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Lepidopteran Insect Pests on Grape, Vitis vinifera L., from Korea
Sora Kim,Wonhoon Lee,Sun-Kook Kim,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
Until now, seven species, Acosmeryx nega, Herpetogramma luctuosalis, Ostrinia furnacalis, Endoclyta excrescens, Spodoptera litura, Paranthrene regalis, and Nippoptilia vitis, have been known as lepidopteran grape pests in Korea (Woo, 1980; Lee, 1981; Kim, 1991; NIAST, 2002; Korea grape community, 2009). We discovered thirteen additional species belonging to eight families from Gwangwon and Chungbuk provinces, Korea. Additionally, we corrected the previous records of Deuterocopus albipunctatus and Nppoptilia vitis. They were identified by adult external or genitalic characters first, and also confirmed by the COI marker, compared with two public databases, NCBI and BOLD.
DNA barcoding of the stem borer, Stathmopoda spp. (Lepidoptera: Stathmopodidae)
Sora Kim,Wonhoon Lee,Yerim Lee,Seunghwan Lee 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
The mitochondrial COI gene has proven successful for identifying the lepidopteran species. In addition, this gene has been applied to recognize cryptic species and confirm a polyphagous species as one species. However, it has been also reported misidentification in the COI gene. As a result, some researchers have suggested the use of additional genes in species identification, especially, nuclear genes. In this study, we observed that a nuclear EF1agene is better than the mitochondrial COI gene in recognizing Stathmopoda species. For example, among the all species, 47 individuals of nine species, a large intraspecific pairwise difference were detected, up to 15.0% in universal barcoding regions of the COI gene, 22.0% in other part of the COI gene; however, 2.0% in EF1a. In contrast, Stathmopoda sp2 and S. commoda were separated into several clusters in each different COI regions, and some individuals of S. auriferella and S. commoda, were closer to each other separating from the cluster of the same species. But, we get clear results from the EF1a, Stathmopoda species well clustered and could be distinguished.
Sora Kim,Wonhoon Lee,Sunghoon Jung,Bong-Kyu Byun,Bong-Woo Lee,Seunghwan Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10
Systematic studies within the family Pterophoridae have been determined by external morpholgical characteristics (Yano 1963, Spuler 1910, Meyrick 1910). However, it is sometimes to be the result of species misplacement by the variation, depending on geographic isolation and genetic drift (Moran, 1986; Shufran et al., 2000; Anstead et al., 2002; Margaritopoulos et al., 2006). The genus Platyptilia Hübner [1925] is one of the largest genera of the family comprising more than 100 species according to the World’s Catalog by Gielis (2003) mainly distributed from Palaearctic and Afrotropical regions. Within the genus, Platyptilia ignifera has been suspected as very differentiated from other congeneric species in the morphological characters. In this study, to ascertain a placement of the species, we performed molecular analysis with one mitochondrial gene, COI and one nuclear gene, 28S, and morphometric analysis based on six ratios characters using principal components analysis (PCA). The sequences of the two genes, COI, 28S, implied that Platyptilia ignifera separated from Platyptilia spp. In addition, P. ignifera exhibited morphological characteristics distinct from other congeneric species. Based on these results, we propose that Neoplatyptilia gen. nov., for Platyptilia ignifera.
김원훈(Wonhoon Kim),이방업(Bangeop Lee),구송회(Songhoe Koo),이원복(Wonbok Lee) 한국추진공학회 2010 한국추진공학회 학술대회논문집 Vol.2010 No.11
본 논문에서는 서방에서의 격막형 펄스분리장치를 적용한 다중펄스 로켓모타 기술개발 현황과 소요기술을 분석하였으며, 전방 및 후방 점화 시스템 방식의 이중펄스 로켓모타에 대한 개념설계 결과를 제시하고 있다. In this paper, the present status of research, development, and required technologies for the thermal barrier type dual-pulse rocket motor of the advanced countries was analysed. The result of the conceptual design of the forward or backward ignition systems was also demonstrated.
Present and future of management of invasive exotic insects in Korea
Hyojoong Kim,Dong Eon Kim,Wonhoon Lee,Sunghoon Jung,Wang-Hee Lee,Ki-Jeong Hong,Seunghwan Lee 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
Invasive exotic insects are rapidly increasing and spreading in a short period of time, threatening the ecosystem. Now, we need technology to monitor the dispersal of exotic insects and to strengthen the ecosystem risk assessment. The United States, Europe, and other countries are fully equipped with a biological control system for threats to biodiversity through systematic investigation and database construction, but Korea is not. Risk assessment and development of a dispersal/change prediction model are essential to maintain a safe ecosystem from invasive exotic species. This research group is carrying out a study to minimize the ecological damage by developing a prediction model of dispersal/change for invading alien insect species, a risk assessment technology and a response manual for management. I would like to look into the current state of exotic insect invasion through this small group meeting, and look for directions to study in the future.
Family Pterophoridae (Lepidoptera: Pterophoroidea) in Korea
Sora Kim,Kyu-Tek Park,Bong-Kyu Byun,Bong-Woo Lee,Hyojoong Kim,Sunghoon Jung,Wonhoon Lee,Seunghwan Lee 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10
The family Pterophoridae (Lepidoptera), plume moths, consist of 1,136 species listed in the ‘world catalogue’ by Gielis (2003), and a few more described since then. Approximately 250 species are known in the Palaearctic Regions (Arenberger, 1995). In this study, 35 species of 21 genera have been reviewed from Korea (Matsumura 1931, 1938; Zoological Society of Korea 1968; Park 1983; Arenberger 1991; Kim et al., 2010a, 2010b, 2012a, 2012b). We suggest the problematic species, Platyptilia ignifera, is morphologically and genetically separated from congeneric species supporting the possibility as a new genus, and employ the classification system of Arenberger (1995) rather than that of Gielis (1993) using parsimony based on morphological characters.
김원훈 ( Wonhoon Kim ),임종호 ( Jongho Lim ),강민주 ( Minjoo Kang ),노충환 ( Choong Hwan Noh ),이경준 ( Kyeong-jun Lee ) 한국수산과학회 2023 한국수산과학회지 Vol.56 No.6
The effects of dietary mannan oligosaccharides, Lactobacillus plantarum, Bacillus subtilis, and Bacillus lichenifor-mis supplementation on hybrid grouper Epinephelus akaara ♀×Epinephelus lanceolatus ♂ were evaluated. The fish were fed a basal diet and five other diets consisting of 0.6% mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis and mixture of each 0.15% prebiotic and all the probiotics (designated as MOS, LP, BS, BL, and SYN) for 56 days. Growth performance and feed utilization showed no significant differences among all experi-mental groups. Lipid level of whole-body was significantly high in MOS and BL groups. Plasma aspartate amino-transferase was significantly low in BL and SYN groups. Nitro-blue tetrazolium, lysozyme and anti-protease, and glutathione peroxidase in BS, SYN, and all probiotic groups, respectively, were significantly high. Intestinal Vibrio bacteria was significantly low in all probiotic and SYN groups. Gene expression of interleukin-1ß and interleukin-10 in SYN group; transforming growth factor β2 in MOS and BS groups, toll-like receptor 2-2 in BS and BL groups; and C-type lectin in MOS, LP and SYN groups were significantly upregulated. Our findings indicate that mannan oligosaccharides, L. plantarum, B. subtilis, and B. licheniformis could improve innate immunity, antioxidant capac-ity, anti-inflammation, and intestinal microbiota of hybrid grouper.
Reporting the possibility of invasive populations of Anoplophora spp. in Korea
Moo-Sung Kim,Kyong Young Kim,Hyobin Lee,Wonhoon Lee,Ki-Jeong Hong 한국응용곤충학회 2019 한국응용곤충학회 학술대회논문집 Vol.2019 No.04
In Korea, two species, Anoplophora chinensis and Anoplophora glabripennis, have been recorded until now. Recently, outbreaks of Anoplophora spp. were reported locally in Seoul, Ulsan, Incheon, Gimhae, and Jeonju from 2015 to 2017. In this study, Anoplophora spp. were collected in the four regions, Ulsan, Incheon, Gimhae, and Jeonju, and their morphological and molecular characteristics were compared with those of Anoplophora spp. collected from several local regions. Twelve COI sequences of the Incheon/Gimhae/Ulsan/Jeonju populations in A. glabripennis were identical to KY357648, KY357652, and KY357651, and two COI sequences of Ulsan populations in A. chinensis were identical to AB439163. These sequences were generated from Chinese and Japanese Anoplophora samples by foreign researchers, suggesting the possibility of the invasion of Chinese and Japanese populations of Anoplophora spp. into Korea.