HMG-CoA 환원효소 억제제에 의한 ICAM-1 유전자의 발현조절

김현진,정효균,홍우정,김군순,조영석,김도희,채수흥,구본정,송민호,노흥규,김영건 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : ICAM-1 act as one of major adhesion molecules in the atherosclerotic lesion. ICAM-1 expression is mainly regulated at the level of transcription and depend on IFN-γ signal transduction pathway in which the STAT1 transcrption factor is a critical intermediate. IFN-γreceptor not only initiates tyrosine 701 phosphorylation of STAT1 by Jak1 and Jak2, but also phosphorylates serine 727 through the activation of Raf-1/MAP kinases. HMG-CoA reductase inhibitors have anti-atherosclertic effects, beyond normalization of hypercholesterolemia, by directly acting on endothelial cells, macrophages and vascular smooth muscle cells. HMG-CoA reductase inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibitors suppress the synthesis of isoprenoid intermediates such as geranylgeranyl-pyrophosphate or farnesylpyrophosphate. These effects results inhibition posttranslational farnesylation and geranyl-geranylation processing of small GTP-binding preoteins and inhibition of normal signaling activities. Method : We made several 5'-deletion constructs of rat ICAM-1 promoter and analyzed the promoter activities by measuring the luciferase activity after transfection into ECV304 cells and smooth muscle cells. We checked the level of total and phosphorylated STAT1 protein by immunoblot analysis using specific antibodies. Results : Lovastatin inhibits IFN-γ-induced ICAM-1 gene expression in the ECV304cell. The cells pretreated with PD98059, MEKK inhibitor showed significantly low ICAM-1 RNA induction with IFN-γ stimulatio. IFN-γ induced phosphorylation of tyrosine 701 was not significantly changed by the pretreatment of lovastatin. But lovastatin suppresses IFN-γ-induced phosphorylation of ERK1/ERK2 which are responsible for the seine 727 phosphorylation in STAT1. Conclusion : We showed that HMG-CoA reductase inhibitors, lovastatin, suppresses IFN-γ mediated ICAM-1 gene expression through the inhibition of transcription. HMG-CoA reductase inhibitor suppresses IFN-γ-induced phosphorylation of serine 727 in STAT1 through the modulation of MAP kinases.

개에서 발생한 폐수종의 치료 증례

김유수,심재현,김건우,김지용,이병희,송근호,이영원,김덕환 충남대학교 수의과대학 동물의과학연구소 2004 動物醫科學硏究誌 Vol.12 No.-

A 9 months intact male American bull dog with chief complaints of dry cough, dyspnea and fever was referred to the Veterinary Teaching Hospital, Chungnam National University. Based on the findings of physical examination, radiological examination, and blood and blood chemical examinations, the present patient was diagnosed into pulmonary edema secondary to primary bacterial and aspiration pneumonia. The patient was hospitalized and treated with buscopan, cephazolin and furosemide for 1 week. His activity and appetite was returned to normal and thoracic radiographs revealed that Jung was recovered to the normal condition after treatment for 7 days. The present patient was a case with pulmonary edema showed favorable therapeutic responses to proper medication.

Proteomic Approach to Analyzing Flooding Stress in Soybean (Glycine max(L.) Merr.)

Kim,Tae-Seon,Cho,Seong-Woo,Lee,Jae-Eun,Kim,Yook-Han,Cho,Kun,Choi,Jong-Soon,Chung,Keun-Yook,Song,Beom-Heon,Jong,Seung-Keun,Kim,Hong-Sig,Woo,Sun-Hee 한국작물학회 2005 한국작물학회 학술발표대회 논문집 Vol.2005 No.-

운명과 원한

김건우(Kim Kun Woo) 서강대학교 인문과학연구소 2018 서강인문논총 Vol.0 No.52

1943년 일본과 조선의 대학생들에게 징병이 예고되었을 때, 조선인 학생들의 반응은 일본인 학생들과는 큰 차이를 보였다. 일본인 학병들에게도 자신의 죽음에 이유가 필요했지만, 대체로 그들은 자신들이 처한 상황을 ‘운명’으로 받아들였다. 이와 달리 조선인 학생들은 ‘국가를 위한 죽음’을 받아들이지 못했고, 이에 따라 도피, 자기 방기 등 온갖 형태의 이상 반응을 보였다. 조선인 학생들은 자신의 처지를 비관했고, 그 모든 고통을 조상과 윗세대에 대한 ‘원한’의 형태로 간직했다. 그런데 태평양전쟁기 일본인 학생들에게 존재하던 ‘국가’라는 매개항이, 전쟁이 끝난 후 조선인들에게도 부활한다. 그 부활한 국가란 어떤 성격의 국가인가. ‘원한’을 세대론적으로 장착하고, 욕망을 투사할 새 ‘대상’은 어떤 성격을 지녀야 하는가. 이것은 좌우 이념의 문제이기도 했다. 문학사를 놓고 이야기할 때, 그 대답의 자리에 선우휘와 이병주가 있었다. 선우휘와 그의 소설 『불꽃』의 모델 신상초는 대한민국이라는 국가를 자기 운명과 등치시킬 수 있었다. 이와는 달리, 이병주와 그의 모델 황용주가 남한에서 자신의 운명을 발견할 방법이란 없었다. 이념의 경계에 서성이는, ‘이방인’으로 살아가는 것이 그들이 할 수 있는 전부였다. When the empire of japan announced in 1943 that the military draft would be enforced on college students, the reactions of korean students were different from those of japanese students. Japanese students mostly accepted their situations as ‘fates’, though they also felt the need of excuses for their dying. But korean students could not embrace ‘dying for the sake of the state’, and exhibited various types of abnormal reactions such as escape, self-abandonment, etc. They felt deeply hopeless and desperate about their situations, and kept these feelings inside as a form of resentment against their ancestors. But a state - a kind of mediator - which had existed for Japanese students during the pacific war, was resurrected after the war for Korean students too. What was supposed to be the characteristic of this state resurrected? What characteristic should be attached to this new ‘object’ toward which they would project their desires keeping resentment in mind as a collective emotion of the student soldier generation? This question was also concerned with rightist and leftist ideologies. Seen from the perspective of korean literary history, Sun Woo-Hui and Lee Byung-Joo were two versions of the answer to this question. Sun Woo-Hui and Shin Sang-Cho, the model of Sun"s short story Flowers of Fire identified their fates with the fate of the state, ie. the republic of korea. On the contrary, Lee Byung-Joo and his model, Hwang Young-Joo could not find their fates in south korea. There was nothing they could do except wavering along the border of ideologies and living as ‘aliens’.

논문 : 월남 학병세대의 해방 후 8년 -학병세대 연구를 위한 시론(試論)

김건우 ( Kun Woo Kim ) 민족문학사학회·민족문학사연구소 2015 민족문학사연구 Vol.57 No.-

일제 말 제국의 고등교육을 받은 학병세대는 한국 현대지성사에서 가지는 중요성에도 불구하고 이제까지 연구가 거의 이루어지지 않았다. 해방 직후 이들은 이미 주목을 받고있었으며, 이후 한국사회 각 분야에 진출하여 국가를 설계하고 체계를 완성해 냈다. 1917-1923년에 태어나 해방 후 8년의 기간을 통해 ``남``을 선택한 월남 학병세대의 생각과 실천들을, 장준하 김준엽 선우휘 강원용 등 대표적인 월남 학병세대의 인물들을 통해 살펴봄으로써 향후 학병세대 연구의 기초를 만들고자 한다. While the student-soldier generation who were highly educated in the late japanese imperial period has considerable significance in the korean modern intellectual history, they have hardly been studied yet. In fact, they already held the public eye right after the liberation, and thereafter entered into various fields of korean society and designed the foundation of the state, completing the system of korea. This thesis tries to pave the way for the future studies on the student-soldier generation by examining the thoughts and practices of the ``Wollammin(persons displaced from North Korea to South Korea)`` student- soldier generation who were born between 1917-1923 and chose the ``South`` during the eight years after the liberation, especially those of the important ``Wollammin`` student- soldier generation such as Jang Jun-Ha, Kim Jun-Yeop, Seon Woo-Hui, Kang Won-Yong.

국학, 국문학, 국사학과 세계사적 보편성 : 1970년대 비평의 한 기원

김건우(Kim Kun-Woo) 한국현대문학회 2012 한국현대문학연구 Vol.0 No.37

It is generally accepted that 1970s' criticisms are best approached with the notion of the confrontation of Munhak-kwa-Jisung versus. Chanjak-kwa-Bipyung, but that approach could erase a lot of momenta latent in the intellectual history of 1970s. This thesis investigates the spiritual common basis of so-called 4.19 generation critics who had appeared on the scene not long before and soon would have their prime times. Dongil Cho's multi-volumed General History of Korean Literature (HanKukMunHakTongSa), which had been planned in mid-1960s and whose volume 1 was published in 1982, was indebted much to the rise of the theory of internal development and it is related debates about periodization in the circles of Korean History. This theory of internal development, mainly intended to overcome the colonial view of korean history(i.e. the notorious notions such as delay and heteronomy of korean history), was obsessed by method/attitude of the historiography. Hyun Kim's The History of Korean Literature(HanKukMunHakSa) was also included in this discourse field. There was the leftist motifs in Yongseop Kim's studies that had empirically founded the theory of internal development, but Nakcheong Back tried to realize the motifs not by historiography but by literary criticisms. He adopted the theory of internal development between 1966 and 1969, and developed his own theories of Citizen Literature/National Literature on the basis of it. There underlied the nationalistic longings to discover the universal motifs of world history(or western history) in the paradigm of 4.19 generation's criticisms which had a long influence on korean literature from the late 1960s. Despite the fact that there also existed the lefitist motifs in that aspirings, nationalism itself could not avoid the kinship with the state ideology and we can visibly see their relation made real in the korean contemporary history. This shows that whether it being historiography or literary criticism, 4.19 generation's writing, regardless of being leftist or rightist, could not escape from the category of Koreanology and paradoxically could not break out of the universalistic spell. 1970년대 비평을 '문학과지성' 대 '창작과비평'이라는 하나의 대립구도로 접근하는 오랜 통념은 당대 지식사의 수많은 계기들을 사상한다. 본고는 4.19세대로 묶일 수 있는, 1960년대 중반 이후 등장하여 얼마 되지 않아 그들의 시대를 가지게 되는 비평가들의 '공통된' 정신적 기반을 논하려는 것이다. 1960년대 중반부터 기획되어 1982년에 첫 권을 내게 된 조동일의 『한국문학통사』는 국사학계의 '내재적 발전론'의 대두와 이와 관련된 시대구분 논쟁에서 힘입은 바 컸다. 국사학계의 내재적 발전론이 식민사학의 정체성론, 타율성론을 극복하기 위해 성립된 것이었던 까닭에, '근대의 자생적 기점'을 거론하는 이러한 관점은 역사서술 방식/태도에 대한 강박으로부터 출발한 것이었다고 할 수 있다. 김현의 『한국문학사』 역시 이 자장에 포섭되었음은 물론이다. 내재적 발전론을 실증적으로 확립한 역사학자 김용섭에게서부터 사회경제사학 특유의 좌파적 계기가 존재했는데, 백낙청은 그 좌파적 계기를 문학사 서술이 아닌 비평을 통해 구현했다. 1966년에서 1969년 사이에 백낙청은 내재적 발전론을 수용하고 이에 입각해 이후 시민문학론, 민족문학론을 발전시켜 나가게 된다. 1960년대 후반부터 비롯되어 이후 오랜 기간을 통해 영향력을 행사한 4.19세대 비평 패러다임의 근저에는, 세계사적(서구적) 보편성을 한국사에서 찾고자 했던 민족주의적 열망이 자리하고 있었다. 이런 열망 속에 좌파적 계기가 존재했음에도 불구하고, 민족주의 자체가 국가 통치 이데올로기와 접점을 지니고 있을 수밖에 없었다는 점은 현대사를 통해 실증되는 사실이다. 이는 1970년대에 진입하던 4.19세대에 있어 문학사가 되었든 비평이 되었든, 좌우를 막론하고 소위 '국학'의 범주를 벗어날 수 없었음을, 역설적으로 보편성의 강박에서 풀려날 수 없었음을 의미하는 것이기도 하다.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

면역억제제를 사용치 않은 동소성 간이식 실험견의 조직병리소견

조원현,최순옥,김인호,임태진,김성수,박준희,김우석,김현철,최규택,김진모,권건영 大韓移植學會 1994 Korean Journal of Transplantation Vol.8 No.1

갑상선 세포에서 전사보조활성인자인 CBP와 CIITA의 인터페론-감마 활성 부위에 대한 조절 : CBP and CIITA on GAS(Interferon- γ Activated Site)-Mediated Transcription in Thyroid Cells

채수흥,홍우정,김군순,조영석,김도희,김현진,구본정,송민호,김영건,노흥규 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : In the previous stuides, we identified that the interferon-r activated sequence (GAS) in the 5-flanking region of rat ICAM-1 gene is major element for interferon-r-inducible expression of the gene in rat thyroid cells, FRTL-5. We here, investigated the role of transcriptional coactivators, CBP(CREB binding protein) and CⅡTA (class Ⅱ transactivator) in the modulation of the activity of GAS which could interacts with signal transducers and activators of transcription-1 and 3 (STAT1 and STAT3). Methods : The expression of CBP RNA and protein were quantitated in FRTL-5 after stimulation with interferon-r (IFN-r), thyroid stimulating hormone (TSH), forskolin and methimazole. Direct association of CBP with STAT were analyzed by immunoprecipitation. The transcriptional roles of CBP and CⅡTA in the regulation of GAS were assessed by the cotransfection with their expression vectors with reporters; 5-deletion constructs of rat ICAM-1 promoter or 8xGAS-luc constructs, into FRTL-5 thyroid cells. Results : The level of CBP RNA and protein were not changed by the treatment with TSH, IFN-r, forskolin and methimazole in FRTL-5, FRT and BRL liver cells. The CBP could be directly associated with STAT1. Furthermore, the overexpression of CBP significantly increases the both promoter activities; rat ICAM-1 gene promoter which has GAS element and 8xGAS-luc cassette constructs. However, the cotransfection of CⅡTA decreased the constitutive and CBP-mediated transactivation of rat ICAM-1 promoter and 8xGAS-luc cassette constructs. Conclusion : We identified that the two tanscriptional coactivators; CBP and CⅡTA has differential roles in the regulation of transcriptional activity of GAS drived promoter. CBP increases the GAS activity through the direct binding with STAT1, but CⅡTA inhibited the CBP-mediated transactivation of GAS activity.

종격동 종괴를 의심케한 우대동맥궁 1례

김만우,백학연,정춘해,장경식,홍순표,조건국,김종진 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Developmental anomalies of the aortic arch, commonly known as vascular rings, are relatively rare congenital vascular anomalies which can compress the trachea and esophagus. One routine chest film of a patient who was checked and hepatoma-diagnosed in Internal Medicine, Chosun University Hospital left the room for doubt of mediastinal mass. But no subjective symptoms for the mass was told by the patient. This is to report our experience of a patient having right side aortic arch with aberrant left subclavian artery, as proved by diagnosis, but none of congenital heart disease. This right side aortic arch with aberrant left subclavian artery is the most frequent type among right aortic arch, the anomaly of which is usually detected from the incidental suspicion for mediastinal mass at routine chest film; because no symptoms are accompanied therewith.