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        Programmed cell death ligand 1 alleviates psoriatic inflammation by suppressing IL-17A production from programmed cell death 1-high T cells

        Kim, J.H.,Choi, Y.J.,Lee, B.H.,Song, M.Y.,Ban, C.Y.,Kim, J.,Park, J.,Kim, S.E.,Kim, T.G.,Park, S.H.,Kim, H.P.,Sung, Y.C.,Kim, S.C.,Shin, E.C. Mosby 2016 The journal of allergy and clinical immunology Vol.137 No.5

        <P>Background: Psoriasis is one of the most common chronic inflammatory diseases of the skin. Recently, IL-17-producing T cells have been shown to play a critical role in psoriatic inflammation. Programmed cell death 1 (PD-1) is a coinhibitory receptor expressed on T cells in various chronic inflammatory diseases; however, the expression and function of PD-1 during psoriatic inflammation have not previously been characterized. Objective: We examined PD-1 expression on IL-17A-producing T cells from imiquimod-treated mice and patients with psoriasis. Additionally, we investigated the therapeutic effect of recombinant programmed cell death ligand 1 (PD-L1) protein on imiquimod-induced psoriatic inflammation. Methods: PD-1 expression on IL-17A-producing gamma delta T cells from imiquimod-treated mice was examined by means of multicolor flow cytometric analysis. In the psoriatic skin of patients, PD-1 and IL-17A expression was analyzed by using immunofluorescence. The therapeutic effect of PD-L1-Fc fusion protein (PD-L1-Fc) was assessed in imiquimod-treated mice ex vivo and in vivo. Results: During imiquimod-induced psoriatic inflammation, PD-1 is overexpressed on CD27(-)V gamma 1(-) gamma delta T cells. Furthermore, PD-1 expression on IL-17A(+) T cells was confirmed in psoriatic skin tissues from patients and imiquimod-treated mice. In the CD27(-)V gamma 1(-) gamma delta T-cell population, V gamma 4(-) gamma delta T cells with V gamma 6 mRNA expression showed a high level of PD-1 expression. Furthermore, these PD-1(hi)V gamma 4(-)(V gamma 6(+)) gamma delta Tcells were specialized for anti-CD3-induced IL-17A production, which was inhibited by PD-L1-Fc treatment. In imiquimod-treated mice PD-L1-Fc reduced psoriatic inflammation when given alone and enhanced the therapeutic effect of anti-p40 when given in combination. Conclusion: PD-1 is overexpressed in IL-17A-producing T cells in both imiquimod-treated mice and patients with psoriasis. Moreover, recombinant PD-L1-Fc alleviates psoriatic inflammation in imiquimod-treated mice.</P>

      • 200 GeV/핵자 유황이온과 핵건판핵의 충돌에 의해 생성된 헬륨 파쇄핵의 극한파쇄 연구

        김동철,송진섭,윤천실,정성헌,박인곤,김종오,김철수,김태연,이승희,조재희,천병구,김재률,김준원,김태익,박명렬,장한일,임인택 慶尙大學校 기초과학연구소 1992 基礎科學硏究所報 Vol.8 No.-

        고에너지 중이온 원자핵과 핵건판의 충돌에서, 200GeV/핵자 유황이온에 의해 생성된 파쇄 헬륨핵(Z=2)의 실험실계의 방출각 분포는 표적핵에 무관한 회귀공식. dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)]로 잘 표현된다. 여기에서 의사신속도 η=-ln[tan(θ/2)]이고, y_b는 실험실계의 입사입자(^32S)의 신속도이다. 이 공식에 의한 적합에서 k=-0.057±0.008로 얻어진다. 즉, 핵건판과 고에너지 중이온의 충돌에서 파쇄 헬륨핵의 exp(η-y_b)의 분포는 "극한파쇄" 현상을 잘 설명하고 있다. The angular distribution of emission angle θ of helium (Z=2) produced in the collisions of incident particles of 200 GeV/nucleon ^32S in nuclear emulsion is well expressed by dN=exp[a+k exp(η-y_b)]d[exp(η-y_b)] where the pseudorapidity is η=-ln[tan(θ/2)], the laboratory system primary rapidity is y_b, and k=-0.057+0.008. The shape of this frequency of occurrence distributions in terms of exp(η-y_b) attests to the validity of the concept of "limiting fragmentation" for helium projectile fragments produced in the projectile fragmentation regions of heavy ion collisions in nuclear emulsion.

      • Pepsin - Cellulase 에 의한 국내산 주요 조사료의 DMD 에 관한 연구 : (3) 주요목초의 세포벽 구성물질과 건물소화율(乾物消化率) (3) CELL WALL CONSTITUENTS AND DRY MATTER DIGESTIBILITY OF SEVERAL PASTURES

        김영길,김대진,맹원재,황태기 한국영양사료학회 1989 韓國營養飼料學會誌 Vol.13 No.4

        主要 牧草의 成熟期別에 따른 成分 및 消化率變化를 評價하기 위하여 禾本科牧草 4種(orchard grass, tall fescue, timothy, perennial ryegrass)과 荳科牧草 3種(alfalfa, ladino clover, led clover)을 採取하여 實施하였다. 이들 牧草를 70℃에서 24時間 건조하고 粉碎하여 1㎜채를 통과시켜 粗蛋白質, 粗灰分, neutral detergent fiber(NDF), acid detergent fiber(ADF), acid defergent lignin(ADL), 그리고 pepsin-cellulase를 이용한 乾物消化率을 測定하고 TDN, DE, ME를 계산하였는데 그 結果를 要約하면 다음과 같다. 1. 粗蛋白質 含量에 있어서 禾本科牧草는 17.80∼19.39% 범위였는데 荳科牧草는 16.66∼22.99% 범위였으며 生育時期의 進行에 따른 蛋白質 含量은 一定期問동안 低下하였으나 그후는 變化가 없었다. 2. 粗灰分 含量에 있어서 禾本科收草는 11.89∼15.06%였고 荳科牧草는 11.57∼14.32%의 범위였고 生育時期의 進行에 따른 粗灰分 含量 차이는 없었다. 3. NDF에 있어서 禾本科牧草 는 53.99∼57.28% 범위였는데 荳科牧草는 40.68∼54.22% 범위였으며 生育時期의 進行에 따라 增加하였다 (p<0.05). 4. ADF에 있어서 禾本收草는 31.47∼36.61% 범위였는데 荳料牧草는 30.28∼36.25%의 범위로 生育時期의 進行에 따라 높았다 (p<0.05). 5. ADL에 있어서 禾本料牧草는 2.30∼3.85% 범위였는데 荳科牧草는 4.21∼6.84%의 범위였으며 生育時期의 進行에 따라 增加하였다 (p<0.05). 6. DMD에 있어서 禾本科牧草는 50.71∼70.40% 범위였으며 荳科牧草는 61.62∼79.19%의 범위로 生育時期의 進行에 따라 減少 하였다 (p<0.05). 7. 牧草類의 可消化養分總量, 可消化에너지, 代謝에너지價는 生育時期의 進行에 따라 減少하는 경향이었다. 8. 禾本科牧草의 DMD 含量(Y)과 粗蛋白質含量(X) 사이에는 Y=1.915X +23.561의 回歸式과(r=0.396, p<0.01)의 相關關係가 있었고 DMD含量(Y)과 ADF(X) 사이에는 Y= -1.475X + 108.538의 回歸式과(r=-0.483 p<0.05)의 相關 이었다. 9. 荳科牧草의 DMD 含量(Y)과 ADL 含量(X)사이에는 Y = -3.856X + 90.531의 回歸式과 (r=-0.893, p<0.01)의 高度의 相關關係와 DMD 含量(Y)과 組蛋白質 含量(X) 사이에는 Y=2.757X + 14.180의 回歸式과(r=0.834, p<0.01)의 相關關係가 있었다. The changes of the chemical components and dry matter digestibily of 4 grasses (orchard grass, tall fescue, timothy and perenial ryegrass) and 3 legumes (alfalfa, ladino clover and red clover) cultivated at the grassland in Yangsan, Kyungsang-namdo province was investigated. They were harvested at varying stages of maturity. Samples were dried at 70℃ for 24 hr. and ground to pass a 1㎜ screen. They were subjected to the determination of the crude protein(N × 6.25), crude ash, neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and dry matter digestibility(DMD) by pepsin-cellulose technique. The energy values(total digestible nutrients, TDN; digestible energy, DE; metabolizable energy, ME) were calculated by DMD of pepsin-cellulose technique. The results obtained were as follows; 1. Crude protein content of grasses and legumes ranged from 17.80% to 19.39% and 16.66%-22.99%, respectively and crude protein had a tendency to decrease with increasing stage of maturity. However, contents of crude protein did not change between middle and late stage in some pastures. 2. The crude ash content of grasses and legumes ranged from 11.89% to 15.06�o and 11.57-14.32% respectively, and crude ash did not vary by stages of maturity. 3. The NDF of grasses and legumes ranged from 53.99% to 57.28% and 40.68%0-54.22%, respectively, and increased by advancing stage of maturity (p<0.05). 4. The ADF of grasses and legumes ranged from 31.47% to 36.25% and 30.28%-36.25%, respectively, and increased by advancing stage of maturity (p<0.05). 5. The ADL of grasses and legumes ranged from 2.3% to 3.85%, respectively, and increased by advancing stage of maturity(p<0.05). 6. The DMD of grasses and legumes ranged from 50.71% to 70.40% and 61.62%-79.19%, respectively, and decreased by advancing stage of maturity (p<0.05). 7. The calculated energy (TDN, DE, ME) values of grasses and legumes decreased by stage of maturity. 8. Regression equation and correlation coefficients of grasses and between DMD (Y) and crude protein (X) were Y=1.915X + 23.561 and r= 0.396 (p<0.05), and of grasses between DMD (Y) and ADF (X) were Y = -1.465X + 108.538 and r=0.483 (p<0.05), respectively. 9. Regression equation and correlation coefficients of legumes between DMD (Y) and ADL (X) were Y = 3.856X + 90.531 and r=-0.893 (p<0.01), and of legumes between DMD (Y) and crude protein (X) were Y =-2.758X + 14.180 and r=0.834 (p<0.01), respectively.

      • Deletion in HSP110 T<sub>17</sub>: correlation with wild-type HSP110 expression and prognostic significance in microsatellite-unstable advanced gastric cancers

        Kim, K.J.,Lee, T.H.,Kim, J.H.,Cho, N.Y.,Kim, W.H.,Kang, G.H. W. B. Saunders Co ; Centrum Philadelphia 2017 Human pathology Vol.67 No.-

        <P>Deletion of the HSP110 T-17 mononucleotide repeat has recently been identified as a prognostic marker that is correlated with wild-type HSP110 (HSP110wt) expression in microsatellite instability-high (MSI-H) colorectal cancers. The aim of this study was to assess the correlation between deletion of the HSP110 T-17 repeat and expression of HSP110wt using DNA testing and immunohistochemistry and to determine the prognostic implications of HSP110 T-17 deletion in MSI-H advanced gastric cancers (GCs). The status of HSP110wt expression was evaluated by immunohistochemistry using an HSP110wt-specific antibody in 142 MSI-H advanced GCs. The size of the HSP110 T-17 repeat deletion was analyzed in 96 MSI-H advanced GCs; deletions were divided into small (0-2 base pairs) and large deletions (3-5 base pairs). Low and high expressions of HSP110wt were detected in 38 (26.8%) and 104 (73.2%) of the 142 cases, respectively. The HSP110 T-17 deletion was observed in 45 (46.9%) of the 96 MSI-H GC samples. Tumors with high expression of HSP110wt showed a tendency to have small or no deletion of HSP110 T-17. In Kaplan-Meier survival analysis, tumors with a large HSP110 T-17 deletion were associated with favorable overall survival and disease-free survival compared with those with small/no deletion of HSP110 T-17. However, HSP110 T-17 deletion size was not an independent prognostic factor in multivariate analysis. In summary, deletion of the HSP110 T-17 repeat was frequently observed in MSI-H GCs, and HSP110 T-17 deletion size was inversely correlated with HSP110wt expression status. Large HSP110 T-17 was not a prognostic indicator in MSI-H GCs. (C) 2017 Elsevier Inc. All rights reserved.</P>

      • SCOPUSKCI등재

        정상 임신에서 임신주기에 따른 갑상선기능의 변화

        김원배,정재훈,윤보현,이석인,김민선,오태근,조보연,이홍규,고창순 대한내분비학회 1994 Endocrinology and metabolism Vol.9 No.3

        It is well known that normal pregnancy is accompanied by a rise in serum concentrations of thyroxine-binding globulin(TBG) and human chorionic gonadotropin (hCG). Alterations of biochemical parameters of thyroid function are recognized during gestation and sensitive tests to evaluate the alterations easily are required. Therefore, a cross-sectional study was undertaken in 140 healthy pregnant women to evaluate the efficacy of free T_4 measured by 2-step RIA compared to other thyroid function tests and to confirm the changes of thyroid function according to the stages of normal pregnancy. The sensitivities of free T_4 index, free T_4(by 2-step RIA), T_3 and TSH were realtively high(99.3%, 93.6%, 92.9%, 83.6%, respectively) compared to those of T_4 and T_3 bead upgake(49.3%, 21.4%) during all stages of pregnancy. There were positive correlations between free T_4 index and free T_4 or total T_4(r=0.68, r=0.72; p$lt;0.001). The values of free T_4 index sharply decreased from 3.22+-0.10(meam +-SEM) during 6th-12th week to an plateau after 16th-20th week of gestation(p$lt;0.01). The serum concentrations free T_4 and T_3 bead uptake also significantly decreased from 1.65+-0.05 ng/dl, 24.7+- 0.7% during 6th-12th week to an plateau after 16th-20th week of gestation, respectively(p$lt;0.001), No differences were found in the changes of serum concentrations of T_3, T_4 and TSH according to the stages of pregnancy. In conclusion, it is adequate to measure some tests including free T_4 index and free T_4 to evaluate thyroid function during pregnancy. The thyroid physiology and changes of thyroid function according to the stages of pregnancy should be considered in the interpretation of thyroid function status during pregnancy(J Kor Soc Endocrinol 9: 183-189, 1994).

      • SCIESCOPUSKCI등재

        Effect of Dietary Copper Sources (Cupric Sulfate and Cupric Methionate) and Concentrations on Performance and Fecal Characteristics in Growing Pigs

        Huang, Y.,Zhou, T.X.,Lee, J.H.,Jang, H.D.,Park, J.C.,Kim, I.H. Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.6

        This study was conducted to assess the effects of organic and inorganic copper on performance in growing pigs. A total of 100 pigs, average age 63 d and initial body weight 21.46${\pm}$1.13 kg, were assigned to five treatment groups. Dietary treatments included i) CON (basal diet, 0 ppm Cu), ii) T1 (basal diet with 67 ppm Cu as cupric sulfate, $CuSO_4$), iii) T2 (basal diet with 134 ppm Cu as $CuSO_4$), iv) T3 (basal diet with 67 ppm Cu as cupric methionate, CuMet) and v) T4 (basal diet with 134 ppm Cu as CuMet). Throughout the entire experimental period, ADG (average daily gain), ADFI (average daily feed intake) and G/F (gain: feed) ratios showed no significant differences. The dry matter digestibility was improved in the T1, T2, T3, and T4 treatments (p<0.05), as compared with CON. Nitrogen digestibility was improved in the T3 treatment group as compared with CON (p<0.05). As compared with the T1 treatment group, fecal pH values were improved in the CON, T3, and T4 treatment groups (p<0.05). Fecal Cu concentrations were significantly lower in the CON, T3, and T4 treatment groups than in T1 and T2 (p<0.05). The incidence of diarrhea was reduced when the pigs were fed on the T2, T3, and T4 diets as compared with CON. In conclusion, diets supplemented with 67 or 134 ppm Cu as CuMet may prove effective in improving nutrient digestibility and fecal pH value in growing pigs, and fecal Cu concentrations may be reduced by CuMet supplementation.

      • 조사료원과 급여수준이 한우의 비육능력(肥育能力) 및 도체성적(屠體成績)에 미치는 영향

        정태영,김종민,이왕열,선우훈희 한국영양사료학회 1994 韓國營養飼料學會誌 Vol.18 No.1

        조사료 급여수준을 20, 20, 10 및 20%(T1, T3)와 40, 30, 20 및 10%(T2, T4)의 4단계로 구분하고, 다시 동일 조사료 급여수준에서 알팔파 큐브와 볏짚 급여구(T1, T2)와 볏짚 단용구(T3, T4)로 나누어 1991년 8월 1일부터 1992년 5월 10일까지 284일간 韓牛 수소 20두를 시험동물로 공시하여 비육능력과 도체성적을 조사하여 얻은 결과는 다음과 같다. 1. 試驗期開 동안의 1일 평균 사료섭취량(DM)은 T1, T2,T3 및 T4구 각각 8.22, 8.45, 8.71 및 8.37㎏이고 TDN은 각각 5.82, 5.92, 6.12 및 5.84㎏으로 볏짚 만을 급여하고 조사료 섭취량이 가장 낮았던 T3에서 가장 높게 나타났다. 2. 出荷時까지의 日當增體量은 T1, T2, T3 및 T4구 각각 0.875, 0.873, 0.932 및 0.843으로 볏짚 만을 급여하고 농후사료 섭취량이 가장 높았던(DM기준 81.2%) T3의 일당증체량이 가장 높았으나 통계적인 유의차는 없었다. 3. 飼料要求率(DM섭취랑/일당증체량)은 T1, T2, T3 및 T4구가 각각 9.38, 9.68, 9.36 및 9.92㎏으로 나타났으며, TDN 이용률은 각각 6.65, 6.78. 6.57 및 6.93으로 DM과 TDN 이용률도 T3구가 가장 좋았고 그 다음이 T1, T2, T4구순이었다. 4. 도체율은 T1, T2, T3 및 T4구가 59.9, 60.1, 61.2 및 60.4%로서 처리구간 차이가 없었으머, 도체중에 대한 거래 정육률도 각각 76.4, 77.4. 77.9 및 75.6%로서 차이는 보이지 않았다. 갈비뼈를 제외한 뼈의 함량은 T1, 배최장근 단면적은 T2에서 가장 높게 나타났으나 유의차는 없었다. 5. 육량등급은 모든 처리구에서 B등급이었으며, 육질등급은 조사료 섭취량이 낮았던 T1과 T3구에서 1등급이 1두씩, 그리고 T1구와 조사료 섭취량이 높았던 T4구에서 3등급이 1두 있었다. 나머지는 모두 2등급을 나타내었다. 6. 조수익에서 밑소대와 사료비를 공제한 소득에서는 T3구가 두당 849,000원으로서 T1, T2, T4에 비하여 각각 45, 95, 및 160천원이 더 높았다. This experiment was carried out to investigate effects of feeding regime of roughage on beef performance and carcass composition of Korean native bulls. Twenty bulls(average 255㎏ BW) were used for this experiment from August 1, 1991 to May 10, 1992 for 284 days. The animal were fed two types of roughage sources : alfalfa cube and rice straw at 50 : 50 ratio (T1 and T2) or rice straw only (T3 and T4). The bulls were divided into two groups and treated with different roughage levels during four stages : 20, 20, 20, and 20% (T1 and T3) and 40, 30, 20 and 10% (T2 and T4) at growing, early, mid and last fattening period, respectively. The results obtained are summarized as follows : 1. The average daily feed intakes of T1, T2, T3 and T4 were 8.22, 8.45, 8.71, and 8.37 ㎏, respectively, during the overall experimental period. Average daily TDN intakes of T1, T2, T3 and T4 were 5.82, 5.92, 6.12, and 5.84 ㎏, respectively. There was a tendency that the intakes of DM and TDN of T3 were slightly greater compared to the others. 2. During the overall experimental period, the daily gains of T1, T2, T3 and T4 were 0.875, 0.873, 0.932 and 0.843 ㎏, respectively. Even though there were no significant differences in daily gains among treatments, T3 group which are fed 20% rice straw of the diet through out whole period showed the highest gains. 3. Feed conversion (㎏ feed/㎏ gain) of T1, T2, T3 and T4 were 9.38, 9.68, 9.36 and 9.92, respectively, during the overall experimental period. DM and TDN conversions to ㎏ gain were lower in the order of the T3, T1, T2 and T4 without a significant difference. 4. The dressing percentages of T1, T2, T3 and T4 wae 59.9, 60.1, 61.2 and 60.4%, respectively. Retailed cut yields of T1, T2, T3 and T4 were 76.4 77.4, 77.9 and 75.6%, respectively. T3 treatment showed the highest dressing percentage and retailed cut yields compared to other groups without significant differences. Percentage of bone, body fat and loin eye area did not show any significant trends among treatments. 5. No detectable differences were found in the quantity and quality of meat among treatments. 6. T3 group showed the highest income over feed cost [Gross income-(cattle+feeds)] comprising 849,000 won per head.

      • KCI등재

        (Nd₁-xRx)₂Fe₁₄B (R=Y, Pr)의 자기특성

        김만중(M. J. Kim),김윤배(Y. B Kim),김희태(H. T. Kim),김택기(T. K. Kim) 한국자기학회 1998 韓國磁氣學會誌 Vol.8 No.5

        자장중 정렬된(Nd_(1-x)Rx)₂Fe₁₄B (R=Y, Pr) 다결정 분말을 사용하여 스핀재배열현상과 결정자기이방성을 연구하였다. 이 화합물에서 스핀재배열온도(T_(SR))는 R=Pr인 경우 0≤x≤0.75의 조성범위에서 Pr 치환량이 증가함에 따라 △T_(SR) = -1.35 K/Pr at.%의 비율로 단순 감소하였으나 R=Y인 경우에는 초기 소량의 감소 후 다시 약간 증가한다. 4.2K에서 스핀재배열각(SRA)은 Y과 Pr 치환량의 증가에 따라 0≤x≤0.5의 범위에서 △SRA = -0.073°/Y at.%와 △SRA = -0.258°/Pr at.% 비율로 감소한다. 4.2 K에서 결정자기이방성상수의 조사결과 R=Y인 경우 x=0.9 이상에서 R=Pr인 경우에는 x=0.8 이상에서 스핀재배열현상이 사라질 것으로 예측되었다. Spin reorientation and magnetocrystalline anisotropy of magnetically aligned (Nd_(1-x)Rx)₂Fe₁₄B (R=Y, Pr) power were studied. The spin reorientation temperature (T_(SR)) of (Nd_(1-x)Pr_x)₂Fe₁₄B decreases linearly by increasing Pr-substitution with the ratio of △T_(SR)=-1.35 K/Pr at.% in composition range of 0≤x≤0.75. The spin reorientation temperature of (Nd_(1-x)Yx)₂Fe₁₄B decreases by increasing Pr-substitution to 118 K (x = 0.5) then increases to 122 K (x = 0.75). The spin reorientation angle at 4.2 K decreases by increasing rare earth substitution with the ratio of △SRA = -0.073°/Y at.% and △SRA = -0.258°/Pr at.% in composition range of 0≤x≤0.5. The spin reorientation is expected to disappear at x≥0.9 in case of (Nd_(1-x)Yx)₂Fe₁₄B and at x≥0.8 in case of (Nd_(1-x)Pr_x)₂Fe₁₄B.

      • KCI등재

        농촌형 녹색마을에 신재생에너지 보급을 위한 시설재배 및 농업기계의 CO2 배출량 분석

        김종구(J.G. Kim),유영선(Y.S. Ryou),강연구(Y.K. Kang),김영화(Y.H. Kim),장재경(J.K. Jang),김현태(H.T. Kim),서광욱(K.W. Seo),이승기(S.K. Lee),조희제(H.J. Cho),강지원(J.W. Kang) 유기성자원학회 2011 유기물자원화 Vol.19 No.1

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        화석에너지 의존도를 줄이면서 CO2 배출량을 낮추기 위하여 정부에서는 녹색마을을 선정하고 에너지자급률을 40% 수준으로 높이려는 계획을 추진 중이다. 본 연구는 각 농업 분야 중에서 농기계의 사용과 재배 시설에 있어서의 에너지 사용량을 파악하고 이를 바이오디젤로 대체하였을 때의 CO2 저감수준을 분석하고자 하였다. 이를 위하여, 농업 각 분야별 에너지 소비수준의 분석, 그리고 실천 가능한 신재생 에너지원의 선정이 요구된다. 경종재배의 전체 연간온실가스 배출량은 5,667,258 t-CO2이고, 그 중 시설 부문은 4,932,607 t-CO2인 것으로 분석되었으며, 농업시설 부문 중 에너지원별로 보면 경유가 3,105,707t-CO2, 중유가 1,370,578 t-CO2를 배출하는 것으로 분석되었다. 우리나라 시설작물의 단위 면적당 온실가스 평균배출량은 29,418 t-CO2/ha인 것으로 나타났다. 농기계별 2007년 총에너지소비량을 살펴보면 트랙터가 284,763 kL로 가장 높게 나타났으며, 동력 경운기221,314 kL, 곡물건조기 145,524 kL, 콤바인 72,537 kL 등의 순이었다. 전라북도 G시를 대상으로 이용 중인 시설재배와 농업기계의 이산화탄소 배출량을 비교분석한 결과,바이오디젤로 전환하면 약 7% 정도의 CO2 감소효과가 있는 것으로 분석되었다. In order to reduce dependence on the fossil fuels and CO2 gas emission in farming activities, the government has pushed ahead with making the self-sufficiency of farming energy up 40% level in green villages. The objectives of this study are to survey the energy consumption of horticultural facilities or agricultural machineries, and to analyze the reduced CO2 gas emission level from fossil fuel to bio-diesel fuel. For the implement of this study, it is necessary to analyze the energy consumption level in the various sector of farming activities, and available renewable energy sources should be selected. Annual total CO2 gas emission in the tillage farming sector was analyzed as 5,667,258 t-CO2 and that in the horticultural facilities occupied 4,932,607 t-CO2, while the CO2 gas emission level of diesel fuel was 3,105,707 t-CO2, and that of the heavy oil showed 1,370,578 t-CO2. The average CO2 gas emission level of horticultural facilities in the country was analyzed as 29,418 t-CO2/ha. Among the total energy consumption of agricultural machineries, tractor used 284,763kL, power tiller spent 221,314 kL, grain drier consumed 145,524kL and combine tractor expend 72,537kL. From the comparison of CO2 gas emission level between fossil fuel and bio-diesel fuel for the horticultural facilities or agricultural machinery in G-City, Jeonbuk Province, the CO2 gas emission level can be reduced by 7% through replacing the fuel from fossil to biodiesel.

      • Tsukamurella carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete

        Park, S. W.,Kim, S. M.,Park, S. T.,Kim, Y. M. Microbiology Society 2009 International journal of systematic and evolutiona Vol.59 No.6

        <P>A Gram-positive, slightly acid-alcohol-fast, carbon monoxide-oxidizing bacterium, strain Y2(T), was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence comparative analyses, strain Y2(T) was shown to belong to the genus Tsukamurella and was most closely related to Tsukamurella tyrosinosolvens DSM 44234(T) (GenBank accession no. AY238514; 99.8 %). The predominant fatty acids were C(18 : 1)omega9c and C(16 : 0). The cell-wall peptidoglycan of strain Y2(T) contained meso-diaminopimelic acid as the diagnostic diamino acid. Strain Y2(T) contained galactose and arabinose as the whole cell sugars. The DNA G+C content was 77 mol%. The DNA-DNA relatedness value between strain Y2(T) and T. tyrosinosolvens DSM 44234(T) was 62.7 %. Based on the combination of the carbon source utilization pattern, fatty acid profile, cell-wall chemotype, DNA G+C content and DNA-DNA hybridization experiments, it is proposed that strain Y2(T) (=KCCM 42885(T)=JCM 15482(T)) represents the type strain of a novel species, Tsukamurella carboxydivorans sp. nov.</P>

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