Original Article : Geographic difference of epidemiological features of HCV infection in Korea

( Kyung Ah Kim ),( Sook Hyang Jeong ),( Eun Sun Jang ),( Young Seok Kim3 ),( Youn Jae Lee ),( Eun Uk Jung ),( In Hee Kim ),( Sung Bum Cho ),( Mee Kyung Kee ),( Chun Kang ) 대한간학회 2014 Clinical and Molecular Hepatology(대한간학회지) Vol.20 No.4

Background/Aims: The prevalence of hepatitis C virus (HCV) infection in Korea exhibits significant geographic variation, with it being higher in Busan and Jeonam than in other areas. The reason for this intranational geographic difference was investigated in this study by conducting a comparative analysis of the risk factors related to HCV infection among three geographic areas: the capital (Seoul), Busan, and the province of Jeolla. Methods: In total, 990 patients with chronic HCV infection were prospectively enrolled at 5 university hospitals located in Seoul (n=374), Busan (n=264), and Jeolla (n=352). A standardized questionnaire survey on the risk factors for HCV infection was administered to these three groups of patients, and a comparative analysis of the findings was performed. Results: The analysis revealed significant regional differences in exposure to the risk factors of HCV infection. By comparison with patients in Seoul as a control group in the multivariate analysis, patients in Busan had significantly more experience of invasive medical procedures, acupuncture, cosmetic procedures, and multiple sex partners. In contrast, patients in Jeolla were significantly older, and they had a higher prevalence of hepatocellular carcinoma, a lower prevalence of multiple sex partners, and had experienced fewer invasive procedures. Conclusions: There was a significant geographic difference in the exposure to potential risk factors of HCV infection between patients from the three studied regions. This may explain the regional variation of the prevalence of HCV infection in Korea, and should be taken into account when planning strategies for the prevention and management of HCV infection. (Clin Mol Hepatol 2014;20:361-367)

아동청소년 교우관계문제검사의 요인구조

최선희,김종미,홍상황,Choi, Sun-Hee,Kim, Jong-Mee,Hong, Sang-Hwang 한국초등상담교육학회 2011 초등상담연구 Vol.10 No.2

본 연구는 아동청소년 교우관계문제검사(KIPR)에 잠재되어 있는 요인구조를 밝히고 검증하여 이 검사의 심리적 특성을 밝히고 해석적 유용성을 높이는데 목적이 있다. 이를 위해 경남지역 3개 초등학교 4~6학년 아동 616명을 대상으로 아동청소년 교우관계문제검사를 실시하였고 그 중 578명의 자료를 분석하였다. 연구결과를 요약하면 다음과 같다. 첫째, 3요인과 4요인 모형을 설정하여 탐색적 요인분석을 실시한 결과 3요인 모형이 적합한 것으로 나타났다. 3요인 모형에서 요인 1은 아동청소년 교우관계문제검사의 비주장성(C5), 사회적 억제(C4), 냉담(C3), 과순응성(C6) 원형척도의 문항들로 구성되어 있어서 "자신감 결여와 사회적 위축", 요인 2는 통제지배(C1), 자기중심성(C2) 원형척도의 문항들로 구성되어 있어서 "공격성과 타인조종", 요인 3은 자기희생(C7), 과관여(C8), 과순응성(C6) 원형척도의 문항들로 구성되어 있어서 "과잉배려"로 명명하였다. 둘째, 확인적 요인분석을 실시한 결과 3요인 모형은 4요인 모형보다 수집된 자료를 잘 반영하고 있었고(RMSEA=.054), 적합도는 만족할 수준은 아니지만 수용할 수 있는 수준이었고(CFI=.786), 모형의 간명성 또한 적합한 것으로 나타났다(PCFI=.733). 본 연구에서는 교우관계원형에서 상관계수가 높은 인접한 척도끼리는 동일한 요인으로 묶여지는 것을 확인하였고 이를 통해 8개의 원형척도를 망라하는 상위의 대표적인 요인척도를 구성하는데 필요한 근거를 제공하여, 이 검사의 해석적 유용성을 높였다는 점에서 의의가 있다고 생각된다. The purpose of this study is to reveal and confirm the factor structure inherent in the Korean inventory of peer relationships(KIPR; Sang-Hwang Hong, et al., 2009) in order to strengthen the interpretive usefulness of the test. For this purpose, the inventory was administered to the total of 616 children in the fourth through sixth grades in three elementary schools located in Gyungnam area, and the data from 578 of them were analyzed. The results of the study can be summarized as follows. First, as a result of the exploratory factor analysis of 3-factor and 4-factor models, the 3-factor model was found to be more appropriate. In the 3-factor model, the factor 1 is named "Lack of Self-Confidence and Social Withdrawal" as it consists of such circumplex scale items as Non-Assertiveness (C5), Social Avoidance (C4), the Cold (C3), and Too Submissive (C6). The factor 2 is named "Too Aggressive and Controlling" and consists of items Too Controlling (C1) and Hard to be Supportive (C2). The factor 3 is named "Too Caring" and consists of items Too Responsible (C7), Over Involvement (C8), and Too Submissive (C6). Second, as a result of the confirmatory factor analysis, the 3-factor model, in comparison to the 4-factor model, was found to better reflect the collected data(RMSEA=.054), its goodness of fit was not satisfactory but within the acceptable range(CFI=.786), and its power of simplicity and clarity was also adequate(PCFI=.733). In the current study, through the exploratory and confirmatory factor analyses of scores from the Korean inventory of peer relationships circumplex scales, scales within the peer relationship circumplex model that are near each other and are highly correlated were confirmed to be grouped together as same factors. I believe the main merit of the study lies in the above result having provided the necessary foundation for forming the superordinate and representative scale that encompasses the eight circumplex scales, which enhances the test's interpretive usefulness.

Novel antimutagenic Pigment produced by Bacillus licheniformis SSA3

Kim, Jong Kyu,Park, Sun Mee,Lee, Sang Jun 한국미생물 · 생명공학회 1995 Journal of microbiology and biotechnology Vol.5 No.1

We discovered that the Bacillus licheniformis SSA3, fermenting traditional Korean soy sauce and soybean paste, involved in the synthesis of a dark-brown pigment. This pigment produced in the minimal medium supplemented with tyrosine only as precursor. We showed that this pigment is novel, and differed from melanoidin and melanin, and an antimutagenic substance.

Differential disruption of autoinhibition and defect in assembly of cytoskeleton during cell division decide the fate of human <i>DIAPH1</i>-related cytoskeletopathy

Kim, Bong Jik,Ueyama, Takehiko,Miyoshi, Takushi,Lee, Seungmin,Han, Jin Hee,Park, Hye-Rim,Kim, Ah Reum,Oh, Jayoung,Kim, Min Young,Kang, Yong Seok,Oh, Doo Yi,Yun, Jiwon,Hwang, Sang Mee,Kim, Nayoung K D BMJ Publishing Group Ltd 2019 Journal of medical genetics Vol.56 No.12

<P><B>Background</B></P><P>Diaphanous-related formin 1 (DIA1), which assembles the unbranched actin microfilament and microtubule cytoskeleton, is encoded by <I>DIAPH1</I>. Constitutive activation by the disruption of autoinhibitory interactions between the N-terminal diaphanous inhibitory domain (DID) and C-terminal diaphanous autoregulatory domain (DAD) dysregulates DIA1, resulting in both hearing loss and blood cell abnormalities.</P><P><B>Methods and results</B></P><P>Here, we report the first constitutively active mutant in the DID (p.A265S) of humans with only hearing loss and not blood cell abnormality through whole exome sequencing. The previously reported DAD mutants and our DID mutant (p.A265S) shared the finding of diminished autoinhibitory interaction, abnormally upregulated actin polymerisation activity and increased localisations at the plasma membrane. However, the obvious defect in the DIA1-driven assembly of cytoskeleton ‘during cell division’ was only from the DAD mutants, not from p.A265S, which did not show any blood cell abnormality. We also evaluated the five DID mutants in the hydrophobic pocket since four of these five additional mutants were predicted to critically disrupt interaction between the DID and DAD. These additional pathogenic DID mutants revealed varying degrees of defect in the DIA1-driven cytoskeleton assembly, including nearly normal phenotype during cell division as well as obvious impaired autoinhibition, again coinciding with our key observation in DIA1 mutant (p.A265S) in the DID.</P><P><B>Conclusion</B></P><P>Here, we report the first mutant in the DID of humans with only hearing loss. The differential cell biological phenotypes of DIA1 during cell division appear to be potential determinants of the clinical severity of <I>DIAPH1-</I>related cytoskeletopathy in humans.</P>

Bacterial Density-dependent but Vulnibactin-independent Utilization of Transferrin-bound Iron by Vibrio vulnificus

Kim, Choon-Mee,Jeon, Ho-Jong,Shin, Sung-Heui 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Vibrio vuInificus is a gram-negative halophilic and ferrophilic bacterium that causes a fatal septicemia in patients with underlying diseases, It has been known that the vimlence of V. vulnificus is closely associated with the ability to use transfen-in-bound iron (TBI) which is exclusively dependent on vulnibactin production. However, we have found that V. vulnificus can also use TBI in the bacterial density-dependent but vulmbactin-mdependent manner. To determine this, we used the wild-type V. vulnificus MO6-24/O strain, the fur-deletional mutant CMM2303 strain that produces vulnibactin to high levels and the vzs-insertional mutant CMM2301 strain that can not produce vulnibactin. The CMM2303 strain used TBI more efficiently than the MO6-24/O strains. Both strains used TBI more efficiently at the initial bacterial density of 1x10^(6) cfu/㎖ than at the initial bacterial density of 1x10³ cfu/㎖. In contrast, the CMM2301 strain could not use TBI at the initial bacterial density of 1x10³ cfu/㎖, but it could also use TBI at the initial bacterial density of 1x10^(6) cfu/㎖ although the level was far lower than those of the MO6-24/O and CMM2303 strains. These results indicate that V. vulnificus basically uses TBI in the vulnibactin-dependent manner, and it can additionally use TBI in the bacterial densityependent but vulnibactin-independent manner, suggesting that other iron-uptake systems besides the vulnibactin-mediated iron-uptake system may also be involved in TBI utilization by V. vulnificus.

QOS-Based Multiplexing Algorithm for Broadband ATM Access Network in B-ISDN

Sung Won Sohn,Jong Oh Kim,Yoon Mee Doh,Jun Kyun Choi 대한전자공학회 1993 JC-CNSS : Joint Conference on Communications, Netw Vol.6 No.-

Rapid prenatal diagnosis of spinocerebellar ataxia type 3 by using fluorescent PCR

Kim, Do-Jin,Park, So-Yeon,Kim, Mi-Jin,Lee, Moon-Hee,Shim, Sung-Han,Ryu, Hyun-Mee Korean Society of Medical Genetics 2007 대한의학유전학회지 Vol.4 No.1

척수소뇌성 실조증3는 신경세포의 손상으로 인해 생기는 질병으로 염색체14q32.1지역에 반복적인 CAG 삼염기 서열이 증가하면서 일어나는 것으로 알려져 있다. 본 증례는 척수소뇌성 실조증3으로 진단을 받은 부부에서 자연 임신한 태아를 산전진단한 경우로서 형광으로 포식된 표지자를 이용하여 CAG 지역을 증폭하여 빠르고 정확하게 반복수를 확인하는 방법을 이용하였다. 남편의 경우 CAG반복을 넘는 69개의 반복과 정상인 27개의 반복된 유전자를 갖고 있는 것으로 확인하였으며, 산모의 경우 정상인 26과 32개의 반복된 유전자를 갖고 있는 것으로 확인하였다. 태아는 부계의 27과 모계의 26개를 갖는 정상 유전자를 물려 받은 것으로 확인되어 건강한 아기를 분만하였다. 형광을 이용한 진단방법은 방사능을 사용하는 방법에 비해 안전하고 빠른 진단을 할 수 있으며 시료 채취 후 5-6시간 안에 정확하게 결과를 확인할 수 있는 방법이라 생각된다. Spinocerebellar Ataxia Type 3 (SCA 3) is a rare autosomal dominative disorder in which one of the neurodegenerative disorders is caused by a CAG repeat expansion on chromosome 14q32.1. The age at onset of disease is related to the size of the expanded CAG repeat. We present the prenatal diagnosis of SCA3 in a woman whose husband was known to carry an unstable CAG repeat expansion in the MJD gene. The diagnosis was made using PCR with a fluorescent probe for an expanded MJD allele. The normal ranges of (CAG)n of SCA3 are 14~38 repeats. The husband, who had a family history of SCA 3, has an expanded allele of 69 CAG repeats with a normal allele of 27 repeats. His wife had two normal alleles with 26 and 32 CAG repeats. The fetus had two normal alleles with 26 and 27 CAG repeats; consequently, the baby w as healthy. We report a case of prenatal diagnosis of SCA3 using a fluorescent PCR which is rapid and accurate.

Gα12 ablation exacerbates liver steatosis and obesity by suppressing USP22/SIRT1-regulated mitochondrial respiration

Kim, Tae Hyun,Yang, Yoon Mee,Han, Chang Yeob,Koo, Ja Hyun,Oh, Hyunhee,Kim, Su Sung,You, Byoung Hoon,Choi, Young Hee,Park, Tae-Sik,Lee, Chang Ho,Kurose, Hitoshi,Noureddin, Mazen,Seki, Ekihiro,Wan, Yu-J American Society for Clinical Investigation 2018 The Journal of clinical investigation Vol.128 No.12

<i>Vibrio vulnificus</i>metalloprotease VvpE is essentially required for swarming

Kim, Choon-Mee,Park, Ra-Young,Chun, Ho-Jong,Kim, Soo-Young,Rhee, Joon-Haeng,Shin, Sung-Heui Published by Elsevier/North Holland on behalf of t 2007 FEMS microbiology letters Vol.269 No.1

<P>Bacterial swarming constitutes a good in vitro model for surface adherence and colonization, and is accompanied by expressions of virulence factors related to invasiveness. In this study, it was determined that Vibrio vulnificus swarming was abolished by mutation of the vvpE gene encoding a metalloprotease VvpE and this swarming defect was recovered by complementation of the vvpE gene. Expression of the vvpE gene began simultaneously with the beginning of swarming and increased along with expression of the luxS gene encoding the synthase of the precursor of quorum-sensing signal molecule autoinducer 2, and this increased vvpE expression was decreased by mutation of the luxS gene. Moreover, VvpE destroyed IgA and lactoferrins, which are responsible for mucosal immunity. These results suggest that VvpE may play important roles in the surface adherence and colonization of V. vulnificus by facilitating swarming and in the mucosal invasion of V. vulnificus by destroying IgA and lactoferrin.</P>

Quality Characteristics and Content of Polysaccharides in Green Tea Fermented by Monascus pilosus

Kim, Mee-Jung,Kim, Song-Suk,Lee, Sang-Il The Korean Society of Food Science and Nutrition 2012 Preventive Nutrition and Food Science Vol.17 No.4

In this study, we designed a method to manufacture elevated fermented green tea by using Monascus pilosus, which is known as a functional microbe, and observe its antioxidant abilities and quality characteristics. The water-soluble substance (WSS) content of the fermented tea by M. pilosus (FTM) was lower than that of the non-fermented tea (NFT), although the alcohol-insoluble substance (AIS) content of the FTM was higher than that of NTM. On the other hand, the fractionated distilled water-soluble polysaccharide (DWSP), CDTA-soluble polysaccharides (CDSP), sodium carbonate-soluble polysaccharide (SCSP) and KOH soluble hemicellulose (HC) obtained from the AIS of the FTM was markedly higher than that of NFT. In the antioxidant parameters, the electron donating ability of all fractions, except HC, extracted from FTM was higher than that of NFT, and iron chelating ability of all fractions, except CDSP, extracted from FTM was higher than that of NFT. Whereas the DWSP and SCSP obtained from the FTM were higher than that of NFT, the activity of the HC fraction from both NFT and the FTM could not be detected. In addition, the xanthin oxidase (XO) inhibitory activities of the DWSP, CDSP and the SCSP obtained from the NFT were significantly higher than that of FTM, the aldehyde oxidase (AO) inhibitory activities of the DWSP and SCSP extracted from the FTM were markedly higher than that of the NFT. Meanwhile, the acceptance of NFT and FTM had no significant difference, while the quality of aroma, taste and mouthfeel of the FTM was higher than that of NFT. These results suggest that the post-fermented tea by Monascus microorganisms may be responsible for functional components as well as contribute to the improvement of the tea quality.