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여러 종류의 식품에서 Salmonella 균주를 손쉽고 빠르게 검출하기 위하여, Salmonella 장독소 유전자(s수)를 기초로 제작한 primer (STN1, STN2)를 이용하여 PCR을 수행한 결과 617 bp의 특이적인 DNA 단편을 얻을 수 있었다. PCR 민감도는 순수 배양한 균체에서 추출한 template DNA는 1 pg까지 검출이 가능하였고, 직접 균체를 template로 이용한 경우에는 10² cells까지 검출이 가능하였다. Salmonella typhimurium을 인위적으로 접종시킨 식품에서는 식품 1g당 10³~10⁴cells까지 검출할 수 있었다. 이러한 결과는 PCR을 이용하여 Salmonella에 오염된 식품에서 이들 균주를 간편하고 신속하게 검출할 수 있을 것이다. This study was carried out to investigate the simple and rapid detection of Salmonella species in different kinds of food using PCR method. The specific primer sets (STN1 and STN2) was designed and utilized to amplify a 617 bp DNA fragment from Salmonella species. The sensitivity of PCR was 1 pg of purified template DNA or 10² cells from pure culture. The detection limit of Salmonella typhimurium on agarose gel electrophoresis was 10³~10⁴cells/g in the artificially contaminated food samples. These results suggested that this simple method could be applied to industrial fields for detection of Salmonella species in food.
임베디드 시스템 장치에서 사용자에게 정보를 제공하고 사용자의 입력을 받기 위한 언터페이스의 중요성은 인터넷의 발전 및 휴대 전화 PDA 등과 같은 개인 정보 접속 장치의 대중화로 점차 커져가고 있다. 개인 정보 접속 장치뿐만 아니라 산업 현장에서 사용되고 있는 임베디드 시스템에서도 장치 제어 및 감시에 사용될 GUI의 요구 증가는 계속 되고 있다. 하지만 임베디드 장치의 제어 및 감시를 위한 GUI 라이브러리의 개발은 아직 미비한 실정이다. 따라서 임베디드 시스템 장치를 제어하고 감시하기 위한 기존의 GUI 라이브러리 보다 더 발전된 Gill 라이브러리를 설계하고 개발하였다. 그리고 새로운 GUI 리아브러리를 이용한 데모 프로그램을 작성하였다.
We encountered one case of Chinese Herb Nephropathy in Korea. But clinical feature of our case was different from those of CHN in Belgium. The purpose of this case report was clarified the features of CHN in Asia. The subjects consisted of a patient diagnosed as interstitial nephritis in Hanyang University Hospital and of those reported in the literature in Asia and Belgium. We investigated the clinical and histological features of CHN patients in Asia and compared them with the Belgian cases. The renarkable differences were as follows; (1) relatively high prevalence in males compared with Belgian cases, (2) digestion with multiple object and mode in Asia, (3) Most of renal failure in Asia were improved or were in stable status, (4) Fanconi's syndrome was found in most cases of Asia. In conclusion, CFN in Asia has some characteristics distinguished from Belgian Chinese Hreb Nephropathy. These findings could indicate that susceptibility to aristolochic acid may be different among races. Furthermore, it is likely that different components of AA could cause different features, that the amount of ingested AA, mode in digestion, or interaction with other components except nephrotoxic agent such as AA might reflect clinical pictures. Other hypothesis may be some other toxic substances affecting the clinical findings although they are not identified at present. further studies must be underaken to clarify these diferences.
This research overcomes limitation of physical model using storage function method that is used in flood routing method of flood forecasting-warning system of 5 basic principle basin of our country. We also developed a nonlinear-model of rainfall-runoff process using artificial neural networks (ANNs) that do not consider to hydrology structure of basin. The results show that the ANN_SA_9 selected in the present study generally well-performed the one-step ahead prediction of runoff in the study area, the Sunam station. Also, base on ANN_SA_9 which have the value of R2 more than 0.95 developed in this search, we can have a higher reliability to operate and manage the water resources and rivers plan more efficiently in the future.
During the last few years, the use of prefabricated concrete pile has been gradually increased in many construction sites. Cutting work of the concrete pile is an important task to crush a part of pile head which is compressed with more than 800㎏/㎠, It is usually performed by a crusher and three to four skilled workers. Recent analysis results of the pile cutting work reveal that it frequently makes a lot of cracks which significantly reduce the strength of the pile and is labor-intensive work. The primary objective of this study is to propose conceptual designs for developing an automated pile cutting machine. It is anticipated that the development of the automated pile cutting machine would be able to bring improvements in safety, productivity, quality as well as cost saving.
Aging is characterized by an age-dependent reduction in bone density. Pronounced bone loss by postmenopausal estrogen deficiency induces a bad effect on dynamic balance of bone metabolism. The purpose of this study is to examine what are the effects of the Ssangbohwan(SBH) on the ovariectomized rat model of postmenopausal osteoporosis. The results were summarized as follows : 1. Body weight in control group showed significant increase in comparison with sham, but that in SBH-treated showed no change in comparison with control. 2. The level of serum albumin in control group showed significant decrease in comparison with sham. That in SBH-treated was slightly decreased in comparison with control, which showed no efficacy. 3. The level of serum ALP activity in control group showed significant increase in comparison with sham, but that in SBH-treated was slightly decreased in comparison with control, which showed no efficacy. 4. The level of serum phosphorous in control group showed significant increase in comparison with sham, but that in SBH-treated showed no change in comparison with control. 5. The level of serum calcium in control group showed no change in comparison with sham, but that in SBH-treated was significantly decreased in comparison with control. 6. The level of serum T3 in control group showed no change in comparison with sham, but that SBH-treated showed significant decrease in comparison with control. 7. The level of serum T4 in control group showed significant increase in comparison with sham, and that in SBH-treated showed significant decrease in comparison with control. 8. Trabecular tibia strength in control group showed significant decrease in comparison with sham, but that in SBH-treated was slightly imcreased in comparison with control, which showed no efficacy. According to the above these results, SBH has shown to be capable of preventing and curing osteoporosis caused by old-aged and postmenopause