실험의 결과로서 운동이 대장에서 HO-1 발현에 영향을 주는 것을 알 수 있었다. 운동이 대장에서의 mRNA 발현이 저강도 운동그룹(LIE)보다 고강도 운동그룹(HIE)에서 더 많이 발현되어지는 것으로 보아 고강도 운동에 의해 발생되는 산소라디칼을 HO-l의 유도를 통하여 적절히 제거함으로써 스스로 항상성을 유지하고 있는 것으로 생각되어진다. The purpose of this study is to identify how large intestine tissue effects to mRNA expression in Heme oxygenase-l (HO-1) due to differences in exercise intensity. To perform this experiment we divided Sprage-Dawley rats into 3 groups (control group (CON). low-intensity exercise group (LIE) and high-intensity exercise group (HIE)), and in 48 hours after 4 weeks treadmill exercise. the following experiment was performed with "High through-put micro array analysis methods" . 12,079 genes were chosen by filtering of the Rat ASI oligo chip 26.857 genes in this experiment Among 12.079 genes, we selected significant genes (p<.05) that were also expressed in the ways of clustering analysis and immunohistochemistry. With results of RT-PCR confirming and microarray analyzing, we derived that gene expression profiles of HO-l had been consistent. As a result of this experiment, we certainly identified that exercise effects to HQ-l expression in large intestine. mRNA expression was more expressed in high-intensity exercise group CHIE) than low-intensity exercise group (LIE), it seems that homeostasis maintains itself by properly removing oxygen radical. caused by high intensity exercise. through reduction of HO-l.

국산 Lidocaine 알칼리화를 위한 Bicarbonate 혼합시 침전유발 최소량에 대한 고찰

이선호,김현수,곽인숙,안원식,최관호,김광민,손민제 대한마취과학회 2000 Korean Journal of Anesthesiology Vol.39 No.5

Background : When local anesthetics for regional anesthesia is used, usually small amounts of bicar-bonate are added for rapid onset. This addition gives the mixed solution a more alkaline pH. The following result is an increased unionized form of the local anesthetic and rapid penetration of the drug into tiissue. Unfortunately, no data about adequate mixing volumes of domestic lidocaine and bicarbonate is available. Methods : We examined six mixing pairs of two kinds of 2% lidocaines and three kinds of 8.4% bicarbonates for minimum volumes of bicarbonate to cause a precipitation of 2% 20 ml lidocaine. Results : The mean volumes of bicarbonate to cause precipitation were 1.54 ml for Kwang-Myung lidocaine with Kwang-Myung bicarbonate, 2.90 ml for Kwang-Myung lidocaine with Dae-Won bicarbonate, 2.73 ml for Kwang-Myung lidocaine with Je-Il bicarbonate, 0.97 ml for Je-Il lidocaine with Kwang-Myung bicarbonate, 1.26ml for Je-Il lidocaine with Dae-Won bicarbonate and 1.39 ml for Je-Il lidocaine with Je-Il bicarbonate. Conclusions : We conclude that the Kwang-Myung lidocaine and the Je-Il lidocaine could cause precipitation when mixing with a smaller bicarbonate volume than foreign textbook recommended. (Korean J Anesthesiol 2000; 39: 726-729)

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 東亞大學校 建設技術硏究所 1997 硏究論文集 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Arch교의 橫桁連結部 局部應力과 疲勞擧動에 관한 硏究

최광순,조호근,손영호,최광규 동아대학교 건설기술연구소 1997 硏究報告 Vol.21 No.2

In this paper, the relation between the local stress and the fatigue behavior at gusset plate connected with main girder and floor beam in arch bridges was obtained. The conclusion was as follows : (1) In-plane bending moment and out-of-plane bending moment are higher than other internal stress resultants. (2) When a vehicle crosses the bridges, the change of out-of-plane bending moment corresponds with the change of relative difference in displacement in the longitudinal direction between the tie-girder and stringer. (3) The change of in-plane bending moment corresponds with the change of the displacement in the transverse direction of arch-rib. (4) Fatigue crack can be initiated by in-plane bending stress that Is produced by out-of-plane bending moment at the toe of gusset plate. (5) Fatigue crack can be initiated by tension stress that is produced by in-plane bending moment at the toe of gusset plate.

Azithromycin으로 치료한 임신 중 쓰쓰가무시병 1예

김광석,최진욱,서호종,김기훈,박성호,서광섭,고성만,김순혜,김호정 대한감염학회 2001 감염 Vol.33 No.5

We report a case of tsutsugamushi disease in a 26 year-old pregnant woman who was treated with azithromycin. Her gestation period was 27 weeks and she admitted with fever, rash, and eschar on the right shoulder. Currently recommended medications for the treatment of scrub typhus are doxycycline or chloramphenicol. But, these drugs are class D drugs according to the U.S. Food and Drug Administration (FDA) Fetal Risk Summary, so they couldn't be used to treat pregnant women. Recently, a few case reports suggested that azithromycin, a relatively new macrolide antibiotic, was effective and safe for the treatment of scrub typhus in pregnant women. And, there is no evidence that azithromycin causes harmful effects to the developing fetus or to children. On the basis of current in vivo test that confirms the effectiveness of azithromycin, it may be the drug of choice for the treatment of scrub typhus in pregnant women and children. (Korean J Infect Dis 33:380-382, 2001)

원자력 사고후 쌀알과 배추내 ^90Sr 및 ^137Cs 농도 예측

최용호,임광묵,황원태,이한수,이창우 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.3

원자력 사고후 시간경과에 따른 작물체내 핵종 농도를 보다 현실적으로 예측할 수 있는 방법을 수립하고 단위 건침적에 대하여 백미와 배추내 ^90Sr 및 ^137Cs의 50 년간 농도를 예측하였다. 비생육기 사고의 경우 농도는 두 핵종 모두 시간경과에 따라 서서히 감소하였고 50년 내내 ^90Sr이 ^137Cs보다 높았다. 생육기 사고의 경우 처음 1년간 농도는 비생육기 사고에 비해 대체로 ^90Sr은 최고 30 배, ^137Cs은 최고 1,000 배 정도까지 높았다. 50 년간의 누적농도는 백미에서는 비 생육기 사고의 경우 ^90Sr이, 생육기 사고의 경우 ^137Cs이 더 높았으나 배추에서는 어느 경우에나 ^90Sr이 더 높았다. 생육기 사고시 50년간의 누적농도에 대한 지배적 경로는 대체로 ^90Sr의 경우 뿌리흡수, ^137Cs의 경우 작물체 직접오염이었다. 재부유의 영향은 무시할 수 있을 정도였다. 예측 결과에 입각하여 사고 조건별로 대책의 방향을 제시하였다. A method of more realistically predicting radionuclide concentrations in crop plants varying with time after a nuclear accident was established to estimate 50 years' concentrations of ^90Sr and ^137Cs in polished rice seeds and Chinese cabbage for unit dry deposition. After non-growing season accidents, concentrations of both nuclides decreased gradually with time and ^90Sr concentrations were higher than those of ^137Cs throughout the whole period. Radionuclide concentrations in the 1st year after growing season accidents were on the whole higher than those after non-growing season accidents by factors of up to 30 for ^90Sr and up to 1,000 for ^137Cs. In polished rice seeds, the 50 years-integrated concentration was higher for ^90Sr than for ^137Cs after non-growing season accidents, whereas the opposite was true after growing season accidents. In Chinese cabbage, however, it was higher for ^90Sr than for ^137Cs after both types of the accident. Generally speaking, the dominant pathway for the integrated concentration after the growing season accident was root uptake for ^90Sr and direct plant contamination for ^137Cs. The effect of resuspension was negligible. Based on the predicted results, the direction of planning countermeasures was suggested for various accident conditions.

다양한 함불소가스들의 고비점 용매에 관한 용해도 측정 및 엑막에 의한 분리

최평호,이상학,김철웅,김범식,김광주,이정민,박인준 한국공업화학회 2002 응용화학 Vol.6 No.2

The solubility of various fluoro-gases in high boiling-point solvents, n-Cnh_2n+_1OH(6<n<10)and ionic liquids(+ ions: 1-Buty 1-3-methy1-imidazolium, 1-Ethy1-3-methy1-imidazolium, - ions:BF_4, PF_6, S_6F_6, F_6O_4S_6) were measured at temperatures from 0 to 30℃, at total pressures up to 4 bat Solubility increased significantly with the decrease of n in n-Cn_2N+OH and showed no such a difference in the types of ionic liquids, Especially, the solubility of R22 gas in these solvents increases rapidly with increases in pressure and decreases in temperature, whereas other fluoro-gases were showed a little solubility Liquid-supported membranes were prepared with these solvents and polymer matrix and were applied to the separation of fluoro-gases including R22 gas. A high permeability and selectivity were exhibited in R22 gas against other fluoro-gases.

ZnS 완충층을 사용한 SrS:Ce, Cl 박막 EL소자의 발광특성

최광호 남서울대학교 2005 남서울대학교 논문집 Vol.11 No.2

Luminescence characteristics of SrS:Ce,Cl TFEL devices with ZnS buffer and double insulating layers has been investigated. The density of transferred charge is gradually increased along with the Applied voltage up to 200V and that it is sharply increased along with the applied voltage above 200V. This phenomenon is thought to that the density within shallow trap is relatively low and that it within deep trap is high and distributed wide. The crystallinity of a SrS:Ce,Cl active layer is strongly affected by the underlayer. Bright blue emission is achieved in a thin film EL device having a strongly (111) oriented ZnS film as its underlayer.

지반-구조물 상호작용에서의 JOINT 요소에 대한 연구

최광순,손영호,김종주,최광규 동아대학교 건설기술연구소 1999 硏究報告 Vol.22 No.2

The direct boundary element method has been formulated In which the symmetry condition is introduced. The displacement approach of the finite element method has been reviewed where the joint stiffness formulation is included. The coupling of boundary and finite element methods is derived for both equivalent boundary and finite element approaches. A computer program based on the coupling of boundary and finite element methods was developed to analyze the elastic behavior of a structure. Another computer program based on the finite element method alone was developed to solve the same soil mass which were assumed to be linearly elastic, isotropic, and homogeneous. The characteristics of joint elements are that they will separate in response to tension, slide in response to shear, and transmit any force in response to compression. The limitation of no transfer of tensile stresses across soil/structure interface was ensured by employing iterative schemes on joint elements. The iterative solution to simulate real properties of joint elements is based on the so called "load transfer method" An example problem of soil-structure interaction problem has been analyzed using the two numerical methods indicated above. Comparison of the results of nodal displacements obtained from both techniques and the software CRISP demonstrates the accuracy and validity of the coupling of boundary and finite element methods. Due to the fact that the dimensionality of the boundary element region is reduced, the equations generated by the coupling of boundary and finite element methods are fewer than the equations generated by the finite element method alone in acquiring the same acruracy. Therefore, the coupling of boundary and finite element methods is more efficient than the finite element method alone in solving a complex soil-structure interaction problem.

압자의 압입에 의한 치관 전장용 복합레진의 기계적 성질 평가

최원호,배태성,박광선,박종수 대한치과기재학회 2001 대한치과재료학회지 Vol.28 No.2

This study was performed to evaluate the mechanical properties of four commercially available veneering resin composites for crown (Dentacolor: DC, Artglass: AG, Esternia: ET and Targis: TG). According to the manufacturer's instructions, disc specimens were fabricated in a teflon mold giving 12mm in diameter and 1 mm in thickness. All specimens were immersed in distilled water at 37℃ for 30 days. An indentation test of 68-degree trigonal diamond pyramid was made under 98.06 mN load for 15 seconds. Hardness numbers, charateristic indentation depths, flow and permanent deformation were measured and compared statistically by ANOVA and Tukey's multiple range test. The results obtained were summarized as follows; 1.The highest hardness value of 87.4 was observed in the ET group and the lowest value of 11.8 was observed in the DC group. Hadness values showed no sinificant difference between the AG group and TG group, but others showed the significant difference beween groups (p<0.5). 2.Flow value of the ET group was significantly lower than those of the AG and TG groups of hybrid composite for crowns. 3.Percent strain during the indentation was the lowest in the DC group and the highest in the AG group, but there was no significant difference in each experimental group (p>0.05). 4.The relationship between the hardness number and flow during the indentation was a negative linear on a logarithmic scale.