Diazepam의 腸管運動 抑制機轉에 關한 硏究

鄭址昌,高啓昌 慶熙大學校 1985 論文集 Vol.14 No.-

In order to investigate the mechanism of depression of intestinal motility with diazepam, the influences of metabolic substrates on the motility of isolated rabbit doudenum depressed with diazepam were observed. The following results were obtained; 1. motility of isolated duodenum was depressed about 40% with 0.3mM diazepam, as compared with the control. 3. Pyruvate, at concentration of 10nM, remarkably restored the motility of the duodenum depressed with diazepam in normal tyrode medium or glucose-free medium. 4. Acetate, at concentration of 10mM, also produced significant recovery of the motility of the diazepam-depressed duodenum in normal tyrode medium of blucose-free medium. 5. Fructose, at concentration of 30mM, showed little effect on the motility of the diazepam-depressed duodenum in normal tyrode medium or glucos-free medium. 6. The motility of the diazepam-depressed duodenum in the normal tyrode medium or glucose-free medium was little regained with glucose at concentration of 30mM 7. Pyruvate (10mM), acetate (10mM), fructose (30mM) and glucose (30mM) had no significant effects on the motility of the isolated control duodenum. It is concluded from the results that the glycolysis in the intestinal smooth muscle might be inhibited with diazepam and then the inhibited glycolysis might have depressed the motility of isolated rabbit-duodenum.

Cefotaxime과 Furosemide의 賢排泄 및 血漿蛋白 結合에 미치는 影響

鄭址昌,金載學,韓大燮,高啓昌 慶熙大學校 1984 論文集 Vol.13 No.-

The cephalosporins are eliminated by both glomerular filtration and tubular excretion. Chronic administration of cephalosporins may cause renal tubular necrosis. Cefotaxime is a new semisynthetic cephalosporin antibiotic with an unusually broad antibacterial spectrum. Therefore, to determine whether any variations at plasma protein binding with furosemide by cefotaxime were responsible for its diuretic action of urine volume and electrolytes in rabbits, author studies effects on drug-interaction of cefotaxime and furosemide. The following results were obtained. 1. The group, administrated simultaneously furosemide and cefotaxime, was significantly increased compared with furosemide alone group on the urine volume, urinary electrolytes, because markedly increased the inhibition of Na^+ and Cl^- reabsorption in the renal tubule, and glomerular filtration rate without any differences. 2. Serum concentration of cefotaxime in rabbits was significanly elevated by the combination with furosemide, and markedly inhibited the elimination levels of cefotaxime in urine of rabbits. Conclusion of above results; Synergistic effect of cefotaxime on diuretic action of furosemide showed the comparative inhibition of plasma protein binding with furosemide by cefotaxime, and the continuation of diuretic effect seems excretion of furosemide on the in-hibited by competition between furosemide and cefotaxime in renal tubular excretion.

Ampicillin과 Furosemide의 相互作用에 관한 硏究

李昇宰,鄭址昌,高啓昌,韓大燮 慶熙大學校 1986 論文集 Vol.15 No.-

In order to investigate the durg-interaction between furosemide and ampicillin, the influences of ampicillin on the diuretic action of furosemide and the effects of furosemide on the urinary elimination rate and serum concentration of ampicillin were studied in the rabbits. In comparison of diuretic effects of furosemide alone group with those of furosemide-ampicillin combined group, the furosemide-ampicillin combined group showed significant increase in urine flow and urinary electrolytes amounts. Those findings seem to be resulted chiefly from marked inhibition of sodium and chloride reabsorption in the renal tubule without a little alteration of glomerular filtration rate. Throughout the combined administration of furosemide and ampicillin. the serum concentration of ampicillin were significantly elevated, but the urinary elimination rate of ampicillin were markedly decreased in the observed rabbits. It is concluded from the results that synergistic effect of ampicillin on diuretic action of furosemide might be due to the competitive inhibition of plasma protein binding with furosemide by ampicillin. It is also postulated that the competition between furosemide and ampicillin in renal tubular secretion.

Furosemide와 Cephapirin의 相互作用에 관한 硏究

李淇範,鄭址昌,高啓昌,鄭柱浩 慶熙大學校 1988 論文集 Vol.17 No.-

Cephapirin is a semisynthetic cephalosporin antibiotic with an unusually broad antibacterial spectrum, which is stable in beta-lactamase and is poorly absorbed from the gastro-intestinal tract and is given by intramuscular of intravenous injection as the sodium salt. High concentrations of furosemide and cephapirin are excreted in the urine by proximal tubular secretion. In order to investigate the drug-interaction between furosemide and cephapirin, the influences of cephapirin on the diuretic action of furosemide were studied in the rabbits. In comparison of diuretic effects of furosemide alone group with those of furosemide-cephapirin combined group, the furosemide-cephapirin combined group showed significant increase in urine flow and urinary electrolytes amounts. These findings seem to be resulted chiefly from marked inhibition of sodium and chloride reabsorption in the renal tubule without a little alteration of glomerular filtration rate. It is concluded from the result that synergistic effect of cephapirin on diuretic action of furosemide might be due to the competitive inhibition of plasma protein binding with furosemide by cephapirin. It is also postulated that the cephapirin-elimination through the renal tubule may de delayed by the competition between furosemide and cephapirin in renal tubular secretion.

The Effect of Fructose on the Force of Contraction of Procaine-depressed Isolated Hearts

Ko, Kye-Chang 慶熙大學校 1987 論文集 Vol.16 No.-

외과영역에서 널리 쓰여지고 있은 국소 마취제인 procaine의 심근 수축력 억제작용에 관한 기전을 규명하기 위하여, Procaine에 의해 수축력이 억제된 흰쥐 적출심장에 대한 외인성 대사기질인 fructose의 효과를 관찰한 바 다음가 같은 실험 평가를 얻었다. 1. 기질제거에 의해 감소된 적출심방의 수축력을 fructose는 농도에 비례하여 증가시켰으며, 30mM에서 최대 효과를 나타내었다. 2. Krebs-Ringer glucose 용액에 현수한 적출심방의 수축력은 0.3mM Procaine에 의해 약 50%의 감소를 나타내었으며, 30mM fructose의 투여는 이 감소된 적출심방의 수축력을 현저히 증가시켰다. 이 결과는 Procaine에 의해 저하된 적출심방의 수축력 증가에 fructose가 energy원으로 쓰여지고 있음을 시사하고 있다. 3. 심근의 수축성에 대한 fructose의 영향을 다른 실험조건하에서 검토하기 위하여, 저 Calcium(1/2) 농도의 Krebs-Ringer glucose medium이 사용되었으며, 이 저 Calcium(1/2) medium에 현수된 적출심방의 수축력은 약 50%로 감소되었다. 또한 fructose(30mM)는 이 저Calcium(1/2) medium에서 감소된 적출심방의 수축력을 증가시키지 못하였다. 4. Fructose는 정상적인 적출심방의 수축성에 대해 별 영향을 나타내지 못했다. 그러나 glucose는 정상심방의 수축력을 농도에 비례하여 증가시켰다. 5. Fructose의 적출심방에 대한 수축력의 증가능력은, 기질제거용액에 의해 감소된 심방의 수축력에 대한 증가율이 Procaine에 의해 감소된 심방의 수축력 증가율보다 현저히 높게 나타났다. 이상의 실험성적에서 Procaine은 심근 세포내에서 포도당 대사를 해당과정에서 억제하고 있음을 시사하고 있으며, 나아가서 Procaine에 의한 심근세포내의 대사억제는 해당과정에서 Phosphofructokinase step 이전의 초기단계에서 억제되고 있음을 시사하고 있다.

Metabolizable Substrates on Functional Activity of Isolated Human Myocardium

KO, Kye-Chang 慶熙大學校 1974 論文集 Vol.8 No.-

心臟의 搏動에 必要한 힘은 環境條件에 따라 포도糖을 비롯한 各種代謝基質을 燃料로 하여 生産된다는 事實은 過去 많은 動物 및 臨床實驗에 依하여 硏究 確認되어 왔다. 그러나 사람의 遊離心臟에 依한 代謝基質의 直接效力에 對한 報告는 文獻上 이를 찾아볼수 없었다. 本硏究者는 美 Indiana大 醫科大學 心臟外科의 協力下에 心臟手術時 摘出되는 사람 心臟右心房의 一部를 使用, 그의 體外搏動實驗에 成功, 遊離人 心臟의 收縮性에 對한 各種 代謝基質의 直接效果를 比較 檢索立證하였다.

STUDIES ON THE EFFECT OF PYRUVATE ON HALOTHANE-DEPRESSED RAT ATRIA

Ko, Kye Chang 慶熙大學校 1972 論文集 Vol.7 No.-

Mechanism of Depressant Action of Inhalation Anesthetics on Heart : 2. Contractile Activity of Halothane-Depressed Isolated Myocardium by Various Substrates 第2報 Halothane에 限하여 抑制된 心臟의 收縮性에 미치는 各種基質의 效果

KO, Kye Chang,AHAN, Chi Yul 慶熙大學校 1974 論文集 Vol.8 No.-

모든 吸入全體麻醉劑는 心臟機能을 抑制한다는 事實은 이미 알려진바 있으며, 筆者는 그 機序의 究明을 心臟代謝學的 側面에서 관찰하기 위하여 全麻劑 halothane에 依하여 抑制된 遊離心房의 收縮性에 對한 各種 代謝基質의 效果를 檢討하였다. pyruvate, lactate, acetate 等의 代謝基質은 halothane―低下의 心收縮性을 增加시켰으나, glucose는 濃度의 如何를 莫論하고 效力이 없었다. 本實驗은 筆者가 第1報에서 halothane의 心臟抑制原因은 心臟代謝의 抑制 特히 解糖作用의 初期段階의 抑制에 있다고 發表한 假說을 立證하는 것으로 結論 질수 있다.

Cardiac Depressant Action Mechanism of Intravenous Anesthe tics

Ko, Kye Chang 慶熙大學校 醫科大學 1981 慶熙 醫大 論文集 Vol.6 No.1

Experimental Studies on Substrate Utilization of Isolated Atria Depressed by Acidosis

Ko, Kye Chang,Jung, Jee Chang,Han, Dae Sup 慶熙大學校 醫科大學 1979 慶熙 醫大 論文集 Vol.4 No.1