Localization of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal Antibody

Kang-seuk Choi,Jin-ju Nah,Young-joon Ko,Shien-young Kang,Yi-seol Joo 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.2

of Antigenic Sites at the Amino-terminus of Rinderpest Virus N Protein Using Deleted N Mutants and Monoclonal AntibodyKang-seuk Choi*, Jin-ju Nah, Young-joon Ko, Shien-young Kang1 and Yi-seok JooNational Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea1Research Institute of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University, 48 Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk 361-763, KoreaReceived April 2, 2003 / Accept July 10, 2003J. Vet. Sci. (2003), 4(2), 167-173JOURNAL OFVeterinaryScience*Corresponding author: Kang-seuk Choi National Veterinary Research and Quarantine service, Ministry of Agriculture and Forestry, 480 Anyang, Gyounggi 430-824, Korea Tel: +82-31-467-1860, Fax: +82-31-449-5882 E-mail: choiks@nvrqs.go.kr

Nuclear Factor I-C 결손 생쥐에서 상아모세포의 형태학적 특징

고승백,이창섭,이난영,이상호,김흥중,박주철 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

NFI-C K/O 생쥐에서는 상아모세포의 분화과정에 이상이 초래되어 상아질 형성에 이상이 생기고 치근 형성이 불완전하게 이루어지는 것으로 알려져 있으나 이에 대한 명확한 기전은 잘 알려져 있지 않다. 상아모세포가 분화하여 정상적으로 상아질을 형성하기 위해서 핵과 세포질이 극성을 띠고 잘 조직화되어야 하며, 이 과정에서 다양한 세포사이 결합장치들이 중요한 역할을 하는 것으로 알려져 있다. 본 연구에서는 NFI-C K/O 생쥐에서 상아질 형성에 이상이 생기는 것이 상아포세포의 형태학적 변화와 세포사이 결합장치들이 기능을 하지 못한 결과에서 기인한 것인지 알아보기 위하여, NFI-C K/O 생쥐에서 발생한 비정상적인 상아모세포들을 광학 및 투과 전자현미경을 이용하여 형태학적으로 관찰하고, Zo-1과 occludin의 발현을 면역조직화학적으로 관찰하여 세포사이 결합장치들의 분포를 확인하여 다음과 같은 결과를 얻었다. 1. 광학현미경 소견에서 NFI-C K/O 생쥐의 전치부 상아모세포는 세포 극성이 상실되고, 여러 층으로 배열되어 있었으며 상아질에 많은 세포들이 함입된 것과 같은 비정상적인 상아질의 소견을 나타냈다. 반면에 NFI-C K/O 생쥐의 구치부 상아모세포는 치관부에서는 잘 조직화된 소견을 보였으나 치근 형성 부위에서는 세포 배열이 불규칙해지고 세포 극성이 상실되었다. 2. 투과 전자현미경 소견에서 NFI-C K/O 생쥐 전치부의 비정상적인 상아모세포는 둥근 형태로 세포 사이 간격이 넓으며 폐쇄연접과 같은 세포사이 결합장치들이 전혀 관찰되지 않았다. 3. Zo-1의 면역조직화학적 염색에서 NFI-C K/O 생쥐의 전치부 법랑모세포의 근위부와 원위부에서 ZO-1이 강하게 발현되었으나 비정상적인 상아모세포에서는 ZO-1의 발현을 관찰할 수 없었다. 4. Occludin의 면역조직화학적 염색에서 정상 생쥐의 전치부 상아모세포에서는 occludin의 발현이 관찰되었으나 NFI-C K/O 생쥐의 비정상적인 상아모세포에서는 occludin의 발현이 관찰되지 않았다. 이상의 결과를 종합하여 볼 때 NFI-C의 결손은 상아모세포의 분화 이상을 초래하고 비정상적으로 상아질을 형성하는 과정에 세포사이 결합장치의 상실과 같은 형태학적인 변화의 중요한 요소로 작용하는 것으로 생각된다. NFO-C null mice demonstrated aberrant odntoblast differentiation and thus abnormal dentin formation while other tissues/organs in the body, including ameloblasts, appear to be unaffected and normal. However, little is known about the mechanism of NFI-C function in odontoblast differentiation and dentin formation. Odontoblasts are tall, highly polarized cells that are responsible for formation and maintenance of the predentin and dentin. An indication of their polarity is the acquisition of specialized intercellular junctions. As predontoblasts differentiate into odontoblasts, they are joined and attached at the apical end by well developed terminal webs of cytoskeletal actins, and associated tight as well as adherent njunctions. In this study, in order to investigate if disruption of the NFI-C gene interferes with formation of a specific or other structural proteins of the intercellular junctions, we examined morphological charcteristic of the aberrant odontoblast in NFI-C null mice using light and electron microscope. In addition, we determined the expression of major structural proteins of intercellular junctions, ZO-1 and occludin, during the differentiation of odontoblasts using immunohitochemistry. The results were as follows: 1. In light microscopy, abnormal odontoblasts of incisors of the NFI-C null mice were round in shape, lost their polarity, and trapped in osteodentin-like mineralized tissue. Mutant molars have relatively normal crowns, but short and abnormal differentiating adontoblasts in root formation area. 2. Electron microscopy of abnormal odontoblasts revealed the dissociation of the round osteoblast-like cells, the loss of their cellular polarity, and the absence of an intercellular junctional complex known as the tight junctions. 3. A mutant incisor showed labeling for ZO-1 at the proximal and distal ends of secreting ameloblasts, while staining for ZO-1 was not observed in the abnormal odontoblasts. 4. A normal incisor showed immunoreactivity for occludin in the differentiating odontoblasts. However, staining for occludin was not observed in the abnormal odntoblasts of mutant incisor. These results suggest that NFI-C gene causes dissociation of odontoblast and thus abberant odontoblast differentiation and abnormal dentin formation by interfering with the formation of intercellular junctions.

시판우유 및 조제분유의 화학적 성분에 관한 연구

주미란,고영수 漢陽大學校 韓國生活科學硏究所 1998 韓國 生活 科學 硏究 Vol.- No.16

The study was analyzed to testify the excellency among commercial milk and infant formula. In view of these facts, it is of importance comparing chemical compositions. 5 commercial milk and 3 infant formula were purchased in supermarket. 1.Total nucleic acid concentration in commercial milk and infant formula were 1.43W /V% and 2.62W/V%, respectively. 2.Total free sugar concentration in commercial milk(6. 32W. V%) and infant formula (11.68W/V%). 3.Total amino acid concentration was 2. 86% in commercial milk, and 11. 20% in infant formula. 4.In fatty acids, especially DHA in commercial milk was not detected. 5.Major mineral and trace metal analyzed by ICP after wet digestion. The elements examined in the samples were Ca, Mg, K, P, Na in major mineral and Cr, Mn, Fe, Ni, Cu, Zn, As, Cd, Pb in trace metal. In the most of trace metal, infant formula was higher than commercial milk. Cd and Pb were not detected.

Isolation and Partial Characterization of Proteolytic Enzymes from Stems of Pineapples Cultivated in Cheju Island

高榮煥,姜永周 제주대학교 1990 논문집 Vol.31 No.-

A pineapple is one of the major sources of proteolytic enzymes of plant origin which have been widely used in industry. A bromelain was isolated from stems of pineapples cultivated in Cheju island and its characteristics were partially determined. The optimum temperature and pH for its catalytic activity were 62.5℃ and 7.5 , respectively. Crude enzyme preparations lost catalytic activity rapidly above 40℃ and were stable at pH range 5.6-9.0. Coexistence of substrate casein increased heat stability of the bromelain about 25%, thus the enzyme was stable up 45℃. Its value of Km for casein was 0.1%.

식품영양학 전공자를 위한 인턴십 교육 프로그램 개선방안

주나미,윤지영,김옥선,정현아,박상현,고영주,조기원 한국식생활문화학회 2005 韓國食生活文化學會誌 Vol.20 No.1

This study was conducted to evaluate the effect of current dietetic internship program. The survey sample was dietetic practitioners who were charged with a training for dietetic students. The questionnaire requested information about demographic information, experience of guidance for the dietetic student, self evaluation as trainer, fortification items in university education, and difficulties of the internship program. Data was analyzed using SAS for windows(version 6.12), Descriptive statistics were used such as means. standard deviations. frequency distribution and percentages. Comparison of mean differences was analyzed using t-test and ANOVA. Participants identified how effective the current internship program was. Result indicated that the effectiveness was not high(2.81), and under 29 years old age group, under 6 years work experience group. and contracted foodservice group had positive opinion of the program significantly. Under 29 years old age group, evaluated themselves as a good trainer. The results revealed that respondents wanted to be fortified the practical training courses in school curriculum.

막걸리와 물의 첨가비율에 따른 증편의 품질특성

고명수,김상애,최영주 신라대학교 자연과학연구소 2006 自然科學論文集 Vol.15 No.-

본 연구는 막걸리와 물의 첨가비율을 달리하여 증편을 만들어 품질 특성을 검토하였다. 그 결과는 다음과 같다. 1. 증편의 부피변화에서 막걸리 30% 첨가군에서 가장 높게 나타나 발효가 잘 된 것으로 나타났고(p<.0001), 높이에서도 부피가 가장 많이 증가했던 막걸리 30%첨가군이 가장 높게 나타났다(p<.0001). 2. 증편의 pH와 당도변화에서는 pH는 발효가 진행됨에 따라 낮아지는 경향을 보였으며 (p<.0001) 각각의 조건에서 모두 유사함을 나타냈는데 막걸리 함량이 많이 첨가될수록 낮아지는 경향을 보여주었다. 당도에서는 모든 막걸리 첨가군에서 발효시간에 따라 증가하는 경향을 보이다가 감소하였다(p<.0001). 3. 증편의 texture측정에서 견고성(p<.0001), 파쇄성(p<.05), 응집성(p<.05), 껌성(p<.0001),은 막걸리의 첨가량이 증가할수록 유의적으로 높게 나타났고, 반면에 부착성은 막걸리 50%첨가군이 가장 높게 나타났다(p<.05). 탄력성은 막걸리 30% 첨가군이 가장 높게 나타났다(p<.05), 4. 증편의 관능적 특성에서 색의 강도와 기공의 균일한 정도는 첨가군들간의 유의적인 차이를 보였으며, 색은 막걸리 50%첨가군이, 기공의 균일도는 막걸리 30% 첨가군에서 가장 높게 나타났다(p<.0001). Texture에서 부착성(p<.0001)과 탄력성(p<.001)은 첨가군들 간의 유의적인 차이가 나타났고, 그중에서도 막걸리 50% 첨가군이 가장 높게 나타났다. 향미에서는 단맛는 막걸리 60% 첨가군이, 신맛과 술 냄새에서는 막걸리 50% 첨가군이 가장 높게 나타났고, 각각의 첨가군들 간에 유의적인 차이를 보였다(p<.0001). 전체적인 기호도에서는 texture에서 촉촉함, 부착성, 탄력성이 가장 높게 나타난 막걸리 50% 첨가군이 가장 높게 나타났다(p<.001). 5. 기계적 검사와 관능검사간의 상관관계에서 기계적 검사의 견고성은 관능검사의 기공의 균일한 정도와 부의 상관관계를 나타내었고(p<.01), 쓴맛과는 정의 상관관계를 나타내었다(p<.05). 파쇄성은 기공의 균일한 정도와 부의 상관관계를 나타내었다(p<.05). 부착성은 색의 강도, 촉촉함, 탄력성과 높은 부의 상관관계를 나타내었고(p<.01), 부착성과는 정의 상관관계를 나타내었다(p<.05). 탄력성은 색의 강도, 촉촉함, 탄력성과 부의 상관관계를 나타내었다(p<.05). 껌성은 기공의 균일한 정도와 부의 상관관계를 나타내었다. 탄력성은 전체적인 기호도와는 부의 상관관계를 나타내었다. 6. 기계적 검사간의 상관관계에서 견고성은 파쇄성(p<.05), 껌성(p<.0001)과 높은 상관관계를 나타내었고, 파쇄성는 껌성과 정의 상관관계를 나타내었다(p<.01). 부착성은 탄력성과 정의 상관관계를 나타내었다(p<.001), 이상의 결과에서 막걸리와 물의 비율을 달리한 증편의 제조에서 이화학적 특성, 기계적 특성, 관능적 특성을 종합했을때 막걸리 50%첨가군에서 품질이 향상됨을 착인할 수 있었고, 현대인의 기호도를 고려하여 막걸리를 사용함으로써 나타나는 특유의 술 냄새와 신맛을 최소화하기 위한 개발이 요구되어 진다. Jeungpyun was prepared with different ratios of makkulli leaven to water in other to examine the changes in its quality characteristics. The results on the heiht and volume of the samples showed that Jeungpyun prepared with the 30% makkulli showed the highest degree of fermentation. As fermentation continued, pH and saccarinity of Jeungpyun batters droped. As the makkulli ratio incresed, L value was decreased; however, a value and b value were increased. In texture profile analysis, the hardness, fracturability, cohesiveness and gumminess were increased as addition of makkulli was increased, but adhesiveness, springness were decreased. In the sensory test, the 50% makkulli had the strongest color according to the texture, the cell uniformity was shown in the 30% makkulli; the highest adhesiveness and springness were shown in the 50% makkulli. As for flavor, the sweetness was shown in the 60% makkulli and the alcohol smell, sourness were shoen in the 50% makkulli. In the result of the overall desirability test, the 50% makkulli showed the highest values. From the results of this experiment, the 50% makkulli mas the optimum concentrations for the observed improvements in the quality.

化學的으로 再生된 金屬 bracket의 接着强度와 slot幅徑 變化에 關한 硏究

高永三,李東柱 대한치과교정학회 1990 대한치과교정학회지 Vol.20 No.2

The purpose of this study was to measure and compare tensile and shear strength for 4 types of new direct-bonding brackets and same brackets after recycling and to evaluate the change of bracket slot width after recycling. Four types of new direct-bond brackets were bonded to recently extracted human premolar teeth and the tensile and shear strength was measured by Universal Testing Machine. The brackets were recycled by chemical process and the tensile and shear test was repeated. To evaluate the change of the bracket slot width, slot width was measured by the Topcon Universal Measuring Microscope before and after recycling. Following results were obtained: 1. There was no satistically significant difference between the tensile and shear strength of recyed brackets and those of new brackets. 2. In both new and recycled brackets, the tensile and shear strength of perforated base bracket was lower than those of photoetched, foilmesh and contou-lok mesh base brackets.(P<0.01) 3. There was no statistically significant difference in bonding strengths of control group bonded only once and two times. 4. There was no statistically significant difference in the change of the bracket slow width after recycling process. 5. Of the failure, the combination type(58%) in the tensile strength and the tooth adhesive interface(65%) in the shear was the most common type.

역할 기반 접근제어를 이용한 워크플로우 등록소 개발

고영승,주경수 순천향대학교 부설 산업기술연구소 2004 순천향 산업기술연구소논문집 Vol.10 No.1

RBAC(Rle Based Access Control) is more flexible and fine control than the discretionary and mandatory access controls that are currently popular used. Therefore, RBAC will be used in commercial and governmental sectors such as financial, enterprise, and etc. A workflow registry which is developed to share a process definition between enterprises on Web has not function for authorization to users. And Data can be deleted or modified by unauthorized users because all users can handle it. In this paper, we apply a RBAC to a workflow registry. So only authorized users can use functions given a role. Therefore unauthorized users can't delete or modify a data. According to this system, we will increase efficiency and security.

2-Acetylaminofluorene으로 유발된 실험적 간암의 세포 동태학적 연구

금주섭,고영혜,이중달 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.2

Kinetics of hepatocytes in various precancerous and cancer iesions during hepatocarcinorenesis was evaluated in 38 Sprague-Dawley rats fed with 0.075% 2-acetylaminofluorene diet. The rats were sacrificed after 4, 7, 10 days. 2, 3, 4, 6, 8 weeks, and 3, 4, 5, 6, 7, 8 months of the experiment. The lesions were divided as altered cell lesions (clear cell focus, acidophilic cell focus, and basophilic cell focus). regenerating nodules. neoplastic nodules, and hepatocellular carcinomas. Cell kinetics was studied by intraperitoneal bromodexyuridine(BrdU) injection 2 hours before sacrifice. After detection of the BrdU-incorporated nuclei with monoclonal antibody kit, the BrdU labelling index(BLI) was measured as percentage of BrdU labelled cells among about 1000 cells. The liver cells in the altered cell lesions showed significantly increased BLI(clear cell 1.66: acidophilic 2.39: basophilic 2.41) in comparion with normal control(0. 18) or non-lesional hepatocytic lobules (0. 47), and lower BLI than hepatocellular carcinoma(8.61∼27.07) (P<0.05). Regenerating and neoplastic nodules did not differ in their BLI (1.54 and 1.70), and were significantly lower in their proliferating kinetics than acidophilic or basophilic cell foci (P<0.05). It was suggested from this study that the altered cell lesion should be true premalignant lesion, which was composed of at least initiated cells by the chemical carcinogen. The basophilic and acidophilic foci in altered cell lesions were suggestive of close correlation with the hepatocellular carcinoma in that they had greatest proliferative cell kinetics of all the lesions occurred before cancer developed.

지능형 빌딩을 위한 통신실 설치기준

김영오,서태석,양수경,류명주,고대식 牧園大學校 建築·都市硏究센터 1998 建築·都市環境硏究 Vol.6 No.-

본 연구에서는 국내 건축물의 통신실에 대한 운영상의 문제점을 조사·분석하고 효율적인 구내통신 서비스를 위한 통신실의 설계 및 설치기준안을 제시하였다. 국내 업무용 건물의 통신실 설계 및 설치현황을 조사한 결과, 대부분의 통신실 크기가 장비의 설치 및 유지보수시에 필요한 여유공간의 고려가 없이 작게 설치되어 있었으며, 위치 고려의 문제점도 발견되는 등 통신실의 설계 및 설치단계에서 통신실의 중요성이 크게 인식되지 못한 문제점이 있었다. 한편, 통신실에 관한 국내·외 기준을 비교한 결과, 대부분의 선진국에서는 통신실의 설치기준이 자세하게 포함된 구내통신설비의 설계 및 설치 기술표준을 제정, 운용하고 있었지만, 국내에는 통신실에 대한 구체적인 설계 및 설치 표준이 없는 실정이다. 따라서, 본 연구에서는 국내 주거용 및 업무용 구내통신설비 설치표준과 미국의 통신협회인 BICSI 매뉴얼을 기반으로 하고 국내상황을 고려한 통신실의 설계 및 설치 기준안을 작성하였다.