가압가열 및 microwave에 의한 중력분 반죽 gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won Kim),안동현(Dong-Hyun 한국식품과학회 2012 한국식품과학회지 Vol.44 No.1

본 연구에서는 가압가열 및 microwave 처리에 의한 중력분 반죽 추출물 내의 gliadin 단백질의 항원성 변화에 대해 살펴보았다. 중력분 반죽에 가압가열과 microwave를 단독 또는 병행으로 처리하여 ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였으며, 가압가열 처리에 의해서 anti-gliadin IgG 항체와 gliadin과의 결합력이 다소 감소한 것을 확인하였다. 특히 30 min 이상 처리시 더욱 감소한 것으로 나타났으며, SDS-PAGE와 immunoblotting 결과에서도 gliadin band의 강도가 약해지고 항체와의 반응도 나타나지 않았다. Microwave 처리의 경우, 5 min 이상 처리시 일부 gliadin 단백질의 소실이 관찰되었으나, 항원성에는 큰 변화가 없었다. 또한 가압가열 및 microwave 병행 처리에 의해 항원-항체 결합력이 더욱 감소되었으며, 특히 가압가열 50 min, microwave 10 min 처리시 약 35.0%로 감소되었다. 이상의 결과를 통해 가압가열 처리에 의해 중력분 반죽 추출물 내 gliadin의 항원성이 감소되는 것을 확인하였으며, microwave와 병행 처리하는 경우, 더욱 감소하는 것을 확인하였다. The aim of this study was to determine the optimal physical treatment to reduce the antigenicity of gliadin in wheat dough. Medium wheat dough was treated with an autoclave (5, 10, 30, and 50 min at 121℃, 1 atm), a microwave (1, 5, and 10 min) or both (10, 30, and 50 min/5, 10 min). The proteins in the dough extracts were analyzed by SDS-PAGE and the binding ability of anti-gliadin IgG to gliadin was examined by ci-ELISA and immunoblotting. Results showed that the ability of anti-gliadin IgG to bind to gliadin in wheat dough treated with an autoclave alone or in combination with a microwave was decreased. Especially, it declined to ~77% after autoclaving for 30 min and 35% after both autoclaving for 50 min and microwaving for 5 min. In addition, the intensity of gliadin bands in SDS-PAGE were weakened and anti-gliadin IgG did not recognize gliadin in immunoblotting. However, microwaving alone did not affect the antigenicity of gliadin in wheat dough. These results indicate that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat dough. Moreover, autoclaving in combination with microwaving is more effective for reducing the antigenicity of wheat dough.

가압가열 및 Microwave 처리가 생면의 품질에 미치는 영향

박시우,김꽃봉우리,김민지,강보경,박원민,김보람,박홍민,최정수,최호덕,안동현,Bark, Si-Woo,Kim, Koth-Bong-Woo-Ri,Kim, Min-Ji,Kang, Bo-Kyeong,Pak, Won-Min,Kim, Bo-Ram,Park, Hong-Min,Choi, Jung-Su,Choi, Ho-Duk,Ahn, Dong-Hyun 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4

중력분을 이용하여 제면한 후 가압가열, microwave 및 가압가열과 microwave 병행 처리한 후, 생면 및 삶은 면의 품질에 미치는 영향에 대하여 알아보았다. 생면의 pH는 microwave 1 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았으며, 수분 함량은 autoclave 50 min 처리구에서 무처리구와 유의적인 차이를 보이지 않았고, 그 외의 처리구에서는 모두 수분 함량이 감소함을 확인하였다. 면의 색도 측정 결과는 명도는 무처리구에 비해 모든 처리구에서 감소하였으며, 적색도는 microwave 1 min 처리구는 감소한 반면, 나머지 처리구는 증가하였다. 황색도는 autoclave 50 min 처리구와 autoclave 50 min/microwave 1 min 병행 처리구에서 증가한 반면, 나머지 물리적 처리구에서는 감소하였다. 삶은 면의 색도에서는 명도, 적색도, 황색도 모두에서 무처리구에 비하여 물리적 처리구에서 증가한 값을 나타내었다. 물성 측정 결과는 경도, 부착성, 응집성, 검성, 복원성에서 무처리구와 비교시 microwave 1 min 처리구는 유의적으로 감소하였으며, 그 외 처리구에서는 유의적으로 증가한 값을 나타내었다. 탄력성과 전단력은 모든 처리구에서 유의적으로 증가하였으며, 인장력은 가압가열 30 min 처리구와 무처리구가 유의적인 차이를 보이지 않았으나, 나머지 물리적 처리구에서는 유의적으로 증가하였다. 관능 평가 결과는 생면의 경우 색 항목에서 물리적 처리구들이 유의적으로 낮은 점수를 받았다. 맛, 질감, 향 항목에서는 무처리구와 물리적 처리구간 유의적 차이를 보이지 않았다. 전체적인 기호도에서는 microwave 1 min 처리구가 가장 높은 점수를 얻었으며, 가압가열(50 min)과 microwave(1 min) 병행 처리구는 무처리구와 비슷한 점수를 얻었다. 따라서 제면 후 가압가열 및 microwave 처리가 생면 및 삶은 면에 있어서 색과 질감 항목을 보완, 개선시킨다면 알러겐성이 저감화된 면을 제품화하는데 적합할 것으로 사료되어진다. This study was conducted to determine the effects of physical treatments for quality of wet noodles. Noodles were being tried with a microwave (for 1 min), an autoclave (for 30 or 50 min), and both autoclave and microwave (for 30/1 min or 50/1 min). The results showed that the pH levels were slightly decreased after treatments of autoclave and autoclave/microwave. The moisture contents were considerably decreased as compared to the control except autoclave (50 min). After all treatments, the lightness was decreased in all samples, but, redness was increased (except microwave) and the yellowness was increased after autoclave (50 min) and autoclave/microwave (50/1 min). Texture was increased as compared to the control except microwave. In the sensory evaluation, the noodles treated with microwave, autoclave (50 min), and autoclave/ microwave (50/1 min) showed a high score in overall preference. From these results, both the autoclave and microwave methods can be applied to the wet noodles without diminishing its quality to a great extent.

서울의 Penicillinase Producing Neisseria Gonorrhoeae 발생빈도(1996)

김재홍,황동규,전재홍,김윤석,김중환,김용준,이창균,임동진,김현수,조창근,김경문,박상훈,전우형,김희성,이호정,차명수,김갑형,김형석,김석우,황지환,박병순,권오상,이민수,송기훈,성소영,이인섭,부태성 대한화학요법학회 1999 대한화학요법학회지 Vol.17 No.2

Background : In recent years, gonorrhea has been panedemic and remains one of the most commom STDs in the world, especially in developing countries. Objective & Methods: For the detection of a more effective therapeutic regimen and assessing the prevalence of PPNG, we have been trying to study the patients who have visited the VD Clinic of Choong-Ku Public Health Center in Seoul since 1980 by means of the chromogenic cephalosporin method. Results: In 1996, 139 strains of N. gonorrhoeae were isolated, among which 53(39.0%) were PPNG. Conclusion: Our results suggests that after a peak of 74.3% in 1993, the prevalence of PPNG in Seoul is gradually declining.

Effects of Atipamezole and Naloxone on Electroencephalographic Spectral Edge Frequency 95 in Dogs Sedated by Acupuncture at GV20 and Yintang Point

KIM, Min-Su,SEO, Kang-Moon Japanese Society of Veterinary Science 2007 The Journal of veterinary medical science Vol.69 No.5

<P>The purpose of this study was to evaluate the antagonism of acupuncture-induced sedation by electroencephalographic spectral edge frequency (SEF) 95 in 10 healthy intact male Miniature Schnauzer dogs (4.2-6.1kg; 2-3 years old) without neurological disorder. The GV20 and Yintang acupoints were administered for 20 min. While the dogs were conscious, SEF 95 baseline values were recorded at 2-min intervals for 4 min. Then acupuncture was administered at the GV20 and Yintang acupoints. During the acupuncture procedure, the SEF 95 values were recorded at 2-min intervals for 10 min. Subsequently, antagonist drugs, naloxone and atipamezole, were administered through the cephalic vein. The SEF 95 values were then measured again at 2-min intervals for 10 min. Those values were found to be significantly increased after administration of atipamezole in dogs sedated by acupuncture at the GV20 and Yintang acupoints (<I>p</I><0.05). However, the SEF 95 values in the naloxone groups did not show any significant changes before and after administration of the antagonist. It was concluded that sedation induced by acupuncture at the GV20 and Yintang acupoints might be partially associated with the α<SUB>2</SUB>-adrenergic system.</P>

Determining the Optimal Cooking Time for Cooking Loss, Shear Force, and Off-Odor Reduction of Pork Large Intestines

Sung-Su Kim,Yee Eun Lee,ho Hyun Kim,Joong-Seok Min,Dong Gyun Yim,Cheorun Jo 한국축산식품학회 2022 한국축산식품학회지 Vol.42 No.1

The objective of this study was to determine the optimal cooking time by considering the cooking loss, shear force, and off-odor reduction of pork large intestines. Commercial pork large intestines were purchased, quartered perpendicularly, and cooked in boiling water for 40, 120, 180, and 240 min. Cooking loss of the samples increased after 240 min of cooking (10.92, p<0.05) while shear force value was lower at 240 min (4.45) compared to that at other cooking times (p<0.001). The amount of major volatile organic compounds showed a decreasing trend with increasing cooking time. In particular, the amount of methyl pentanoate (17,528.71) and methyl isobutyrate (812.51), compounds with a relatively low odor threshold, decreased significantly after 120 min of cooking and no change was observed thereafter (p<0.05). In addition, the amount of 2-pentanol (3,785.65) and 1-propanol (622.26), possibly produced by lipid oxidation, significantly decreased at the same cooking time (p<0.001). In the principal component analysis, only the 40 min cooking time was significantly different from other cooking time by high amounts of 1-propanol, 2-pentanol, and methyl isobutyrate. In conclusion, in the present study, the optimal cooking time for pork large intestines was 120 min in terms of off-odor reduction, cooking loss, and shear force.

‘해방’의 전통과 복원 - 김학철을 중심으로

김민수 ( Kim¸ Min-su ) 한국문학언어학회 2021 어문론총 Vol.89 No.-

이 글은 조선의용군 출신 작가 김학철(金學鐵, 1916~2001)의 서지를 보완하기 위하여 작성된 것이다. 일차적으로는 김학철이 독립운동가에서 작가로 변모했던 해방 직후의 불완전한 서지를 갈무리하고자 하였다. 월북 이전의 작품목록을 정리하는 과정을 통해 당시의 정치지형을 검토할 수 있게 되었다. 더 나아가 김학철이 조선의용군을 형상화한 작품이 ‘해금’을 전후한 시기에 어떠한 방식으로 복원된 것인지 살피고자 하였다. 1980년대 민주화의 열기와 맞물려 ‘해방전후사(解放前後史)’는 출판문화계의 지대한 화두로 자리했다. 특히 중국에서 출판된 김학철의 전기문학 『항전별곡』(1983)과 『격정시대』(1986) 등이 일본을 경유하는 방식으로 한국에서 해적판으로 출판된 정황을 추적하고자 하였다. 이때 그의 소설은 민족해방운동에 관한 ‘사료’로 취급된 바 있을 정도이다. 이처럼 규율화된 폭력(Gewalt)을 무릅쓰고 우리에게 돌아온 조선의용군 서사는 ‘잔존(Survivance)’의 가치를 논의하기에 충분한 것이라 할 수가 있다. ‘해방’과 ‘해금’ 사이의 공차를 저울질하는 작업을 통하여 반세기에 걸쳐 금압의 역사로 자리했던 ‘해방공간’의 의미를 재구할 수 있을 것이라 기대한다. This article attempts to fill out the bibliography of Kim Hak-cheol (1916-2001), author and member of the Korean Volunteer Army. First, I organize incomplete bibliographic information from the period following the Liberation, when Kim shifted from independence movement activist to author. In organizing Kim’s list of works prior to his defection to North Korea, I also investigate the political geography of the period. I then examine how Kim’s depiction of the Korean Volunteer Army was restored around the time of the ‘lifting of the ban.’ In step with the fervor of democratization in the 1980s, ‘history at the time of liberation’ became an immensely important topic in the publishing world. In particular, I trace the circumstances under which Kim’s biographical works Hangjeonbyeolgok (1983) and Gyukjeongsidae (1986), originally published in China, were published in pirate editions by way of Japan. At this time, Kim’s novels were treated mostly as historical resources on the national liberation movement. Overcoming disciplinary violence (gewalt) to return to South Korean readers, this Korean Volunteer Army narrative offers an important chance to discuss the value of ‘survivance.’ By measuring the difference that appears between the ‘liberation’ and the ‘lifting of the ban,’ it is possible to begin reconstructing the meaning of the ‘Liberation Period,’ which occupied the position of suppressed history for half a century.

<개문인시폐문인(開門人是閉門人)> 유형 설화에서 문과 환생의 의미

김민수 ( Kim Min-su ) 온지학회 2023 溫知論叢 Vol.- No.76

본 논문은 <개문인시폐문인>유형 설화에 대한 새로운 해석을 시도하였다. 그간 다양한 설화에 등장하는 환생 모티프는 현실에서 불가능한 것들을 가능하게 하는 서사적 당위성을 확보하기 위한 장치로 활용되어 왔다. 그러나 <개문인시폐문인>유형 설화는 그간 본격적으로 분석되지 않았으며 환생의 의미가 기존과 다르다는 점에서 연구대상으로 가치가 있다. 본 논문에서는 해당 유형 설화에 주인공으로 왕수인이 반복적으로 등장한다는 사실에 집중하여 그 이유를 규명하고자 했다. 이를 위해 당시 왕수인이라는 인물이 불교에 관대한 경향을 가진 인물로서 갖는 위상을 확인하고 불교에 우호적이라는 속성이 어떻게 환생 모티프가 사용된 설화에 주인공으로 정착하는 이유가 되었는지를 설명했다. 이후 <개문인시폐문인>유형 설화의 의미 분석을 위해 구조주의적 분석 방식인 순차구조와 대립구조를 중첩하여 적용했다. 순차구조 분석을 통해 서사의 진행 과정이 형성하는 의미와 그 과정에서 ‘문’이라는 화소가 갖는 중요성을 파악했다. 그리고 이를 통해 고립에서 확장을 거쳐 통합에 이르는 왕수인의 상태 변화를 확인했다. 문을 기점으로 자연스럽게 형성된 대립구조를 통해서는 문을 중심으로 나뉜 방 안과 밖의 의미가 개인의 심리적 공간 내/외부를 의미한다는 사실을 밝혀냈다. 또 왕수인의 상태가 환생을 매개로 죽음과 삶을 연결하고 있다는 구조를 파악했다. 밝혀진 구조를 중심으로 문을 닫고 들어선 왕수인과 밖에서 열 수 없던 문이 원님에 의해 열리는 것이 의미하는 바 역시 분석했다. 결과적으로 <개문인시폐문인>유형 설화는 문제해결 과정에서 발생하는 심리학적 자기성장과 문제해결에서 주체의 해결의지가 갖는 중요성을 의미화하고 있다. 이를 통해 <개문인시폐문인>유형이 어떤 의미를 갖는지 새로운 해석을 제시할 수 있게 되었으며, 그 의미가 현대인들에게 존재 변화를 위한 고민의 가치와 지향점에 대해 시사하는 바를 확인할 수 있었다. This study attempts a new interpretation of the < Gaemuninsipyemunin >-type folktales. So far, the reincarnation motif that appears in many tales has been used as a device to secure narrative rightfulness that makes something impossible possible in reality. However, < Gaemuninsipyemunin >-type folktales have not been analyzed in earnest up until now, and they are valuable as a research subject in that the meaning of reincarnation is different from the previous one. In addition, focusing on the fact that Wang Su-in appears repeatedly as the main character in the type folktales, this study attempts to find out the reason. This researcher has figured the status of Wang Su-in as a person with a tendency to be generous about Buddhism within the social context then and explained how the attribute of being favorable to Buddhism was the reason for settling him as the main character in the folktales employing the reincarnation motif. Through that, this study has analyzed how Wang Su-in settled as the main character in the tales and was accepted by the class enjoying them. Next, to analyze the meaning of < Gaemuninsipyemunin >-type folktales, the sequential structure and confrontational structure which are the methods of structuralist analysis are applied in combination. Through sequential structure analysis, this study has figured the meaning that the narrative progress forms and the importance of the theme called ‘Mun (the door)’. Through that, the change in Wang Su-in’s state from isolation to extension and to integration has been observed. Through the confrontational structure naturally formed with the door, the fact that the meaning of the inside and outside of the room separated by the door refers to the meaning of the inside and outside psychological space of an individual has been revealed. Also, the structure that Wang Su-in’s state connects death and life with the mediation of reincarnation has been identified. Based on the structure revealed, this author has analyzed the meaning of Wang Su-in entering the room closing the door and the door opened by Wonnim although it had not been able to be opened outside. < Gaemuninsipyemunin >-type folktales signify psychological self-growth that occurs in the process of solving problems and the importance of the subject’s willingness to solve problems. This study is significant in that it has presented a new interpretation about the meaning of < Gaemuninsipyemunin >-type folktales loved by various classes through oral and written transmissions and figured what they imply for contemporaries about the value and direction of concerns for change in existence.

Solar urticaria in Korean patients

( Sung Min Kim ),( Young Ah Cho ),( Ji Su Lee ),( Ji Youn Hong ),( Jin Hee Kim ),( Joo Ran Hong ),( Hye In Cheon ),( Yang Won Lee ),( Yong Beom Choe ),( Kyu Joong Ahn ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: Solar urticaria (SU) is an uncommon type of photodermatoses characterized by erythematous wheal with pruritus after sunlight exposure, which shows a female predominance. Only a few studies to analyze the clinical features of SU were reported in Asia. Objectives: We aimed to analyze the clinical and photobiological characteristics of SU in Korea. Methods: We retrospectively investigated the medical records of patients with SU who visited the department of dermatology, Konkuk university medical center from January 2005 to January 2020. Results: Of a total of 44 patients, 36 (81.8%) were male and 8 (18.2%) were female. The mean age at onset was 17.6 years (range, 5-47) and the mean threshold time was 19.7 minutes. Among 32 patients who underwent phototesting, the action spectrum most commonly implicated was ultraviolet (UV) A only in 12 (37.5%), and then visible light (415nm) only was responsible for SU in 8 (25.0%). Three patients (9.4%) were induced by both UVA and visible light (415nm). Even 4 patients (12.5%) were triggered by only natural sunlight. Serum immunoglobulin E levels were measured in 33 patients, and 17 (51.5%) showed values higher than normal. The treatment most widely used were oral antihistamine (43.2%), followed by phototherapy (13.6%). Conclusion: SU is a rare photodermatosis in Korea, which shows a male preponderance. In this study, UVA and visible light (415nm) were predominant action spectrum of SU.

Reduction of Bacillus cereus Contamination in Biofilms on Stainless Steel Surfaces by Application of Sanitizers and Commercial Detergent

Lee, Min-Jeong,Ha, Ji-Hyoung,Kim, Yong-Su,Ryu, Jee-Hoon,Ha, Sang-Do The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1

We established the effectiveness of a washing, detergent and sanitizer treatments for reducing Bacillus cereus (B. cereus) contamination in biofilms on stainless steel surfaces. A water treatment did not affect biofilms and chlorine treatments at 200 ppm for 10 min and 300 ppm for 5 min, iodophor at 200 ppm for 10 min, and QAC at 400 ppm for 10 min were used to eliminate all B. cereus cells. B. cereus cells after detergent treatment were eliminated by chlorine at 100 ppm for 10 min, 200 ppm for 5 min, and 300 ppm for 3 min, and with iodophor at 100 ppm for 10 min and 200 ppm for 10 min, and QAC at 400 ppm for 10 min.

정상면역을 가진 성인에서 발생한 식도 방선균증

김현수 ( Hyun Soo Kim ),천종운 ( Jong Woon Cheon ),김민수 ( Min Su Kim ),정창길 ( Chang Kil Jung ),김경록 ( Kyung Rok Kim ),최재원 ( Jae Won Choi ),강동우 ( Dong Woo Kang ),김선영 ( Sun Young Kim ) 대한소화기학회 2013 대한소화기학회지 Vol.61 No.2

Hyun Soo Kim, Jong Woon Cheon, Min Su Kim, Chang Kil Jung, Kyung Rok Kim, Jae Won Choi, Dong Woo Kang and Sun Young Kim1 Departments of Internal Medicine and Pathology1, DongKang Medical Center, Ulsan, Korea Actinomycosis is a chronic suppurative disease and caused by Actinomycosis species, principally Actinomyces israelii, which are part of the normal inhabitant on the mucous membrane of the oropharynx, gastrointestinal tract, and urogenital tract. It usually affects cervicofacial, thoracic and abdominal tissue. Cervicofacial type has the highest percentage of occurrence with 50%. Actinomycosis frequently occurs following dental extraction, jaw surgery, chronic infection or poor oral hygiene. It may also be considered as an opportunistic infection in immunocompromised patients such as malignancy, human immunodeficiency virus infection, diabetes mellitus, steroid usage or alcoholism. But, actinomycosis rarely occurs in adults with normal immunity and rare in the esophagus. We report an unusual case of esophageal actinomycosis which was developed in a patient with normal immunity and improved by therapy with intravenous penicillin G followed oral amoxicillin, and we also reviewed the associated literature.