백서와 기니픽의 대뇌피질에서 Opioid Kappa 수용체의 특성에 관한 연구

김기원(Kee-Won Kim),노혜원(Hye-Won Rho),김형일(Hyoung-Il Kim),은재순(Jae-Soon Eun),소수미(Soo-Mi Soh),조규박(Kyu-Park Cho) 대한약리학회 1994 대한약리학잡지 Vol.30 No.2

In this study, we tested the influences of several κ opioid ligands on the [³H]diprenorphine binding in rat and guinea pig cortex membrane preparations. Using paradigm to block μ and δ opioid receptors with DAMGO(1μM) and DPDPE(1μM), [³H]diprenorphine labeled κ sites. Competition analysis in both rat and guinea pig cortex has shown a single population of [³H]diprenorphine binding site with different Kd values, respectively. There is a significant difference in Ki values of (-) WIN44441 and (+)WIN44441 in both rat and guinea pig cortex. Bremazocine, (-)ethylketocyclazocine, (-)cyclazocine, nor-binaltorphimine effectively inhibited the [³H]diprenorphine binding with different Ki values in rat and guinea pig cortex. U-69,593, U-50,488H and dynorphine-A (1-8) did not inhibit the [³H]diprenorphine binding in rat but in guinea pig cortex. Nor-binaltorphimine was a ligand discriminate the κ₁, and κ₂ receptor most effectively. We, also, examined the influence of Na ion and GTPγS, a nonhydrolyzable guanine nucleotide analog, on the inhibition of [³H]diprenorphine binding by diprenorphine, (-)ethyl-ketocyclazocine, U-69,593 and bremazocine. By the replacement of NaCl with N-methy-D-glucamine or addition of GTPγS, Ki values of diprenorpnine were not changed and that of ethylketocyclazocine were changed significantly in both rat and guinea pig cortex. The Ki value of bremazocine was decreased by removal of Na ion, and increased by GTPγS, however, was not changed by any one of either. These results suggest that there are 2 kinds of subtypes of κ opioid receptor, κ₁, and κ₂, showing different Ki values for various κ opioid ligands, also, bremazocine possess the antagonistic property at κ₂ site which is dominant subtype of K receptor in rat cortex.

권역심뇌혈관질환센터 사업의 비용-편익 분석

김병호 ( Byung Ho Kim ),감신 ( Sin Kam ),배상근 ( Sang Geun Bae ),김건엽 ( Keon-yeop Kim ),이원기 ( Won Kee Lee ),홍남수 ( Nam-soo Hong ),박형근 ( Hyeung-keun Park ),이혜진 ( Heyjean Lee ),조은정 ( Eun-jung Cho ),채성철 ( Shung C 한국보건정보통계학회 2017 보건정보통계학회지 Vol.42 No.1

Objectives: This study was conducted to investigate economic feasibility of operating Regional Cardiocerebrovascular Centers using cost-benefit analysis. Methods: The current study assessed cost and benefit data collected from nine hospitals which were designated as Regional Cardiocerebrovascular Centers between 2008 and 2010. Results: The operation of Regional Cardiocerebrovascular Centers reduced mortality rate and length of stay due to acute myocardial infarction and stroke and also decreased disability occurred after stroke. The total calculated cost of operating nine Regional Cardiocerebrovascular Centers was 19.85-22.30 billion won and the minimum benefit calculated was 23.98 billion won. Applying the maximum cost and the minimum benefit, the net benefit was 1.68 billion won, which is estimated to 190 million won per Regional Cardiocerebrovascular Center. Conclusions: The present study revealed that the operation of Regional Cardiocerebrovascular Centers was economically beneficial. The benefit would be larger if intangible benefit such as the long-term effects to community hospitals and enhancement of quality of life of patients and their families are considered.

흡연이 치과 외래 이용 및 치과 외래 의료비에 미치는 영향

정선락 ( Sun Rak Jeong ),김영희 ( Young Hee Kim ),장지언 ( Ji Eon Jang ),이원기 ( Won Kee Lee ) 대한예방치과·구강보건학회 2015 대한구강보건학회지 Vol.39 No.4

Objectives: The objectives of this study were to evaluate the effect of smoking on utilization of and expenses in ambulatory dental care. Methods: The subjects of this study were 5,751 men aged over 20 years selected from the Korea Health Panel data collected during 2010-2012. Pack-years of smoking were calculated based on the survey data. Dental utilization was defined as dental outpatient clinic use least once in three years. The expenses in ambulatory dental care were summed to determine total dental health care spending for three years. To detect the effect of smoking, we used multiple logistic regression analysis for dental utilization and multiple linear regression for expenses in ambulatory dental care after changing log. Results: The pack-years had a significant effect on dental utilization and expenses in participants aged 40-59 years. After controlling for income level, bedtime brushing, and the presence of chronic disease, dental care utilization rates in the over 10 to less than 25 pack-year and the over 25 pack-year groups were about 1.4 (OR=1.37, (P =0.007)) and 1.6 times (OR=1.59, (P =0.001)) those in the 0 pack-year group. In comparison with the 0 pack-year group, the over 10 to less than 25 pack-year group spent 50,000 won more over dental expenses, while the corresponding number for the over 25 pack-year group was about 100,000 won. Thus, the ambulatory dental care expenses increased with the packyears of smoking. Conclusions: Smoking significantly influenced the utilization and expense in ambulatory dental care in men aged 40-59 years.

SEPARATION OF A PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FROM CYTOSOLIC FRACTION OF SOYBEAN SPROUTS

Kim, Kee Hong,Kim, Jae Won,Shin, Yong Chul,Cho, Moo Je,Lee, Sang Yeol Plant molecular biology and biotechnology research 1992 Proceedings the 2nd Korean-Germany joint symposium Vol.1992 No.-

The Apoptotic Effects of Oxidative Stress and Antiapoptotic Effects of Caspase Inhibitors on Rat Notochordal Cells

Kim, Ki-Won,Ha, Kee-Yong,Lee, Jun-Seok,Rhyu, Kee-Won,An, Howard S.,Woo, Young-Kyun Lippincott Williams & Wilkins 2007 Spine Vol.32 No.22

STUDY DESIGN.: Western blotting and flow cytometric analyses were performed using rat notochordal cells. OBJECTIVE.: To demonstrate the apoptotic effect of oxidative stress and the antiapoptotic effects of caspase inhibitors on rat notochordal cells. SUMMARY OF BACKGROUND DATA.: Although oxidative stress causes apoptosis in many cell types, its effect on the apoptosis of notochordal cell and antiapoptotic effects of caspase inhibitors on the oxidative stress-induced apoptosis are unknown. METHODS.: Cultured rat notochordal cells were exposed to oxidative stress [500 μmol/L of hydrogen peroxide (H2O2)]. To determine the oxidative stress-induced apoptotic pathways, activations of caspases (-3, -8, and -9) as well as cleavages of Bid and poly (ADP-ribose) polymerase (PARP) were evaluated with Western blotting 6 hours after oxidative stress. To elucidate the antiapoptotic effects of caspase inhibitors on the oxidative stress induced-apoptosis, apoptotic rates of notochordal cells with or without treatment of specific caspase inhibitors (z-IETD-fmk for caspase-8, z-LEHD-fmk for caspase-9, and z-DEVD-fmk for caspase-3) were quantified by flow cytometry. RESULTS.: Oxidative stress significantly increased apoptosis of rat notochordal cells (2.1% vs. 4.75%, P = 0.008) and led to activations of initiators of intrinsic (caspases-9) and extrinsic (caspase-8) pathways as well as their common executioner (caspase-3). It also caused cleavages of Bid and PARP. Flow cytometric analysis showed that inhibition of only one of the intrinsic and extrinsic pathways by caspase-9 inhibitor (4.75% vs. 3.56%, P = 0.31) and caspase-8 inhibitor (4.75% vs. 5.24%, P = 0.84) did not significantly suppress the oxidative stress-induced apoptosis. However, inhibition of both pathways by caspase-3 inhibitor significantly reduced the oxidative stress-induced apoptosis (4.75% vs. 2.64%, P = 0.008) to the control level (2.1% vs. 2.64%, P = 0.15). CONCLUSION.: Oxidative stress caused apoptosis of rat notochordal cells via both intrinsic and extrinsic (Type I and Type II) pathways. Because caspase inhibitors are being used in clinical trials, inhibition of both pathways using caspase inhibitors might be of future therapeutic importance in oxidative stress-induced apoptosis of notochordal cells. Our results suggest that inhibition of inappropriate or premature oxidative stress-induced apoptosis of notochordal cells may delay the starting point of disc degeneration.

Paenibacillus elgii SD17 as a Biocontrol Agent Against Soil-borne Turf Diseases

Kim, Dal-Soo,Rae, Cheol-Yong,Chun, Sam-Jae,Kim, Do-Hyung,Choi, Sung-Won,Choi, Kee-Hyun The Korean Society of Plant Pathology 2005 Plant Pathology Journal Vol.21 No.4

Paenibacillus elgii SD17 (KCTC $10016BP^T$=NBRC $100335^T$) was recently reported as a new species. Based on its inhibitory activity to Thanatephorus cucumeris AG1-1, strain SD17 was further evaluated for its potential as a biocontrol agent against soil-borne diseases of turf grasses in Korea. P. elgii SD17 showed a broad spectrum of antimicrobial activity in vitro test and suppressed development of turf grass diseases; Pythium blight caused by Pythium aphanidermatum and brown patch caused by T. cucumeris AG1-1 on creeping bentgrass (Agrostis palustris) in the growth chamber tests. Under a condition for massive culture in a 5,000 L fermenter, P. elgii SD17 reached $6.4{\times}10^8$ spores/ml that resulted in approximately $1.0{\times}10^7$ cfu/g when formulated into a granule formulation (GR) using the whole culture broth instead of water. Using the GR formulation, biocontrol activity of P. elgii SD17 was confirmed. In the growth chamber tests, the GR formulation was effective against brown patch and Pythium blight with similar level of disease severity compared to each of the standard fungicides at the application rates of 10 g/$m^2$ or above. In the field tests, compared to each untreated control, the GR formulation also effectively controlled Pythium blight, brown patch and large patch at all the application rates of 5, 10 and 20 g/$m^2$, respectively, without significant response by the application rates. However its performance was inferior to each of the standard chemical fungicides. Based on these results, we consider this GR formulation of P. elgii SD17 as an effective biocontol agent to suppress Pythium blight, brown patch and large patch of turf grasses in Korea.

Near-infrared Polarimetric Study of the N159/N160 Star-forming Complex in the Large Magellanic Cloud

Kim, Jaeyeong,Jeong, Woong-Seob,Pyo, Jeonghyun,Pak, Soojong,Park, Won-Kee,Kwon, Jungmi,Tamura, Motohide American Institute of Physics 2017 The Astronomical journal Vol.153 No.3

<P>We present near-infrared polarimetric results for the N159/N160 star-forming complex in the Large Magellanic Cloud with SIRPOL, the polarimeter of the Infrared Survey Facility. We separated foreground sources using their visual extinction derived from near-infrared photometric data. The 45 young stellar candidates and 2 high-excitation blobs were matched with our sources, and 12 of them showed high polarization that did not originate from the interstellar dust. We made a polarimetric catalog of 252, 277, and 89 sources at the J, H, and K-s bands, respectively. Based on the ratios of the polarization degree between these bands, we verify that the origin of these polarized sources is the dichroic extinction from the interstellar dust aligned by the magnetic field and that the ratios follow a power-law dependence of P-lambda similar to lambda(-0.9). The linear polarization vectors projected onto the Ha image of the complex turned out to follow the local magnetic field structure. The vector map overlaid on dust and gas emissions shows the close correlation between the magnetic field structure and surrounding interstellar medium. We suggest that the derived magnetic field structure supports the sequential formation scenario of the complex.</P>

PHYSICAL SPECTRUM OF CHIRAL SCHWINGER MODEL IN THE MATTER GAUGE

Kim, Won Tae,Kim, Jae Kwan,Park, Young Jai,Kim, Kee Yong,Kim, Yong Duk 서울대학교 이론물리학연구센터 1992 연구논문집 Vol.1 No.-

Production of Japanese Encephatilis Virus Vaccine Using Plant Expression System

Won-Kyung LEE,Kiran BHILARE,Jun-Hye SHIN,Jin-Seo KIM,Juheon MOON,Jong-Won KEUM,Wipa TANGKANANOND,George LOMONOSSOFF,Kee-Jong HONG,Seong-Ryong KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

Argyrophilic Nucleolar Organizer Regions in Acute Lymphoblastic Leukemia

Kim, Myung-Shin,Lee, Seung-Ok,Lim, Ji-Hyang,Kim, Yong-Goo,Han, Kyung-Ja,Kang, Chang-Suk,Kim, Won-Il,Kim, Byung-Kee 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-

Background : In acute lymphoblastic leukemia (ALL), the importance of argyrophilic nucleolar organizer regions (AgNOR) is not established. Methods : NOR silver staining was carried out in 74 ALL patients. We analyzed the AgNOR parameters; counting parameters including number of AgNOR per cell, percentage of cells with one cluster, and area parameters including mean AgNOR area, total AgNOR area, and its percentage of nuclear area by morphometry. Cyclin A index was evaluated by immunohistochemical stain. We compared the AgNOR parameters with cyclin A index and evaluated the differences of AgNOR parameters in accordance with FAB classification, immunophenotype, a new classification of ALL (ALL with maturation), and response to induction chemotherapy. Results : A positive correlation was found between cyclin a index and AgNOR area parameters and a significant negative correlation was found between mean AgNOR area and number of AgNOR per cell (r=-0.433, P=0.000). AgNOR area parameters revealed the highest value in L3. The number of AgNOR per cell in T cell ALL was higher than non-T cell ALL (P=0.011), and the percentage of cells with one cluster was lower (P=0.002). In cases achieved complete remission(CR) after induction chemotherapy, the number of AgNOR per cell was higher (P=0.005) and the percentage of cells with one cluster was much lower than in cases failed to achieve CR (P=0.013). Conclusions : Our study indicates that the AgNOR area parameters are helpful to predict the proliferating activity of leukemic blasts in ALL. It is suggested that the number of AgNOR per cell and the percentage of cells with one cluster provide a valuable information to estimate the response to chemotherapy in ALL. (Korean Journal of Clinical Pathology 10:479-85, 1999)